Manure Microbial Communities and Resistance Profiles Reconfigure after Transition to Manure Pits and Differ from Those in Fertilized Field Soil.

In agricultural settings, microbes and antimicrobial resistance genes (ARGs) have the potential to be transferred across diverse environments and ecosystems. The consequences of these microbial transfers are unclear and understudied. On dairy farms, the storage of cow manure in manure pits and subsequent application to field soil as a fertilizer may facilitate the spread of the mammalian gut microbiome and its associated ARGs to the environment. To determine the extent of both taxonomic and resistance similarity during these transitions, we collected fresh manure, manure from pits, and field soil across 15 different dairy farms for three consecutive seasons. We used a combination of shotgun metagenomic sequencing and functional metagenomics to quantitatively interrogate taxonomic and ARG compositional variation on farms. We found that as the microbiome transitions from fresh dairy cow manure to manure pits, microbial taxonomic compositions and resistance profiles experience distinct restructuring, including decreases in alpha diversity and shifts in specific ARG abundances that potentially correspond to fresh manure going from a gut-structured community to an environment-structured community. Further, we did not find evidence of shared microbial community or a transfer of ARGs between manure and field soil microbiomes. Our results suggest that fresh manure experiences a compositional change in manure pits during storage and that the storage of manure in manure pits does not result in a depletion of ARGs. We did not find evidence of taxonomic or ARG restructuring of soil microbiota with the application of manure to field soils, as soil communities remained resilient to manure-induced perturbation.IMPORTANCE The addition of dairy cow manure-stored in manure pits-to field soil has the potential to introduce not only organic nutrients but also mammalian microbial communities and antimicrobial resistance genes (ARGs) to soil communities. Using shotgun sequencing paired with functional metagenomics, we showed that microbial community composition changed between fresh manure and manure pit samples with a decrease in gut-associated pathobionts, while ARG abundance and diversity remained high. However, field soil communities were distinct from those in manure in both microbial taxonomic and ARG composition. These results broaden our understanding of the transfer of microbial communities in agricultural settings and suggest that field soil microbial communities are resilient against the deposition of ARGs or microbial communities from manure.

Psychosocial work characteristics predict cardiovascular disease risk factors and health functioning in rural women: the Wisconsin Rural Women's Health Study.

BACKGROUND: The aim of the present study is to investigate the association between psychosocial work characteristics and health functioning and cardiovascular disease risk factors among rural women of central Wisconsin and compare psychosocial work characteristics between farm and nonfarm women. METHODS: Stratified sampling was used to select a random sample (n = 1500) of farm and nonfarm women aged 25 to 71 years from the Central Marshfield Epidemiologic Study Area. The baseline examination included measurements of blood pressure, height, weight, and fasting blood lipids, glucose, and insulin. Psychosocial job condition was measured with the Karasek Job Content Questionnaires (JCQ). Health functioning was assessed by the Short Form-36 Health Survey. RESULTS: The analysis of JCQ showed that nonfarm residents were more likely to have jobs with high demand and high decision latitude compared to farm residents. Also, the farm residents (40.3%) were more likely to be occupied in passive jobs (jobs with low levels of demand and control) than the nonfarm residents (26.9%). Among farm residents, psychological job demand was associated with HDL level (beta = 0.17), triglycerides (beta = 0.0), their ratio (beta = 0.005), and blood insulin level (beta = 0.014), and among nonfarm residents, psychological job demand was associated with diastolic blood pressure (beta = 0.17) and total cholesterol level (beta = 0.002). CONCLUSION: Our results showed that rural farm residents had a higher prevalence of CVD risk factors and were more likely to be occupied in jobs with low levels of demand and control. Job stress predicted more CVD risk factors among farm residents compared to nonfarm residents. Therefore, interventions reducing job strain among rural farm residents are timely and necessary.

Vacations improve mental health among rural women: the Wisconsin Rural Women's Health Study.

OBJECTIVE: To compare psychological stress, quality of marital life, and disruptive homelife due to work among rural women of central Wisconsin who take vacations frequently and those who do not. METHODS: Women were recruited from 1996 to 2001 for a prospective cohort study from the Marshfield Epidemiologic Study area, a geographic area in central Wisconsin. Stratified sampling was used to select a random sample of 1500 farm and non-farm resident women. RESULTS: The odds of depression and tension were higher among women who took vacations only once in 2 years (Depression: OR=1.92, 95% CI=1.2,3.0; Tension: OR=1.7, 95%CI=1.2, 2.3) or once in 6 years (Depression: OR=1.97, 95% CI=1.2, 3.2; Tension: OR=1.9, 95% CI=1.3,2.8) compared to women who took vacations twice or more per year. The odds of marital satisfaction decreased as the frequency of vacations decreased. CONCLUSION: Women who take vacations frequently are less likely to become tense, depressed, or tired, and are more satisfied with their marriage. These personal psychological benefits that lead to increased quality of life may also lead to improved work performance.

A comparison of cardiovascular disease risk factors in farm and non-farm residents: the Wisconsin Rural Women's Health Study.

OBJECTIVES: The purpose of this paper is to describe the baseline difference in cardiovascular disease risk factors between farm and non-farm women in the Rural Women's Health Study. METHODS: Women aged 25 to 75 years were recruited from the Central Marshfield Epidemiologic Study Area, a geographically defined area surrounding Marshfield, Wis. (population 19,000), where more than 95% of residents seek their health care from the Marshfield Clinic. The baseline examination included measurements of blood pressure, skin folds, height, weight, fasting blood lipids, and blood glucose. Health behavior information collected included smoking, dietary intake, reproductive health, physical activity, medical history, social support, occupational strain and symptoms of anxiety and depression. RESULTS: Between 1995 and 2001, 825 non-farm residents and 675 farm residents were examined (58% of eligible women). The farm residents were similar in age to the non-farm residents (mean 47.6 years and 47.0 years, t-test = -0.91, P value = 0.36). The total number of modifiable risk factors for atherosclerosis ranged theoretically from 0 to 6, and actually from 0 to 5, with a small percentage of women having either none or 5 risk factors. The percentage of women with 3 or more modifiable risk factors was 26.1% (95% CL = 23.9, 28.4). The median number of total risk factors was 2 and the frequency distribution was not significantly different between farm and non-farm residents (chi 2 5 df = 4.6, P = 0.47). The prevalence of current cigarette smoking was significantly higher in the non-farm residents, while the prevalence of hypertension and obesity was significantly higher in the farm residents. Overall, obesity prevalence is significantly higher in the study cohort than US women in general (35% versus 23%). Only 5 (0.7%) of the farm residents and 10 (1.2%) of the non-farm residents reported a previous myocardial infarction. CONCLUSIONS: These data suggest that the prevalence of risk factors for atherosclerosis, and hence future coronary heart disease, is very high in rural women. Obesity prevention through increased physical activity would be appropriate for both farm and non-farm residents, while quit smoking campaigns should be specifically targeted towards non-farm resident women.

Differences in preventive screening rates in Wisconsin farm and non-farm resident women.

PURPOSE: To determine the proportion of rural women who met screening recommendations for Pap smears, mammograms, blood cholesterol, and blood pressure measurements. METHODS: Women aged 25-75 were recruited for a population-based study of chronic diseases in rural residents. In addition to a self-administered health questionnaire and a brief examination, the most recent Pap smear, mammogram, blood pressure, and blood cholesterol measurements were documented from electronic medical records. RESULTS: The study population was comprised of 675 farm and 825 non-farm residents. Fifty-five women (3.7%) had no documentation in their medical records of having ever had a Pap smear, and 19.3% (95% CL 16.9, 21.7) of women aged 40 and older had no documentation of a mammogram. Ninety-two percent of the women (95% CL = 90.2, 93.7) had a blood pressure measurement in the previous 2 years and 74.9% (95% CL = 72.2, 77.6) had a blood cholesterol measurement in the previous 5 years. Age was inversely related to Pap smears and positively correlated with mammograms, blood pressure, and blood cholesterol measurements. Farm residents were significantly less likely to have Pap smears or blood pressure measurements at recommended time intervals. DISCUSSION: To increase the proportion of rural women in compliance with preventive screening recommendations, public health education efforts should target farm residents and younger women.

Safety and health in biomass production, transportation, and storage: a commentary based on the biomass and biofuels session at the 2013 North American Agricultural Safety Summit.

There is significant interest in biomass production ranging from government agencies to the private sector, both inside and outside of the traditional production agricultural setting. This interest has led to an increase in the development and production of biomass crops. Much of this effort has focused on specific segments of the process, and more specifically on the mechanics of these individual segments. From a review of scientific literature, it is seen that little effort has been put into identifying, classifying and preventing safety hazards in on-farm biomass production systems. This commentary describes the current status of the knowledge pertaining to health and safety factors of biomass production and storage in the US and identifies areas of standards development that the biomass industry needs from the agricultural safety and health community.

Biofuels and North American agriculture--implications for the health and safety of North American producers.

This decade has provided North American agricultural producers with opportunity to not only produce fiber and food, but also fuel and other industrial products. The drivers incenting this development could be sustained well into the future, therefore workforce safety and health implications are likely to persist for some time. Within production agriculture, the 'feedstock growth and harvest cycle' and 'transport' sectors possess the changing exposures experienced by workers. The Conference explored the following exposures: distiller's grains and bio-processing byproducts, spent catalyst, solvent brine, microbial agents, genetically modified organisms, discharge effluent, H2O dilutes, change in cropping patterns and resultant use of different seeding and harvest technologies, pests (whether target or non-target), and rural traffic resulting from concentrated movement of massive quantities of biomass and grain. Other issues of a more general public health nature such as watershed implications, other environmental impacts, emissions, uneven economic development potential, public safety issues associated with transport of both fuel and other industrial products, and rural emergency medical service need were explored. And, agronomic impacts were noted, including tillage change, potassium buildup in soil, nutrient depletion, sedimentation and erosion of tillable soil, and local esthetics. It was concluded that rural venues for formation and exploration of public policy need to be created.

Wisconsin rural women's health study psychological factors and blood cholesterol level: difference between normal and overweight rural women.

OBJECTIVE: The aim of the present study is to investigate the effects of psychological factors on plasma lipid levels among rural women of central Wisconsin and to compare the effects of these variables among normal-weight and overweight women. METHODS: Stratified sampling was used to select a random sample (n=1500) of farm and non-farm women aged 25 to 71 years from the Central Marshfield Epidemiologic Study Area. The baseline examination included measurements of blood pressure, skin folds, height, weight, and fasting blood lipids, glucose, and insulin. Framingham study questionnaires were employed to measure anger, anxiety, tension, and marital disagreement. The Spielberger Trait anger-reaction subscale was employed to assess proneness to anger. RESULTS: Among normal-weight women, a positive association was found between anger-reaction scores and cholesterol (b=0.008), ratio (b=0.014), triglycerides (b=0.02), and LDL (b=0.07). The odds of elevated cholesterol were highest among women with high scores on the Speilberger anger-reaction scale (OR=2.0) and anger discussion scale (OR=2.0), while the odds were less among women with high scores on the anger-out scale (OR=0.59). However, among overweight women, we found only scores on the Framingham anger-discussion scale as an important factor to determine the plasma lipid levels. CONCLUSION: Anger management may help to sever the link between psychological factors and CHD risk factors. Intervention intended to prevent cardiac events through the reduction of stress and modification of related psychological risk factors have successfully improved the CHD risk factors profile. Similar studies are needed to determine the efficacy of intervention for the primary prevention of CHD risk factors.

Issues of agricultural safety and health.

Agricultural work is the most prevalent type of employment in the world. In the United States only a few are engaged in creating food and fiber for many. Agriculture includes farming, ranching, fishing, and forestry, and together they carry significant risk for the development of injury or illness. There are numerous special-population issues related to agriculture. Farmers are old and growing older, many workers are children, and migrant and seasonal help, often foreign born, make up a large percentage of the workforce. It has been only relatively recently that concerns of agricultural safety and health have become a major research focus in the United States.

Assessment of personal protective equipment use among Midwestern farmers.

BACKGROUND: Personal protective equipment (PPE) is readily available and widely recommended, yet usage among agricultural workers is largely unknown. METHODS: A mail survey of 2,483 farmers in six Midwestern states with telephone follow-up addressed PPE usage related to sun exposure, noise abatement, chain saw usage, welding and metal work, handling of large animals in and out of confinement facilities, feed handling, manure storage facilities, and mixing and applying chemicals. Summary statistics of survey responses were compiled. RESULTS: With the exception of welding masks, PPE usage was low. Farmers were satisfied with availability of PPE through local hardware and farm cooperatives, but the decision to use PPE was personal and influenced little by outside parties. CONCLUSION: PPE usage rates by farmers on Midwestern farms need to be increased. The desire of the individual farmer to reduce risk of personal injury or exposure should be targeted. The most effective venue will be local agricultural extension services.