RESUMO
Enhancers are cis-regulatory elements that shape gene expression in response to numerous developmental and environmental cues. In animals, several models have been proposed to explain how enhancers integrate the activity of multiple transcription factors. However, it remains largely unclear how plant enhancers integrate transcription factor activity. Here, we use Plant STARR-seq to characterize 3 light-responsive plant enhancers-AB80, Cab-1, and rbcS-E9-derived from genes associated with photosynthesis. Saturation mutagenesis revealed mutations, many of which clustered in short regions, that strongly reduced enhancer activity in the light, in the dark, or in both conditions. When tested in the light, these mutation-sensitive regions did not function on their own; rather, cooperative interactions with other such regions were required for full activity. Epistatic interactions occurred between mutations in adjacent mutation-sensitive regions, and the spacing and order of mutation-sensitive regions in synthetic enhancers affected enhancer activity. In contrast, when tested in the dark, mutation-sensitive regions acted independently and additively in conferring enhancer activity. Taken together, this work demonstrates that plant enhancers show evidence for both cooperative and additive interactions among their functional elements. This knowledge can be harnessed to design strong, condition-specific synthetic enhancers.
Assuntos
Arabidopsis , Elementos Facilitadores Genéticos , Regulação da Expressão Gênica de Plantas , Arabidopsis/genética , Arabidopsis/metabolismo , Mutação , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Fatores de Transcrição/metabolismo , Fatores de Transcrição/genética , Epistasia Genética , LuzRESUMO
The 3' end of a gene, often called a terminator, modulates mRNA stability, localization, translation, and polyadenylation. Here, we adapted Plant STARR-seq, a massively parallel reporter assay, to measure the activity of over 50,000 terminators from the plants Arabidopsis thaliana and Zea mays. We characterize thousands of plant terminators, including many that outperform bacterial terminators commonly used in plants. Terminator activity is species-specific, differing in tobacco leaf and maize protoplast assays. While recapitulating known biology, our results reveal the relative contributions of polyadenylation motifs to terminator strength. We built a computational model to predict terminator strength and used it to conduct in silico evolution that generated optimized synthetic terminators. Additionally, we discover alternative polyadenylation sites across tens of thousands of terminators; however, the strongest terminators tend to have a dominant cleavage site. Our results establish features of plant terminator function and identify strong naturally occurring and synthetic terminators.
Assuntos
Arabidopsis , Poliadenilação , Zea mays , Zea mays/genética , Zea mays/metabolismo , Arabidopsis/genética , Arabidopsis/metabolismo , Regulação da Expressão Gênica de Plantas , Regiões Terminadoras Genéticas/genética , Nicotiana/genética , Nicotiana/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismoRESUMO
Deep learning has become a popular tool to study cis-regulatory function. Yet efforts to design software for deep-learning analyses in regulatory genomics that are findable, accessible, interoperable and reusable (FAIR) have fallen short of fully meeting these criteria. Here we present elucidating the utility of genomic elements with neural nets (EUGENe), a FAIR toolkit for the analysis of genomic sequences with deep learning. EUGENe consists of a set of modules and subpackages for executing the key functionality of a genomics deep learning workflow: (1) extracting, transforming and loading sequence data from many common file formats; (2) instantiating, initializing and training diverse model architectures; and (3) evaluating and interpreting model behavior. We designed EUGENe as a simple, flexible and extensible interface for streamlining and customizing end-to-end deep-learning sequence analyses, and illustrate these principles through application of the toolkit to three predictive modeling tasks. We hope that EUGENe represents a springboard towards a collaborative ecosystem for deep-learning applications in genomics research.