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Ai Zheng ; 21(9): 957-60, 2002 Sep.
Artigo em Zh | MEDLINE | ID: mdl-12508541

RESUMO

BACKGROUND & OBJECTIVE: The aim of this study was to express and purify human endostatin and to prepare polyclonal antibody of mouse anti-human endostatin. METHODS: The cDNA of endostatin was amplified by PCR, then recombined into prokaryotic expression vector and transformed into Escherichia coli BL21 for expression; the mice were immunized with purified products. RESULTS: Prokaryotic expression vector pQE-30 of human endostatin was successfully constructed; the expression product was gained after pQE-30 was transferred into BL21. After purified by Ni affinity chromatography, the product was identified to be a single component by SDS-PAGE. Western blot analysis showed that high titer mouse anti-human endostatin polyclonal antibody was successfully prepared. CONCLUSION: Highly purified expression product and prepared polyclonal antibody provide the necessary material for further study.


Assuntos
Anticorpos Monoclonais/imunologia , Colágeno/genética , Fragmentos de Peptídeos/genética , Animais , Western Blotting , Cromatografia de Afinidade , Colágeno/imunologia , Endostatinas , Escherichia coli/genética , Expressão Gênica , Humanos , Camundongos , Fragmentos de Peptídeos/imunologia , Proteínas Recombinantes/genética , Proteínas Recombinantes/imunologia
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