RESUMO
In this study, we present a method for assembling biofunctionalized paper into a multiform structured scaffold system for reliable tissue regeneration using an origami-based approach. The surface of a paper was conformally modified with a poly(styrene-co-maleic anhydride) layer via initiated chemical vapor deposition followed by the immobilization of poly-l-lysine (PLL) and deposition of Ca(2+). This procedure ensures the formation of alginate hydrogel on the paper due to Ca(2+) diffusion. Furthermore, strong adhesion of the alginate hydrogel on the paper onto the paper substrate was achieved due to an electrostatic interaction between the alginate and PLL. The developed scaffold system was versatile and allowed area-selective cell seeding. Also, the hydrogel-laden paper could be folded freely into 3D tissue-like structures using a simple origami-based method. The cylindrically constructed paper scaffold system with chondrocytes was applied into a three-ring defect trachea in rabbits. The transplanted engineered tissues replaced the native trachea without stenosis after 4 wks. As for the custom-built scaffold system, the hydrogel-laden paper system will provide a robust and facile method for the formation of tissues mimicking native tissue constructs.
Assuntos
Hidrogel de Polietilenoglicol-Dimetacrilato/farmacologia , Papel , Engenharia Tecidual/métodos , Alicerces Teciduais/química , Alginatos/química , Animais , Cartilagem/efeitos dos fármacos , Cartilagem/fisiologia , Condrócitos/citologia , Condrócitos/efeitos dos fármacos , Condrócitos/transplante , Força Compressiva , Ácido Glucurônico/química , Células HeLa , Ácidos Hexurônicos/química , Humanos , Maleatos/química , Camundongos Endogâmicos BALB C , Camundongos Nus , Microscopia Eletrônica de Varredura , Peso Molecular , Neovascularização Fisiológica/efeitos dos fármacos , Poliestirenos/química , Coelhos , Espectrometria por Raios X , Traqueia/efeitos dos fármacos , Traqueia/fisiologiaRESUMO
In this study, we investigated various highly porous extracellular matrix (ECM)-based cryogels for cartilage tissue engineering. For the fabrication of ECM-based cryogels, either methacrylated chondroitin sulfate (MeCS) or methacrylated hyaluronic acid (MeHA) were cross-linked along with poly (ethylene glycol) diacrylates (PEGDA) via free radical polymerization under freezing conditions. This procedure induces ice crystallization (used as a porogen) prior polymer crosslinking in which, after complete cryopolymerization, a thawing process transforms the ice crystals into a unique interconnected macroporous structure within ECM-cryogels. The developed ECM-cryogels exhibited an average macroporosity of 75% and supported the infiltration of chondrocyteds. When rabbit chondrocytes were cultured on ECM-cryogels, MeCS-based cryogels stimulated aggrecan gene expression and GAG accumulation, whereas MeHA-based cryogels stimulated type II collagen gene expression and collagen accumulation. These results demonstrate that design of ECM-based cryogels can play an important role in promoting specific ECM proteins secretion for cartilage tissue engineering.