Ensemble modelling, uncertainty and robust predictions of organic carbon in long-term bare-fallow soils.

Simulation models represent soil organic carbon (SOC) dynamics in global carbon (C) cycle scenarios to support climate-change studies. It is imperative to increase confidence in long-term predictions of SOC dynamics by reducing the uncertainty in model estimates. We evaluated SOC simulated from an ensemble of 26 process-based C models by comparing simulations to experimental data from seven long-term bare-fallow (vegetation-free) plots at six sites: Denmark (two sites), France, Russia, Sweden and the United Kingdom. The decay of SOC in these plots has been monitored for decades since the last inputs of plant material, providing the opportunity to test decomposition without the continuous input of new organic material. The models were run independently over multi-year simulation periods (from 28 to 80 years) in a blind test with no calibration (Bln) and with the following three calibration scenarios, each providing different levels of information and/or allowing different levels of model fitting: (a) calibrating decomposition parameters separately at each experimental site (Spe); (b) using a generic, knowledge-based, parameterization applicable in the Central European region (Gen); and (c) using a combination of both (a) and (b) strategies (Mix). We addressed uncertainties from different modelling approaches with or without spin-up initialization of SOC. Changes in the multi-model median (MMM) of SOC were used as descriptors of the ensemble performance. On average across sites, Gen proved adequate in describing changes in SOC, with MMM equal to average SOC (and standard deviation) of 39.2 (±15.5) Mg C/ha compared to the observed mean of 36.0 (±19.7) Mg C/ha (last observed year), indicating sufficiently reliable SOC estimates. Moving to Mix (37.5 ± 16.7 Mg C/ha) and Spe (36.8 ± 19.8 Mg C/ha) provided only marginal gains in accuracy, but modellers would need to apply more knowledge and a greater calibration effort than in Gen, thereby limiting the wider applicability of models.

Fixed choice design and augmented fixed choice design for network data with missing observations.

The statistical analysis of social networks is increasingly used to understand social processes and patterns. The association between social relationships and individual behaviors is of particular interest to sociologists, psychologists, and public health researchers. Several recent network studies make use of the fixed choice design (FCD), which induces missing edges in the network data. Because of the complex dependence structure inherent in networks, missing data can pose very difficult problems for valid statistical inference. In this article, we introduce novel methods for accounting for the FCD censoring and introduce a new survey design, which we call the augmented fixed choice design (AFCD). The AFCD adds considerable information to analyses without unduly burdening the survey respondent, resulting in improvements over the FCD, and other existing estimators. We demonstrate this new method through simulation studies and an analysis of alcohol use in a network of undergraduate students living in a residence hall.

The Discriminative Kalman Filter for Bayesian Filtering with Nonlinear and Nongaussian Observation Models.

The Kalman filter provides a simple and efficient algorithm to compute the posterior distribution for state-space models where both the latent state and measurement models are linear and gaussian. Extensions to the Kalman filter, including the extended and unscented Kalman filters, incorporate linearizations for models where the observation model p(observation|state) is nonlinear. We argue that in many cases, a model for p(state|observation) proves both easier to learn and more accurate for latent state estimation. Approximating p(state|observation) as gaussian leads to a new filtering algorithm, the discriminative Kalman filter (DKF), which can perform well even when p(observation|state) is highly nonlinear and/or nongaussian. The approximation, motivated by the Bernstein-von Mises theorem, improves as the dimensionality of the observations increases. The DKF has computational complexity similar to the Kalman filter, allowing it in some cases to perform much faster than particle filters with similar precision, while better accounting for nonlinear and nongaussian observation models than Kalman-based extensions. When the observation model must be learned from training data prior to filtering, off-the-shelf nonlinear and nonparametric regression techniques can provide a gaussian model for p(observation|state) that cleanly integrates with the DKF. As part of the BrainGate2 clinical trial, we successfully implemented gaussian process regression with the DKF framework in a brain-computer interface to provide real-time, closed-loop cursor control to a person with a complete spinal cord injury. In this letter, we explore the theory underlying the DKF, exhibit some illustrative examples, and outline potential extensions.

Assessing uncertainties in crop and pasture ensemble model simulations of productivity and N2 O emissions.

Simulation models are extensively used to predict agricultural productivity and greenhouse gas emissions. However, the uncertainties of (reduced) model ensemble simulations have not been assessed systematically for variables affecting food security and climate change mitigation, within multi-species agricultural contexts. We report an international model comparison and benchmarking exercise, showing the potential of multi-model ensembles to predict productivity and nitrous oxide (N2 O) emissions for wheat, maize, rice and temperate grasslands. Using a multi-stage modelling protocol, from blind simulations (stage 1) to partial (stages 2-4) and full calibration (stage 5), 24 process-based biogeochemical models were assessed individually or as an ensemble against long-term experimental data from four temperate grassland and five arable crop rotation sites spanning four continents. Comparisons were performed by reference to the experimental uncertainties of observed yields and N2 O emissions. Results showed that across sites and crop/grassland types, 23%-40% of the uncalibrated individual models were within two standard deviations (SD) of observed yields, while 42 (rice) to 96% (grasslands) of the models were within 1 SD of observed N2 O emissions. At stage 1, ensembles formed by the three lowest prediction model errors predicted both yields and N2 O emissions within experimental uncertainties for 44% and 33% of the crop and grassland growth cycles, respectively. Partial model calibration (stages 2-4) markedly reduced prediction errors of the full model ensemble E-median for crop grain yields (from 36% at stage 1 down to 4% on average) and grassland productivity (from 44% to 27%) and to a lesser and more variable extent for N2 O emissions. Yield-scaled N2 O emissions (N2 O emissions divided by crop yields) were ranked accurately by three-model ensembles across crop species and field sites. The potential of using process-based model ensembles to predict jointly productivity and N2 O emissions at field scale is discussed.

Robust Closed-Loop Control of a Cursor in a Person with Tetraplegia using Gaussian Process Regression.

Intracortical brain computer interfaces can enable individuals with paralysis to control external devices through voluntarily modulated brain activity. Decoding quality has been previously shown to degrade with signal nonstationarities-specifically, the changes in the statistics of the data between training and testing data sets. This includes changes to the neural tuning profiles and baseline shifts in firing rates of recorded neurons, as well as nonphysiological noise. While progress has been made toward providing long-term user control via decoder recalibration, relatively little work has been dedicated to making the decoding algorithm more resilient to signal nonstationarities. Here, we describe how principled kernel selection with gaussian process regression can be used within a Bayesian filtering framework to mitigate the effects of commonly encountered nonstationarities. Given a supervised training set of (neural features, intention to move in a direction)-pairs, we use gaussian process regression to predict the intention given the neural data. We apply kernel embedding for each neural feature with the standard radial basis function. The multiple kernels are then summed together across each neural dimension, which allows the kernel to effectively ignore large differences that occur only in a single feature. The summed kernel is used for real-time predictions of the posterior mean and variance under a gaussian process framework. The predictions are then filtered using the discriminative Kalman filter to produce an estimate of the neural intention given the history of neural data. We refer to the multiple kernel approach combined with the discriminative Kalman filter as the MK-DKF. We found that the MK-DKF decoder was more resilient to nonstationarities frequently encountered in-real world settings yet provided similar performance to the currently used Kalman decoder. These results demonstrate a method by which neural decoding can be made more resistant to nonstationarities.

Ambiguity and nonidentifiability in the statistical analysis of neural codes.

Many experimental studies of neural coding rely on a statistical interpretation of the theoretical notion of the rate at which a neuron fires spikes. For example, neuroscientists often ask, "Does a population of neurons exhibit more synchronous spiking than one would expect from the covariability of their instantaneous firing rates?" For another example, "How much of a neuron's observed spiking variability is caused by the variability of its instantaneous firing rate, and how much is caused by spike timing variability?" However, a neuron's theoretical firing rate is not necessarily well-defined. Consequently, neuroscientific questions involving the theoretical firing rate do not have a meaning in isolation but can only be interpreted in light of additional statistical modeling choices. Ignoring this ambiguity can lead to inconsistent reasoning or wayward conclusions. We illustrate these issues with examples drawn from the neural-coding literature.

Neuronal ensemble synchrony during human focal seizures.

Seizures are classically characterized as the expression of hypersynchronous neural activity, yet the true degree of synchrony in neuronal spiking (action potentials) during human seizures remains a fundamental question. We quantified the temporal precision of spike synchrony in ensembles of neocortical neurons during seizures in people with pharmacologically intractable epilepsy. Two seizure types were analyzed: those characterized by sustained gamma (∼40-60 Hz) local field potential (LFP) oscillations or by spike-wave complexes (SWCs; ∼3 Hz). Fine (<10 ms) temporal synchrony was rarely present during gamma-band seizures, where neuronal spiking remained highly irregular and asynchronous. In SWC seizures, phase locking of neuronal spiking to the SWC spike phase induced synchrony at a coarse 50-100 ms level. In addition, transient fine synchrony occurred primarily during the initial ∼20 ms period of the SWC spike phase and varied across subjects and seizures. Sporadic coherence events between neuronal population spike counts and LFPs were observed during SWC seizures in high (∼80 Hz) gamma-band and during high-frequency oscillations (∼130 Hz). Maximum entropy models of the joint neuronal spiking probability, constrained only on single neurons' nonstationary coarse spiking rates and local network activation, explained most of the fine synchrony in both seizure types. Our findings indicate that fine neuronal ensemble synchrony occurs mostly during SWC, not gamma-band, seizures, and primarily during the initial phase of SWC spikes. Furthermore, these fine synchrony events result mostly from transient increases in overall neuronal network spiking rates, rather than changes in precise spiking correlations between specific pairs of neurons.

Spatiotemporal conditional inference and hypothesis tests for neural ensemble spiking precision.

The collective dynamics of neural ensembles create complex spike patterns with many spatial and temporal scales. Understanding the statistical structure of these patterns can help resolve fundamental questions about neural computation and neural dynamics. Spatiotemporal conditional inference (STCI) is introduced here as a semiparametric statistical framework for investigating the nature of precise spiking patterns from collections of neurons that is robust to arbitrarily complex and nonstationary coarse spiking dynamics. The main idea is to focus statistical modeling and inference not on the full distribution of the data, but rather on families of conditional distributions of precise spiking given different types of coarse spiking. The framework is then used to develop families of hypothesis tests for probing the spatiotemporal precision of spiking patterns. Relationships among different conditional distributions are used to improve multiple hypothesis-testing adjustments and design novel Monte Carlo spike resampling algorithms. Of special note are algorithms that can locally jitter spike times while still preserving the instantaneous peristimulus time histogram or the instantaneous total spike count from a group of recorded neurons. The framework can also be used to test whether first-order maximum entropy models with possibly random and time-varying parameters can account for observed patterns of spiking. STCI provides a detailed example of the generic principle of conditional inference, which may be applicable to other areas of neurostatistical analysis.

Characterizing drought stress and trait influence on maize yield under current and future conditions.

Global climate change is predicted to increase temperatures, alter geographical patterns of rainfall and increase the frequency of extreme climatic events. Such changes are likely to alter the timing and magnitude of drought stresses experienced by crops. This study used new developments in the classification of crop water stress to first characterize the typology and frequency of drought-stress patterns experienced by European maize crops and their associated distributions of grain yield, and second determine the influence of the breeding traits anthesis-silking synchrony, maturity and kernel number on yield in different drought-stress scenarios, under current and future climates. Under historical conditions, a low-stress scenario occurred most frequently (ca. 40%), and three other stress types exposing crops to late-season stresses each occurred in ca. 20% of cases. A key revelation shown was that the four patterns will also be the most dominant stress patterns under 2050 conditions. Future frequencies of low drought stress were reduced by ca. 15%, and those of severe water deficit during grain filling increased from 18% to 25%. Despite this, effects of elevated CO2 on crop growth moderated detrimental effects of climate change on yield. Increasing anthesis-silking synchrony had the greatest effect on yield in low drought-stress seasonal patterns, whereas earlier maturity had the greatest effect in crops exposed to severe early-terminal drought stress. Segregating drought-stress patterns into key groups allowed greater insight into the effects of trait perturbation on crop yield under different weather conditions. We demonstrate that for crops exposed to the same drought-stress pattern, trait perturbation under current climates will have a similar impact on yield as that expected in future, even though the frequencies of severe drought stress will increase in future. These results have important ramifications for breeding of maize and have implications for studies examining genetic and physiological crop responses to environmental stresses.

Measuring instability in chronic human intracortical neural recordings towards stable, long-term brain-computer interfaces.

Intracortical brain-computer interfaces (iBCIs) enable people with tetraplegia to gain intuitive cursor control from movement intentions. To translate to practical use, iBCIs should provide reliable performance for extended periods of time. However, performance begins to degrade as the relationship between kinematic intention and recorded neural activity shifts compared to when the decoder was initially trained. In addition to developing decoders to better handle long-term instability, identifying when to recalibrate will also optimize performance. We propose a method to measure instability in neural data without needing to label user intentions. Longitudinal data were analyzed from two BrainGate2 participants with tetraplegia as they used fixed decoders to control a computer cursor spanning 142 days and 28 days, respectively. We demonstrate a measure of instability that correlates with changes in closed-loop cursor performance solely based on the recorded neural activity (Pearson r = 0.93 and 0.72, respectively). This result suggests a strategy to infer online iBCI performance from neural data alone and to determine when recalibration should take place for practical long-term use.

Accelerated spike resampling for accurate multiple testing controls.

Controlling for multiple hypothesis tests using standard spike resampling techniques often requires prohibitive amounts of computation. Importance sampling techniques can be used to accelerate the computation. The general theory is presented, along with specific examples for testing differences across conditions using permutation tests and for testing pairwise synchrony and precise lagged-correlation between many simultaneously recorded spike trains using interval jitter.

Optimizing Nitrogen Fertilization to Enhance Productivity and Profitability of Upland Rice Using CSM-CERES-Rice.

Nitrogen (N) deficiency can limit rice productivity, whereas the over- and underapplication of N results in agronomic and economic losses. Process-based crop models are useful tools and could assist in optimizing N management, enhancing the production efficiency and profitability of upland rice production systems. The study evaluated the ability of CSM-CERES-Rice to determine optimal N fertilization rate for different sowing dates of upland rice. Field experimental data from two growing seasons (2018-2019 and 2019-2020) were used to simulate rice responses to four N fertilization rates (N30, N60, N90 and a control-N0) applied under three different sowing windows (SD1, SD2 and SD3). Cultivar coefficients were calibrated with data from N90 under all sowing windows in both seasons and the remaining treatments were used for model validation. Following model validation, simulations were extended up to N240 to identify the sowing date's specific economic optimum N fertilization rate (EONFR). Results indicated that CSM-CERES-Rice performed well both in calibration and validation, in simulating rice performance under different N fertilization rates. The d-index and nRMSE values for grain yield (0.90 and 16%), aboveground dry matter (0.93 and 13%), harvest index (0.86 and 7%), grain N contents (0.95 and 18%), total crop N uptake (0.97 and 15%) and N use efficiencies (0.94-0.97 and 11-15%) during model validation indicated good agreement between simulated and observed data. Extended simulations indicated that upland rice yield was responsive to N fertilization up to 180 kg N ha-1 (N180), where the yield plateau was observed. Fertilization rates of 140, 170 and 130 kg N ha-1 were identified as the EONFR for SD1, SD2 and SD3, respectively, based on the computed profitability, marginal net returns and N utilization. The model results suggested that N fertilization rate should be adjusted for different sowing windows rather than recommending a uniform N rate across sowing windows. In summary, CSM-CERES-Rice can be used as a decision support tool for determining EONFR for seasonal sowing windows to maximize the productivity and profitability of upland rice production.

Prefrontal network engagement by deep brain stimulation in limbic hubs.

Prefrontal circuits in the human brain play an important role in cognitive and affective processing. Neuromodulation therapies delivered to certain key hubs within these circuits are being used with increasing frequency to treat a host of neuropsychiatric disorders. However, the detailed neurophysiological effects of stimulation to these hubs are largely unknown. Here, we performed intracranial recordings across prefrontal networks while delivering electrical stimulation to two well-established white matter hubs involved in cognitive regulation and depression: the subcallosal cingulate (SCC) and ventral capsule/ventral striatum (VC/VS). We demonstrate a shared frontotemporal circuit consisting of the ventromedial prefrontal cortex, amygdala, and lateral orbitofrontal cortex where gamma oscillations are differentially modulated by stimulation target. Additionally, we found participant-specific responses to stimulation in the dorsal anterior cingulate cortex and demonstrate the capacity for further tuning of neural activity using current-steered stimulation. Our findings indicate a potential neurophysiological mechanism for the dissociable therapeutic effects seen across the SCC and VC/VS targets for psychiatric neuromodulation and our results lay the groundwork for personalized, network-guided neurostimulation therapy.

Conditional modeling and the jitter method of spike resampling.

The existence and role of fine-temporal structure in the spiking activity of central neurons is the subject of an enduring debate among physiologists. To a large extent, the problem is a statistical one: what inferences can be drawn from neurons monitored in the absence of full control over their presynaptic environments? In principle, properly crafted resampling methods can still produce statistically correct hypothesis tests. We focus on the approach to resampling known as jitter. We review a wide range of jitter techniques, illustrated by both simulation experiments and selected analyses of spike data from motor cortical neurons. We rely on an intuitive and rigorous statistical framework known as conditional modeling to reveal otherwise hidden assumptions and to support precise conclusions. Among other applications, we review statistical tests for exploring any proposed limit on the rate of change of spiking probabilities, exact tests for the significance of repeated fine-temporal patterns of spikes, and the construction of acceptance bands for testing any purported relationship between sensory or motor variables and synchrony or other fine-temporal events.

Behavior-dependent short-term assembly dynamics in the medial prefrontal cortex.

Although short-term plasticity is believed to play a fundamental role in cortical computation, empirical evidence bearing on its role during behavior is scarce. Here we looked for the signature of short-term plasticity in the fine-timescale spiking relationships of a simultaneously recorded population of physiologically identified pyramidal cells and interneurons, in the medial prefrontal cortex of the rat, in a working memory task. On broader timescales, sequentially organized and transiently active neurons reliably differentiated between different trajectories of the rat in the maze. On finer timescales, putative monosynaptic interactions reflected short-term plasticity in their dynamic and predictable modulation across various aspects of the task, beyond a statistical accounting for the effect of the neurons' co-varying firing rates. Seeking potential mechanisms for such effects, we found evidence for both firing pattern-dependent facilitation and depression, as well as for a supralinear effect of presynaptic coincidence on the firing of postsynaptic targets.

Evaluating the Impact of Nitrogen Application on Growth and Productivity of Maize Under Control Conditions.

Climatic conditions significantly affect the maize productivity. Among abiotic factors, nitrogen (N) fertilizer and temperature are the two important factors which dominantly affect the maize (Zea mays L.) production during the early crop growth stages. Two experiments were conducted to determine the impact of N fertilizer and temperature on the maize growth and yield. In the first experiment, the maize hybrids were screened for their sensitivity to temperature variations. The screening was based on the growth performance of the hybrids under three temperatures (T 1 = ambient open-air temperature, T 2 = 1°C higher than the ambient temperature, and T 3 = 1°C lower than the ambient temperature) range. The results showed that an increase in temperature was resulted less 50% emergence and mean emergence (4.1 and 6.3 days, respectively), while emergence energy and full emergence were higher (25.4 and 75.2%, respectively) under the higher temperature exposure. The results showed that Syngenta 7720 and Muqabla S 25W87 were temperature tolerant and sensitive maize hybrids, respectively. The second experiment was carried out to study the response of the two selected maize hybrids (Syngenta 7720 and Muqabla S 25W87) to four N fertilizer applications. The results revealed that the maximum N use efficiency (19.5 kg kg-1) was achieved in maize hybrids with low N application (75 kg N ha-1 equivalent to 1.13 g N plant-1). However, the maximum maize grain yield (86.4 g plant-1), dry weight (203 g plant-1), and grain protein content (15.0%) were observed in maize hybrids that were grown with the application of 300 kg N ha-1 (equivalent to 4.52 g N plant-1). Therefore, it is recommended that the application of 300 kg N ha-1 to temperature tolerant maize hybrid may be considered best agricultural management practices for obtaining optimum maize grain yield under present changing climate.

PELP: Accounting for Missing Data in Neural Time Series by Periodic Estimation of Lost Packets.

Recent advances in wireless data transmission technology have the potential to revolutionize clinical neuroscience. Today sensing-capable electrical stimulators, known as "bidirectional devices", are used to acquire chronic brain activity from humans in natural environments. However, with wireless transmission come potential failures in data transmission, and not all available devices correctly account for missing data or provide precise timing for when data losses occur. Our inability to precisely reconstruct time-domain neural signals makes it difficult to apply subsequent neural signal processing techniques and analyses. Here, our goal was to accurately reconstruct time-domain neural signals impacted by data loss during wireless transmission. Towards this end, we developed a method termed Periodic Estimation of Lost Packets (PELP). PELP leverages the highly periodic nature of stimulation artifacts to precisely determine when data losses occur. Using simulated stimulation waveforms added to human EEG data, we show that PELP is robust to a range of stimulation waveforms and noise characteristics. Then, we applied PELP to local field potential (LFP) recordings collected using an implantable, bidirectional DBS platform operating at various telemetry bandwidths. By effectively accounting for the timing of missing data, PELP enables the analysis of neural time series data collected via wireless transmission-a prerequisite for better understanding the brain-behavior relationships underlying neurological and psychiatric disorders.

Periodic Artifact Removal With Applications to Deep Brain Stimulation.

Deep brain stimulation (DBS) therapies have shown clinical success in the treatment of a number of neurological illnesses, including obsessive-compulsive disorder, epilepsy, and Parkinson's disease. An emerging strategy for increasing the efficacy of DBS therapies is to develop closed-loop, adaptive DBS systems that can sense biomarkers associated with particular symptoms and in response, adjust DBS parameters in real-time. The development of such systems requires extensive analysis of the underlying neural signals while DBS is on, so that candidate biomarkers can be identified and the effects of varying the DBS parameters can be better understood. However, DBS creates high amplitude, high frequency stimulation artifacts that prevent the underlying neural signals and thus the biological mechanisms underlying DBS from being analyzed. Additionally, DBS devices often require low sampling rates, which alias the artifact frequency, and rely on wireless data transmission methods that can create signal recordings with missing data of unknown length. Thus, traditional artifact removal methods cannot be applied to this setting. We present a novel periodic artifact removal algorithm for DBS applications that can accurately remove stimulation artifacts in the presence of missing data and in some cases where the stimulation frequency exceeds the Nyquist frequency. The numerical examples suggest that, if implemented on dedicated hardware, this algorithm has the potential to be used in embedded closed-loop DBS therapies to remove DBS stimulation artifacts and hence, to aid in the discovery of candidate biomarkers in real-time. Code for our proposed algorithm is publicly available on Github.

Does face-selective cortex show a left visual field bias for centrally-viewed faces?

The left half of a centrally-viewed face contributes more strongly to recognition performance than the right. This left visual field (LVF) advantage is typically attributed to an untested assumption that face-selective cortex in the right hemisphere (RH) exhibits a contralateral bias, even for centrally-viewed faces. We tested the validity of this assumption using a behavioral measure of the LVF advantage and an fMRI experiment that measured laterality of face-selective cortex and neural contralateral bias. In the behavioral experiment, participants performed a chimeric face-matching task (Harrison and Strother, 2019). In the fMRI experiment, participants viewed chimeric faces comprised of face halves that either repeated or changed simultaneously in both hemifields, or repeated in one hemifield and changed in the other. This enabled us to measure lateralization of fMRI face-repetition suppression and hemifield-specific half-face sensitivity in face-selective cortex. We found that LVF bias in the fusiform face area (FFA) and right-lateralization of the FFA for changing versus repeated faces were both positively correlated with a behavioral measure of the LVF advantage for upright (but not inverted) faces. Results from regression analyses showed that LVF bias in the right FFA and FFA laterality make separable contributions to the prediction of our behavioral measure of the LVF bias for upright faces. Our results confirm a ubiquitous but previously untested assumption that RH superiority combined with contralateral bias in face-selective cortex explains the LVF advantage in face recognition. Specifically, our results show that neural LVF bias in the right FFA is sufficient to explain the relationship between FFA laterality and the perceptual LVF bias for centrally-viewed faces.

How do packet losses affect measures of averaged neural signalsƒ.

Recent advances in implanted device development have enabled chronic streaming of neural data to external devices allowing for long timescale, naturalistic recordings. However, characteristic data losses occur during wireless transmission. Estimates for the duration of these losses are typically uncertain reducing signal quality and impeding analyses. To characterize the effect of these losses on recovery of averaged neural signals, we simulated neural time series data for a typical event-related potential (ERP) experiment. We investigated how the signal duration and the degree of timing uncertainty affected the offset of the ERP, its duration in time, its amplitude, and the ability to resolve small differences corresponding to different task conditions. Simulations showed that long timescale signals were generally robust to the effects of packet losses apart from timing offsets while short timescale signals were significantly delocalized and attenuated. These results provide clarity on the types of signals that can be resolved using these datasets and provide clarity on the restrictions imposed by data losses on typical analyses.