RESUMO
OBJECTIVES: To evaluate the role of serotonin in the development of a biomimetic enamel-like material in vitro. SETTING AND SAMPLE POPULATION: Immortalized murine oral keratinocytes raised from adult mouse mucosa were cultured in vitro. In addition, specimens incorporating molar tooth buds harvested from mice were included in our studies. MATERIALS AND METHODS: We used cell-based scaffold-free tissue engineering to assemble three-dimensional (3D) organoids into complex tissue constructs that closely emulate the complexity of adult enamel. We also analysed mouse molar specimens using immunohistochemistry for serotonin expression as well as its cognate receptor. RESULTS: TGF-ß1-reprogrammed murine oral keratinocytes formed organoids that laid down an amelogenin-rich protein matrix when grown as three-dimensional (3D) cultures in the presence of serotonin. Following mineralization, the newly formed crystals were densified under pressure and vacuum to produce a biomimetic enamel-like material that demonstrated parallel alignment of hydroxyapatite crystals with some interspaced residual organoid matter into enamel prism-like structures conferring size, mechanical properties comparable to tooth enamel, including light translucency. Serotonin expression was localized by immunohistochemistry proximal to the enamel organ of developing molar buds. Moreover, serotonin HTRb2 receptor expression was localized on ameloblasts within the enamel organ proximal to the area where serotonin was immunolocalized. CONCLUSIONS: Our results demonstrate that serotonin is inductive in the development of a biomimetic enamel-like material from reprogrammed oral epithelial keratinocytes in vitro. The facileness of harvesting adult somatic cells together with the versatility of our approach offers exciting opportunities to address regenerative challenges linked to lost enamel.
Assuntos
Biomimética , Serotonina , Amelogenina , Animais , Esmalte Dentário , Queratinócitos , CamundongosRESUMO
INTRODUCTION: Bone remodeling is crucial to the success of many dental procedures and is tightly regulated. Vascular endothelial growth factor (VEGF), a key cytokine for angiogenesis, is also an important regulator of bone remodeling. We aimed to examine the mechanisms by which VEGF induces bone remodeling by studying its effects on cultured osteoblasts and osteoclasts. METHODS: Preosteoblastic MC3T3-E1 cells were treated with vehicle or VEGF-A165. Cell proliferation, migration, and invasion potentials were assessed. Preosteoclastic RAW264.7 cells were treated with vehicle or VEGF with or without the receptor activator of nuclear factor kappa-B ligand (RANKL), and osteoclast formation was measured with tartrate-resistant acid phosphatase staining. Conditioned media from vehicle-treated or VEGF-treated MC3T3-E1 cells were tested for the levels of RANKL and osteoprotegerin (OPG) and were used to treat RAW264.7 cells to observe osteoclast formation. RESULTS: VEGF significantly induced MC3T3-E1 cell proliferation, migration, and invasion. VEGF did not directly induce osteoclastogenesis but significantly increased the RANKL/OPG ratio in the conditioned media from the MC3T3-E1 cultures; this significantly increased osteoclast formation. CONCLUSIONS: VEGF stimulates osteoclast differentiation by increasing the osteoblastic RANKL/OPG ratio but has no direct effect on osteoclast precursor cells, and it induces osteoblast proliferation, migration, and invasion potentials in vitro.
Assuntos
Osteoblastos/efeitos dos fármacos , Osteoclastos/efeitos dos fármacos , Fator A de Crescimento do Endotélio Vascular/farmacologia , Células 3T3 , Fosfatase Ácida/análise , Animais , Remodelação Óssea/efeitos dos fármacos , Contagem de Células , Técnicas de Cultura de Células , Diferenciação Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Corantes , Meios de Cultivo Condicionados , Isoenzimas/análise , Macrófagos/efeitos dos fármacos , Camundongos , Osteoprotegerina/análise , Ligante RANK/análise , Ligante RANK/farmacologia , Fosfatase Ácida Resistente a TartaratoRESUMO
Neuroinflammation is an important component of Alzheimer's disease (AD) pathogenesis and has been implicated in neurodegeneration. Interleukin-1 (IL-1), a potent inflammatory cytokine in the CNS, is chronically upregulated in human AD and believed to serve as part of a vicious inflammatory cycle that drives AD pathology. To further understand the role of IL-1ß in AD pathogenesis, we used an inducible model of sustained IL-1ß overexpression (IL-1ß(XAT)) developed in our laboratory. The triple transgenic mouse model of AD, which develops plaques and tangles later in its life cycle, was bred with IL-1ß(XAT) mice, and effects of IL-1ß overexpression on AD pathology were assessed in F1 progeny. After 1 and 3 months of transgene expression, we found robust increases in tau phosphorylation despite an â¼70-80% reduction in amyloid load and fourfold to sixfold increase in plaque-associated microglia, as well as evidence of greater microglial activation at the site of inflammation. We also found evidence of increased p38 mitogen-activated protein kinase and glycogen synthase kinase-3ß activity, which are believed to contribute to tau phosphorylation. Thus, neuroinflammation regulates amyloid and tau pathology in opposing ways, suggesting that it provides a link between amyloid accumulation and changes in tau and raising concerns about the use of immunomodulatory therapies in AD.
Assuntos
Doença de Alzheimer/metabolismo , Doença de Alzheimer/patologia , Peptídeos beta-Amiloides/metabolismo , Encéfalo/patologia , Interleucina-1beta/metabolismo , Proteínas tau/metabolismo , Fatores Etários , Doença de Alzheimer/genética , Secretases da Proteína Precursora do Amiloide/metabolismo , Precursor de Proteína beta-Amiloide/genética , Análise de Variância , Animais , Encéfalo/metabolismo , Modelos Animais de Doenças , Ensaio de Imunoadsorção Enzimática , Regulação da Expressão Gênica/genética , Proteína Glial Fibrilar Ácida/metabolismo , Quinase 3 da Glicogênio Sintase/metabolismo , Humanos , Vírus da Imunodeficiência Felina/genética , Interleucina-1beta/deficiência , Sistema de Sinalização das MAP Quinases , Camundongos , Camundongos Endogâmicos C57BL , Proteínas dos Microfilamentos , Proteínas Musculares , Mutação/genética , Presenilina-1/genética , Trissacarídeos/metabolismo , Tubulina (Proteína)/metabolismo , Proteínas tau/genéticaRESUMO
Recent literature suggests that sEcad exerts pro-oncogenic effects, possibly acting as a ligand for the human epidermal growth factor family. Here we show that sEcad is a novel candidate protein for drug targeting since it is increased in human and mouse HER2-positive (HER2+) breast tumors, MMTV-PyMT bodily fluids and human cell culture systems. Mechanistically, we show that endogenous sEcad, and to a lesser extent membrane-bound E-cadherin, associates with HER1, HER2, and HER3 in human and MMTV-PyMT mouse HER2+ tumors and with HER1 in triple negative breast cancer (TNBC) specimens. Furthermore, addition of exogenous recombinant human E-cadherin/Fc chimeric protein (rhEcad/Fc; sEcad) to HER2+ MCF-7, SKBR3, and HER2-negative MDA-MB-231 TNBC cells, resulted in sEcad-HER receptor family interactions, activation of HER1-4 and downstream pro-survival signaling, including the MAPK-PI3K/Akt/mTOR pathways and IAP family members. Lastly, we demonstrate that sEcad exerts pro-oncogenic effects via HER signaling, and acts additively with the HER ligand EGF to promote HER2+ breast cancer proliferation and migration, as well as TNBC invasion. Because sEcad associates and activates many of the oncogenic pathways that tumors utilize for growth and survival and serum levels in patients correlates with clinical response, suggests that targeted therapy against sEcad in combination with other therapies may potentially offer a novel therapeutic strategy for the treatment of breast cancers.
Assuntos
Caderinas/metabolismo , Receptores ErbB/biossíntese , Terapia de Alvo Molecular , Receptor ErbB-2/biossíntese , Receptor ErbB-3/biossíntese , Neoplasias de Mama Triplo Negativas/metabolismo , Neoplasias de Mama Triplo Negativas/patologia , Idoso , Animais , Caderinas/antagonistas & inibidores , Caderinas/farmacologia , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Receptores ErbB/metabolismo , Feminino , Humanos , Células MCF-7 , Camundongos , Pessoa de Meia-Idade , Invasividade Neoplásica , Fosfatidilinositol 3-Quinase/biossíntese , Proteínas Proto-Oncogênicas c-akt/biossíntese , Receptor ErbB-2/metabolismo , Receptor ErbB-3/metabolismo , Transdução de Sinais , Serina-Treonina Quinases TOR/biossíntese , Neoplasias de Mama Triplo Negativas/tratamento farmacológicoRESUMO
Accelerating orthodontic tooth movement can significantly reduce treatment duration and risks of side effects. The rate of orthodontic tooth movement is chiefly determined by the remodeling of tissues surrounding the roots; this in turn is under the control of molecular mechanisms regulating cellular behaviors in the alveolar bone and periodontal ligament. This review summarizes the current knowledge on the molecular mechanisms underlying accelerated orthodontic tooth movement, and the clinical and experimental methods that accelerate orthodontic tooth movement with possible molecular mechanisms. The review also shows directions for future studies to develop more clinically applicable methods to accelerate orthodontic tooth movement.
Assuntos
Técnicas de Movimentação Dentária/métodos , Processo Alveolar/fisiologia , Remodelação Óssea/fisiologia , Humanos , Biologia Molecular , Osteoblastos/fisiologia , Osteoclastos/fisiologia , Osteócitos/fisiologia , Ligamento Periodontal/fisiologiaRESUMO
This study evaluated whether GM(2) ganglioside storage is necessary for neurodegeneration and neuroinflammation by performing ß-hexosaminidase rescue experiments in neurons of HexB(-/-) mice. We developed a novel mouse model, whereby the expression of the human HEXB gene was targeted to neurons of HexB(-/-) mice by the Thy1 promoter. Despite ß-hexosaminidase restoration in neurons was sufficient in rescuing HexB(-/-) mice from GM(2) neuronal storage and neurodegeneration, brain inflammation persisted, including the presence of large numbers of reactive microglia/macrophages due to persisting GM(2) presence in this cell type. In conclusion, our results suggest that neuroinflammation is not sufficient to elicit neurodegeneration as long as neuronal function is restored.
Assuntos
Encéfalo/enzimologia , Regulação Enzimológica da Expressão Gênica , Doenças Neurodegenerativas/enzimologia , Neurônios/enzimologia , Doença de Sandhoff/enzimologia , beta-N-Acetil-Hexosaminidases/genética , Animais , Encéfalo/patologia , Humanos , Inflamação/enzimologia , Inflamação/patologia , Camundongos , Camundongos da Linhagem 129 , Camundongos Transgênicos , Doenças Neurodegenerativas/patologia , Neurônios/patologia , Doença de Sandhoff/genética , Doença de Sandhoff/patologia , beta-N-Acetil-Hexosaminidases/biossínteseRESUMO
OBJECTIVES: The goal of this project was to evaluate the use of two adeno-associated viral vector serotypes, adeno-associated viral vectors (AAV)-2 and AAV-6, approved for and used for gene therapy in humans, for the delivery of therapeutic genes to the temporomandibular joint (TMJ) and the attendant sensory nerves. METHODS: Young adult wild-type C57BL/6 mice were intra-articularly inoculated with AAV-2 and AAV-6 encoding the reporter gene gfp, the expression of which was assessed in the TMJ as well as along nerves innervating the TMJ. RESULTS: AAV-2 and AAV-6 serotypes were characterized by varying levels of tissue tropism demonstrating different efficacy of infection for articular chondrocytes, meniscal fibroblasts, and trigeminal neurons. Specifically, AAV-2 infected both neurons and articular chondrocytes/meniscal fibroblasts, whereas AAV-6 showed selectivity primarily for neurons. CONCLUSIONS: The results of this study are clinically significant in the successful application of gene therapy vectors for TMJ disorders, as this new knowledge will allow for appropriate targeting of specific therapeutic genes to selective tissues (neurons vs. chondrocytes/fibroblasts) as needed by using specific viral vector serotypes.
Assuntos
Terapia Genética , Articulação Temporomandibular , Humanos , Camundongos , Animais , Adulto Jovem , Transdução Genética , Camundongos Endogâmicos C57BL , Terapia Genética/métodosRESUMO
OBJECTIVES: We set out to investigate whether a hybrid stem-like p-EMT phenotype develops during murine molar enamel development in vivo. SETTING AND SAMPLE POPULATION: Histology specimens incorporating molar tooth buds harvested from mice at post-natal day 4 (P4) were included in our studies. MATERIALS AND METHODS: We employed double immunofluorescence staining to analyze the simultaneous expression of the epithelial marker E-cadherin and the mesenchymal marker N-cadherin in histology specimens with tooth buds harvested from P4 mice. Moreover, we evaluated the expression of the core master stem cell markers Oct4 and Sox2, as well as the mature ameloblast marker amelogenin. RESULTS: Here we document the co-expression of E-cadherin and N-cadherin in a sub-population of pre-ameloblasts in the inner enamel epithelium suggestive of the presence of a hybrid epithelial/mesenchymal phenotype resulting from p-EMT. Moreover, the core stem cell factors Oct4 and Sox2 colocalized with E-cadherin expressing pre-ameloblasts, whereas the mesenchymal marker N-cadherin was expressed specifically by amelogenin-positive mature secretory ameloblasts. CONCLUSIONS: The differentiation of E-cadherin-positive pre-ameloblasts towards N-cadherin-positive mature secretory ameloblasts transition through a previously unidentified epithelial/mesenchymal stage derived through p-EMT, co-expressing the master transcription factors Oct4 and Sox2.
Assuntos
Ameloblastos , Transição Epitelial-Mesenquimal , Ameloblastos/metabolismo , Amelogenina/genética , Amelogenina/metabolismo , Animais , Caderinas/metabolismo , Esmalte Dentário , CamundongosRESUMO
OBJECTIVES: The objective of this study is to confirm the developmental origin of the enamel organ and evaluate the role of E-cadherin in tooth development by conditional deletion in the oral epithelium and its enamel organ progeny. K5-Cre;ROSA26 compound mice were included in this study in order to confirm the oral epithelial origin of the enamel organ, as well as of the action of the K5-Cre transgene in ablating E-cadherin in the enamel organ. K5-Cre;Ecadfl/fl knockout mice were included to evaluate the effects of the conditional E-cadherin ablation onto tooth development. MATERIAL AND METHODS: K5-Cre transgenic mice were crossed into the ROSA26 reporter mouse to trace the cell fate of the oral epithelium and its progeny in vivo. Moreover, K5-Cre mice were crossed into the Ecadfl/fl mice to produce K5-Cre;Ecadfl/fl compound mouse, as well as K5-Cre;Ecadfl/+ and Ecadfl/fl littermate controls. These litters were euthanized at postnatal day P2 to study the effects of conditional E-cadherin ablation in vivo. RESULTS: The K5-Cre;ROSA26 compound mouse demonstrated that the origin of the enamel organ and the structures thereof are of oral epithelial origin. Furthermore, using the K5-Cre;Ecadfl/fl compound mouse, we determined that conditional ablation of E-cadherin in the oral epithelium, and its progeny, results in dental anomalies involving elongation of the molar root, shrinkage of the pulp space, and alterations of the periapical area, including cementum hyperplasia. The K5-Cre;Ecadfl/fl mice also displayed a smaller overall stature compared with heterozygotes and wild-type littermates. CONCLUSIONS: E-cadherin is important in tooth development, including the formation of enamel, the crown, pulp space, and the roots.
Assuntos
Odontogênese , Dente , Animais , Caderinas/genética , Esmalte Dentário , Epitélio , Camundongos , Camundongos Knockout , Odontogênese/genéticaRESUMO
OBJECTIVES: The goal of this project was to evaluate the role of calcitonin gene-related peptide (CGRP) in the development of arthritis. METHODS: Herein, we employed somatic mosaic analysis in two different joints by FIV(CGRP) intra-articular inoculation in the knees or temporomandibular joints (TMJ) of young adult male C57/BL6 mice. FIV(CGRP) is a feline immunodeficiency virus over-expressing full-length CGRP. Joint pathology and function were evaluated at the histopathological and behavioral levels. In addition, CGRP signaling was inhibited by intra-articular inoculation using FIV(CGRP8-37 ), such that the inhibitory peptide CGRP(8-37) was overexpressed 4 weeks after induction of joint inflammation in the TMJ of IL-1ßXAT transgenic mouse model. The mice were evaluated for behavior and killed for evaluation of knee and TMJ pathology. RESULTS: Overexpression of CGRP in the joints of wild-type mice induced the development of joint anomalies, including meniscal hypertrophy and articular pathology, associated with nocifensive behavior. Intriguingly, overexpression of the CGRP(8-37) inhibitory peptide in the knee and TMJ of IL-1ßXAT transgenic mice with joint inflammation resulted in partial amelioration of the attendant joint pathology. CONCLUSIONS: The results of this study suggest that CGRP is sufficient and necessary for the development of joint pathology and may serve as an intra-articular therapeutic target using gene therapy or monoclonal antibody-based therapies.
Assuntos
Peptídeo Relacionado com Gene de Calcitonina , Transtornos da Articulação Temporomandibular , Animais , Anticorpos Monoclonais , Peptídeo Relacionado com Gene de Calcitonina/genética , Inflamação , Masculino , Camundongos , Camundongos Transgênicos , Articulação Temporomandibular , Transtornos da Articulação Temporomandibular/tratamento farmacológico , Transtornos da Articulação Temporomandibular/genética , Transtornos da Articulação Temporomandibular/patologiaRESUMO
BACKGROUND: The purpose of this study was to investigate whether localized peripheral inflammation, such as osteoarthritis, contributes to neuroinflammation and neurodegenerative disease in vivo. METHODS: We employed the inducible Col1-IL1ßXAT mouse model of osteoarthritis, in which induction of osteoarthritis in the knees and temporomandibular joints resulted in astrocyte and microglial activation in the brain, accompanied by upregulation of inflammation-related gene expression. The biological significance of the link between peripheral and brain inflammation was explored in the APP/PS1 mouse model of Alzheimer's disease (AD) whereby osteoarthritis resulted in neuroinflammation as well as exacerbation and acceleration of AD pathology. RESULTS: Induction of osteoarthritis exacerbated and accelerated the development of neuroinflammation, as assessed by glial cell activation and quantification of inflammation-related mRNAs, as well as Aß pathology, assessed by the number and size of amyloid plaques, in the APP/PS1; Col1-IL1ßXAT compound transgenic mouse. CONCLUSION: This work supports a model by which peripheral inflammation triggers the development of neuroinflammation and subsequently the induction of AD pathology. Better understanding of the link between peripheral localized inflammation, whether in the form of osteoarthritis, atherosclerosis or other conditions, and brain inflammation, may prove critical to our understanding of the pathophysiology of disorders such as Alzheimer's, Parkinson's and other neurodegenerative diseases.
Assuntos
Doença de Alzheimer/etiologia , Doença de Alzheimer/patologia , Inflamação/complicações , Inflamação/imunologia , Osteoartrite/complicações , Osteoartrite/patologia , Doença de Alzheimer/fisiopatologia , Peptídeos beta-Amiloides/metabolismo , Animais , Comportamento Animal/fisiologia , Encéfalo/imunologia , Encéfalo/patologia , Encéfalo/fisiopatologia , Colágeno Tipo I/genética , Colágeno Tipo I/imunologia , Modelos Animais de Doenças , Humanos , Inflamação/patologia , Interleucina-1beta/genética , Interleucina-1beta/imunologia , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Osteoartrite/fisiopatologia , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/imunologiaRESUMO
OBJECTIVES: We set out to reprogram adult somatic oral epithelial keratinocytes into pluripotent cells for regenerative dentistry. SETTING AND SAMPLE POPULATION: Immortalized murine oral keratinocyte cell (IMOK) line raised from adult mouse mucosa were cultured in vitro in our studies. MATERIALS AND METHODS: Adult murine oral epithelial keratinocytes were chronically treated with TGF-ß1 in vitro, and the expression of Oct4, Nanog, Sox2 and Nestin, as well as specific homeobox Gata and Pax gene family members were investigated. RESULTS: We documented the induction of stem factors linked with pluripotency and/or the maintenance and regulation of stem-cell self-renewal in oral epithelial keratinocytes by TGFß1. Moreover, we discovered that this TGF-ß1-induced increase in Oct4, Nanog, Sox2 and Nestin was inhibited by SB431542, suggesting that TGF-ß1 signals via the TGF-ßRI receptor to induce pluripotency and stemness. CONCLUSIONS: Adult oral epithelial keratinocytes treated chronically with TGF-ß1 acquired phenotypic characteristics consistent with pluripotent stem cells, highlighting the facileness of reprogramming adult oral keratinocytes into an unlimited supply of pluripotent stem cells.
Assuntos
Células-Tronco Pluripotentes , Animais , Queratinócitos , Camundongos , Fenótipo , Células-Tronco Pluripotentes/metabolismoRESUMO
Interleukin (IL)-1beta is a proinflammatory cytokine implicated in several neurodegenerative disorders. Downstream actions of IL-1beta include production of prostaglandin (PG) E(2) by increasing expression of cyclooxygenase (COX) enzymes and prostaglandin E synthase (PGES) isoforms. We recently developed a transgenic mouse carrying a dormant human IL-1beta eXcisional Activation Transgene (XAT) for conditional and chronic up-regulation of IL-1beta in selected brain regions. This model is characterized by regionally specific glial activation, immune cell recruitment, and induction of cytokines and chemokines. Here, we aimed to elucidate the effects of long-term IL-1beta expression on the PGE(2) synthetic pathway and to determine the effects of PGs on inflammation and memory in our model. As expected, PGE(2) levels were significantly elevated after IL-1beta up-regulation. Quantitative real-time PCR analysis indicated significant induction of mRNAs for COX-1 and membranous PGES-1, but not COX-2 or other PGES isoforms. Immunohistochemistry revealed elevation of COX-1 but no change in COX-2 following sustained IL-1beta production. Furthermore, pharmacological inhibition of COX-1 and use of COX-1 knockout mice abrogated IL-1beta-mediated PGE(2) increases. Although COX-1 deficient mice did not present a dramatically altered neuroinflammatory phenotype, they did exhibit improved contextual fear memory. This data suggests a unique role for COX-1 in mediating chronic neuroinflammatory effects through PGE(2) production.
Assuntos
Ciclo-Oxigenase 1/fisiologia , Dinoprostona/metabolismo , Hipocampo/metabolismo , Interleucina-1beta/biossíntese , Memória , Animais , Ciclo-Oxigenase 1/genética , Inibidores de Ciclo-Oxigenase/farmacologia , Medo , Humanos , Interleucina-1beta/genética , Camundongos , Camundongos Transgênicos , Modelos AnimaisRESUMO
Neuroinflammation is a conspicuous feature of Alzheimer disease (AD) pathology and is thought to contribute to the ultimate neurodegeneration that ensues. IL-1 beta has emerged as a prime candidate underlying this response. Here we describe a transgenic mouse model of sustained IL-1 beta overexpression that was capable of driving robust neuroinflammation lasting months after transgene activation. This response was characterized by astrocytic and microglial activation in addition to induction of proinflammatory cytokines. Surprisingly, when triggered in the hippocampus of the APPswe/PS1dE9 mouse model of AD, 4 weeks of IL-1 beta overexpression led to a reduction in amyloid pathology. Congophilic plaque area fraction and frequency as well as insoluble amyloid beta 40 (A beta 40) and A beta 42 decreased significantly. These results demonstrate a possible adaptive role for IL-1 beta-driven neuroinflammation in AD and may help explain recent failures of antiinflammatory therapeutics for this disease.
Assuntos
Doença de Alzheimer/terapia , Hipocampo/imunologia , Interleucina-1beta/genética , Interleucina-1beta/uso terapêutico , Doença de Alzheimer/genética , Doença de Alzheimer/imunologia , Doença de Alzheimer/patologia , Precursor de Proteína beta-Amiloide/genética , Animais , Sequência de Bases , Primers do DNA/genética , Modelos Animais de Doenças , Expressão Gênica , Hipocampo/patologia , Humanos , Inflamação/imunologia , Inflamação/patologia , Camundongos , Camundongos Transgênicos , Degeneração Neural/imunologia , Placa Amiloide/patologia , Presenilina-1/genética , Proteínas Recombinantes/genética , Proteínas Recombinantes/uso terapêuticoRESUMO
The incidence of skin cancer is on the rise, with over 1 million new cases yearly. Although it is known that squamous cell cancers (SCC) are caused by UV light, the mechanism(s) involved remains poorly understood. In vitro studies with epithelial cells or reports examining malignant skin lesions suggest that loss of E-cadherin-mediated cell-cell contacts may contribute to SCCs. Other studies show a pivotal role for cyclooxygenase-dependent prostaglandin E2 (PGE2) synthesis in this process. Using chronically UV-irradiated SKH-1 mice, we show a sequential loss of E-cadherin-mediated cell-cell contacts as lesions progress from dysplasia to SCCs. This E-cadherin down-regulation was also evident after acute UV exposure in vivo. In both chronic and acute UV injury, E-cadherin levels declined at a time when epidermal PGE2 synthesis was enhanced. Inhibition of PGE2 synthesis by indomethacin in vitro, targeted deletion of EP2 in primary mouse keratinocyte (PMK) cultures or deletion of the EP2 receptor in vivo abrogated this UV-induced E-cadherin down-regulation. In contrast, addition of PGE2 or the EP2 receptor agonist butaprost to PMK produced a dose- and time-dependent decrease in E-cadherin. We also show that UV irradiation, via the PGE2-EP2 signaling pathway, may initiate tumorigenesis in keratinocytes by down-regulating E-cadherin-mediated cell-cell contacts through its mobilization away from the cell membrane, internalization into the cytoplasm, and shuttling through the lysosome and proteasome degradation pathways. Further understanding of how UV-PGE2-EP2 down-regulates E-cadherin may lead to novel chemopreventative strategies for the treatment of skin and other epithelial cancers.
Assuntos
Caderinas/metabolismo , Carcinoma de Células Escamosas/metabolismo , Dinoprostona/metabolismo , Processamento de Proteína Pós-Traducional , Receptores de Prostaglandina E/metabolismo , Neoplasias Cutâneas/metabolismo , Animais , Caderinas/biossíntese , Caderinas/deficiência , Caderinas/genética , Carcinoma de Células Escamosas/etiologia , Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/patologia , Processos de Crescimento Celular/fisiologia , Dinoprostona/biossíntese , Progressão da Doença , Regulação para Baixo , Regulação Neoplásica da Expressão Gênica , Lisossomos/metabolismo , Camundongos , Camundongos Pelados , Camundongos Knockout , Complexo de Endopeptidases do Proteassoma/metabolismo , Receptores de Prostaglandina E Subtipo EP2 , Neoplasias Cutâneas/etiologia , Neoplasias Cutâneas/genética , Neoplasias Cutâneas/patologia , Raios UltravioletaRESUMO
The proinflammatory cytokine interleukin-1beta (IL-1beta) plays a significant role in leukocyte recruitment to the CNS. Although acute effects of IL-1beta signaling in the mouse brain have been well described, studies elucidating the downstream effects of sustained upregulation have been lacking. Using the recently described IL-1beta(XAT) transgenic mouse model, we triggered sustained unilateral hippocampal overexpression of IL-1beta. Transgene induction led to blood-brain barrier leakage, induction of MCP-1 (monocyte chemoattractant protein 1) (CCL2), ICAM-1 (intercellular adhesion molecule 1), and dramatic infiltration of CD45-positive leukocytes comprised of neutrophils, T-cells, macrophages, and dendritic cells. Despite prolonged cellular infiltration of the hippocampus, there was no evidence of neuronal degeneration. Surprisingly, neutrophils were observed in the hippocampal parenchyma as late as 1 year after transgene induction. Their presence was coincident with upregulation of the potent neutrophil chemotactic chemokines KC (keratinocyte-derived chemokine) (CXCL1) and MIP-2 (macrophage inflammatory protein 2) (CXCL2). Knock-out of their sole receptor CXCR2 abrogated neutrophil infiltration but failed to reduce leakage of the blood-brain barrier.
Assuntos
Barreira Hematoencefálica/fisiologia , Hipocampo/metabolismo , Interleucina-1beta/metabolismo , Leucócitos/metabolismo , Neutrófilos/metabolismo , Regulação para Cima/fisiologia , Animais , Quimiocina CCL2/metabolismo , Quimiocina CXCL2 , Molécula 1 de Adesão Intercelular/metabolismo , Interleucina-1beta/genética , Antígenos Comuns de Leucócito/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Monocinas/deficiência , Monocinas/metabolismoRESUMO
Myeloid-derived immune cells, including microglia, macrophages and monocytes, have been previously implicated in neurodegeneration. We investigated the role of infiltrating peripheral blood mononuclear cells (PBMC) in neuroinflammation and neurodegeneration in the HexB-/- mouse model of Sandhoff disease. Ablation of the chemokine receptor CCR2 in the HexB-/- mouse resulted in significant inhibition of PBMC infiltration into the brain, decrease in TNFalpha and MHC-II mRNA abundance and retardation in clinical disease development. There was no change in the level of GM2 storage and pro-apoptotic activity or astrocyte activation in HexB-/-; Ccr2-/- double knockout mice, which eventually succumbed secondary to GM2 gangliosidosis.
Assuntos
Encefalite/imunologia , Hexosaminidase B/imunologia , Leucócitos Mononucleares/imunologia , Degeneração Neural/imunologia , Doença de Sandhoff/imunologia , Animais , Apoptose/imunologia , Modelos Animais de Doenças , Encefalite/metabolismo , Encefalite/patologia , Feminino , Gangliosídeo G(M2)/metabolismo , Hexosaminidase B/genética , Leucócitos Mononucleares/patologia , Masculino , Camundongos , Camundongos Knockout , Microglia/imunologia , Microglia/patologia , Degeneração Neural/metabolismo , Degeneração Neural/patologia , Receptores CCR2/genética , Receptores CCR2/imunologia , Doença de Sandhoff/metabolismo , Doença de Sandhoff/patologiaRESUMO
We aimed to evaluate the efficacy of VSV-G pseudotyped, defective HIV-1 based lentiviral vectors for the neonatal transfer of therapeutic genes following systemic administration in Sandhoff mouse pups. Despite transgene expression in mouse brains, these animals presented with significant exacerbation and acceleration of the disease neurological phenotype. We observed an increase and acceleration in the presence of MHC-II and CD45+ cells in their brains, along with neuroinflammation, but not in control heterozygous or wild type littermates that also received the same treatment.