RESUMO
Astrocytes throughout the central nervous system are heterogeneous in both structure and function. This diversity leads to tissue-specific specialization where morphology is adapted to the surrounding neuronal circuitry, as seen in Bergman glia of the cerebellum and Müller glia of the retina. Because morphology can be a differentiating factor for cellular classification, we recently developed a mouse where glial-fibrillary acidic protein (GFAP)-expressing cells stochastically label for full membranous morphology. Here we utilize this tool to investigate whether morphological and electrophysiological features separate types of mouse retinal astrocytes. In this work, we report on a novel glial population found in the inner plexiform layer and ganglion cell layer which expresses the canonical astrocyte markers GFAP, S100ß, connexin-43, Sox2 and Sox9. Apart from their retinal layer localization, these cells are unique in their radial distribution. They are notably absent from the mid-retina but are heavily concentrated near the optic nerve head, and to a lesser degree the peripheral retina. Additionally, their morphology is distinct from both nerve fiber layer astrocytes and Müller glia, appearing more similar to amacrine cells. Despite this structural similarity, these cells lack protein expression of common neuronal markers. Additionally, they do not exhibit action potentials, but rather resemble astrocytes and Müller glia in their small amplitude, graded depolarization to both light onset and offset. Their structure, protein expression, physiology, and intercellular connections suggest that these cells are astrocytic, displaced from their counterparts in the nerve fiber layer. As such, we refer to these cells as displaced retinal astrocytes.
Assuntos
Astrócitos , Camundongos Transgênicos , Retina , Animais , Astrócitos/metabolismo , Astrócitos/fisiologia , Retina/citologia , Retina/metabolismo , Retina/fisiologia , Camundongos , Proteína Glial Fibrilar Ácida/metabolismo , Camundongos Endogâmicos C57BL , Potenciais de Ação/fisiologiaRESUMO
Riverine ecosystems have adapted to natural discharge variations across seasons. However, evidence suggesting that climate change has already impacted magnitudes of river flow seasonality is limited to local studies, mainly focusing on changes of mean or extreme flows. This study introduces the use of apportionment entropy as a robust measure to assess flow-volume nonuniformity across seasons, enabling a global analysis. We found that ~21% of long-term river gauging stations exhibit significant alterations in seasonal flow distributions, but two-thirds of these are unrelated to trends in annual mean discharge. By combining a data-driven runoff reconstruction with state-of-the-art hydrological simulations, we identified a discernible weakening of river flow seasonality in northern high latitudes (above 50°N), a phenomenon directly linked to anthropogenic climate forcing.