Photonic-assisted high-order vector millimeter-wave signal generation enabled by DSM.

We propose an optical dual-single-sideband (dual-SSB) modulated 16384-quadrature amplitude modulation (QAM) photonic vector millimeter-wave (mm-wave) signal generation scheme based on delta-sigma modulation (DSM). With the aid of the DSM, the severe nonlinear distortion of envelope detection for high-order QAM modulation signals in wireless communication can be effectively resolved. For the validation of our proposed scheme, we experimentally demonstrate the generation of a 40 GHz 16384-QAM orthogonal frequency division multiplexing (OFDM) photonic vector mm-wave signal and transmission over a 25-km standard single-mode fiber (SSMF), and a 1-m wireless link with the bit error ratio (BER) reaches the hard-decision forward-error-correction (HD-FEC) threshold of 3.8 × 10-3.

Relationship between myopia control and amount of corneal refractive change after orthokeratology lens treatment.

BACKGROUND: To evaluate the relationship between amount of corneal refractive change (CRC) after wearing orthokeratology (Ortho-K) lenses and axial length (AL) growth. METHODS: We retrospectively enrolled 77 patients (77 eyes) aged 8-14 years who wore Ortho-K lenses more than 12 months. We divided the patients into 2 subgroups: spherical equivalent (SE) ≤ -3.0 D and SE > -3.0 D subgroup. The sagittal and tangential curvature maps and corneal topographic data within the 8-mm diameter ring at the baseline and during follow-up visits after wearing Ortho-K lens were recorded in addition to the area, height, and volume of the CRC region. The AL data were recorded at the baseline and during follow-up visits. Multivariate linear regression was conducted to analyze associations between the area, height, and volume of the CRC region, AL elongation, and SE. RESULTS: The average change in the CRC region was 9.77 ± 0.60 D in height, 16.66 ± 3.61 mm2 in area, and 87.47 ± 8.96 D*mm2 in volume on the tangential diagram after wearing Ortho-K lenses for 3 months. The AL showed a change of 0.19 ± 0.14 mm after 1 year of Ortho-K lens wear (P < 0.05). At 1 year, AL elongation was negatively correlated with the area (P = 0.019) and volume (P < 0.001) of the CRC region. At 1 year, for every 1-mm2 increase in the area and every 1-D*mm2 increase in the volume of the CRC region, the average AL elongation decreased by 0.01 mm and 0.002 mm, respectively, in the multivariate analysis. In patients with SE ≤ -3.0 D, AL elongation was negatively correlated with the CRC-region volume (ß = -0.002, P = 0.018), and in patients with SE > -3.0 D, AL elongation was negatively correlated with the CRC-region area (ß = -0.017, P = 0.016). CONCLUSIONS: The AL elongation-control efficacy of Ortho-K lenses may be related to the area and volume of the CRC region.

Digital mobile fronthaul based on delta-sigma modulation employing a simple self-coherent receiver.

The coherent digital radio-over-fiber (DRoF) system is a promising candidate for future mobile fronthaul networks (MFNs) due to its high receiver sensitivity and excellent robustness against nonlinearities. However, conventional coherent receivers with complicated structure and heavy algorithms are too expensive and power-hungry for cost-sensitive MFN applications. In addition, currently deployed digital MFNs based on common public radio interface (CPRI) suffer from low spectral efficiency and high data rate. Towards these issues we propose a novel DRoF downlink scheme employing a simple self-coherent receiver. In baseband unit (BBU), the radio signal is converted to a digital bit stream by a band-pass delta-sigma modulator (BP-DSM), which can be simply recovered with the utilization of a band-pass filter at the receiver. In remote radio unit (RRU), an electro-absorption modulated laser (EML) acts as a low-cost coherent homodyne receiver in virtue of injection locking technique. In the experiment, the injection-locked operation of the DSM signal is successfully achieved, and two modified schemes are proposed for the DSM signal to increase the locking range with a tolerable sensitivity penalty. The experimental results demonstrate the superiority of our approach in two aspects: 1) the EML-based coherent receiver outperforms a PIN photodiode in terms of receiver sensitivity; 2) compared to the analog RoF system, a 5-dB improvement in loss budget is obtained when DSM is employed with the aid of a simple equalizer.

Epigenetic regulation of the DNMT1/MT1G/KLF4/CA9 axis synergises the anticancer effects of sorafenib in hepatocellular carcinoma.

Sorafenib, a multikinase inhibitor, has been widely used as a first-line anticancer drug for advanced hepatocellular carcinoma (HCC). However, the development of drug resistance to sorafenib is frequently observed in clinical applications. Potential nonkinase targets of sorafenib have not been well documented and may provide insights into reversing drug resistance and enhancing drug efficacy. Herein, we report that sorafenib exerts its anticancer effects by activating metallothionein 1 G (MT1G) expression. MT1G is a novel marker in HCC that correlates well with patient survival. MT1G overexpression suppressed the cellular proliferation, migration, invasion, and tumour formation of HCC and sensitised cells to sorafenib treatment. However, the disruption of MT1G attenuated the anticancer effects of sorafenib. Mechanistically, sorafenib upregulated MT1G expression via hypomethylation of its promoter region by binding and inhibiting DNA methyltransferase 1 (DNMT1) and increasing its promoter accessibility in HCC cells. Activation of MT1G also inhibited CA9 transcription through the suppression of HIF1A as mediated by KLF4. Our collective data revealed that sorafenib exerts its anticancer effects through epigenetic regulation of the DNMT1/MT1G/KLF4/CA9 axis in HCC and the activation of MT1G might constitute a strategy for enhancing the effect of sorafenib to suppress HCC cells.

The effect of orthokeratology treatment zone decentration on myopia progression.

BACKGROUND: This study aimed to compare the changes in the axial length (AL) in myopic children that wear centered and decentered orthokeratology (Ortho-K). METHODS: This retrospective study included 217 subjects who were treated with an Ortho-K lens for >12 months. The subjects were divided into three groups based on the magnitude of the Ortho-K lens treatment zone decentration: mildly, moderately, and severely decentered groups. Distance and direction of treatment zone decentration were calculated using software that was developed in-house. The AL changes in different groups were compared. RESULTS: Based on the distance of the treatment zone decentration, 65 children (65 eyes) were included in the mildly decentered group, 114 children (114 eyes) in the moderately decentered group, and 38 children (38 eyes) in the severely decentered group. The mean decentration distance in the three groups was 0.35 ± 0.11 mm, 0.71 ± 0.13 mm, and 1.21 ± 0.22 mm, respectively. The mean AL increase in the three groups after 12 months of Ortho-K lens wear was 0.24 ± 0.21 mm, 0.23 ± 0.18 mm, and 0.19 ± 0.20 mm, respectively. There were no significant differences in AL changes among the three groups. CONCLUSIONS: Ortho-K lens decentration is common in clinical practice. The AL change after Ortho-K lens wear was not significantly different in subjects with different magnitudes of Ortho-K lens decentration. Fitting the Ortho-K lens in the properly centered zone is recommended to ensure the safety of Ortho-K lens wear and to maintain visual quality.

Clinical effect of rigid gas permeable (RGP) contact lens in improving vision and controlling myopia progression of unilateral high myopic children.

BACKGROUND: The aim of this study was to explore the clinical effect of the RGP contact lens in improving vision and controlling myopia progression of the unilateral high myopic children. METHODS: In this retrospective analysis case series study, we analyzed the clinical data for 23 subjects with unilateral high myopic children under 6 years old, with the spherical equivalent refractive error of the myopic eye from -5.0 D to -12.0 D, who were enrolled from the outpatient clinic at Beijing Tongren Hospital, China. Fourteen subjects could successfully fit the RGP contact lens, and nine subjects used the spectacle lens. Complete ocular examination was performed at the first visit at the clinic and every three months during the follow-up period. RESULTS: In the RGP group, the change of axial length (AL) of the high myopic eye was from 25.62 ± 1.31 mm to 25.69 ± 1.31 mm after 12 months. The change of spherical equivalent (SE) was from -8.73 ± 2.54 D to -8.48 ± 2.77 D. The best corrected visual acuity (BCVA) improved from 0.24 ± 0.18 to 0.78 ± 0.26. In the spectacle group, the change of AL was from 24.76 ± 0.56 mm to 24.94 ± 0.71 mm. The change of SE was from -6.16 ± 2.86 D to -9.45 ± 2.06 D. The improvement of BCVA was from 0.52 ± 0.15 to 0.72 ± 0.23. CONCLUSIONS: Wearing RGP lens in the children with unilateral high myopia is a safe, convenient and effective method to improve the best corrected vision and controlling myopia progression.

Demonstration of a 50G-PON with a 45-dB power budget using an IQ-interleaved coherent detection scheme.

The application of traditional coherent detection technology to optical access networks has been undermined due to its high complexity and high cost. In this paper, we propose a novel IQ-interleaved detection method which uses the preset frequency offset of the lasers at the transmitter and receiver to obtain the in-phase and quadrature components of the received signal. It keeps the simple structure of heterodyne detection and avoids the down-conversion process. Without Nyquist pulse shaping, the received signal bandwidth of the proposed scheme is theoretically 0.5B smaller than that of heterodyne detection for signal with a symbol rate of B. The 50-Gb/s NRZ transmission experiment proves that by using the proposed scheme, the receiving sensitivity and the frequency drift tolerance can be improved by ∼1 dB and 1 GHz compared with heterodyne detection under strong bandwidth limitation. Without pulse shaping, the receiving sensitivity, frequency drift tolerance (1-dB sensitivity penalty) and link power budget for 20-km fiber transmission are -31.8 dBm, 11 GHz and 43.5 dB, respectively. A higher power budget of 45 dB can be achieved when Nyquist pulse shaping is applied. The proposed scheme provides a low-complexity potential solution for a next-generation coherent PON.

Design of a dual-signal sensing platform for d-penicillamine based on UiO-66-NH2 MOFs and APBA@Alizarin Red.

Herein, we designed a diversified sensing platform for d-penicillamine based on amino-functionalized Zr-based metal-organic frameworks (UiO-66-NH2 MOFs) and 3-aminophenylboronic acid (APBA)@Alizarin Red (ARS). The boronic acid group of 3-aminophenylboronic acid could react with Alizarin Red to form an APBA@ARS complex with a yellow fluorescence emission at 580 nm and ultraviolet absorption at 435 nm. APBA@ARS can greatly quench the fluorescence of UiO-66-NH2 MOFs at 450 nm via fluorescence resonance energy transfer (FRET). When copper ions were present in the reaction system of APBA and Alizarin Red, the copper ions could complex with Alizarin Red to prevent the generation of APBA@ARS, and the absorption of Cu@ARS at 530 nm occurred. Thus, the absorbance of APBA@ARS at 435 nm declined, restoring the fluorescence of UiO-66-NH2 MOFs. Nevertheless, when d-penicillamine and copper ions coexist in the APBA and Alizarin Red reaction system, the copper ions would complex with the sulfhydryl group of d-penicillamine and no longer hinder the generation of APBA@ARS, and the fluorescence of UiO-66-NH2 MOFs is quenched again. Meanwhile, the absorbance of APBA@ARS at 435 nm enhanced and the absorbance at 530 nm decreased. Thus, a fluorescence and colorimetric dual-signal sensing platform was constructed for d-penicillamine detection, which could detect d-penicillamine in the 1-20 µM and 2-50 µM ranges with the limit of detection (LOD) values of 0.46 µM and 1.38 µM, respectively. Furthermore, this sensing platform could also realize the intelligent RGB detection via mobile phones due to the obvious color change of the reaction system.

Demonstration of symmetrical 50-Gb/s TDM-PON in O-band supporting over 33-dB link budget with OLT-side amplification.

Single wavelength 50 Gb/s passive optical network (PON) is an excellent candidate for meeting high capacity requirements. In this paper, we experimentally investigate a symmetrical 50 Gb/s time division multiplexed passive optical network (TDM-PON) system in the O-band based on 25G optics. Semiconductor optical amplifier (SOA) is used in optical line terminal (OLT) side to improve link power budget. We initially investigate the performances of SOA as a booster amplifier with different gain in the downstream and make a trade-off between receiver sensitivity and power budget. The performances of 50 Gb/s non-return-to-zero (NRZ) in the downstream with avalanche photodiode (APD) receiver and upstream with SOA-PIN receiver with different equalization schemes are evaluated. Experimental results show that up to 34.97 dB link power budget is achieved in the downstream direction with 7-tap feed forward equalization (FFE), and 33.76 dB link power budget is achieved in the upstream direction with only 3-tap FFE filtering.

Comprehensive comparison of multiple quantitative near-infrared spectroscopy models for Aspergillus flavus contamination detection in peanut.

BACKGROUND: Aspergillus flavus is a major pollutant in moldy peanuts, and it has a large influence on the taste of food. The secondary metabolites of Aspergillus flavus, including aflatoxin B1 (AFB1) and aflatoxin B2 (AFB2), are highly toxic and can expose humans to high risk. The total mold count (TMC) is an important index to determine the contamination degree and hygiene quality of peanut. RESULTS: Quantitative calibration models were established based on full-band wavelengths and characteristic wavelengths, combined with chemometric methods, to explore the feasibility of the use of near-infrared spectroscopy (NIRS) for rapid detection of the TMC in peanuts. The successive projection algorithm (SPA) and elimination of uninformative variables (UVE) algorithms were used to extract the characteristic wavelengths. In comparison, the model built by original spectrum, selected with the UVE algorithm, gave the best result, with a correlation coefficient in a prediction set (RP ) of 0.9577, a root mean square error for the prediction set (RMSEP) of 0.2336 Log CFU/g, and a residual predictive deviation (RPD) of 3.5041. CONCLUSIONS: The results showed that NIRS is a rapid, practicable method for the quantitative detection of peanut Aspergillus flavus contamination. It is a promising method for detecting moldy peanuts and increasing peanut safety. © 2019 Society of Chemical Industry.

Investigation on the equalization techniques for 10G-class optics enabled 25G-EPON.

High speed modulation based on bandwidth limited devices is desired for cost-effective PON capacity upgrade. In this paper, we investigate the equalization techniques for enabling 25-Gb/s transmission with 10G-class optics. A comparison between FFE and DFE based equalizer and MLSE based digital equalizer is made, where 13-tap FFE and 3-tap DFE are required to obtain similar performances with MLSE based detection. In addition, to verify the cost introduced by the ADC, the demand for the ADC parameters in the MLSE based detector, including the sampling rate, resolution, and timing jitter is investigated. Experimental results show that using a 25-GS/s ADC with 4-bit resolution, 25-Gb/s transmission is realized using 10-G TOSA and ROSA, and 28-/30-dB loss budget can be achieved in C-/O-band respectively.

100-Gb/s TWDM-PON based on 10G optical devices.

High speed data modulation based on bandwidth limited devices has been considered as a cost-effective way to upgrade 10G-EPON to the next generation 100G-EPON. In this paper, we experimentally demonstrate the modulation, fiber transmission and reception of 25-Gb/s signal based on directly modulated laser and photo-detector both operating at 10 GHz. Instead of digital signal processing, the chirp management, dispersion compensation and frequency equalization in our scheme are realized in optical domain using a single delay interferometer. Three popular formats are investigated, including NRZ-OOK, PAM-4 and duobinary. According to the experimental results, the NRZ-OOK format shows its superiority in both launch power and receiver sensitivity, which provides a cost-effective solution for the construction of 100-Gb/s TWDM-PON.

28 Gb/s duobinary signal transmission over 40 km based on 10 GHz DML and PIN for 100 Gb/s PON.

In this paper, we demonstrate the direct modulation and direct detection of 28-Gb/s duobinary signal for the future downstream capacity upgrade in next generation passive optical network (PON). Commercial 10-GHz directly modulated laser (DML) and PIN with a combined modulation bandwidth of ~7 GHz are used as transmitter and receiver respectively. In order to mitigate the chromatic dispersion induced signal distortion, an optical delay interferometer (DI) is employed to narrow down the signal spectrum, thereby realizing 40-km single mode fiber (SMF) transmission in C-band. Besides, the chirp-induced spectral broadening of the directly modulated signal enables a higher launch power than external modulation schemes, which increases the loss budget of the system. As a result, 31-dB loss budget is achieved, supporting 64 users with 40-km reach. Also, as the transceivers in both optical line terminal (OLT) and optical network unit (ONU) are commercial l0-GHz devices, the proposed scheme is compatible with 40-Gb/s time and wavelength division multiplexing passive optical network (TWDM-PON) systems, providing a cost-efficient alternative for the development of 100G PON.

Upstream dispersion management supporting 100 km differential reach in TWDM-PON.

An optical dispersion compensator (ODC) with negative dispersion value in optical line terminal (OLT) is proposed to manage the chromatic dispersion of 10 Gb/s upstream directly-modulated signals from users with 100 km differential distances, achieving a maximal 51.9 dB loss budget thanks to the positive chirp of the directly-modulated signals and the characteristics of access networks that lower loss budget is required for the users at shorter transmission distance. The optimal dispersion value of the ODC is determined by the maximal distance of the users and the objective is to guarantee the loss budget monotonically improved with the increase of reach, therefore supporting differential reach. Experimental results show that it is a potential solution for practical implementation of long-reach and high splitting-ratio time and wavelength division multiplexed passive optical network (TWDM-PON).

Symmetric 40-Gb/s TWDM-PON with 51-dB loss budget by using a single SOA as preamplifier, booster and format converter in ONU.

In this paper, we propose to use a semiconductor optical amplifier (SOA) in the optical network unit (ONU) to improve the loss budget in time and wavelength division multiplexed-passive optical network (TWDM-PON) systems. The SOA boosts the upstream signal to increase the output power of the electro-absorption modulated laser (EML) and simultaneously pre-amplifies the downstream signal for sensitivity improvement. The penalty caused by cross gain modulation (XGM) effect is negligible due to the low extinction ratio (ER) of upstream signal and the large wavelength difference between upstream and downstream links. In order to achieve a higher output power, the SOA is driven into its saturation region, where the self-phase modulation (SPM) effect converts the intensity into phase information and realizes on-off-keying (OOK) to phase-shifted-keying (PSK) format conversion. In this way, the pattern effect is eliminated, which releases the requirement of gain-clamping on SOA. To further improve the loss budget of upstream link, an Erbium doped fiber amplifier (EDFA) is used in the optical line terminal (OLT) to pre-amplify the received signal. For the downstream direction, directly modulated laser (DML) is used as the laser source. Taking advantage of its carrier-less characteristic, directly modulated signal shows high tolerance to fiber nonlinearity, which could support a downstream launch power as high as + 16 dBm per channel. In addition, the signal is pre-amplified by the SOA in ONU before being detected, so the sensitivity limitation for downstream link is also removed. As a result, a truly passive symmetric 40-Gb/s TWDM-PON was demonstrated, achieving a link loss budget of 51 dB.

A novel robust hydrogel-assisted paper-based sensor based on fluorescence UiO-66-NH2@ZIF-8 for the dual-channel detection of captopril.

Captopril (CP) is commonly used as an active enzyme inhibitor for the treatment of coronary heart disease, hypertension and angina pectoris. The development of sensitive and efficient method for CP analysis is of great importance in biomedical research. Herein, we fabricated a sensitive and robust hydrogel-assisted paper-based sensor based on fluorescence UiO-66-NH2@ZIF-8 and Co, N-doped carbon nanozymes with oxidase-mimicking activity for accurate monitoring of captopril. The hydrogel-assisted paper-based sensor appeared a visible pink signal due to the catalytic oxidation of colorless N,N-diethyl-p-phenylenediamine (DPD) to oxDPD by Co, N-doped carbon-based nanozymes, and resulted in the fluorescence quenching of UiO-66-NH2@ZIF-8. In the presence of captopril, the oxidation of chromogenic substrate DPD by Co, N-doped nanozymes in the hydrogel-assisted paper-based sensor was hindered and accompanied by a change in the visible color, leading to recovery of the fluorescence of UiO-66-NH2@ZIF-8, and the change in the fluorescence color could also be observed. Therefore, the quantitative detection of captopril is achieved by taking a smartphone photograph and converting the image parameters into data information using ImageJ software. The portable hydrogel-assisted paper sensor provided sensitive detection of captopril in two modes based on visible color change as well as fluorescence color change with limits of detection of 0.45 µM and 0.47 µM, respectively. This hydrogel-assisted paper-based sensor has been successfully applied to the accurate monitoring of captopril in human serum, providing a potential avenue for in situ detection of captopril.

Macrophage-Derived Nanosponges Adsorb Cytokines and Modulate Macrophage Polarization for Renal Cell Carcinoma Immunotherapy.

Renal cell carcinoma (RCC) is a hot tumor infiltrated by large numbers of CD8+ T cells and is highly sensitive to immunotherapy. However, tumor-associated macrophages (TAMs), mainly M2 macrophages, tend to undermine the efficacy of immunotherapy and promote the progression of RCC. Here, macrophage-derived nanosponges are fabricated by M2 macrophage membrane-coated poly（lactic-co-glycolic acid）（PLGA）, which could chemotaxis to the CXC and CC chemokine subfamily-enriched RCC microenvironment via corresponding membrane chemokine receptors. Subsequently, the nanosponges act like cytokine decoys to adsorb and neutralize broad-spectrum immunosuppressive cytokines such as colony stimulating factor-1(CSF-1), transforming growth factor-ß（TGF-ß）, and Lnterleukin-10（IL-10）, thereby reversing the polarization of M2-TAMs toward the pro-inflammatory M1 phenotype, and enhancing the anti-tumor effect of CD8+ T cells. To further enhance the polarization reprogramming efficiency of TAMs, DSPE-PEG-M2pep is conjugated on the surface of macrophage-derived nanosponges for specific recognition of M2-TAMs, and the toll like receptors 7/8（TLR7/8） agonist, R848, is encapsulated in these nanosponges to induce M1 polarization, which result in significant efficacy against RCC. In addition, these nanosponges exhibit undetectable biotoxicity, making them suitable for clinical applications. In summary, a promising and facile strategy is provided for immunomodulatory therapies, which are expected to be used in the treatment of tumors, autoimmune diseases, and inflammatory diseases.

Genetically Engineered Membrane-Coated Nanoparticles for Enhanced Prostate-Specific Membrane Antigen Targeting and Ferroptosis Treatment of Castration-Resistant Prostate Cancer.

Conventional androgen deprivation therapy (ADT) targets the androgen receptor (AR) inhibiting prostate cancer (PCa) progression; however, it can eventually lead to recurrence as castration-resistant PCa (CRPC), which has high mortality rates and lacks effective treatment modalities. The study confirms the presence of high glutathione peroxidase 4 (GPX4) expression, a key regulator of ferroptosis (i.e., iron-dependent program cell death) in CRPC cells. Therefore, inducing ferroptosis in CRPC cells might be an effective therapeutic modality for CRPC. However, nonspecific uptake of ferroptosis inducers can result in undesirable cytotoxicity in major organs. Thus, to precisely induce ferroptosis in CRPC cells, a genetic engineering strategy is proposed to embed a prostate-specific membrane antigen (PSMA)-targeting antibody fragment (gy1) in the macrophage membrane, which is then coated onto mesoporous polydopamine (MPDA) nanoparticles to produce a biomimetic nanoplatform. The results indicate that the membrane-coated nanoparticles (MNPs) exhibit high specificity and affinity toward CRPC cells. On further encapsulation with the ferroptosis inducers RSL3 and iron ions, MPDA/Fe/RSL3@M-gy1 demonstrates superior synergistic effects in highly targeted ferroptosis therapy eliciting significant therapeutic efficacy against CRPC tumor growth and bone metastasis without increased cytotoxicity. In conclusion, a new therapeutic strategy is reported for the PSMA-specific, CRPC-targeting platform for ferroptosis induction with increased efficacy and safety.

Simultaneous DPSK demodulation and chirp management using delay interferometer in symmetric 40-Gb/s capability TWDM-PON system.

We propose a symmetric 40-Gb/s aggregate rate time and wavelength division multiplexed passive optical network (TWDM-PON) system with the capability of simultaneous downstream differential phase shift keying (DPSK) signal demodulation and upstream signal chirp management based on delay interferometer (DI). With the bi-pass characteristic of DI, we experimentally demonstrate the bidirectional transmission of signals at 10-Gb/s per wavelength, and achieve negligible power penalties after 50-km single mode fiber (SMF). For the uplink transmission with DI, a ~11-dB optical power budget improvement at a bit error ratio of 1e-3 is obtained and the extinction ratio (ER) of signal is also improved from 3.4 dB to 13.75 dB. Owing to this high ER, the upstream burst-mode transmitting is successfully presented in term of time-division multiplexing. Moreover, in our experiment, a ~38-dB power budget is obtained to support 256 users with 50-km SMF transmission.

A sensing platform for on-site detection of glutathione S-transferase using oxidized Pi@Ce-doped Zr-based metal-organic frameworks(MOFs).

The development of point-of-care testing (POCT) for glutathione S-transferase (GST) is an effective way to establish the mechanism of targeted monitoring of cancer chemotherapy drug metabolism. Assays for GST with high sensitivity as well as on-site screening have been urgently required to monitor this process. Herein, we synthesized oxidized Pi@Ce-doped Zr-based metal-organic frameworks (MOFs) by electrostatic self-assembly between phosphate and oxidized Ce-doped Zr-based MOFs. It was found that the oxidase-like activity of oxidized Pi@Ce-doped Zr-based MOFs was substantially increased after phosphate ion (Pi) assembly. And a stimulus-responsive hydrogel-based kit was constructed by embedding oxidized Pi@Ce-doped Zr-based MOFs into a PVA (polyvinyl alcohol) hydrogel system, we integrated a portable hydrogel kit with a smartphone for real-time monitoring of GST for quantitative and accurate analysis. The color reaction was triggered based on oxidized Pi@Ce-doped Zr-based MOFs with 3,3',5,5'-tetramethylbenzidine (TMB). However, in the presence of glutathione (GSH), the above color reaction was hindered due to the reducibility of GSH. Catalyzed by GST, GSH can react with 1-chloro-2,4-dinitrobenzo (CDNB) to form an adduct, which caused the color reaction to occur again, resulting in the color response of the kit. In combination with ImageJ software, the kit image information acquired by smartphone could be converted into hue intensity, providing a direct quantitative tool for the detection of GST with a detection limit of 0.19mU·L-1. Based on the advantages of simple operation and cost-effectiveness, the introduction of the POCT miniaturized biosensor platform will meet the requirements of on-site quantitative analysis of GST.