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1.
Appetite ; 197: 107288, 2024 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-38467193

RESUMO

Market actors have a role to play in enabling sustainable food transitions. One challenge for these actors is how to promote plant-based foods in ways that appeal to a growing number of consumers. Here we test how different plant-based related labels affect consumer appraisals of a range of foods (cookies, sausages, cheese, chocolate, pasta). In two studies (pre-registered; NUSA = 1148, NGermany = 491), we examined the effects of a 'vegetarian', 'vegan', or 'plant-based' label (compared to no label) on five attributes (healthy, tasty, ethical, pure, environmentally friendly) related to the products. We also measured self-reported likelihood to purchase the products. Overall, the results indicated that the 'plant-based' label was slightly more appealing to participants than the 'vegetarian' and 'vegan' labels. However, contrary to our expectations, neither consumers' information-seeking tendencies nor their pre-existing attitudes toward plant-based foods influenced (i.e., moderated) the effects of the labels. Anticipated taste was a strong and consistent predictor of purchase likelihood for all labeled products, but the ethical and pure attributes also accounted for unique variance in this outcome variable. Taken together, our findings and discussion provide insights into the role of labels and label terminology on consumer appraisals of plant-based foods.


Assuntos
Cacau , Chocolate , Humanos , Veganos , Dieta Vegana , Atitude , Comportamento do Consumidor
2.
Public Health Nutr ; 26(11): 2619-2627, 2023 11.
Artigo em Inglês | MEDLINE | ID: mdl-37712383

RESUMO

OBJECTIVE: Shifting from meat-centric to plant-rich diets may help to enable healthier and more sustainable food systems. Here we present the results of a 1-week intervention to promote plant-based eating in a meat-centric food context (i.e. canteen). DESIGN: The intervention included environmental restructuring strategies (e.g. promotional materials and menu redevelopment) and improvements to the offer of plant-based meals. The evaluation (sales data; pre-registered) spanned 3 weeks prior to the intervention (baseline), 1 week during the intervention (immediate/short-term impact) and 3 weeks after the intervention (follow-up). Opinion surveys were also used to collect data with customers during the intervention. SETTING: Canteen unit of a university campus in Portugal (Lisbon metropolitan area). PARTICIPANTS: In addition to sales data (baseline: 7965 meals; immediate/short-term: 2635 meals; follow-up: 7135 meals), we used opinion surveys to assess customers' meal appraisals during the intervention (n = 370). RESULTS: The odds of a sold meal being vegetarian were 24 % higher in the intervention week compared with the pre-intervention period [OR = 1·24, 95 % CI (1·10, 1·40)] and 9 % higher in the post-intervention period compared with the pre-intervention period [OR = 1·09, (95 % CI (1·00, 1·19)]. Survey data showed that vegetarian meals compared favourably to meat and fish alternatives in liking, sustainability and satiety. CONCLUSIONS: A short-term, theory-driven, operationally feasible intervention was effective in promoting increased plant-based meal choices in a collective meal context. Nevertheless, these changes were not entirely sustained over time. Future studies could test whether prolonged or more transformative interventions are necessary to unlock entrenched food practices more effectively in meat-centric collective meal contexts.


Assuntos
Dieta , Carne , Humanos , Refeições , Universidades , Saciação
3.
Appetite ; 184: 106511, 2023 05 01.
Artigo em Inglês | MEDLINE | ID: mdl-36858261

RESUMO

Global environmental and public health challenges related to current food systems call for large-scale shifts towards increasingly plant-based diets, especially in Western meat-centric societies. School meal systems can play a role in these changes due to their widespread prevalence and multi-sectoral impact. However, there is a lack of evidence about how adults involved in the school meals system perceive school-based pro-environmental food policies, which limits the ability to align those policies with the needs and expectations of the school community. This study aimed to address this knowledge gap by exploring parents' (n = 104) and teachers' (n = 252) support for policies to promote increased plant-based eating in public schools in a highly meat-centric EU country (Portugal). Overall, teachers seemed to be slightly more supportive of such policies and displayed more favorable (injunctive and dynamic) norms toward plant-based eating, more negative appraisals of meals with meat (i.e., perceived healthiness, naturalness, and sustainability), and lower attachment to meat consumption. Furthermore, injunctive norms in favor of plant-based meals were linked with higher support for measures promoting plant-based meals in schools, in both samples (parents, teachers). Lower meat attachment and favorable perceived meal attributes (e.g., perceptions about plant-based and fish meals) were associated with teachers' support for measures promoting plant-based meals in schools. These findings suggest that future efforts and research with parents and teachers to enable less meat-centric and more flexitarian food practices in schools should consider social and motivation variables relevant to plant-forward transitions.


Assuntos
Docentes , Instituições Acadêmicas , Humanos , Dieta , Refeições , Política Nutricional , Pais
4.
Appetite ; 187: 106597, 2023 08 01.
Artigo em Inglês | MEDLINE | ID: mdl-37178929

RESUMO

Collective meal contexts such as restaurants, cafeterias and canteens can help accelerate transitions to healthier and more sustainable diets. However, evidence from intervention studies on these contexts lacks integration. This scoping review aimed to map determinants of dietary change in collective meal contexts across multiple settings, interventions, target groups, and target behaviors. The review provided two main outcomes: (i) identifying intervention components to promote dietary change in collective meal contexts, based on the existing body of evidence; and (ii) classifying and integrating these intervention components into an overarching framework of behavior change (i.e., COM-B system). The review encompassed twenty-eight databases via two indexing services and extracted information from 232 primary sources (27,458 records selected for title and abstract screening, 574 articles selected for full-text screening). We identified a total of 653 intervention activities, which were classified into intervention components and grouped under three broad themes, namely contextual and environmental changes, social influence, and knowledge and behavioral regulation. Multi-component interventions tended to report overall positive outcomes. The review proposes several directions for future research, including: (i) moving toward more theory-based interventions in collective meal contexts; (ii) providing more detailed information about intervention settings, implementation, target groups, activities, and materials; and (iii) improving the use of open science practices in the field. Furthermore, the review offers a free, original, open-access list and synthesis of 277 intervention studies in collective meal contexts, which can help intervention planners and evaluators optimize their efforts to promote healthier and more sustainable food practices in these contexts.


Assuntos
Dieta , Refeições , Humanos
5.
J Am Soc Nephrol ; 33(7): 1323-1340, 2022 07.
Artigo em Inglês | MEDLINE | ID: mdl-35581010

RESUMO

BACKGROUND: Impaired mineral ion metabolism is a hallmark of CKD-metabolic bone disorder. It can lead to pathologic vascular calcification and is associated with an increased risk of cardiovascular mortality. Loss of calcium-sensing receptor (CaSR) expression in vascular smooth muscle cells exacerbates vascular calcification in vitro. Conversely, vascular calcification can be reduced by calcimimetics, which function as allosteric activators of CaSR. METHODS: To determine the role of the CaSR in vascular calcification, we characterized mice with targeted Casr gene knockout in vascular smooth muscle cells ( SM22α CaSR Δflox/Δflox ). RESULTS: Vascular smooth muscle cells cultured from the knockout (KO) mice calcified more readily than those from control (wild-type) mice in vitro. However, mice did not show ectopic calcifications in vivo but they did display a profound mineral ion imbalance. Specifically, KO mice exhibited hypercalcemia, hypercalciuria, hyperphosphaturia, and osteopenia, with elevated circulating fibroblast growth factor 23 (FGF23), calcitriol (1,25-D3), and parathyroid hormone levels. Renal tubular α-Klotho protein expression was increased in KO mice but vascular α-Klotho protein expression was not. Altered CaSR expression in the kidney or the parathyroid glands could not account for the observed phenotype of the KO mice. CONCLUSIONS: These results suggest that, in addition to CaSR's established role in the parathyroid-kidney-bone axis, expression of CaSR in vascular smooth muscle cells directly contributes to total body mineral ion homeostasis.


Assuntos
Receptores de Detecção de Cálcio , Calcificação Vascular , Animais , Cálcio/metabolismo , Modelos Animais de Doenças , Fatores de Crescimento de Fibroblastos/metabolismo , Proteínas Klotho , Camundongos , Camundongos Knockout , Minerais/metabolismo , Músculo Liso Vascular/metabolismo , Miócitos de Músculo Liso/metabolismo , Receptores de Detecção de Cálcio/genética , Receptores de Detecção de Cálcio/metabolismo , Calcificação Vascular/etiologia
6.
Appetite ; 170: 105875, 2022 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-34932989

RESUMO

Consumers in modern society are often less exposed to meat that resembles the animal, and thus are less familiar with it, making it difficult to disentangle the influence of these two inputs (familiarity vs. animal resemblance) on meat appetite. Across three studies, we sought to systematically disentangle the impact of familiarity and animal resemblance on meat appetite using inductive (Study 1) and experimental (Studies 2a-2b) approaches. In Study 1 (N = 229) we separated familiarity and animal resemblance into orthogonal dimensions using 28 meat products. Participants provided free associations and rated the products on familiarity, animal resemblance, and appetitive appeal. In Studies 2a and 2b (N = 514) we experimentally examined the independent contributions of familiarity and animal resemblance, using stimuli normed in Study 1. We hypothesized that animal resemblance has its most pronounced influence on appetite when meat products are unfamiliar. Participants' free associations and ratings of the products were in line with this conditional hypothesis (Study1), as were the experimental manipulations of familiarity and animal resemblance (Studies 2a-2b), confirmed by a mini meta-analysis. In all three studies, familiarity had a pervasive influence on appetite. These findings suggest that product familiarity can attenuate the psychological impact that animal reminders have on appetite. Thus, interventions aimed at eliciting animal associations with meat should consider the familiarity of the products employed.


Assuntos
Apetite , Produtos da Carne , Animais , Humanos , Carne/análise , Reconhecimento Psicológico
7.
Appetite ; 156: 104860, 2021 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-32916208

RESUMO

Understanding consumer perceptions of meat alternatives is key to facilitating a shift toward more sustainable food consumption. Importantly, these perceptions may vary according to the characteristics of the consumer (e.g., preferences, motivations), the product (e.g., sensory attributes) and the encounter (e.g., how the meat alternative is presented/framed). Qualitative and quantitative methods were applied to examine consumer perceptions of five proposed alternatives to meat: legumes, tofu, seitan, lab-grown meat, and insects. In Study 1, 138 participants provided free associations with regards to conventional animal proteins (e.g., red/white meat, fish) and the five alternatives. Three profiles of consumers were identified: (1) hedonically motivated meat eaters uninterested in meat substitutes; (2) health-oriented meat eaters open to some meat substitutes; and (3) ethically conscious meat avoiders positively oriented to most meat alternatives. In Study 2, the presentation of the product was experimentally manipulated: 285 participants evaluated the same five meat alternatives along several dimensions (e.g., edibility, healthiness), either when framed as an individual product or as part of a larger meal. Overall, most meat alternatives benefited from a meal framing, with the notable exception of legumes, which benefited from an individual framing, and insects which were evaluated quite negatively regardless of framing. The present findings suggest that there is not a single way to frame all meat alternatives that will improve their appeal to all consumers.


Assuntos
Comportamento do Consumidor , Preferências Alimentares , Animais , Carne , Percepção , Verduras
8.
J Child Sex Abus ; 29(7): 802-820, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32835633

RESUMO

Child sexual abuse myths legitimize abusive behaviors, involving high levels of victim blame and low levels of offender liability. The present study aims to: (i) adapt a measure of endorsement of child sexual abuse myths to the Portuguese context (i.e., Child Sexual Abuse Myth Scale - CSAMS); and (ii) provide validity and reliability evidence for this measure. A total of 423 adults (66.2% female) filled out a sociodemographic questionnaire, the Ambivalent Sexism Inventory, and the CSAMS. The CSAMS validity and reliability results supported the original structure, which comprises three dimensions: Blame Diffusion (e.g., 'Adolescent girls who wear very revealing clothing are asking to be sexually abused'), Restrictive Stereotypes (e.g., 'Most children are sexually abused by strangers or by men who are not well known to the child'), and Denial of Abusiveness (e.g., 'Older children, who have a better understanding of sexual matters, have a responsibility to actively resist sexual advances by adults'). Configural and metric invariance by sex were held, and criterion validity was observed through significant associations between myths, sexism and sex. This study provided evidence in support of the validity and reliability of the Portuguese version of the Child Sexual Abuse Myth Scale.


Assuntos
Abuso Sexual na Infância/estatística & dados numéricos , Proteção da Criança/estatística & dados numéricos , Percepção Social , Estereotipagem , Inquéritos e Questionários/normas , Adulto , Criança , Abuso Sexual na Infância/diagnóstico , Mecanismos de Defesa , Feminino , Humanos , Portugal , Psicometria , Reprodutibilidade dos Testes , Sobreviventes/estatística & dados numéricos
9.
Appetite ; 140: 19-26, 2019 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-31059762

RESUMO

There have been increasing calls for triggering and sustaining a large-scale transition toward healthier and more sustainable food systems. To help materialize this transition, the present work aims to inform efforts for developing, marketing and promoting plant-based meals and plant-forward lifestyles, following a consumption-focused approach. The findings (Nparticipants = 1600, Portugal; 52.6% female, Mage = 48.30) allowed to identify trends and differences on three sets of variables - (a) current eating habits (i.e., meat, fish, and plant-based meals), (b) consumer willingness to change (i.e., reduce meat consumption, follow a plant-based diet, maintain the status quo), and (c) enablers for eating plant-based meals more often (i.e., capability, opportunity, motivation) -, considering consumer orientations toward consumption in general, and food consumption in particular. Taken together, the results suggested that some consumption orientations were aligned with the transition to more plant-based diets (e.g., food orientation toward naturalness), others were open to - but not yet materialized in - the transition (e.g., general orientation toward consumption as exploration), and still others were in tension with the transition (e.g., food orientation toward pleasure). The discussion calls for developing and testing pathways to reduce meat consumption and increase plant-based eating which capture and build upon a range of consumption orientations, rather than against them.


Assuntos
Dieta Vegetariana/psicologia , Dieta/psicologia , Comportamento Alimentar/psicologia , Abastecimento de Alimentos , Motivação , Adulto , Comportamento de Escolha , Conservação dos Recursos Naturais , Dieta/métodos , Feminino , Humanos , Masculino , Marketing , Pessoa de Meia-Idade , Portugal
10.
Psychol Health Med ; 21(5): 562-70, 2016 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-26683266

RESUMO

INTRODUCTION: The aims of the present study were to assess demographic and clinical characteristics of patients after receiving a cancer diagnosis, and to determine possible risk factors for anxiety and depression. METHODS: All consecutive patients aged 18 or above, were assessed before starting intravenous chemotherapy for the first time with the Hospital Anxiety and Depression Scale (HADS), the Distress Thermometer, and a Visual Analog Scale for pain. Demographic and clinical data were also collected. RESULTS: The patients assessed (n = 270) had a mean age of 59.4 (SD = 11.8) years, and 50.7% were women. Tumours were more frequently colorectal (27.2%), lung (18.8%) and breast (17.6%), and 68.9% were stages 3 or 4. A HADS Anxiety score ≥8 was present in 30% of the patients, a Depression score ≥8 in 24.1%, and a Distress score ≥4 in 44.4%. Independent risk factors for HADS Depression score ≥8 were being a woman (OR = 2.45; p = 0.004), being older (OR = 1.04; p = 0.005), and cancer stage 3-4 (OR = 2.24; p = 0.023) in the multivariable analysis; for Anxiety ≥8 they were being a woman (OR = 2.47; p = 0.002), having a past psychiatric consultation (OR = 2.83; p = 0.029), and cancer stage 3-4 (OR = 1.90; p = 0.047). CONCLUSION: These results suggest the need for greater awareness and a differentiated approach to patients at increased risk of anxiety and depression in the early stages of treatment and before starting chemotherapy.


Assuntos
Ansiedade/psicologia , Depressão/psicologia , Neoplasias/psicologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Ansiedade/epidemiologia , Neoplasias da Mama/patologia , Neoplasias da Mama/psicologia , Dor do Câncer/epidemiologia , Dor do Câncer/psicologia , Neoplasias Colorretais/patologia , Neoplasias Colorretais/psicologia , Estudos Transversais , Depressão/epidemiologia , Feminino , Humanos , Modelos Logísticos , Neoplasias Pulmonares/patologia , Neoplasias Pulmonares/psicologia , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Estadiamento de Neoplasias , Neoplasias/patologia , Fatores de Risco , Fatores Sexuais , Estresse Psicológico/psicologia , Adulto Jovem
11.
Appetite ; 95: 113-25, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26148456

RESUMO

In response to calls to expand knowledge on consumer willingness to reduce meat consumption and to adopt a more plant-based diet, this work advances the construct of meat attachment and the Meat Attachment Questionnaire (MAQ). The MAQ is a new measure referring to a positive bond towards meat consumption. It was developed and validated through three sequential studies following from an in-depth approach to consumer representations of meat. The construct and initial pool of items were firstly developed drawing on qualitative data from 410 participants in a previous work on consumers' valuation of meat. Afterwards, 1023 participants completed these items and other measures, providing data to assess item selection, factor structure, reliability, convergent and concurrent validity, and predictive ability. Finally, a sample of 318 participants from a different cultural background completed the final version of the MAQ along with other measures to assess measurement invariance, reliability and predictive ability. Across samples, a four-factor solution (i.e., hedonism, affinity, entitlement, and dependence) with 16 items and a second-order global dimension of meat attachment fully met criteria for good model fit. The MAQ subscales and global scale were associated with attitudes towards meat, subjective norm, human supremacy beliefs, eating habits, and dietary identity. They also provided additional explanatory variance above and beyond the core TPB variables (i.e. attitudes, subjective norm and perceived behavioral control) in willingness and intentions concerning meat substitution. Overall, the findings point towards the relevance of the MAQ for the study of meat consumption and meat substitution, and lend support to the idea that holding a pattern of attachment towards meat may hinder a shift towards a more plant-based diet.


Assuntos
Dieta/psicologia , Comportamento Alimentar/psicologia , Intenção , Carne , Inquéritos e Questionários , Adulto , Animais , Cultura , Feminino , Preferências Alimentares/psicologia , Conhecimentos, Atitudes e Prática em Saúde , Humanos , Masculino , Reprodutibilidade dos Testes , Adulto Jovem
12.
Appetite ; 90: 80-90, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25747854

RESUMO

A shift towards reduced meat consumption and a more plant-based diet is endorsed to promote sustainability, improve public health, and minimize animal suffering. However, large segments of consumers do not seem willing to make such transition. While it may take a profound societal change to achieve significant progresses on this regard, there have been limited attempts to understand the psychosocial processes that may hinder or facilitate this shift. This study provides an in-depth exploration of how consumer representations of meat, the impact of meat, and rationales for changing or not habits relate with willingness to adopt a more plant-based diet. Multiple Correspondence Analysis was employed to examine participant responses (N = 410) to a set of open-ended questions, free word association tasks and closed questions. Three clusters with two hallmarks each were identified: (1) a pattern of disgust towards meat coupled with moral internalization; (2) a pattern of low affective connection towards meat and willingness to change habits; and (3) a pattern of attachment to meat and unwillingness to change habits. The findings raise two main propositions. The first is that an affective connection towards meat relates to the perception of the impacts of meat and to willingness to change consumption habits. The second proposition is that a set of rationales resembling moral disengagement mechanisms (e.g., pro-meat justifications; self-exonerations) arise when some consumers contemplate the consequences of meat production and consumption, and the possibility of changing habits.


Assuntos
Afeto , Comportamento de Escolha , Comportamento do Consumidor/estatística & dados numéricos , Dieta/psicologia , Comportamento Alimentar/psicologia , Preferências Alimentares/psicologia , Adulto , Feminino , Conhecimentos, Atitudes e Prática em Saúde , Humanos , Masculino , Carne , Pessoa de Meia-Idade , Inquéritos e Questionários , Verduras , Adulto Jovem
13.
Sci Rep ; 14(1): 13033, 2024 06 13.
Artigo em Inglês | MEDLINE | ID: mdl-38871837

RESUMO

Men tend to eat more meat than women, but it is not clear why. We tested three hypotheses in a cross-cultural design (20,802 individuals in 23 countries across four continents): that gender differences are (a) universal, (b) related to gender roles and thus weaker in countries with higher gender equality and human development, or (c) related to opportunities to express gender roles and thus stronger in countries with higher gender equality and human development. Across all countries, men tended to consume more meat than women. However, this difference increased significantly in countries with greater human development and gender equality. The paradoxical gender gap in meat consumption aligns with previous research that suggests greater differences in behavior across genders in contexts that are more developed and gender equal. We discuss implications for theories of culture and gender as well as practical implications for global meat reduction.


Assuntos
Carne , Feminino , Masculino , Humanos , Fatores Sexuais , Comparação Transcultural , Adulto , Comportamento Alimentar , Cultura , Papel de Gênero , Pessoa de Meia-Idade , Adulto Jovem
14.
Arq Bras Cir Dig ; 36: e1766, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37851752

RESUMO

BACKGROUND: In the surgical correction of large incisional hernias, the use of a prosthesis is essential in most cases regardless of the technique chosen. The preference is for the polypropylene prosthesis. AIMS: To compare the onlay and Rives-Stoppa techniques in the correction of incisional hernias, their immediate results, complications, advantages, and disadvantages. METHODS: Two groups of patients with incisional hernias were analyzed, submitted to the onlay (19 patients) and Rives-Stoppa (17 patients) techniques, and that used polypropylene prostheses. General epidemiological variables, perioperative data variables, and postoperative complications were assessed. RESULTS: The patients' epidemiologic profile was similar between both groups. The majority were women (58.4%), with a mean age of 65.5 years and a previous mean body mass index of 41.5 kg/m². The Rives-Stoppa technique was employed in most patients (52.7%). Those submitted to the onlay technique had longer abdominal drainage time and longer hospital stay, as well as a higher incidence of seromas and surgical wound infection. CONCLUSIONS: The incisional herniorrhaphy technique with the placement of a pre-peritoneal polypropylene mesh by the Rives-Stoppa technique was superior to the onlay due to lower rates of drain use, hospital stay, and postoperative complications.


Assuntos
Hérnia Ventral , Hérnia Incisional , Humanos , Masculino , Feminino , Idoso , Hérnia Incisional/cirurgia , Polipropilenos , Resultado do Tratamento , Telas Cirúrgicas/efeitos adversos , Recidiva , Hérnia Ventral/cirurgia , Herniorrafia/métodos , Complicações Pós-Operatórias/epidemiologia
15.
Child Maltreat ; 27(3): 356-365, 2022 08.
Artigo em Inglês | MEDLINE | ID: mdl-33467901

RESUMO

Research on attitudes toward Child Sexual Abuse (CSA) consistently shows that men are more likely to endorse myths about CSA events, victims and perpetrators, compared to women. Here we present two studies that examine why these gender differences occur. Study one (N = 439) followed a dispositional approach to test the mediating role of empathy, social dominance orientation (SDO) and propensity for moral disengagement in the association between gender and the endorsement of CSA myths. Male participants showed higher levels of SDO and propensity for moral disengagement, and lower empathy, which in turn were associated with greater CSA myths acceptance. Study two (N = 360) followed a situational approach to test these processes using a specific case of CSA. Male participants showed higher levels of SDO and lower empathy, which in turn were associated with lower scores of perceived assault seriousness, victim credibility, perpetrator culpability, and greater victim culpability. Overall, the results suggest that men and women may appraise CSA differently, which can be partly explained by differences in SDO, propensity to morally disengage, and empathy. Furthermore, different cognitive mechanisms may be activated with regard to general appraisals of CSA compared to specific cases of CSA.


Assuntos
Abuso Sexual na Infância , Vítimas de Crime , Criança , Abuso Sexual na Infância/psicologia , Vítimas de Crime/psicologia , Feminino , Humanos , Masculino , Fatores Sexuais
16.
Food Microbiol ; 27(3): 339-46, 2010 May.
Artigo em Inglês | MEDLINE | ID: mdl-20227598

RESUMO

The microbiological profile in raw milk cheeses is typically characterized by a multitude of microbial groups, with interactions among them throughout ripening that are not fully understood to date. Incidence of undesired microorganisms in raw cheesemaking milk, as is the case of either spoilage or even pathogenic ones, is a common trait in Portuguese traditional cheeses. Hence, they will likely contribute to the physicochemical changes occurring therein and, consequently, to the characteristics of the final product. In order to gain insight into their role, model cheese systems, manufactured as far as possible according to artisanal practices (except that the initial microbial load and biodiversity were controlled), were experimentally tested. Single contaminants, or a consortium thereof, were inoculated at two levels in sterilized raw ewe's milk, and duly combined with inocula containing one or two lactic acid bacteria normally found in those traditional cheeses. The physicochemical composition, organic acid profile, and evolution of both protein breakdown and rheology were monitored throughout a 60 d-ripening period. Modifications brought about within the cheese matrix as a result of microbial metabolism, especially those arising from the interaction between lactic acid bacteria and unwanted microorganisms, included the enhanced release of peptides and free amino acids, which in turn led to higher viscoelastic moduli. The final model cheeses could be well discriminated, based on the impact of the various inocula considered upon the levels of organic acids. Conversely, proteolysis and viscoelastic properties appeared to be essentially independent of the initial microflora.


Assuntos
Queijo/microbiologia , Queijo/normas , Manipulação de Alimentos/métodos , Microbiologia de Alimentos , Lactobacillaceae/crescimento & desenvolvimento , Animais , Contagem de Colônia Microbiana , Qualidade de Produtos para o Consumidor , Escherichia coli/crescimento & desenvolvimento , Fermentação , Humanos , Lactobacillaceae/fisiologia , Listeria/crescimento & desenvolvimento , Leite/microbiologia , Dinâmica Populacional , Portugal , Pseudomonas aeruginosa/crescimento & desenvolvimento , Reologia , Ovinos , Staphylococcus aureus/crescimento & desenvolvimento
17.
Waste Manag ; 118: 552-562, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32980734

RESUMO

The increasing concern with greenhouse gas emissions and nutrients cycling creates a need for cost-effective, practical and environmentally sensible biowaste management strategies. Centralized systems have struggled to comply with those needs. Decentralized systems, treating waste at source, promise local nutrient circularity and increased resource sovereignty. The large-scale performance of decentralized systems remains unclear, especially concerning the local sink capacity to assimilate the treatment products. This study aimed to compare centralized and decentralized systems for the region of Porto and assess whether creating additional urban farms could reduce costs and environmental impacts. Spatial analysis was used to assess waste generation, potential compost bin locations, peri-urban and potential urban farmland available, and collection and transport requirements. The carbon footprint of different scenarios was determined using life-cycle assessment. The results show that local composting led to cost savings over centralized systems. However, this system encompassed positive carbon emissions and most districts evidenced limited sink capacity for compost application. Additional urban farms added significant sink capacity, however, their impact on cost and carbon footprint was insignificant. The carbon footprint of centralized systems was heavily dependent on factors influencing collection such as population density, and affected by the renewable content of the electricity grid. Anaerobic digestion was the most climate-friendly option in the urban center and local composting in remote and less dense districts. Municipalities may benefit from tailoring the treatment systems to specific districts, creating additional jobs while reducing cost and climate impacts overall.


Assuntos
Compostagem , Gases de Efeito Estufa , Pegada de Carbono , Cidades , Fazendas
18.
J Food Prot ; 72(11): 2243-51, 2009 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-19903385

RESUMO

An experiment using model ewe's milk cheeses was designed to characterize microbial interactions that arise in actual raw milk cheese environments. These model cheeses were manufactured according to Portuguese artisanal practices, except that the microbial load and biodiversity were fully controlled: single potential pathogens and spoilage bacteria, or a combination thereof, were combined at various initial inoculum levels in sterilized raw ewe's milk with several lactic acid bacteria (LAB) normally found in traditional cheeses. Viable microbial counts were monitored throughout a 60-day ripening period. Two alternative mathematical approaches were used to fit the experimental data generated in terms of population dynamics: percent of inhibition and D-values. These were able to explain the complex competitive interactions between the contaminant microorganisms and the LAB adventitious populations. In general, the tested LAB were less able to inhibit contaminants present in combination and in higher concentrations. Lactococcus lactis, with its strong acidifying potential, was the most effective factor in controlling the unwanted bacterial population, especially single Staphylococcus aureus. The two lactobacilli studied, especially Lactobacillus brevis, were shown to be less effective; Escherichia coli and Listeria innocua were the contaminants least inhibited by the LAB.


Assuntos
Antibiose , Queijo/microbiologia , Qualidade de Produtos para o Consumidor , Lactobacillaceae/fisiologia , Animais , Queijo/normas , Contagem de Colônia Microbiana , Escherichia coli/crescimento & desenvolvimento , Fermentação , Microbiologia de Alimentos , Humanos , Cinética , Listeria/crescimento & desenvolvimento , Dinâmica Populacional , Ovinos , Staphylococcus aureus/crescimento & desenvolvimento
19.
Animals (Basel) ; 9(8)2019 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-31344828

RESUMO

There has been increasing interest in the study of human-animal relations. This contrasts with the lack of normative resources and materials for research purposes. We present subjective norms for a set of 120 open-source colour images of animals spanning a total of 12 biological categories (e.g., mammals, insects, reptiles, arachnids). Participants (N = 509, 55.2% female, MAge = 28.05, SD = 9.84) were asked to evaluate a randomly selected sub-set of 12 animals on valence, arousal, familiarity, cuteness, dangerousness, edibility, similarity to humans, capacity to think, capacity to feel, acceptability to kill for human consumption and feelings of care and protection. Animal evaluations were affected by individual characteristics of the perceiver, particularly gender, diet and companion animal ownership. Moral attitudes towards animals were predominantly predicted by ratings of cuteness, edibility, capacity to feel and familiarity. The Animal Images Database (Animal.ID) is the largest open-source database of rated images of animals; the stimuli set and item-level data are freely available online.

20.
Med Phys ; 46(8): 3615-3626, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31087394

RESUMO

PURPOSE: The presence of range uncertainties hinders the exploitation of the full potential of charged particle therapy. Several range verification techniques have been proposed to mitigate this limitation. Prompt gamma spectroscopy (PGS) is among the most promising solutions for online and in vivo range verification. In this work, we present the experimental results of the detection of prompt gamma radiation, induced by 4 He beams at the Heidelberg Ion-Beam Therapy Center (HIT). The results were obtained, using a spectroscopic unit of which the design has been optimized using Monte Carlo simulations. METHODS: The spectroscopic unit is composed by a primary cerium bromide (CeBr 3 ) crystal surrounded by a secondary bismuth germanate (BGO) crystal for anticoincidence detection (AC). The digitalization of the signals is performed with an advanced FADC/FPGA system. The 4 He beams at clinical energies and intensities are delivered to multiple targets in the experimental cave at the HIT. We analyze the production of prompt gamma on oxygen and carbon targets, as well as high Z materials such as titanium and aluminum. The quantitative analysis includes a systematic comparison of the signal-to-noise ratio (SNR) improvement for the spectral lines when introducing the AC detection. Moreover, the SNR improvement could provide a reduction of the number of events required to draw robust conclusions. We perform a statistic analysis to determine the magnitude of such an effect. RESULTS: We present the energy spectra detected by the primary CeBr 3 and the secondary BGO. The combination of these two detectors leads to an average increase of the signal-to-noise ratio by a factor 2.1, which confirms the Monte Carlo predictions. The spectroscopic unit is capable of detecting efficiently the discrete gamma emission over the full energy spectrum. We identify and analyze 19 independent spectral lines in an energy range spacing from E γ = 0.718  MeV to E γ = 6.13  MeV. Moreover, when introducing the AC detection, the number of events required to determine robustly the intensity of the discrete lines decreases. Finally, the analysis of the low-energy reaction lines determines whether a thin metal insert is introduced in the beam direction. CONCLUSIONS: This work provides the experimental characterization of the spectroscopy unit in development for range verification through PGS at the HIT. Excellent performances have been demonstrated over the full prompt gamma energy spectrum with 4 He beams at clinical energies and intensities.


Assuntos
Cério/química , Raios gama , Hélio/uso terapêutico , Contagem de Cintilação/instrumentação , Análise Espectral
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