RESUMO
Mitochondria form a complex, interconnected reticulum that is maintained through coordination among biogenesis, dynamic fission, and fusion and mitophagy, which are initiated in response to various cues to maintain energetic homeostasis. These cellular events, which make up mitochondrial quality control, act with remarkable spatial precision, but what governs such spatial specificity is poorly understood. Herein, we demonstrate that specific isoforms of the cellular bioenergetic sensor, 5' AMP-activated protein kinase (AMPKα1/α2/ß2/γ1), are localized on the outer mitochondrial membrane, referred to as mitoAMPK, in various tissues in mice and humans. Activation of mitoAMPK varies across the reticulum in response to energetic stress, and inhibition of mitoAMPK activity attenuates exercise-induced mitophagy in skeletal muscle in vivo. Discovery of a mitochondrial pool of AMPK and its local importance for mitochondrial quality control underscores the complexity of sensing cellular energetics in vivo that has implications for targeting mitochondrial energetics for disease treatment.
Assuntos
Proteínas Quinases Ativadas por AMP/metabolismo , Metabolismo Energético , Mitocôndrias/patologia , Mitofagia , Condicionamento Físico Animal , Proteínas Quinases Ativadas por AMP/genética , Animais , Humanos , Masculino , Camundongos , Mitocôndrias/metabolismoRESUMO
Heavy rainfall is one of the major aspects of tropical cyclones (TC) and can cause substantial damages. Here, we show, based on satellite observational rainfall data and numerical model results, that between 1999 and 2018, the rain rate in the outer region of TCs has been increasing, but it has decreased significantly in the inner-core. Globally, the TC rain rate has increased by 8 ± 4% during this period, which is mainly contributed by an increase in rain rate in the TC outer region due to increasing water vapor availability in the atmosphere with rising surface temperature. On the other hand, the rain rate in the inner-core of TCs has decreased by 24 ± 3% during the same period. The decreasing trend in the inner-core rain rate likely results mainly from an increase in atmospheric stability.