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Plant Physiol Biochem ; 46(1): 34-45, 2008 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-18061466

RESUMO

A drought screen identified accessions of Solanum tuberosum ssp. andigena that showed varying degrees of physiological acclimation or adaptation to repeated drought stress. The accessions also showed variable tuber phenotypes from small tubers that failed to develop in an accession that showed photosynthetic adaptation to normal tubers in an accession with a phenotype showing some degree of photosynthetic adaptation and acclimation. Using microarray data, we correlated the expression of genes associated with carbon metabolism with the tuber development phenotypes under drought. Genes associated with sucrose and starch metabolism showed responses consistent with starch deficiency in the adapted accession and normal starch deposition in the intermediate accession. Starch phosphorylase and glycogen bound starch synthase were induced in the adapted accession, which had abnormal tuber development. Genes associated with trehalose were induced in the intermediate accession with normal tuber development. Genes associated with respiration were also induced in the intermediate accession, and a pattern compatible with the existence of a 3PGA recovery pathway was revealed. Expression of thioredoxin genes also correlated with tuber development phenotypes under drought stress. The data suggest differential regulation of starch deposition in accessions of Andigena with different abilities to respond to drought stress.


Assuntos
Aclimatação/fisiologia , Carbono/metabolismo , Regulação da Expressão Gênica de Plantas/fisiologia , Genes de Plantas/fisiologia , Tubérculos/fisiologia , Solanum tuberosum/fisiologia , Metabolismo dos Carboidratos/fisiologia , Desastres , Perfilação da Expressão Gênica , Análise de Sequência com Séries de Oligonucleotídeos , Consumo de Oxigênio/fisiologia , Fotossíntese/fisiologia , Amido/biossíntese , Amido/genética , Tiorredoxinas/biossíntese , Tiorredoxinas/genética , Trealose/biossíntese , Trealose/genética
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