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1.
Cytokine ; 146: 155557, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34303273

RESUMO

AIM: Atrial fibrillation (AF) is a common clinical arrhythmia and can cause a variety of complications. To study the therapeutic effect of H2S in atrial fibrosis and explore the important role of miR-133a, in vitro experiments in human atrial fibroblasts (HAFs) were conducted. METHODS: The fibrosis in HAFs was induced by Ang II. The expression levels of miR-133a and CTGF in HAFs were examined by qRT-PCR. The proliferation and migration of HAFs were detected by CCK-8 and cell scratch assays. The protein expressions of CTGF, collagen I, collagen III and α-SMA were detected by western blotting. The dual-luciferase reporter gene was used to detect the interaction between miR-133a and CTGF. RESULTS: The proliferation and migration of HAFs stimulated by Ang II were enhanced, the expression of miR-133a was reduced, and the levels of CTGF and fibrosis markers (collagen I, collagen III and α-SMA) were increased. Furthermore, H2S reduced fibrosis, proliferation and migration of HAFs induced by Ang II. Accordingly, overexpression of miR-133a inhibited the proliferation and migration ability on Ang II-induced HAFs, and decreased the protein expressions of related fibrosis markers and CTGF. Meanwhile, miR-133a inhibitor could reverse the inhibition effect of H2S on proliferation and migration in HAFs by Ang II-induced. By targeting CTGF, miR-133a inhibited the expression of CTGF. CONCLUSION: H2S improved myocardial cell fibrosis by significantly increasing the expression of miR-133a, and CTGF might be a potential target for miR-133a to play an important role in myocardial fibrosis.


Assuntos
Fibrilação Atrial/tratamento farmacológico , Fibrilação Atrial/genética , Fator de Crescimento do Tecido Conjuntivo/metabolismo , Átrios do Coração/patologia , Sulfeto de Hidrogênio/uso terapêutico , MicroRNAs/metabolismo , Angiotensina II , Sequência de Bases , Fibroblastos/efeitos dos fármacos , Fibroblastos/metabolismo , Fibroblastos/patologia , Fibrose , Humanos , Sulfeto de Hidrogênio/farmacologia , MicroRNAs/genética
2.
Biochem Biophys Res Commun ; 521(2): 485-491, 2020 01 08.
Artigo em Inglês | MEDLINE | ID: mdl-31677784

RESUMO

Oxidative stress and cardiomyocyte apoptosis contributed to the progression of doxorubicin (Dox)-induced cardiotoxicity. Recent studies identified microRNA-22 (miR-22) as a cardiac- and skeletal muscle-enriched microRNA that functioned as a key regulator in stress-induced cardiac injury. The present study aimed to investigate the role and possible mechanism of miR-22 on Dox-induced oxidative stress and cardiomyocyte apoptosis. Mice were exposed to reduplicative injections of Dox (i.p., 4 mg/kg) weekly for consecutive 4 weeks to generate Dox-induced cardiotoxicity. Herein, we found that miR-22 level was significantly increased in murine hearts subjected to chronic Dox treatment. MiR-22 inhibition attenuated oxidative stress and cardiomyocyte apoptosis in vivo and in vitro, thereby preventing Dox-induced cardiac dysfunction. Mechanistically, we observed that miR-22 directly bound to the 3'-UTR of Sirt1 and caused SIRT1 downregulation. Conversely, miR-22 antagomir upregulated SIRT1 expression and SIRT1 inhibitor abolished the beneficial effects of miR-22 antagomir. In conclusion, miR-22 inhibition prevented oxidative stress and cardiomyocyte apoptosis via upregulating SIRT1 and miR-22 might be a new target for treating Dox-induced cardiotoxicity.


Assuntos
Cardiotoxicidade/prevenção & controle , Doxorrubicina/efeitos adversos , MicroRNAs/antagonistas & inibidores , Sirtuína 1/metabolismo , Regiões 3' não Traduzidas , Animais , Antagomirs/farmacologia , Apoptose/efeitos dos fármacos , Cardiotoxicidade/etiologia , Camundongos , MicroRNAs/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Sirtuína 1/genética , Regulação para Cima
3.
Cardiology ; 141(4): 226-232, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30852569

RESUMO

BACKGROUND: Postinfarction ventricular septal rupture (PI-VSR) is a rare but devastating complication of acute myocardial infarction (AMI). Risk stratification in the acute phase is crucial for decision-making, and this study analyzed the risk factors for early mortality and the effects of various management options on the outcome of PI-VSR patients in the era of percutaneous intervention. METHODS: A total of 96 patients with PI-VSR were identified and divided into an acute-phase survivor group (n = 46, survived ≥2 weeks after admission) and a nonsurvivor group (n = 50, died within 2 weeks after admission). Percutaneous closure was considered in acute-phase survivors. Patients were followed up for a mean 47 (quartiles 15-71) months by clinical visit or telephone interview. RESULTS: The overall acute-phase (i.e., < 2 weeks after the diagnosis of PI-VSR) mortality rate was 52%. Female sex and Killip Class III-IV at admission were associated with an increased risk of acute-phase death. Of the 46 patients who survived ≥2 weeks, 20 underwent interventional occlusion and the procedure was successful in 19. Percutaneous closure in the acute-phase survivor group improved the immediate (21% in-hospital mortality rate) and long-term (53% mortality) outcomes. CONCLUSIONS: Patients with PI-VSR are at a high risk of acute-phase mortality. Female sex and severe cardiac dysfunction at admission are linked with a high rate of acute-phase deaths. Percutaneous closure in acute-phase survivors results in favorable short- and long-term benefits for PI-VSR patients.


Assuntos
Cateterismo Cardíaco/métodos , Procedimentos Cirúrgicos Cardíacos/métodos , Infarto do Miocárdio/complicações , Ruptura do Septo Ventricular/cirurgia , Idoso , China , Feminino , Mortalidade Hospitalar , Humanos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Infarto do Miocárdio/mortalidade , Estudos Retrospectivos , Medição de Risco , Fatores de Risco , Dispositivo para Oclusão Septal , Ruptura do Septo Ventricular/etiologia , Ruptura do Septo Ventricular/mortalidade
4.
Biochem Biophys Res Commun ; 447(2): 271-7, 2014 May 02.
Artigo em Inglês | MEDLINE | ID: mdl-24704450

RESUMO

The molecular mechanisms of multiple myeloma are not well defined. EEN is an endocytosis-regulating molecule. Here we report that EEN regulates the proliferation and survival of multiple myeloma cells, by regulating IGF-1 secretion. In the present study, we observed that EEN expression paralleled with cell proliferation, EEN accelerated cell proliferation, facilitated cell cycle transition from G1 to S phase by regulating cyclin-dependent kinases (CDKs) pathway, and delayed cell apoptosis via Bcl2/Bax-mitochondrial pathway. Mechanistically, we found that EEN was indispensable for insulin-like growth factor-1 (IGF-1) secretion and the activation of protein kinase B-mammalian target of rapamycin (Akt-mTOR) pathway. Exogenous IGF-1 overcame the phenotype of EEN depletion, while IGF-1 neutralization overcame that of EEN over-expression. Collectively, these data suggest that EEN may play a pivotal role in excessive cell proliferation and insufficient cell apoptosis of bone marrow plasma cells in multiple myeloma. Therefore, EEN may represent a potential diagnostic marker or therapeutic target for multiple myeloma.


Assuntos
Biomarcadores Tumorais/fisiologia , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Mieloma Múltiplo/patologia , Receptor IGF Tipo 1/metabolismo , Apoptose , Biomarcadores Tumorais/genética , Linhagem Celular Tumoral , Proliferação de Células , Sobrevivência Celular , Pontos de Checagem da Fase G1 do Ciclo Celular , Humanos , Peptídeos e Proteínas de Sinalização Intracelular/genética , Mieloma Múltiplo/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Receptor IGF Tipo 1/farmacologia , Serina-Treonina Quinases TOR/metabolismo
5.
Nat Chem ; 16(9): 1505-1514, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-38844635

RESUMO

Halogenated organic pollutants (HOPs) are causing a significant environmental and human health crisis due to their high levels of toxicity, persistence and bioaccumulation. Urgent action is required to develop effective approaches for the reduction and reuse of HOPs. Whereas current strategies focus primarily on the degradation of HOPs, repurposing them is an alternative approach, albeit a challenging task. Here we discover that alkyl bromide can act as a catalyst for the transfer of chlorine using alkyl chloride as the chlorine source. We demonstrate that this approach has a wide substrate scope, and we successfully apply it to reuse HOPs that include dichlorodiphenyltrichloroethane, hexabromocyclododecane, chlorinated paraffins, chloromethyl polystyrene and poly(vinyl chloride) (PVC). Moreover, we show that the synthesis of essential non-steroidal anti-inflammatory drugs can be achieved using PVC and hexabromocyclododecane, and we demonstrate that PVC waste can be used directly as a chlorinating agent. Overall, this methodology offers a promising strategy for repurposing HOPs.

6.
Fa Yi Xue Za Zhi ; 29(5): 348-52, 2013 Oct.
Artigo em Zh | MEDLINE | ID: mdl-24466774

RESUMO

OBJECTIVE: To analyze the variations of glycerol-3-phosphate dehydrogenase 1 like gene (GPD1-L) and address the association with sudden manhood death syndrome (SMDS). METHODS: The genomic DNA was extracted from blood samples of the SMDS group and the normal control group. The exons, exon-intron boundaries and 3'-UTRs of coding region of GPD1-L were PCR amplified and DNA sequenced directly to confirm the types of variations. The genotype frequency and allele frequency were analyzed statistically. RESULTS: There were two variants in the SMDS group, c.465C>T and c.*18G>T, the latter existed certain degree difference of genotype distribution and allele frequency between the SMDS group and the control group, but there was no statistically significant (P > 0.05). CONCLUSION: The relation between gene mutation of GPD1-L and the occurrence of Chinese SMDS deserves a further research.


Assuntos
Morte Súbita/etiologia , Glicerolfosfato Desidrogenase/genética , Mutação , Adolescente , Adulto , Povo Asiático/genética , Sequência de Bases , Estudos de Casos e Controles , Análise Mutacional de DNA , Primers do DNA/genética , Éxons , Frequência do Gene , Genótipo , Glicerolfosfato Desidrogenase/sangue , Humanos , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Polimorfismo de Nucleotídeo Único , Adulto Jovem
7.
Adv Sci (Weinh) ; 10(31): e2304672, 2023 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-37632714

RESUMO

Metallocenes are privileged backbones in the fields of synthetic chemistry, catalysis, polymer science, etc. Direct C-H functionalization is undoubtedly the simplest approach for tuning the properties of metallocenes. However, owing to the presence of multiple identical C(sp2 )-H sites, this protocol often suffers from low reactivity and selectivity issues, especially for the regioselective synthesis of 1,3-difunctionalized metallocenes. Herein, an efficient iridium-catalyzed meta-selective C-H borylation of metallocenes is reported. With no need of preinstalled directing groups, this approach enables a rapid synthesis of various boronic esters based on benzoferrocenes, ferrocenes, ruthenocene, and related half sandwich complex. A broad range of electron-deficient and -rich functional groups are all compatible with the process. Notably, C-H borylation of benzoferrocenes takes place exclusively at the benzene ring, which is likely ascribed to the shielding effect of pentamethylcyclopentadiene. The synthetic utility is further demonstrated by easy scalability to gram quantities, the conversion of boron to heteroatoms including N3 , SePh, and OAc, as well as diverse cross-coupling reactions.

8.
Exp Ther Med ; 26(1): 320, 2023 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-37273757

RESUMO

Pachymic acid (Pac), a major bioactive constituent of Poria cocos, is an antioxidant that inhibits triglyceride (TG) accumulation. To the best of our knowledge, the present study investigated for the first time whether Pac activated sirtuin 6 (SIRT6) signaling to alleviate oleic acid (OA)-palmitic acid (PA)-induced lipid metabolism disorders in mouse primary hepatocytes (MPHs). In the present study, MPHs challenged with Pac were used to test the effects of Pac on intracellular lipid metabolism. Molecular docking studies were performed to explore the potential targets of Pac in defending against lipid deposition. MPHs isolated from liver-specific SIRT6-deficient mice were subjected to OA + PA incubation and treated with Pac to determine the function and detailed mechanism. It was revealed that Pac activated SIRT6 by increasing its expression and deacetylase activity. Pa prevented OA + PA-induced lipid deposition in MPHs in a dose-dependent manner. Pac (50 µM) administration significantly reduced TG accumulation and increased fatty acid oxidation rate in OA + PA-incubated MPHs. Meanwhile, as per the results of molecular docking and relative mRNA levels, Pac activated SIRT6 and increased SIRT6 deacetylation levels. Furthermore, SIRT6 deletions in MPHs abolished the protective effects of Pac against OA + PA-induced hepatocyte lipid metabolism disorders. The present study demonstrated that Pac alleviates OA + PA-induced hepatocyte lipid metabolism disorders by activating SIRT6 signaling. Overall, SIRT6 signaling increases oxidative stress burden and promotes hepatocyte lipolysis.

9.
Zhong Yao Cai ; 35(6): 930-5, 2012 Jun.
Artigo em Zh | MEDLINE | ID: mdl-23236829

RESUMO

OBJECTIVE: To study the anti-portal hypertension effect of oleanolic acid (OA) in CCl4-induced cirrhosis rats and its mechanism. METHODS: Rats were induced to portal hypertension by CCl4. After treatment with low dose of OA (30 mg/kg) and high dose of OA (60 mg/kg) by intragastrically for a month, the parameters in serum or liver tissue including ALT, AST, MDA, GSH-Px, NOx, eNOS, cGMP and type I collagen were measured. The MAP, PP and HR were determined by hameodynamic method and the eNOS expression in liver was measured by western blot. The pathological changes of liver tissue were also tested by Masson dye. The normal group and model group were given 0.25% of CMC-Na solution. RESULTS: Compared with the model group, treatment with 30 mg/kg and 60 mg/kg OA significantly decreased the levels of ALT, AST, ALP, gamma-GT and MDA and enhanced the level of GSH-Px in liver (P<0.05). Moreover, the collagen content also notably lowered in CCl4-induced cirrhosis rats, thus decreasing the portal pressure (PP). However, the MAP and HR were not affected by OA treatment. In addition, the expression of eNOS in liver markedly increased after one mouth treatment of OA, hereof enhancing the level of cGMP and NOx in the CCl4-induced portal hypertensive rats (P<0.05). CONCLUSION: OA could inhibit the progress of fibrosis and lower the PP in CCl4-induced portal hypertensive rats and the anti-portal hypertension effect might be related to increasing the expression of eNOS and enhance the NOx level in liver.


Assuntos
Hipertensão Portal/tratamento farmacológico , Cirrose Hepática Experimental/tratamento farmacológico , Óxido Nítrico Sintase Tipo III/metabolismo , Ácido Oleanólico/uso terapêutico , Fitoterapia , Substâncias Protetoras/uso terapêutico , Animais , Peso Corporal , Tetracloreto de Carbono/efeitos adversos , Modelos Animais de Doenças , Hipertensão Portal/etiologia , Hipertensão Portal/metabolismo , Fígado/efeitos dos fármacos , Fígado/metabolismo , Fígado/patologia , Cirrose Hepática Experimental/induzido quimicamente , Cirrose Hepática Experimental/metabolismo , Testes de Função Hepática , Masculino , Malondialdeído/metabolismo , Óxido Nítrico/metabolismo , Óxido Nítrico Sintase Tipo III/genética , Ácido Oleanólico/farmacologia , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Substâncias Protetoras/farmacologia , Ratos , Ratos Sprague-Dawley
10.
Fa Yi Xue Za Zhi ; 28(6): 451-5, 2012 Dec.
Artigo em Zh | MEDLINE | ID: mdl-23484330

RESUMO

OBJECTIVE: To investigate the genetic polymorphisms of 18 STR loci (D18S51, D21S11, D3S1358, FGA, D8S1179, upsilonWA, CSF1PO, D16S539, D7S820, D13S317, D5S818, D2S1338, D19S433, D12S391, TPOX, TH01, Penta E and D6S1043) in unrelated Uygur individuals in Kashi prefecture of Xinjiang and to explore the application value in forensic practice. METHODS: Blood samples from 1 381 unrelated Uygur individuals were amplified by using DNA Typer 15 Plus kit. The amplified products were detected by using 3130XL Genetic Analyzer and the genotyping was done by using GeneMapper ID v3.2. Population genetics parameters were calculated and compared with that of the other population. The genetic distance of Reynold's was calculated and phylogenetic tree was constructed at last. RESULTS: Of the 1 381 unrelated Uygur individuals, 231 alleles were detected, with an allele frequency of 0.0004-0.5304. The H values were 0.644-0.923, PIC values were 0.587-0.918, and DP values were 0.817-0.988, respectively, with a CPE > 0.9999999. The genetic distance was the longest (0.088 3) to Guangzhou Han population and the closest (0.0503) to Greek. CONCLUSION: The 18 STR loci in the Uygur population of Kashi prefecture of Xinjiang have high genetic polymorphisms which are close to Europeans, and can be satisfied as genetic markers of population individual identification and paternity testing.


Assuntos
Frequência do Gene/genética , Genética Populacional , Repetições de Microssatélites/genética , Polimorfismo Genético , Povo Asiático/genética , China/etnologia , Genética Forense/métodos , Loci Gênicos , Genótipo , Humanos , Reação em Cadeia da Polimerase
11.
Leg Med (Tokyo) ; 54: 101987, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34768042

RESUMO

In kinship tests, the investigating of the forensic STRs usually provides decisive information to resolve relationship cases. We describe a parentage case with 3 genetic incompatibilities (D6S1043, D18S51 and D2S1338) between the child and alleged parent. With 90 STR loci and 100 SNP loci, the massively parallel sequencing (MPS)-based genotyping results support the certainty of parentage, and the mismatched alleles were considered to be mutations. MPS can provide additional allele sequence structures that can be used to infer the origins of the mutations. SNPs as supplementary markers can provide effective information to give an unequivocal statement of the parentage.


Assuntos
Impressões Digitais de DNA , Polimorfismo de Nucleotídeo Único , Criança , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Repetições de Microssatélites/genética , Polimorfismo de Nucleotídeo Único/genética , Análise de Sequência de DNA
12.
Front Cardiovasc Med ; 9: 851214, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35433881

RESUMO

Background: This study aimed to investigate the impact of the COVID-19 pandemic on ST-segment elevation myocardial infarction (STEMI) care in China. Methods: We conducted a multicenter, retrospective cohort study in Hunan province (adjacent to the epidemic center), China. Consecutive patients presenting with STEMI within 12 h of symptom onset and receiving primary percutaneous coronary intervention, pharmaco-invasive strategy and only thrombolytic treatment, were enrolled from January 23, 2020 to April 8, 2020 (COVID-19 era group). The same data were also collected for the equivalent period of 2019 (pre-COVID-19 era group). Results: A total of 610 patients with STEMI (COVID-19 era group n = 286, pre-COVID-19 era group n = 324) were included. There was a decline in the number of STEMI admissions by 10.5% and STEMI-related PCI procedures by 12.7% in 2020 compared with the equivalent period of 2019. The key time intervals including time from symptom onset to first medical contact, symptom onset to door, door-to-balloon, symptom onset to balloon and symptom onset to thrombolysis showed no significant difference between these two groups. There were no significant differences for in-hospital death and major adverse cardiovascular events between these two groups. Conclusion: During the COVID-19 pandemic outbreak in China, we observed a decline in the number of STEMI admissions and STEMI-related PCI procedures. However, the key quality indicators of STEMI care were not significantly affected. Restructuring health services during the COVID-19 pandemic has not significantly adversely influenced the in-hospital outcomes.

13.
Nat Commun ; 13(1): 3496, 2022 Jun 17.
Artigo em Inglês | MEDLINE | ID: mdl-35715392

RESUMO

Metallocenes are privileged backbones for synthesis and catalysis. However, the direct dehydrogenative C-H functionalization of unsymmetric metallocenes suffers from reactivity and selectivity issues. Herein, we report an electrochemically driven regioselective C-H phosphorylation of group 8 metallocenes. Mechanistic investigations indicate this dehydrogenative cross coupling occurs through an electrophilic radical substitution of the metallocene with a phosphoryl radical, facilitated by the metallocene itself. This work not only offers an efficient and divergent synthesis of phosphorylated metallocenes, but also provides a guide to interpret the reactivity and regioselectivity for the C-H functionalization of unsymmetric metallocenes.

14.
Curr Eye Res ; 46(2): 232-238, 2021 02.
Artigo em Inglês | MEDLINE | ID: mdl-32757684

RESUMO

Purpose: This work aimed to investigate the influences of microRNA-340 (miR-340) on proliferation and apoptosis of retinoblastoma (RB) cells and explore its regulatory mechanism. MATERIALS AND METHODS: miR-340 mimic and inhibitor were applied for up-regulating or inhibiting the expression of miR-340 in RB cell lines. Then, CCK-8 and AnnexinV-FITC/PI staining were used to measure cell proliferation and apoptosis, respectively. After that, luciferase assay was performed to affirm the direct targets of miR-340. Furthermore, qRT-PCR and western blotting assay were carried out to detect the levels of miR-340 and KIF14. RESULTS: Our results indicated that the miR-340 was lowly expressed in RB cell lines, and up-regulation of miR-340 can decrease the proliferation and induce the apoptosis of RB cells. Moreover, we verified that miR-340 controls KIF14 expression, either directly or through a subsequent molecular cascade, and inversely related to its expression. The results obtained from the rescue assays presented that over-expression of KIF14 reversed the miR-340-mediated inhibition on malignant phenotype of RB cells. CONCLUSIONS: Overall, we proved that miR-340 can decrease the proliferation and increase the apoptosis of RB cells, and its function in RB cells was at least partially achieved via down-regulation of KIF14, prompting that miR-340 was expected to supply a new direction for clinical therapy of RB in the future.


Assuntos
Apoptose/genética , Regulação Neoplásica da Expressão Gênica , Cinesinas/genética , MicroRNAs/genética , Proteínas Oncogênicas/genética , Neoplasias da Retina/genética , Retinoblastoma/genética , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Humanos , Cinesinas/biossíntese , MicroRNAs/biossíntese , Proteínas Oncogênicas/biossíntese , RNA Neoplásico/genética , RNA Neoplásico/metabolismo , Neoplasias da Retina/metabolismo , Neoplasias da Retina/patologia , Retinoblastoma/metabolismo , Retinoblastoma/patologia
15.
Yi Chuan ; 31(9): 921-35, 2009 Sep.
Artigo em Zh | MEDLINE | ID: mdl-19819845

RESUMO

To investigate the genetic polymorphism of Y-chromosomal short tandem repeats (STR) loci in Jing, Yi, Yao, and Zhuang minority populations from Guangxi Province, China. 17 Y-STR loci were co-amplified using AmpFlSTR(R) Yfiler PCR Amplification Kit System, and the PCR products were analyzed by genetic analyzer. Cluster and phylogenic tree analyses were conducted to show the genetic distance among the populations. There were 61 different haplotypes in 100 unrelated Yao males, 67 in 105 unrelated Yi males, 79 in 103 unrelated Jing males, and 91 in 107 unrelated Zhuang males. The haplotype diversities of Jing, Yi, Yao and Zhuang were determined as 0.9784, 0.9866, 0.9911, and 0.9956, respectively. Among these 4 minority populations, the genetic distance between Jing and Zhuang was the smallest (0.0391), while the genetic distance between Yi and Yao was the largest (0.3376). The 17 Y-STR loci in the 4 minority populations from Guangxi Province revealed a highly polymorphic genetic distribution, which show a high potential for population genetics and forensic practice.


Assuntos
Cromossomos Humanos Y/genética , Polimorfismo Genético/genética , Sequências de Repetição em Tandem/genética , China , Feminino , Genética Populacional , Haplótipos , Humanos , Masculino , Grupos Minoritários/estatística & dados numéricos , Reação em Cadeia da Polimerase
16.
J Chromatogr B Analyt Technol Biomed Life Sci ; 862(1-2): 189-95, 2008 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-18164671

RESUMO

A sensitive method for the determination of CQP propionic acid in rat plasma was developed and validated after solid-phase extraction. Chromatographic separation was achieved on a reversed-phase Alltima C18 column with the mobile phase of methanol-0.15% (v/v) phosphoric acid solution (pH 2.5) and step gradient elution resulted in a total run time of about 20min. The analytes were detected by using UV detector at 345nm. A good linear relationship was obtained in the concentration range of 50-12,800ng/mL (r=0.9998). The intra-day RSDs and the inter-day RSDs at the concentration of 200, 800, 6400 and 12,800ng/mL were less than 7.0% and 11.0%, respectively. The intra-day accuracy ranged from 96.3 to 106.5% and the inter-day accuracy ranged from 98.6 to 113.4%, respectively. Average extraction recoveries ranged from 83.6 to 94.3% in plasma at the concentrations of 200, 800, 6400 and 12,800ng/mL. This method was successfully applied to the pharmacokinetic studies on rats.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Propionatos/sangue , Animais , Masculino , Propionatos/farmacocinética , Ratos , Ratos Sprague-Dawley , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Espectrofotometria Ultravioleta
17.
Chin Med J (Engl) ; 121(2): 112-7, 2008 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-18272035

RESUMO

BACKGROUND: Scavenger receptor that binds phosphatidylserine and oxidized lipoprotein/CXC chemokine ligand 16 (SR-PSOX/CXCL16) promotes foam cell formation through the tumor necrosis factor (TNF)-alpha mediated mechanism. Because chemokine CXCL16 could be expressed in atherosclerotic lesions and induce smooth muscle cell (SMC) proliferation, we presume that the monocyte SR-PSOX/CXCL16 detection in the patients' peripheral blood will be important for early diagnosis and prognosis of atherosclerosis (AS). METHODS: Enrolled in this study were 40 patients with acute coronary syndrome (ACS), including 20 patients with acute myocardial infarction (AMI) and 20 patients with unstable angina pectoris (UAP), and 20 normal controls. Monocytes in the peripheral blood were isolated, and the changes of expression of CXCL16/SR-PSOX mRNA were compared using reverse transcription-polymerase chain reaction (RT-PCR), with beta-actin as internal control. We compared the expression of CXCL16/SR-PSOX in the ACS subgroups, using Western-blot to analyze protein expression levels. Tissue sections were made from biopsy specimens taken from patients with infective endocarditis, liver cirrhosis, and lung cancer as well as normal controls. And the expression of CXCL16/SR-PSOX was analyzed with a confocal microscope. RESULTS: The expression of CXCL16/SR-PSOX mRNA and protein in the monocytes of peripheral blood was significantly higher in ACS patients than in normal controls (P < 0.05); however, there was no significant difference in CXCL16/SR-PSOX expression between UAP group and AMI group (P > 0.05). Immunofluorescence showed that there were low expression of SR-PSOX in normal vascular endothelial cells and enhanced expression in every layer of the infected vessels, while spreading from endothelial cells to surrounding tissues as infection worsens. Confocal microscopy showed that the expression of SR-PSOX was enhanced in the infiltrated lymphocytes in liver cirrhosis, and that the expression level was proportionate to the degree of inflammation in the portal hepatis and folia. CONCLUSIONS: The expression of CXCL16/SR-PSOX in the monocytes of peripheral blood was significantly higher in ACS patients than in the controls. CXCL16/SR-PSOX-mediated inflammation may contribute to the pathogenesis of ACS, and CXCL16 may play an important role in the pathogenesis and development of AS in humans.


Assuntos
Síndrome Coronariana Aguda/imunologia , Quimiocinas CXC/sangue , Receptores Depuradores/sangue , Western Blotting , Quimiocina CXCL16 , Quimiocinas CXC/genética , Angiografia Coronária , Imunofluorescência , Humanos , RNA Mensageiro/sangue , Receptores Depuradores/genética
18.
J Chromatogr Sci ; 46(5): 419-23, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-18492352

RESUMO

The metabolism of borneol is studied by the analysis of incubations of in vitro-prepared rat liver microsomes. A sensitive gas chromatography (GC)-mass spectrometry (MS) method is developed for the identification of borneol and its metabolites. Four novel metabolites, which have not previously been reported, are isolated and confirmed by comparison of the GC-MS method. The biotransformation pathway of borneol in rat liver microsomes is proposed based on the in vitro results.


Assuntos
Canfanos/metabolismo , Cromatografia Gasosa-Espectrometria de Massas/métodos , Microssomos Hepáticos/metabolismo , Animais , Técnicas In Vitro , Masculino , Ratos , Ratos Sprague-Dawley
19.
Fa Yi Xue Za Zhi ; 24(6): 423-4, 428, 2008 Dec.
Artigo em Zh | MEDLINE | ID: mdl-19241967

RESUMO

OBJECTIVE: To explore the effect on DNA quantification and STR typing from cigarette butts collected at different time points. METHODS: Forty "Hongshuangxi" brand cigarette butts smoked by ten different individuals (4 cigarettes per individual) were collected. DNA was extracted from the outer layer and the sponge of the cigarette butts using chelex-100 extraction kit, as well as STR typing and DNA quantitation were simultaneously performed in 1, 4, 7 and 10 weeks, respectively. RESULTS: The DNA quantities extracted from the outer layer at the 1st, 4th, 7th and 10th week were 0.104-2.52, 0.110-2.41, 0.0960-2.32 and 0.085 0-2.28 ng/microL, while the detection rates for 16 loci by STR typing were 100%, 90%, 75% and 62.5%, respectively. The DNA quantities extracted from the sponge were 0.0180-2.40, 0.0171-2.25, 0.0165-2.15 and 0.0160-2.15 ng/microL, while the detection rates for 16 loci by STR typing were 97.5%, 82.5%, 50% and 12.5%, respectively. CONCLUSION: There is little difference in DNA quantity between the outer layer and the sponge of butts during 10 weeks, but there is an obvious effect on STR typing with prolonged extracting time. There is a much more effect on the sponge than on the outer layer, and the longer the standing time is, the lower the detection rate is.


Assuntos
Impressões Digitais de DNA/métodos , DNA/análise , Células Epiteliais/química , Repetições de Microssatélites/genética , Fumar , Genética Forense/métodos , Humanos , Mucosa Bucal/citologia , Fatores de Tempo
20.
Methods Appl Fluoresc ; 6(2): 024001, 2018 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-29350185

RESUMO

Cyanine has been widely utilized as a near infrared (NIR) fluorophore for detection of glutathione (GSH). However, the excitation of most of the reported cyanine-based probes was less than 800 nm, which inevitably induce biological background absorption and lower the sensitivity, limiting their use for detection of GSH in blood samples. To address this issue, here, a heptamethine cyanine probe (DNIR), with a NIR excitation wavelength at 804 nm and a NIR emission wavelength at 832 nm, is employed for the detection of GSH and its oxidized form (GSSG) in blood. The probe displays excellent selectivity for GSH over GSSG and other amino acids, and rapid response to GSH, in particular a good property for indirect detection of GSSG in the presence of enzyme glutathione reductase and the reducing agent nicotinamideadenine dinucleotide phosphate, without further separation prior to fluorescent measurement. To the best of our knowledge, this is the first attempt to explore NIR fluorescent approach for the simultaneous assay of GSH and GSSG in blood. As such, we expect that our fluorescence sensors with both NIR excitation and NIR emission make this strategy suitable for the application in complex physiological systems.


Assuntos
Carbocianinas/química , Glutationa/sangue , Espectroscopia de Luz Próxima ao Infravermelho , Corantes Fluorescentes/química , Glutationa/química , Humanos , NADP/química , Oxirredução
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