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1.
Skin Res Technol ; 28(1): 47-53, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34618986

RESUMO

BACKGROUND: An assessment of the drug penetration and distribution profiles within the skin is essential in dermatology and cosmetology. Recent advances in label-free imaging technologies have facilitated the direct detection of unlabeled compounds in tissues, with high resolution. However, it remains challenging to provide quantitative time-course distribution maps of drugs within the complex skin tissue. The present study aims at acquiring the real-time quantitative skin penetration profiles of topically applied caffeine, by means of a combination of pump-probe phase-modulated stimulated Raman scattering (PM-SRS) and confocal reflection microscopy. The recently developed PM-SRS microscopy is a unique imaging tool that can minimize strong background signals through a pulse-shaping technique, while providing high-contrast images of small molecules in tissues. MATERIALS AND METHODS: Reconstructed human skin epidermis models were used in order to analyze caffeine penetration in tissues. The penetration profiles of caffeine in an aqueous solution, an oil-in-water gel, and a water-in-oil gel were examined by combining PM-SRS and confocal reflection microscopy. RESULTS: The characteristic Raman signal of caffeine was directly detected in the skin model using PM-SRS. Integrating PM-SRS and confocal reflection microscopy allowed real-time concentration maps of caffeine to be obtained from formulation samples, within the skin model. Compared with the conventional Raman detection method, PM-SRS lowered the background tissue-oriented signals and supplied high-contrast images of caffeine. CONCLUSION: We successfully established real-time skin penetration profiles of caffeine from different formulations. PM-SRS microscopy proved to be a powerful, non-invasive, and real-time depth-profile imaging technique for use in quantitative studies of topically applied drugs.


Assuntos
Cafeína , Epiderme , Humanos , Microscopia Confocal , Microscopia Óptica não Linear , Pele , Análise Espectral Raman
2.
Faraday Discuss ; 228(0): 312-328, 2021 05 27.
Artigo em Inglês | MEDLINE | ID: mdl-33565544

RESUMO

We discuss our recently reported femtosecond (fs) X-ray emission spectroscopy results on the ligand dissociation and recombination in nitrosylmyoglobin (MbNO) in the context of previous studies on ferrous haem proteins. We also present a preliminary account of femtosecond X-ray absorption studies on MbNO, pointing to the presence of more than one species formed upon photolysis.


Assuntos
Heme , Ligantes , Fotólise , Análise Espectral , Raios X
3.
Biomed Opt Express ; 12(10): 6545-6557, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34745755

RESUMO

Skin penetration analysis of topically applied drugs or active compounds is essential in biomedical applications. Stimulated Raman scattering (SRS) microscopy is a promising label-free skin penetration analysis tool. However, conventional SRS microcopy suffers from limited signal contrast owing to strong background signals, which prevents its use in low-concentration drug imaging. Here, we present a skin penetration analysis method of topical agents using recently developed phase-modulated SRS (PM-SRS) microscopy. PM-SRS uses phase modulation and time-resolved signal detection to suppress both nonlinear background signals and Raman background signals from a tissue. A proof-of-concept experiment with a topically applied skin moisturizing agent (ectoine) in an in vitro skin tissue model revealed that PM-SRS with 1.7-ps probe delay yields a signal contrast 40 times higher than that of conventional amplitude-modulated SRS (AM-SRS). Skin penetration measurement of a topical therapeutic drug (loxoprofen sodium) showed that the mean drug concentration at the tissue surface layer after 240 min was 47.3 ± 4.8 mM. The proposed PM-SRS microscopy can be employed to monitor the spatial and temporal pharmacokinetics of small molecules in the millimolar concentration regime.

4.
Nat Commun ; 11(1): 4145, 2020 08 18.
Artigo em Inglês | MEDLINE | ID: mdl-32811825

RESUMO

In haemoglobin the change from the low-spin (LS) hexacoordinated haem to the high spin (HS, S = 2) pentacoordinated domed deoxy-myoglobin (deoxyMb) form upon ligand detachment from the haem and the reverse process upon ligand binding are what ultimately drives the respiratory function. Here we probe them in the case of Myoglobin-NO (MbNO) using element- and spin-sensitive femtosecond Fe Kα and Kß X-ray emission spectroscopy at an X-ray free-electron laser (FEL). We find that the change from the LS (S = 1/2) MbNO to the HS haem occurs in ~800 fs, and that it proceeds via an intermediate (S = 1) spin state. We also show that upon NO recombination, the return to the planar MbNO ground state is an electronic relaxation from HS to LS taking place in ~30 ps. Thus, the entire ligand dissociation-recombination cycle in MbNO is a spin cross-over followed by a reverse spin cross-over process.


Assuntos
Heme/química , Hemoglobinas/química , Mioglobina/química , Heme/metabolismo , Hemoglobinas/metabolismo , Cinética , Ligantes , Modelos Moleculares , Mioglobina/metabolismo , Espectrometria por Raios X
5.
Struct Dyn ; 4(4): 044033, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28713842

RESUMO

The charge-carrier dynamics of anatase TiO2 nanoparticles in an aqueous solution were studied by femtosecond time-resolved X-ray absorption spectroscopy using an X-ray free electron laser in combination with a synchronized ultraviolet femtosecond laser (268 nm). Using an arrival time monitor for the X-ray pulses, we obtained a temporal resolution of 170 fs. The transient X-ray absorption spectra revealed an ultrafast Ti K-edge shift and a subsequent growth of a pre-edge structure. The edge shift occurred in ca. 100 fs and is ascribed to reduction of Ti by localization of generated conduction band electrons into shallow traps of self-trapped polarons or deep traps at penta-coordinate Ti sites. Growth of the pre-edge feature and reduction of the above-edge peak intensity occur with similar time constants of 300-400 fs, which we assign to the structural distortion dynamics near the surface.

6.
Appl Opt ; 46(23): 5902-11, 2007 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-17694141

RESUMO

The exposure schedule for partially coherent hologram multiplexing, in which data pages are multiplexed by multiple signal beams and a single reference beam, is investigated in detail for the case of a pi/2 phase-shifted photorefractive medium. We found that the optimum recording schedule for partially coherent multiplexing cannot be determined by the classical recording schedule theory because of time-constant errors induced by partially coherent interaction between a reference beam and self-diffraction signal beams. To overcome the issue, we derive a modified recursion equation that accounts for the time-constant errors, and we also propose a novel iterative recording-schedule correction algorism for finding the optimum solution. In the calculation with hologram multiplicity of 30 and photorefractive coupling strength of 3.0, we could successfully obtain a flat diffraction-efficiency profile after the second recursion.

7.
Appl Opt ; 46(13): 2443-52, 2007 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-17429455

RESUMO

We propose a novel phase-conjugate copying method for nondestructive readout of a volatile photorefractive hologram. In the one-crystal configuration, two holographic memories and a mutually pumped phase conjugator (MPPC) are formed within a single photorefractive crystal, instead of using multiple crystals. Two memories share the same hologram and complement each other in refreshing the hologram. A MPPC suppresses fanning noise and automatically aligns the wavefront of the reference and readout beams. We found the optimum configuration to achieve nondestructive readout from calculations and geometric consideration. In the experiments with a BaTiO(3) crystal, a continuous readout of 20 times longer than the recording time was achieved.

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