Ecophysiological controls on water use of tropical cloud forest trees in response to experimental drought.

Tropical montane cloud forests (TMCFs) are expected to experience more frequent and prolonged droughts over the coming century, yet understanding of TCMF tree responses to moisture stress remains weak compared with the lowland tropics. We simulated a severe drought in a throughfall reduction experiment (TFR) for 2 years in a Peruvian TCMF and evaluated the physiological responses of several dominant species (Clusia flaviflora Engl., Weinmannia bangii (Rusby) Engl., Weinmannia crassifolia Ruiz & Pav. and Prunus integrifolia (C. Presl) Walp). Measurements were taken of (i) sap flow; (ii) diurnal cycles of stem shrinkage, stem moisture variation and water-use; and (iii) intrinsic water-use efficiency (iWUE) estimated from foliar δ13C. In W. bangii, we used dendrometers and volumetric water content (VWC) sensors to quantify daily cycles of stem water storage. In 2 years of sap flow (Js) data, we found a threshold response of water use to vapor pressure deficit vapor pressure deficit (VPD) > 1.07 kPa independent of treatment, though control trees used more soil water than the treatment trees. The daily decline in water use in the TFR trees was associated with a strong reduction in both morning and afternoon Js rates at a given VPD. Soil moisture also affected the hysteresis strength between Js and VPD. Reduced hysteresis under moisture stress implies that TMCFs are strongly dependent on shallow soil water. Additionally, we suggest that hysteresis can serve as a sensitive indicator of environmental constraints on plant function. Finally, 6 months into the experiment, the TFR treatment significantly increased iWUE in all study species. Our results highlight the conservative behavior of TMCF tree water use under severe soil drought and elucidate physiological thresholds related to VPD and its interaction with soil moisture. The observed strongly isohydric response likely incurs a cost to the carbon balance of the tree and reduces overall ecosystem carbon uptake.

Intra- and interspecific tree growth across a long altitudinal gradient in the Peruvian Andes.

Tree growth response across environmental gradients is fundamental to understanding species distributional ecology and forest ecosystem ecology and to predict future ecosystem services. Cross-sectional patterns of ecosystem properties with respect to climatic gradients are often used to predict ecosystem responses to global change. Across sites in the tropics, primary productivity increases with temperature, suggesting that forest ecosystems will become more productive as temperature rises. However, this trend is confounded with a shift in species composition and so may not reflect the response of in situ forests to warming. In this study, we simultaneously studied tree diameter growth across the altitudinal ranges of species within a single genus across a geographically compact temperature gradient, to separate the direct effect of temperature on tree growth from that of species compositional turnover. Using a Bayesian state space modeling framework we combined data from repeated diameter censuses and dendrometer measurements from across a 1700-m altitudinal gradient collected over six years on over 2400 trees in Weinmannia, a dominant and widespread genus of cloud forest trees in the Andes. Within species, growth showed no consistent trend with altitude, but higher-elevation species had lower growth rates than lower-elevation species, suggesting that species turnover is largely responsible for the positive correlation between productivity and temperature in tropical forests. Our results may indicate a significant difference in how low- and high-latitude forests will respond to climate change, since temperate and boreal tree species are consistently observed to have a positive relationship between growth and temperature. If our results hold for other tropical species, a positive response in ecosystem productivity to increasing temperatures in the Andes will depend on the altitudinal migration of tree species. The rapid pace of climate change, and slow observed rates of migration, suggest a slow, or even initially negative response of ecosystem productivity to warming. Finally, this study shows how the observed scale of biological organization can affect conclusions drawn from studies of ecological phenomena across environmental gradients, and calls into question the common practice in tropical ecology of lumping species at higher taxonomic levels.