HPLC-DAD Analysis, SFE-CO2 Extraction, and Antibacterial Activity on Bioactive Compounds from Mosla chinensis Maxim.

Mosla chinensis Maxim is an annual herb with many potential purposes in agricultural, industrial, and pharmaceutical fields. At present, the extract of the whole plant from M. chinensis has been proven to demonstrate antifungal, antioxidant, and anti-inflammatory activities. Previous studies focused on the enzyme pretreatment in hydrodistillation from M. chinensis. However, organic solvent or supercritical fluid carbon dioxide extraction (SFE-CO2) methods, which are commonly utilized in industry, have seldom been studied and cannot provide multiple evaluations of yield. In this work, we analysed compounds from M. chinensis by HPLC-DAD, discussed n-hexane extraction, and conducted further investigations on SFE-CO2 through the design of response surface methodology (RSM). The sample obtained from pilot-scale SFE-CO2 was also tested against nine kinds of microorganisms. Single-factor results revealed that the extraction rates from M. chinensis by steam distillation, n-hexane extraction, and SFE-CO2 were 1%, 2.09%, and 3.26%, respectively. RSM results showed a significant improvement in extraction rate through optimising pressure and time, and the interaction of both factors was more important than that of temperature-pressure and temperature-time. A pilot-scale test with an extraction rate of 3.34% indicated that the predicted RSM condition was operable. In addition, samples from the pilot-scale SFE-CO2 showed antibacterial effects against three previously unreported bacteria (Gardnerella vaginalis, methicillin-resistant Staphylococcus aureus, and Propionibacterium acnes). These results fill the gap in previous research and provide more information for the application and development of M. chinensis in the future.

Wu-Mei-wan protects pancreatic ß cells by inhibiting NLRP3 Inflammasome activation in diabetic mice.

BACKGROUND: Wu-Mei-Wan (WMW) is a traditional Chinese herbal formulation that is clinically prescribed to treat diabetes mellitus in China. WMW has been shown to alleviate damage in pancreatic ß cells, but the underlying mechanism remains unclear. This study aims to explore how WMW plays a protective role in pancreatic islets. METHODS: Drug testing and mechanism analyses were performed on mice treated with three concentrations of WMW (4800, 9600, and 19,200 mg/kg/bw) for four consecutive weeks. Blood was collected from both db/db and wild-type mice to determine fasting blood glucose (FBG) and serum insulin levels. The expression of proteins related to apoptosis, cysteinyl aspartate-specific proteinase 12 (caspase-12) and B-cell leukemia 2 (Bcl-2), was measured by western blot. Interleukin-1ß (IL-1ß), interleukin-18 (IL-18), monocyte chemoattractant protein-1α (MCP-1α), and tumor necrosis factor-α (TNF-α) in the pancreas were tested with enzyme-linked immunosorbent assay (ELISA). Immunohistochemistry staining of F4/80 was performed to measure the pancreatic infiltration of macrophages. Western blot and immunofluorescence staining of the NLRP3 inflammasome were used to measure the expression of proteins related to apoptosis and inflammation. RESULTS: WMW dose-dependently reduced FBG and promoted serum insulin secretion in db/db mice compared to the wild-type controls. WMW protected pancreatic ß cells with a pattern of decreasing caspase-12 and increasing Bcl-2 expression. WMW also reversed the upregulated production of IL-1ß, IL-18, MCP-1α, and macrophage-specific surface glycoprotein F4/80 in diabetic mice. In addition, the protein expression levels of NLRP3 inflammasome components NLRP3, ASC, and caspase-1 (P20) were higher in db/db mice than in wild-type controls. CONCLUSIONS: WMW inhibits the activation of the NLRP3 inflammasome to protect pancreatic ß cells and prevent type 2 diabetes mellitus development.

[Effect of modified Zhengqi Powder in treating chronic gastritis and on patients' life quality and inflammatory factors].

Chronic gastritis is a kind of chronic gastric mucosal inflammation caused by many factors.Intestinal metaplasia refers to the transformation of gastric mucosal epithelial cells into small/large intestinal mucosal epithelium containing Panette or goblet cells.Chronic gastritis has the highest incidence among stomach diseases,while intestinal metaplasia is the serious manifestation of chronic gastritis.In this experiment,the therapeutic effect of modified Zhengqi Powder on mild intestinal metaplasia in chronic gastritis and on patients' quality of life and inflammatory reaction was investigated to analyze the efficacy and mechanism of traditional Chinese medicine prescription.From April 2016 to April 2017,120 patients of chronic gastritis with mild intestinal metaplasia were selected and divided into two groups according to the envelope method.The control group(60 cases) was treated with famoxetine.After one month of continuous treatment,the total effective rate of treatment in the observation group was 93.3%,which was much higher than 80.0% in the control group.Health questionnaire(SF-36),serum C-reactive protein(CRP),interleukin-8(IL-8) and tumor necrosis factor-α(TNF-α) levels were significantly higher than those in the control group(P<0.05).The results showed that modified Zhengqi Powder has a significant efficacy in treat chronic gastritis with mild intestinal metaplasia,and can obviously alleviate clinical symptoms and intestinal metaplasia,remove inflammatory factors and improve the quality of life of patients,and is worth promotion.

Anti-Inflammatory Triterpenoids from the Caulophyllum robustum Maximin LPS-Stimulated RAW264.7 Cells.

Caulophyllum robustum Maxim is widely distributed in China and used as a traditional herbal medicine to induce childbirth, ease the pain of labor, rectify delayed or irregular menstruation, alleviate heavy bleeding and pain during menstruation, and treat external injuries and irregular menses. According to our detailed chemical investigation, three new triterpene derivatives (1⁻3), together with seven known compounds, were isolated from the root and rhizome of C. robustum Maxim. Their structures were elucidated by 1D- and 2D-NMR spectroscopic analysis and physio-chemical methods. They were identified as (1) 23-hydroxy-3,19-dioxo-olean-12-en-28-oic-acid; (2) 23-hydroxy-3,11-dioxo-olean-12-en-28-oic acid; and (3) 16α,23-dihydroxy-3-oxo-olean-12-en-28-oic acid. Compounds (1⁻10) inhibited the LPS-activated NO production in RAW264.7 cells. Furthermore, the anti-inflammatory characteristics of these compounds were confirmed on the basis of decreases in iNOS and NF-κB protein expression in RAW264.7 cells.

Sexual health status of women who have regular sexual relations with men who have sex with men in mainland China.

BACKGROUND: Men who have sex with men (MSM) are a high-risk group for sexually transmitted diseases (STDs) and human immunodeficiency virus (HIV) infection. In China, the vast majority of MSM feel forced to marry or plan to marry women, according to traditional Chinese culture. Women who have regular sexual relations with MSM, called tongqi in mainland China, live with a high risk of STDs or HIV infection, but these risks are often ignored. Our investigation of this group of the women is a preliminary study that aims to understand the sexual health problems of tongqi and related factors. METHODS: This study relied on website mobilization and was funded by tongqi. Participants were limited to women who had sex with MSM to whom they were married (in-GWs), whom they had divorced (ex-GWs), or with whom they were friends (GGFs). The data were collected using questionnaire software. RESULTS: A total 144 valid surveys were returned from 100 in-GWs, 33 ex-GWs, and 11 GGFs. Average respondent age was 32.8 ± 6.4 years (range 22 to 58 years). Among in-GWs and ex-GWs, over 95% learned that their husbands were MSM after marriage. More than half of respondents had had sex before marriage, and one-third of those women had sex partners other than their husbands. In addition, 35.3% of tongqi had STDs symptoms. About 50% participants had had oral sex with sex partners of MSM and 10% had had passive anal sex, with low condom use during both oral (9.7%) and anal sex (23.1%). Most tongqi had misunderstandings about STDs and HIV and less than 30% had undergone HIV screening. Among participants tested, 5.6% were HIV positive. A total 93.5% of respondents believed that laws should be established to protect the sexual rights of women. CONCLUSIONS: Women who have regular sexual relations with MSM face adverse sexual health issues and are susceptible to STDs and HIV infection. Measures must be taken to protect the rights and interests of tongqi in mainland China.

[Chemical Constituents from Cremastra appendiculata].

OBJECTIVE: To study the chemical constituents of Cremastra appendiculata. METHODS: The compounds were isolated by repeated column chromatography with silica gel, Sephadex LH-20 and ODS-HPLC. The structures were elucidated by analysis of spectroscopic data(1H-NMR and 13C-NMR). RESULTS: Ten compounds were isolated from the EtOAc extract in the tuber of Cremastra appendiculata. Their structures were identified as shancigusin I (I), 4-O-ß-D-glucopyranosyl cinnamate (II), bulbocodin D (III), blestriarene A (IV), 7-hydroxy-2,4-dimethoxy phenanthrene (V), coelonin (VI), militarine (VII), gastrodine (VIII), 3-hydroxyphenylpropionic acid (IX) and cinnamic acid (X). CONCLUSION: Compounds I-III, IX and X are isolated from Cremastra appendiculata for the first time.

[Chemical Composition of n-Butanol Fraction from Polygonum amplexicaule var. sinense].

OBJECTIVE: To study chemical composition of n-butanol fraction from Polygonoum amplexicaule var. sinense. METHODS: TLC,normal-phase silica gel, reveres-phase silica gel, Sephadex-LH and semi-preparative HPLC were used to isolate chemical compositions of n-butanol fraction from Polygonoum amplexicaule var. sinense. RESULTS: Nine compounds were identified as: caffeic acid n-butly ester (1), p-methoxy benzoic acid propyl ester (2),p-E-coumarin quinic acid methyl ester (3),p-Z-coumarin quinic acid methyl ester (4), ethyl ferulate (5), cinchonain I a (6), cinchonain Ib (7), methyl chlorogenate(8), and 6-O-ß-D-caffeoylglucose (9). CONCLUSION: All compounds are isolated from this genus for the first time.

Exploring the Interplay between Stress Perception and Approach-Avoidance Achievement Goal Orientation among Rural Students in College: A Longitudinal Study in China.

The objective of this study was to examine the relationship between stress perception and achievement goals among rural college students in China. Using a two-wave random intercept cross-lagged model, this study analyzed data from a longitudinal survey conducted at 15 public universities in Beijing. First, there was a significant decline in stress levels among Chinese rural students from their junior to senior years. Second, the achievement goal orientations of these students remained relatively stable during the period. Third, while no significant association was found between approach goals and stress perception, a clear positive reciprocal relationship emerged between avoidance goals and stress perception. These results suggest that educators should focus on alleviating the stress experienced by rural college students through academic, employment, and interpersonal support to break the vicious cycle of stress perception and avoidance goal orientation. Future research should further explore the influence of diverse group characteristics and emotional states on these outcomes.

Climate change, ambient air pollution, and students' mental health.

The impact of global climate change and air pollution on mental health has become a crucial public health issue. Increased public awareness of health, advancements in medical diagnosis and treatment, the way media outlets report environmental changes and the variation in social resources affect psychological responses and adaptation methods to climate change and air pollution. In the context of climate change, extreme weather events seriously disrupt people's living environments, and unstable educational environments lead to an increase in mental health issues for students. Air pollution affects students' mental health by increasing the incidence of diseases while decreasing contact with nature, leading to problems such as anxiety, depression, and decreased cognitive function. We call for joint efforts to reduce pollutant emissions at the source, improve energy structures, strengthen environmental monitoring and gover-nance, increase attention to the mental health issues of students, and help student groups build resilience; by establishing public policies, enhancing social support and adjusting lifestyles and habits, we can help students cope with the constantly changing environment and maintain a good level of mental health. Through these comprehensive measures, we can more effectively address the challenges of global climate change and air pollution and promote the achievement of the United Nations Sustainable Development Goals.

Using ChatGPT to promote college students' participation in physical activities and its effect on mental health.

As one of the most famous large language models, ChatGPT has great potential for application in physical education. It can provide personalized exercise plans, a variety of exercise options, and interactive support. The integration of ChatGPT into the teaching process can promote college students' participation in physical activities and improve their mental health while expanding the traditional teaching environment and promoting the reform of traditional teaching methods. However, the application of ChatGPT faces challenges and obstacles in physical education. To make full use of ChatGPT in physical education, it can be combined with wearable devices and sports equipment to enhance the efficiency of interactions with users. Relevant policies are urgently needed to avoid the improper use of users' data.

Adolescent suicide risk factors and the integration of social-emotional skills in school-based prevention programs.

Adolescents are considered one of the most vulnerable groups affected by suicide. Rapid changes in adolescents' physical and mental states, as well as in their lives, significantly and undeniably increase the risk of suicide. Psychological, social, family, individual, and environmental factors are important risk factors for suicidal behavior among teenagers and may contribute to suicide risk through various direct, indirect, or combined pathways. Social-emotional learning is considered a powerful intervention measure for addressing the crisis of adolescent suicide. When deliberately cultivated, fostered, and enhanced, self-awareness, self-management, social awareness, interpersonal skills, and responsible decision-making, as the five core competencies of social-emotional learning, can be used to effectively target various risk factors for adolescent suicide and provide necessary mental and interpersonal support. Among numerous suicide intervention methods, school-based interventions based on social-emotional competence have shown great potential in preventing and addressing suicide risk factors in adolescents. The characteristics of school-based interventions based on social-emotional competence, including their appropriateness, necessity, cost-effectiveness, comprehensiveness, and effectiveness, make these interventions an important means of addressing the crisis of adolescent suicide. To further determine the potential of school-based interventions based on social-emotional competence and better address the issue of adolescent suicide, additional financial support should be provided, the combination of social-emotional learning and other suicide prevention programs within schools should be fully leveraged, and cooperation between schools and families, society, and other environments should be maximized. These efforts should be considered future research directions.

Potential use of large language models for mitigating students' problematic social media use: ChatGPT as an example.

The problematic use of social media has numerous negative impacts on individuals' daily lives, interpersonal relationships, physical and mental health, and more. Currently, there are few methods and tools to alleviate problematic social media, and their potential is yet to be fully realized. Emerging large language models (LLMs) are becoming increasingly popular for providing information and assistance to people and are being applied in many aspects of life. In mitigating problematic social media use, LLMs such as ChatGPT can play a positive role by serving as conversational partners and outlets for users, providing personalized information and resources, monitoring and intervening in problematic social media use, and more. In this process, we should recognize both the enormous potential and endless possibilities of LLMs such as ChatGPT, leveraging their advantages to better address problematic social media use, while also acknowledging the limitations and potential pitfalls of ChatGPT technology, such as errors, limitations in issue resolution, privacy and security concerns, and potential overreliance. When we leverage the advantages of LLMs to address issues in social media usage, we must adopt a cautious and ethical approach, being vigilant of the potential adverse effects that LLMs may have in addressing problematic social media use to better harness technology to serve individuals and society.

Music recommendation algorithms based on knowledge graph and multi-task feature learning.

During music recommendation scenarios, sparsity and cold start problems are inevitable. Auxiliary information has been utilized in music recommendation algorithms to provide users with more accurate music recommendation results. This study proposes an end-to-end framework, MMSS_MKR, that uses a knowledge graph as a source of auxiliary information to serve the information obtained from it to the recommendation module. The framework exploits Cross & Compression Units to bridge the knowledge graph embedding task with recommendation task modules. We can obtain more realistic triple information and exclude false triple information as much as possible, because our model obtains triple information through the music knowledge graph, and the information obtained through the recommendation module is used to determine the truth of the triple information; thus, the knowledge graph embedding task is used to perform the recommendation task. In the recommendation module, multiple predictions are adopted to predict the recommendation accuracy. In the knowledge graph embedding module, multiple calculations are used to calculate the score. Finally, the loss function of the model is improved to help us to obtain more useful information for music recommendations. The MMSS_MKR model achieved significant improvements in music recommendations compared with many existing recommendation models.

Unlocking the power of physical activity in easing psychological distress.

The severity of the current global mental health situation and the importance of maintaining psychological well-being call for more powerful, convenient, and efficient solutions for addressing psychological issues and relieving mental stress. Physical activity not only effectively improves physical fitness and reduces negative emotions such as anxiety and depression but also increases the improvement of psychological health and sense of well-being. At the same time, physical activity interventions for mental health have unique advantages, including reducing the side effects of psychological interventions and increasing necessity, convenience, and cost-effectiveness, as well as flexible adaptability across multiple methods, groups, and age ranges, providing stronger support for relieving psychological stress and addressing psychological issues. Although physical activity is an important intervention measure in relieving psychological stress, its value and role in mental health care seem to have not yet received sufficient attention, and its potential remains to be further revealed. Given the significant advantages and effectiveness of physical activity in mental health intervention practices, it is necessary to stimulate its potential in relieving psychological stress through various means in future studies to better safeguard the public's physical and mental health. Developing guidelines for physical activity for improved mental health, enhancing organic integration with other inter-vention measures, and providing necessary respect, encouragement, and support are important directions to consider.

Depression and approach-avoidance achievement goals of Chinese undergraduate students: A four-wave longitudinal study.

OBJECTIVE: The study explored the prospective relations between depression and approach-avoidance achievement goals of undergraduate students in China. METHODS: 2473 full-time undergraduates reported their depression and achievement goals annually from the freshman to the senior year. Data were analysed using descriptive statistics, correlation analysis and cross-lagged models. RESULTS: Students' achievement goals decreased gradually during the first 3 years but rose in the fourth year, and the avoidance goals appeared to be less prevalent than the approach goals over time. Depression was negatively associated with approach goals, whereas positively correlated with avoidance goals. Depression in the freshman and sophomore years resulted in more avoidance goals 1 year later, and the depressive problems in the junior year predicted the decline of approach goals in the senior year. CONCLUSIONS: The present study highlighted the deleterious effects of depression on the achievement goals of college students.

The Relationship between Stress and Academic Self-Efficacy among Students at Elite Colleges: A Longitudinal Analysis.

Stress and academic self-efficacy are crucial factors in the psychological health of college students. Previous research has shown that stress is associated with academic self-efficacy, but their longitudinal relationships among students at elite colleges remain unclear. This study aimed to explore the longitudinal relationships between stress and academic self-efficacy among students from five elite colleges in China. Descriptive statistics indicated that students experienced a slight reduction in stress accompanied by a marginal increase in academic self-efficacy from the junior year to the senior year. Correlation analysis revealed that stress was negatively correlated with academic self-efficacy. According to cross-lagged models, heightened stress significantly predicted lower levels of academic self-efficacy. However, greater academic self-efficacy did not significantly predict lower stress. In conclusion, stress exhibited a unidirectional negative prediction on academic self-efficacy over time among students at elite colleges. The results of this study suggested that elite colleges should pay more attention to the mental health of students and provide appropriate guidance, such as establishing a positive mental health atmosphere in the educational environment and employing advanced technological means.

Oleanane triterpenoids with PTP1B and α-glucosidase inhibitory activities from Caulophyllum robustum.

Eleven oleanane triterpenoids (1-11) including two new ones (1 and 2) were isolated from the roots and stems of Caulophyllum robustum. Their structures were established by extensive spectroscopic analysis, comparison with literature, and NMR calculations. Compounds 1 and 2 represent the first examples of 23-hydroxy-28-nor-oleanane and 21-hydroxy-olean-3-one triterpenoids, respectively. All isolates were evaluated for their PTP1B and α-glucosidase inhibitory activities in vitro. Among them, the triterpene aglycones 1-5 showed almost equivalent PTP1B inhibitory activities to oleanolic acid and ursolic acid, while 1, 2, and the triterpene saponins 6-11 showed significant α-glucosidase inhibitory activities. Furthermore, compounds 1 and 3 were proved to regulate the expression of proteins implicated the PTP1B/IRS-1/pIRS-1 signalling pathway to improve insulin resistance.

Current situation and influence factors of scientific integrity in China: A multicenter survey.

Objective: Cases of scientific misconduct have occurred frequently, especially in the field of medical research. We collected electronic questionnaires from 1257 medical staff in 43 cities and obtained a cross-sectional data set of their understanding of scientific integrity in research. This study aims to propose recommendations for establishing a mature oversight system for research integrity. Methods: The study employed multiple regression analysis to explore the effect of different factors on the perception of four types of research integrity. Results: Female participants had a higher understanding of project application integrity than men (P < â€‹ 0.001). Participants in clinical departments had a lower understanding of project application integrity than those in nursing departments (clinical vs. nursing, P â€‹= 0.046). Participants with a junior college degree or below had a lower understanding than those who had a postgraduate degree and doctoral degree (junior college or below vs. postgraduate degree, P â€‹< â€‹0.001; junior college or below vs. doctoral degree, P â€‹< â€‹0.001). Conclusions: We found that female, medical technology department, advanced education background, and advanced professional titles were significantly associated with a higher understanding of scientific integrity in research in China.

[Treating influenza patients of wind-heat affecting Fei syndrome by jinhua qinggan granule: a double-blinded randomized control trial].

OBJECTIVE: To assess the effect and safety of Jinhua Qinggan Granule (JHG) in treating influenza patients of wind-heat affecting Fei syndrome (WHAFS). METHODS: Totally 136 influenza patients of WHAFS were randomized by stratification into 3 groups, the high dose JHG group (44 cases, 10 g each time), the low dose JHG group (45 cases, 5 g JHG + 5 g placebo each time), and the placebo control group (47 cases, 10 g placebo each time). All medication was administered three times daily for 5 days. The fever disappearance time, the fever disappearance rate, efficacy of TCM syndrome, the disappearance rate of main symptoms and physical signs of flu, the negative rate of virus nucleic acid in the pharyngeal secretion, and safety indicators were assessed. RESULTS: The median fever disappearance time was 32.8 h (95% CI: 22.5-41.0 h) in the high dose JHG group, 26.0 h (95% CI: 14.5-36.5 h) in the low dose JHG group, 39.5 h (95% CI: 29.0-46.0 h) in the placebo control group. There was statistical difference in the median fever disappearance time between the low dose JHG group and the placebo control group (P = 0.011). Three days after treatment, the markedly effective rate of TCM symptoms in the low dose JHG group was 66.7%, higher than that of the placebo control group (38.3%), and its effective rate was superior to that of the high dose JHG group (P = 0.043). Five days after treatment, the recovery rate of the low dose JHG group (42.2%) was higher than that of the high dose JHG group (25.0%, P = 0.026) and that of the placebo control group (14.9%, P = 0.002). The markedly effective rate of the low dose JHG group (86.7%) was higher than that of the placebo control group (55.3%, P = 0.001). Similar effects were obtained in the low dose JHG group and the high dose JHG group, but slightly poor in partial indicators of the high dose JHG group. There was no statistical difference in adverse reaction among these three groups (P > 0.05). CONCLUSIONS: JHG was effective and safe in treating influenza patients of WHAFS. Routinely low dose was the optimal dosage of JHG.

Understanding the Role of Parent‒Child Relationships in Conscientiousness and Neuroticism Development among Chinese Middle School Students: A Cross-Lagged Model.

The parent‒child relationship is a crucial factor in promoting adolescent mental health. However, the current evidence on the relationship between parent‒child relationships and adolescent conscientiousness and neuroticism, as well as the directionality of these relationships, remains limited. In particular, there is a lack of analysis focusing on Chinese middle school students. Based on a sample of 8437 students from the China Education Panel Survey (CEPS) database, this study empirically examined the bidirectional relationships between parent‒child relationships, conscientiousness and neuroticism among Chinese middle school students, with specific emphasis on the significant role of parent‒child relationships in the development of conscientiousness and neuroticism. Descriptive statistical results indicated that during the seventh and eighth grades of Chinese middle school students, the closeness of their parent‒child relationships with both parents decreased, while the level of conscientiousness showed a slight decrease, and neuroticism showed an increasing trend. Correlational results demonstrated a significant positive correlation between parent‒child relationships and conscientiousness and a significant negative correlation between parent‒child relationships and neuroticism. Further analysis using cross-lagged models revealed that parent‒child relationships significantly positively predicted subsequent conscientiousness development, and conscientiousness significantly positively predicted subsequent parent‒child relationships. Parent‒child relationships significantly negatively predicted subsequent neuroticism development, and neuroticism levels also significantly negatively predicted subsequent parent‒child relationships. Based on these findings, we believe that there is a need to strengthen parent‒child relationships and to recognize the important role that both mothers and fathers play in the healthy development of their children. Both parents should actively contribute to their children's upbringing and take responsibility for their family education.