Effects of leaf and stem maturation on nutritional value in Megathyrsus maximus.

BACKGROUND: Megathyrsus maximus is a forage grass native to Africa but widely cultivated in tropical and subtropical regions of the world where it is part of the grazing food chain. This study aimed to evaluate five M. maximus genotypes for the effect of maturity on their morpho-agronomic traits, nutritional composition and digestibility, and to correlate their leaf blade and stem anatomy with their nutritional value. RESULTS: The proportion of sclerenchyma tissues increased as maturity was reached, while lignin accumulation was differentiated between genotypes. Gatton Panic, Green Panic and Mutale genotypes maintained their acid detergent lignin (ADL) values for leaf blades in the three cuts evaluated. In sacco ruminal dry matter disappearance was lower in Green Panic genotype at the vegetative stage for stems, but not for leaf blades. Significant positive correlations were found between dry matter disappearance and mesophyll tissues, and the latter were negatively correlated with neutral detergent fiber (NDF) and ADL. CONCLUSION: Our results strongly indicate that cutting age and genotype affected the nutritional value of M. maximus leaf blades and stems, with a more pronounced loss of quality in stems than in leaf blades. We recommend increasing the frequency of grazing at early stage or anticipating the stage of stem elongation in Green Panic to produce forage with better nutritional value. © 2023 Society of Chemical Industry.

Contrasting adaptive responses to cope with drought stress and recovery in Cenchrus ciliaris L. and their implications for tissue lignification.

Cenchrus ciliaris L. is a widely used species for cattle feed in arid and semi-arid regions due to good forage value and known tolerance to drought conditions. Here, we provide insights to adaptive responses of two contrasting genotypes of C. ciliaris (drought-tolerant "RN51" and drought-sensitive "RN1") to face drought stress and recovery conditions and the implications for tissue lignification. Drought stress caused a reversible decrease in the leaf water relationship and damage to photosystem II, leading to an increased generation of reactive oxygen species and lipid peroxidation. Plants of RN51 exhibited a pronounced increase of antioxidant enzymatic activities. Unlike the drought-sensitive genotype, RN51 exhibited further development of lignified tissues and bulliform cells and had the greatest thickness of the adaxial epidermis. Drought stress led to the rapid activation of the expression of lignin biosynthesis pathway-related enzymes. The transcript level of the caffeoyl-CoA O-methyltransferase gene decreased in RN1, whereas cinnamoyl-CoA reductase transcripts were increased in RN51. After rewatering, the tolerant genotype recovered more rapidly than RN1. Even though the two genotypes survived when they were exposed to drought stress, RN1 showed the highest reduction in growth parameters, and this reduction was sustained during rewatering. The results indicated that the capacity to regulate lipid peroxidation and mitigate oxidative damage could be one of the mechanisms included in tolerance to drought stress. In addition, the development of foliar characteristics, like thickness of the adaxial epidermis, well-developed bulliform cells, and intensive lignified tissues, are considered anatomical adaptive strategies for drought tolerance in C. ciliaris.

Genotypic variation in response to salinity in a new sexual germplasm of Cenchrus ciliaris L.

As part of a breeding program for new salt-tolerant sexual genotypes of Cenchrus ciliaris L., here we evaluated the salt-stress response of two new sexual hybrids, obtained by controlled crosses, at seedling and germination stages. A seedling hydroponic experiment with 300 mM NaCl was performed and physiological variables and growth components were evaluated. While salt-treated sexual material did not show a decrease in productivity with respect to control plants, a differential response in some physiological characteristics was observed. Sexual hybrid 1-9-1 did not suffer oxidative damage and its proline content did not differ from that of control treatment. By contrast, sexual hybrid 1-7-11 suffered oxidative damage and accumulated proline, maintaining its growth under saline stress. At the germination stage, sexual hybrid 1-9-1 presented the highest Germination Rate Index at the maximum NaCl concentration assayed, suggesting an ecological advantage in this genotype. These new sexual resources are promising maternal parental with differential response to salt and could be incorporated in a breeding program of C. ciliaris in the search of new genotypes tolerant to salinity.

Reactive oxygen species in the elongation zone of maize leaves are necessary for leaf extension.

The production and role of reactive oxygen species (ROS) in the expanding zone of maize (Zea mays) leaf blades were investigated. ROS release along the leaf blade was evaluated by embedding intact seedlings in 2',7'-dichlorofluorescein-containing agar and examining the distribution of 2',7'-dichlorofluorescein fluorescence along leaf 4, which was exposed by removing the outer leaves before embedding the seedling. Fluorescence was high in the expanding region, becoming practically non-detectable beyond 65 mm from the ligule, indicating high ROS production in the expansion zone. Segments obtained from the elongation zone of leaf 4 were used to assess the role of ROS in leaf elongation. The distribution of cerium perhydroxide deposits in electron micrographs indicated hydrogen peroxide (H(2)O(2)) presence in the apoplast. 2',7'-Dichlorofluorescein fluorescence and apoplastic H(2)O(2) accumulation were inhibited with diphenyleneiodonium (DPI), which also inhibited O*(2)(-) generation, suggesting a flavin-containing enzyme activity such as NADPH oxidase was involved in ROS production. Segments from the elongation zone incubated in water grew 8% in 2 h. KI treatments, which scavenged H(2)O(2) but did not inhibit O*(2)(-) production, did not modify growth. DPI significantly inhibited segment elongation, and the addition of H(2)O(2) (50 or 500 microM) to the incubation medium partially reverted the inhibition caused by DPI. These results indicate that a certain concentration of H(2)O(2) is necessary for leaf elongation, but it could not be distinguished whether H(2)O(2), or other ROS, are the actual active agents.

Cytochemical variations of human von Ebner's glands

Se analizaron las glándulas de von Ebner humanas durante el desarrollo fetal, infantil y adulto, con el propósito de observar posibles cambios citoquímicos en relación con la edad. Se utilizaron lenguas de fetos de 8 a 38 semanas, muestras de la V lingual de niños recién nacidos, de 8 a 14 años, y adultos de 20 años en adelante, empleándose H/E, y técnicas para muco sustancias (PAS, PAS/amilasa, PAS/sialidasa, metanamina/plata, Alcian blue a pH 2,5 y 1,0 Azul de toluidina a pH 3,8). Entre las 16 y 20 semanas se identificaron acinosa y conductos en formación, estando el parénquima completamente desarrollado a las 24 semanas. Las glándulas de recién nacidos presentaban morfológicamente el aspecto de acinos serosos basófilos y periodatonegativos. En el niño las células secretoras contenían gránulos apicales PAS positivos sensibles a la sialidasa, periodatorreactivos y ligeramente metacromáticos. Estas características se acrecentaron con la edad. En el adulto el citoplasma apical de los adenómeros y el contenido luminal eran además alcianófilos. Las variaciones citoquímicas observadas indican que las glándulas de von Ebner humanas están constituidas por células de tipo seromucoso que contienen sialomucinas y sulfomucinas