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1.
Viruses ; 15(2)2023 02 20.
Artigo em Inglês | MEDLINE | ID: mdl-36851796

RESUMO

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people worldwide since its emergence in 2019. Knowing the potential capacity of the virus to adapt to other species, the serological surveillance of SARS-CoV-2 infection in susceptible animals is important. Hong Kong and Seoul are two of Asia's most densely populated urban cities, where companion animals often live in close contact with humans. Sera collected from 1040 cats and 855 dogs during the early phase of the pandemic in Hong Kong and Seoul were tested for SARS-CoV-2 antibodies using an ELISA that detects antibodies against the receptor binding domain of the viral spike protein. Positive sera were also tested for virus neutralizing antibodies using a surrogate virus neutralization (sVNT) and plaque reduction neutralization test (PRNT). Among feline sera, 4.51% and 2.54% of the samples from Korea and Hong Kong, respectively, tested ELISA positive. However, only 1.64% of the samples from Korea and 0.18% from Hong Kong tested positive by sVNT, while only 0.41% of samples from Korea tested positive by PRNT. Among canine samples, 4.94% and 6.46% from Korea and Hong Kong, respectively, tested positive by ELISA, while only 0.29% of sera from Korea were positive on sVNT and no canine sera tested positive by PRNT. These results confirm a low seroprevalence of SARS-CoV-2 exposure in companion animals in Korea and Hong Kong. The discordance between the RBD-ELISA and neutralization tests may indicate possible ELISA cross-reactivity with other coronaviruses, especially in canine sera.


Assuntos
COVID-19 , Doenças do Gato , Doenças do Cão , Gatos , Humanos , Animais , Cães , COVID-19/epidemiologia , COVID-19/veterinária , SARS-CoV-2 , Pandemias , Prevalência , Doenças do Gato/epidemiologia , Hong Kong/epidemiologia , Estudos Soroepidemiológicos , Doenças do Cão/epidemiologia , Anticorpos Antivirais , República da Coreia/epidemiologia
2.
J Vet Sci ; 17(1): 79-87, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-27051343

RESUMO

This study was conducted to identify the effectiveness of platelet-rich plasma (PRP) and efficacy of intralesional injection as a method of application to acute cutaneous wounds in dogs. Healthy adult beagles (n = 3) were used in this study. Autologous PRP was separated from anticoagulant treated whole blood in three dogs. Cutaneous wounds were created and then treated by intralesional injection of PRP in the experimental group, while they were treated with saline in the control group on days 0, 2 and 4. The healing process was evaluated by gross examination throughout the experimental period and histologic examination on day 7, 14 and 21. In PRP treated wounds, the mean diameter was smaller and the wound closure rate was higher than in the control. Histological study revealed that PRP treated wounds showed more granulation formation and angiogenesis on day 7, and faster epithelialization, more granulation formation and collagen deposition were observed on day 14 than in control wounds. On day 21, collagen deposition and epithelialization were enhanced in PRP treated groups. Overall, PRP application showed beneficial effects in wound healing, and intralesional injection was useful for application of PRP and could be a good therapeutic option for wound management in dogs.


Assuntos
Epiderme/fisiologia , Plasma Rico em Plaquetas , Cicatrização , Ferimentos e Lesões/veterinária , Animais , Colágeno/metabolismo , Derme/citologia , Derme/lesões , Derme/fisiologia , Cães , Células Epidérmicas , Epiderme/lesões , Feminino , Tecido de Granulação/citologia , Injeções Intralesionais/veterinária , Masculino , Neovascularização Fisiológica , Regeneração , Resultado do Tratamento , Ferimentos e Lesões/terapia
3.
J Vet Sci ; 16(1): 75-85, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25269716

RESUMO

The purpose of this study was to identify time-related changes in clinical, MRI, histopathologic, and immunohistochemical findings associated with ischemic stroke in dogs. Additionally, the association of cerebrospinal fluid (CSF) and tissue levels of interleukin (IL)-6 with clinical prognosis was assessed. Ischemic stroke was induced by permanent middle cerebral artery occlusion (MCAO) in nine healthy experimental dogs. The dogs were divided into three groups according to survival time and duration of the experimental period: group A (survived only 1 day), group B (1-week experimental period), and group C (2-week experimental period). Neurologic status was evaluated daily. Magnetic resonance imaging (MRI) was performed according to a predetermined schedule. Concentration of IL-6 in CSF was measured serially after ischemic stroke. Postmortem examination was performed for all experimental dogs. During histopathological examination, variable degrees of cavitation and necrosis due to neuronal cytopathic effects, such as pyknotic nuclei and cytoplasmic shrinkage, were observed on the affected side of the cerebral cortex in all dogs. Immunohistochemistry specific for IL-6 showed increased expression in the ischemic lesions. CSF IL-6 concentrations and ischemic lesion volumes 1 day after ischemic stroke were significantly higher in group A compared to groups B and C.


Assuntos
Isquemia Encefálica/etiologia , Imuno-Histoquímica , Infarto da Artéria Cerebral Média , Imageamento por Ressonância Magnética , Acidente Vascular Cerebral/patologia , Animais , Cães , Feminino , Masculino
4.
J Vet Med Sci ; 76(11): 1545-8, 2014 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25131949

RESUMO

An 8-year-old intact male Belgian Malinois, weighing 37.2 kg, was referred for evaluation due to right side facial paresis, ataxia and a 2-month history of decreased cognitive ability. Physical and neurological examinations revealed mild depression, left-sided head tilt, right-sided facial paresis and ataxia. A well-demarcated, broad-based cerebellar mass and hyperostosis were found on CT imaging of the brain. Based on these CT findings, a cerebellar meningioma was strongly suspected. Hydroxyurea and prednisolone were administered; after 4 weeks, there was reduction in mass size as compared to initial CT results. However, the mass size was found to have grown 6 weeks after hydroxyurea treatment. We then prescribed a combination of imatinib mesylate and hydroxyurea. Two weeks following combination treatment, the mass size had reduced significantly. The mass continuously decreased in size until the patient died during anesthesia. Cerebellar transitional meningioma was confirmed by histopathologic examination. To the author's knowledge, this is the first reported case of imatinib mesylate plus hydroxyurea therapy for the treatment of meningioma in veterinary medicine.


Assuntos
Benzamidas/uso terapêutico , Neoplasias Cerebelares/veterinária , Doenças do Cão/diagnóstico por imagem , Doenças do Cão/tratamento farmacológico , Hidroxiureia/uso terapêutico , Meningioma/veterinária , Piperazinas/uso terapêutico , Pirimidinas/uso terapêutico , Animais , Neoplasias Cerebelares/diagnóstico por imagem , Neoplasias Cerebelares/tratamento farmacológico , Cães , Evolução Fatal , Mesilato de Imatinib , Masculino , Meningioma/diagnóstico por imagem , Meningioma/tratamento farmacológico , Prednisolona , Tomografia Computadorizada por Raios X/veterinária , Resultado do Tratamento
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