Test of Electric Charge Conservation with Borexino.

Borexino is a liquid scintillation detector located deep underground at the Laboratori Nazionali del Gran Sasso (LNGS, Italy). Thanks to the unmatched radio purity of the scintillator, and to the well understood detector response at low energy, a new limit on the stability of the electron for decay into a neutrino and a single monoenergetic photon was obtained. This new bound, τ≥6.6×10^{28} yr at 90% C.L., is 2 orders of magnitude better than the previous limit.

HPLC determination of rubber septum contaminants in the iodinated intravenous contrast agent (sodium iothalamate).

A simple and rapid method is described for the determination of 2-mercaptobenzothiazole and mercaptobenzothiazole disulfide leached from rubber closures into the iodinated contrast medium (sodium iothalamate). The contaminants were extracted with methylene chloride and analyzed by high-performance liquid chromatography using ultraviolet detection at wavelengths of 272 and 328 nm. The structures of the impurities were confirmed unequivocally by mass spectrometry. The minimum detectable level was 1 microgram/100 mL (0.01 ppm). The quantitation of contaminants leached from rubber stoppers provides a means for evaluating possible health risks of the extractables.

Determination of 4,4'-methylenebis(2-chloroaniline) in urine using capillary gas chromatography and negative ion chemical ionization mass spectrometry.

A highly sensitive and specific gas chromatographic-mass spectrometric (GC-MS) assay for the determination of 4,4'-methylenebis(2-chloroaniline) (MBOCA) in urine is reported. It is based on the solvent extraction of the hydrolysed MBOCA conjugates, together with deuterium-labelled benzidine-d8 added as an internal standard, and a two-phase derivatization procedure involving use of pentafluoropropionic anhydride in the presence of ammonia as the phase-transfer catalyst. The reaction is complete within 2 min at room temperature. The pentafluoropropyl derivatives are determined by use of capillary column GC-MS with selected-ion monitoring in the negative ion chemical ionization mode. The lower limit of detection for MBOCA was 1 microgram dm-3 and the calibration graph showed linearity between 10 and 250 micrograms dm-3. The recovery of the analyte added to pooled urine was above 86%. Thirty urine specimens from workers employed in a polyurethane-producing plant were analysed for MBOCA by this method.

Gas chromatographic determination of ethyl 2-cyanoacrylate in the workplace environment.

Low levels of airborne ethyl 2-cyanoacrylate (ECA) were monitored by drawing air through sampling tubes containing Tenax GC. After desorption with acetone, ECA was quantified by gas chromatography using a thermionic specific detector in the nitrogen mode. Polymerised ECA was shown to yield ECA monomer under the conditions of gas chromatographic analysis.

Determination of 2-methylaziridine in workplace atmospheres.

A sampling and analytical procedure was developed for the monitoring of airborne 2-methylaziridine (MA). The analyte is collected by drawing air through a solution of 2,4,6-trinitrobenzenesulphonic acid (TNBS). In situ derivatisation of MA with TNBS during sample collection provides stability to the highly reactive analyte and makes it amenable to a sensitive high-performance liquid chromatographic determination with ultraviolet detection. The purified synthetic derivative of MA with TNBS is more suitable as a calibration standard than commercially available MA.