RESUMO
Transparency in the lens is accomplished by the dense packing and short-range order interactions of the crystallin proteins in fiber cells lacking organelles. These features are accompanied by a lack of protein turnover, leaving lens proteins susceptible to a number of damaging modifications and aggregation. The loss of lens transparency is attributed in part to such aggregation during aging. Among the damaging post-translational modifications that accumulate in long-lived proteins, isomerization at aspartate residues has been shown to be extensive throughout the crystallins. In this study of the human lens, we localize the accumulation of l-isoaspartate within water-soluble protein extracts primarily to crystallin peptides in high-molecular weight aggregates and show with MS that these peptides are from a variety of crystallins. To investigate the consequences of aspartate isomerization, we investigated two αA crystallin peptides 52LFRTVLDSGISEVR65 and 89VQDDFVEIH98, identified within this study, with the l-isoaspartate modification introduced at Asp58 and Asp91, respectively. Importantly, whereas both peptides modestly increase protein precipitation, the native 52LFRTVLDSGISEVR65 peptide shows higher aggregation propensity. In contrast, the introduction of l-isoaspartate within a previously identified anti-chaperone peptide from water-insoluble aggregates, αA crystallin 66SDRDKFVIFL(isoAsp)VKHF80, results in enhanced amyloid formation in vitro The modification of this peptide also increases aggregation of the lens chaperone αB crystallin. These findings may represent multiple pathways within the lens wherein the isomerization of aspartate residues in crystallin peptides differentially results in peptides associating with water-soluble or water-insoluble aggregates. Here the eye lens serves as a model for the cleavage and modification of long-lived proteins within other aging tissues.
Assuntos
Cristalinas/química , Ácido Isoaspártico/química , Cristalino/metabolismo , Agregados Proteicos , Sequência de Aminoácidos , Cromatografia Líquida de Alta Pressão , Cristalinas/metabolismo , Humanos , Isomerismo , Espectrometria de Massas , Peptídeos/análise , Peptídeos/química , Peptídeos/isolamento & purificação , Proteína D-Aspartato-L-Isoaspartato Metiltransferase/genética , Proteína D-Aspartato-L-Isoaspartato Metiltransferase/metabolismo , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/química , Proteínas Recombinantes/isolamento & purificação , Cadeia A de alfa-Cristalina/química , Cadeia A de alfa-Cristalina/genética , Cadeia A de alfa-Cristalina/metabolismo , Cadeia B de alfa-Cristalina/química , Cadeia B de alfa-Cristalina/genética , Cadeia B de alfa-Cristalina/metabolismoRESUMO
A novel haloalkaliphilic archaeon, strain B23(T) was isolated from the former lake Texcoco in Mexico. The strain was Gram-stain-negative, the cells coccoid to ovoid rods, red pigmented and aerobic. Strain B23(T) grew in 1.7-4.3 M NaCl, at pH 6.5-9.5 and at 25-45 °C with optimal growth at 2.6-3.4 M NaCl, pH 7.5-8.5 and 37 °C. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain B23(T) was most closely related to Natronobacterium gregoryi SP2(T) with 97.3â% sequence similarity. The polar lipids of strain B23(T) were phosphatidylglycerol and several unidentified phospholipids. The G+C content of the DNA of the strain was 62.5 mol%. Levels of DNA-DNA relatedness between strain B23(T) and Natronobacterium gregoryi DSM 3393(T) was 32.3â%. The name Natronobacterium texcoconense sp. nov. is proposed. The type strain is B23(T) (â=âCECT 8068(T)â=âJCM 17655(T)).
Assuntos
Natronobacterium/classificação , Filogenia , Microbiologia do Solo , Composição de Bases , DNA Arqueal/genética , Concentração de Íons de Hidrogênio , Lagos , México , Dados de Sequência Molecular , Natronobacterium/genética , Natronobacterium/isolamento & purificação , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , SalinidadeRESUMO
We present the case of a female patient with complications from superficial implantation of polydioxanone spiculated threads in the facial middle third. The manifestations were edema, ecchymosis, superficial palpation of the thread and skin plication. For the treatment we propose a novel protocol of clinical degradation of polydioxanone threads, using hyaluronic acid injecting it into the implantation area of the polydioxanone threads. Non-cross linked hyaluronic acid was infiltrated along the path of the thread. The follow-up at seven, 21 and 45 days showed decreased edema, folds and skin irregularities due to wire traction and attenuation of secondary rhytides. A sample of the thread in hyaluronic acid was observed in a laminar flow campaign evidencing degradation at 72 hours. Administering hyaluronic acid was effective in inducing clinical biodegradation of the thread suggesting that non-crosslinked hyaluronic acid is a powerful catalyst for the hydrolytic degradation of polydioxanone.
Se expone el caso de una paciente remitida por complicaciones posteriores a la implantación superficial en el tercio medio facial de hilos de polidioxanona espiculados. Presentaba edema, equimosis, palpación superficial del hilo y plicación dérmica. Para el tratamiento planteamos un novedoso protocolo de degradación clínica de hilos de polidioxanona, empleando ácido hialurónico inyectándolo en el área de su implantación. Se infiltró ácido hialurónico no reticulado en el trayecto a lo largo del recorrido del hilo. El seguimiento a los siete, 21 y 45 días evidenció disminución del edema, de los pliegues e irregularidades dérmicas por la tracción del hilo y atenuación de rítides secundarias. Una muestra del hilo en ácido hialurónico fue observado en campana de flujo laminar evidenciándose degradación a las 72 horas. Administrar ácido hialurónico fue efectivo para inducir la biodegradación clínica del hilo. La hipótesis derivada sugiere que el ácido hialurónico no reticulado es un poderoso catalizador de la degradación hidrolítica de la polidioxanona.
Assuntos
Ácido Hialurônico/química , Polidioxanona/efeitos adversos , Ritidoplastia/efeitos adversos , Adulto , Feminino , Seguimentos , Humanos , Microscopia/métodos , Polidioxanona/química , Ritidoplastia/métodos , Envelhecimento da PeleRESUMO
The amount of nanoparticles (NP), such as TiO2, has increased substantially in the environment. It is still largely unknown, however, how NP might interact with earthworms and organic material and how this might affect the bacterial community structure and their functionality. Therefore, an arable soil was amended with TiO2 NP at 0, 150 or 300 mg kg-1 and subjected to different treatments. Treatments were soil amended with ten earthworms (Eisenia fetida (Savigny, 1826)) with fully developed clitellum and an average fresh mass of 0.5 to 500 g dry soil, 1.75 g tyndallized Quaker® oat seeds Avena sativa (L.) kg-1, or earthworms plus oat seeds, or left unamended. The bacterial community structure was monitored throughout the incubation period. The bacterial community in the unamended soil changed over time and application of oats, earthworm and a combination of both even further, with the largest change found in the latter. Application of NP to the unamended soil and the earthworm-amended soil altered the bacterial community, but combining it by adding oats negated that effect. It was found that the application of organic material, that is, oats, reduced the effect of the NP applied to soil. However, as the organic material applied was mineralized by the soil microorganisms, the effect of NP increased again over time.
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Introducción: el granuloma ulcerativo traumático con eosinofilia estromal es una afección benigna, crónica y autolimitante, que por su evolución clínica puede estar sujeta a confusión diagnóstica. Por ello, el caso que aquí se comparte expone particularidades de esta afección y su respuesta al tratamiento para permitir un mejor conocimiento de esta lesión. Se describen las características clínicas e histopatológicas y su evolución ante la terapéutica empleada. Se presenta una paciente femenina de seis años, con antecedentes de salud y de dientes neonatales, que hace tres meses manifiesta dos úlceras en mucosa sublingual que no cicatrizan ni mejoran al tratamiento anterior. Se indicaron estudios hematológicos, se realizó biopsia incisional de la lesión con su estudio histopatológico e inmunohistoquímico. Se obtuvo eosinofilia estromal y ausencia de linfocitos anómalos CD30. El tratamiento incluyó aplicación de corticosteroides tópicos e intralesionales, experimentando remisión de la lesión. Se mantiene la paciente bajo seguimiento clínico, sin recidiva de lesión. Conclusiones: el granuloma ulcerativo traumático con eosinofilia estromal es una lesión autolimitante que puede ser confundida con otras lesiones ulcerativas de la cavidad bucal, por lo que su estudio histopatológico resulta imprescindible para su diagnóstico. Asimismo, su análisis inmunohistoquímico es indispensable para precisar su naturaleza y probable evolución. El adecuado y exhaustivo proceso diagnóstico constituye una herramienta vital para lograr su identificación.
Background: Traumatic ulcerative granuloma with stromal eosinophilia is a benign, chronicle and self-healing lesion, which can be misdiagnosed for its clinical evolution, for this reason, this case report is carried out, showing particularities of this disease and its response in front to the treatment, giving a better identifcation of the lesion, were described the clinical and histopathological fndings of a case. A feminine patient, six years old, with neonatal health and dental history. She has presented during three months two ulcerative lesions in sublingual mucosa, which do not improve with the previous treatment. Hematological studies and biopsy were carried out, the incisional biopsy was analyzed with immunohistochemical test, the results were stromal eosinophilia and absence of anomalous lymphocytes CD30. She was treated with topical and intralesional corticosteroids, experiencing remission of the lesion. The patient had a long clinical follow up without recidive. Traumatic ulcerative granuloma with stromal eosinophilia is a self-healed lesion that needs a histopathological and inmunohistochemical analysis for an adequate diagnosis. The correct diagnostic sequence is a vital tool to achieve its identification.
Assuntos
Feminino , Criança , Úlcera , Eosinofilia , Granuloma , Biópsia , Imuno-HistoquímicaRESUMO
BACKGROUND AND OBJECTIVES: Screening colonoscopy with polipectomy reduces colonorectal cancer incidence and mortality. An adequate bowel cleansing is one of the keys to achieving best results with this technique. Oral sodium phosphate solution (OSP) had a widespread use in the 90s decade. Its efficacy was similar to polyethylene glycol (PEG) solution, but with less cost and convenient administration. Series of patients with acute renal failure due to OSP use have been reported. However, large cohorts of patients found no difference in the incidence of renal damage between these two solutions. METHODS: From 2006 to 2009 we identified twelve cases of phosphate nephropathy after colonoscopy prepared with OSP. All patients were followed up to six months. All patients had received just a single dose. RESULTS: We analyzed 12 cases with phosphate nephropathy; three patients debuted with AKI and nine patients had chronic renal injury. Four cases were confirmed with renal biopsy. One patient with AKI needed hemodialysis at diagnosis without subsequent recovery. Two patients (both with chronic damage) fully recovered their previous renal function. The remaining patients (nine) had an average loss of estimated glomerular filtration rate of 24ml/min/1.73m(2). CONCLUSIONS: The use of OSP can lead to both acute and chronic renal damage. However, chronic injury was the most common pattern. Both forms of presentation imply a significant and irreversible loss of renal function. Further studies analyzing renal damage secondary to bowel cleaning should consider these two different patterns of injury.
Assuntos
Injúria Renal Aguda/induzido quimicamente , Catárticos/efeitos adversos , Fosfatos/efeitos adversos , Insuficiência Renal Crônica/induzido quimicamente , Idoso , Idoso de 80 Anos ou mais , Anti-Hipertensivos/farmacocinética , Fosfatos de Cálcio/análise , Catárticos/farmacocinética , Colonoscopia , Creatinina/sangue , Interações Medicamentosas , Feminino , Seguimentos , Taxa de Filtração Glomerular , Humanos , Masculino , Pessoa de Meia-Idade , Fosfatos/farmacocinética , Insuficiência Renal Crônica/sangue , Insuficiência Renal Crônica/patologiaRESUMO
BACKGROUND AND OBJECTIVES: Several studies have suggested that activation of the complement system is a contributing pathogenic mechanism in IgA nephropathy (IgAN). C4d staining is an inexpensive and easy-to-perform method for the analysis of renal biopsies. This study aimed to assess the clinical and prognostic implications of C4d staining in IgAN. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: This retrospective cohort study included 283 patients with IgAN in 11 hospitals in Spain who underwent a renal biopsy between 1979 and 2010. The primary predictor was mesangial C4d staining. Secondary predictors included demographic, clinical, and laboratory characteristics, and Oxford pathologic classification criteria. The primary end point was the cumulative percentage of patients who developed ESRD, defined as onset of chronic dialysis or renal transplantation. C4d was analyzed by immunohistochemical staining using a polyclonal antibody. Kaplan-Meier and Cox proportional hazards analyses were performed to evaluate the effect of C4d staining on renal survival. RESULTS: There were 109 patients (38.5%) and 174 patients (61.5%) who were classified as C4d positive and C4d negative, respectively. Renal survival at 20 years was 28% in C4d-positive patients versus 85% in C4d-negative patients (P<0.001). Independent risk factors associated with ESRD were as follows: proteinuria (hazard ratio [HR] per every 1 g/d increase. 1.16; 95% confidence interval [95% CI], 1.03 to 1.31; P=0.01), eGFR (HR per every 1 ml/min per 1.73 m(2) increase, 0.96; 95% CI, 0.94 to 0.97; P<0.001), T2 Oxford classification (tubular atrophy/interstitial fibrosis, >50%; HR, 4.42; 95% CI, 1.40 to 13.88; P=0.01), and C4d-positive staining (HR, 2.45; 95% CI, 1.30 to 4.64; P=0.01). CONCLUSIONS: C4d-positive staining is an independent risk factor for the development of ESRD in IgAN. This finding is consistent with the possibility that complement activation is involved in the pathogenesis of this disease.
Assuntos
Complemento C4b/análise , Doença Hepática Terminal/fisiopatologia , Glomerulonefrite por IGA/patologia , Glomerulonefrite por IGA/fisiopatologia , Rim/patologia , Células Mesangiais/química , Fragmentos de Peptídeos/análise , Adulto , Biópsia , Progressão da Doença , Doença Hepática Terminal/etiologia , Doença Hepática Terminal/metabolismo , Doença Hepática Terminal/patologia , Feminino , Taxa de Filtração Glomerular , Glomerulonefrite por IGA/complicações , Glomerulonefrite por IGA/metabolismo , Humanos , Hipertelorismo/complicações , Imuno-Histoquímica , Estimativa de Kaplan-Meier , Rim/química , Masculino , Pessoa de Meia-Idade , Prognóstico , Proteinúria/etiologia , Estudos Retrospectivos , Fatores de Risco , Adulto JovemRESUMO
Introducción: Las enfermedades cardiovasculares constituyen la primera causa de mortalidad a nivel mundial. Las causas implicadas en las mismas están bien definidas y son los denominados factores de riesgo cardiovascular. Es de utilidad conocer la frecuencia de estos factores en la población adulta joven, de modo a tomar medidas preventivas. Objetivo: determinar la prevalencia de hipertensión arterial, diabetes mellitus, sobrepeso, obesidad y tabaquismo en población joven del área urbana de la ciudad de Coronel Oviedo, Paraguay. Material y método: se realizó un estudio, observacional, descriptivo de corte transversal, entre los meses de junio a agosto del 2012. Se incluyó a 139 sujetos entre 20 y 40 años, del área urbana de la ciudad de Coronel Oviedo, seleccionados mediante muestreo aleatorio simple, utilizando las recomendaciones del método progresivo paso a paso (steps) de vigilancia de factores de riesgo, de la Organización Mundial de la Salud y la Organización Panamericana de la Salud. Resultados: la edad media fue 28,8 años, la distribución según sexo fue de 63% femenino y 37% masculino. El estudio demostró que 21% padece de hipertensión arterial, 8% diabetes mellitus, 36% sobrepeso, 14% obesidad y 18% tabaquismo. El 61% de los encuestados presentaba factores de riesgo cardiovascular, distribuidos de la siguiente manera: 1 factor de riesgo 37%, 2 factores de riesgo 19% y 3 o más factores de riesgo 6%. Conclusiones: los factores de riesgo cardiovascular más frecuentes fueron el sobrepeso, hipertensión arterial y tabaquismo.
Introduction: Cardiovascular diseases are the first cause of mortality worldwide. The implicated causes of them are well defined and are called cardiovascular risk factors. It is useful to know the frequency of these factors in the young adult population in order to take preventive measures. Objective: To determine the prevalence of arterial hypertension, diabetes mellitus, overweight, obesity and smoking in the young population of the urban area of Coronel Oviedo, Paraguay. Material and methods: A cross-sectional, descriptive observational study was carried out from June to August, 2012. One hundred thirty nine subjects, who were between 20 and 40 years of age from the urban area of Coronel Oviedo, were included. They were selected by simple random sampling using the recommendations of the stepwise approach to Surveillance (STEPS) for risk factors of the World Health Organization and the Pan American Health Organization. Results: Mean age was 28.8 years, distribution by sex was 63% women and 37% men. The study showed that 21% suffered arterial hypertension, 8% diabetes mellitus, 36% overweight, 14% obesity and 18% smoking. Sixty one percent presented cardiovascular risk factors distributed as follows: one risk factor 37%, two risk factors 19% and three or more risk factors 6%. Conclusions: The most frequent cardiovascular risk factors were overweight, arterial hypertension and smoking.
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Introducción: las lesiones proliferativas son entidades que se presentan en la cavidad bucal. Algunas son de origen traumático y otras son neoplasias. Con frecuencia no son bien diagnosticadas en la clínica, por la similitud que pueden presentarse entre ellas. Objetivo: determinar las características de las lesiones proliferativas diagnosticadas y la coincidencia entre el diagnóstico clínico y el histopatológico. Método: se realizó un estudio retrospectivo de las biopsias procesadas en cinco años en el Departamento de Patología de la Facultad de Estomatología de La Habana atendiendo a edad y sexo del paciente, tipo de lesión, localización anatómica y coincidencia entre el diagnóstico clínico y el histopatológico. Resultados: se encontró que de las lesiones proliferativas, las pseudotumorales aportaron el 18,1 por ciento y las neoplasias benignas el 5,8 por ciento. En el grupo de edad de 10 a 19 años las lesiones pseudotumorales representaron el 7,78 por ciento y las neoplasias benignas el 1,78 por ciento. En el grupo de 60 años o más, las lesiones pseudotumorales aportaron el 22,67 por ciento del total de lesiones proliferativas. El sexo femenino resultó afectado por lesiones pseudotumorales en 61,3 por ciento y las neoplasias benignas afectaron al sexo masculino en un 55 por ciento. La hiperplasia fibroepitelial representó el 54,22 por ciento de las pseudotumorales, mientras que el nevus aportó el 8,67 por ciento entre las neoplasias benignas. Los diagnósticos clínicos de las lesiones pseudotumorales coincidieron en un 50,4 por ciento con los histológicos, para las neoplasias benignas la coincidencia fue del 53,2 por ciento. Conclusiones: las lesiones pseudotumorales predominaron sobre las neoplasias benignas, con un incremento marcado de ambos tipos de lesiones a partir de los 40 años. En el sexo femenino predominaron las lesiones pseudotumorales, y en el masculino predominaron las neoplasias benignas. Se encontró una baja coincidencia entre el diagnóstico clínico e histopatológico para ambos tipos de lesiones(AU)
Introduction: proliferative lesions are entities that are present in the buccal cavity. Some of them are of traumatic origin and others are neoplasias. Frequently, they are not well diagnosed in the clinical practice because of the similarity that can exist among them. Objective: to determine the characteristics of the proliferative lesions diagnosed and the coincidences between clinical and histopathological diagnosis Method: a retrospective study of the biopsies processed during five years in the Department of Oral Pathology of the Faculty of Stomatology of Havana was conducted dealing with age, sex, type of lesion, anatomic location and similarities between the clinical and histopathological diagnosis. Results: it was found that, of the proliferative lesions, the pseudotumoral ones reported the 18.1 percent and the benign neoplasias, the 5.8 percent. In the age group 10-19 years, pseudotumoral lesions represented the 7.78 percent and the benign neoplasias the 1,78 percent. In the group of 60 years or older, pseudotumoral lesions reported the 22.67 percent of the total of proliferative lesions. The female sex resulted to be affected by pseudotumoral lesions in a 61,3 percent and the benign neoplasias effected the male sex in a 55 percent. Fibroepithelial hyperplasia represented the 54.22 percent of the pseudotumoral ones, whereas the nevus reported the 8.67 percent among the benign neoplasias. The clinical diagnosis of the pseudotumoral lesions matched up a 50.4 percent with the histological; for benign neoplasias, the coincidence was 53.2 percent. Conclusions: pseudotumoural lesions predominated on benign neoplasias, with a marked increase in both types of lesions after 40 years. Pseudotumoural lesions predominated in the female sex, whereas the benign neoplasias predominated in the male sex. There was a low coincidence between the clinical and histopathological diagnosis for both types of lesions(AU)
Assuntos
Humanos , Masculino , Feminino , Adolescente , Pessoa de Meia-Idade , Biópsia/efeitos adversos , Neoplasias Faciais/patologia , Neoplasias Maxilomandibulares/diagnóstico , Neoplasias Maxilomandibulares/patologia , Boca/lesões , Estudos RetrospectivosRESUMO
Objetivo: Evaluación de las características de las publicaciones sobre drogas realizadas en Costa Rica entre 1966 y 2010. Materiales y Métodos: Análisis bibliométrico de los trabajos compilados en la serie “Investigaciones sobre Alcoholismo y Farmacodependencia” que publica el Instituto sobre Alcoholismo y Farmacodependencia de Costa Rica de publicación quinquenal. Dicha serie se construyó a partir de una hoja de registro con diversas características de interés, tras realizarse una consulta de los catálogos y bases de datos de las bibliotecas y centros de documentación de diferentes instituciones. Resultados: Se estudiaron 491publicaciones. Una tercera parte se publicó en revistas científicas en tanto las publicaciones monográficas y los libros ocuparon, respectivamente, un 33 y un 6 % del total. Cerca de una tercera parte de los trabajos son investigaciones epidemiológicas y la droga mayormente investigada fue el alcohol, en ámbitos poblacionales que comprendieron las evaluaciones en la población general, los sujetos en calidad de pacientes y el sector infancia y juventud. Discusión: Se destaca el papel que ha correspondido a las ciencias sociales en el terreno del conocimiento sobre drogas, la importancia y necesidad de una mayor presencia femenina en la producción de dichos conocimientos y la exploración del fenómeno mediante alternativas que supongan un mayor énfasis tanto en el paradigma cualitativo como en el experimental y cuasi experimental para el abordaje de las complejidades que el tema exige.
Objective: This study analyses the characteristics of the publications on drugs carried out in Costa Rica during the period 1966 - 2010. Methods: Bibliometric analysis of the publications compiled in the serial named “Research publications on drug abuse and drug dependence” which is published by the Costa Rican’s National Institute on Alcoholism and Drug Dependence since 1990. Suchserial has been created using an instrument which allows gathering some bibliometric characteristics of interest in libraries’ catalogues and data bases located in different local institutions. Results: 491 publications have been studied, one third of these publications were published in scientific journals while 33% and 6% were published as monographs and books. About one third of the publications correspond to epidemiologic research. The drug mostly explored was alcohol, which has been studied in the general population as well as in drug affected subjects and in youth and childhood. Discussion: The roll of social sciences on drugs’ knowledge field, the importance and need of a growing feminine participation and the use of qualitative paradigms as well as experimental and quasi experimental research are pointed out and their relevance discussed.
Assuntos
Humanos , Preparações Farmacêuticas , Bibliometria , Transtornos Relacionados ao Uso de Substâncias , Costa Rica , AlcoolismoRESUMO
El propósito de la investigación es analizar si existe estigma y discriminación en las trabajadoras del sexo que consultan la UCSF-E de San Salvador. Materiales y Métodos: Se utilizó un estudio, descriptivo con enfoque cualitativo. Unidad de análisis fueron las trabajadoras del sexo. Se entrevistaron a 18 trabajadoras del sexo. Se realizaron entrevistas a profundidad y una historia de vida. Resultados: En cuanto al nivel de confianza de las trabajadoras del sexo si lo percibieron hacia los prestadores de servicio de dichas clínicas. En cuanto a la atención coincidieron que no se sintieron desvirtuadas la atención recibida fue justa con los tiempos de espera establecidos y por el orden de llegada dentro de la Clínica VICITS. Conclusión: Aunque hay un consenso acerca de la existencia y formas de discriminación hacia dicha población en general se evidencia que se ha reducido considerablemente el nivel de discriminación relacionada a este tipo de pacientes en los servicios de salud en donde se ubican las clínicas VICITS.
Assuntos
Infecções Sexualmente Transmissíveis , Discriminação Social , Saúde PúblicaRESUMO
Las lesiones pigmentadas de la piel y la mucosa bucal constituyen alteraciones que aparecen frecuentemente en la clínica estomatológica; por sus características y las diferentes formas de presentarse, su diagnóstico clínico con frecuencia discrepa del estudio morfológico. Por la importancia clínica de algunas de estas lesiones, nos propusimos hacer un estudio retrospectivo desde el punto de vista clínico y morfológico en 6 años (1998-2003), en los archivos de biopsias del Departamento de Patología de la Facultad de Estomatología. La búsqueda estuvo dirigida a aquellas solicitudes de biopsias sobre lesiones pigmentadas buco-faciales con su correspondiente informe histopatológico, con el propósito de determinar algunos parámetros clínicos como edad, sexo, localización y la correlación de diagnóstico clínico histopatológico. Se seleccionó un total de 155 biopsias de pacientes afectados. Los resultados indicaron que las lesiones que predominaron en los diagnósticos fueron las siguientes: los nevus (de localización cutánea), la pigmentación exógena, el léntigo y el melanoma, con una distribución del 65,2 por ciento, 29 por ciento, 4,5 por ciento y 1,3 por ciento, respectivamente. El sexo femenino fue el más afectado en cada una de las entidades. La presentación según grupos de edades fue variable, se observó que los nevus y el léntigo fueron más frecuentes en los jóvenes, entre los 30 y 39 años de edad (25,7 y 28,6 por ciento), respectivamente), mientras que las pigmentaciones exógenas fueron más frecuentes en pacientes mayores de 60 años (26,7 por ciento) en la cavidad bucal. Se estableció una correlación clínico-histopatológica, y de esta forma se comprobó el diagnóstico clínico o se rectificó este en cada uno de los casos estudiados(AU)
The pigmented injuries of the skin and oral mucosa are alterations that appear commonly at the dental clinic Due to their characteristics and different forms of presentation, their clinical diagnosis usually differs from the morphological study. Taking into consideration the clinical importance of some of these injuries, we proposed ourselves to make a retrospective review of the biopsy archives of the Pathology Department of the Stomatology Faculty from the clinical and morphological point of view during a period of 6 years (1998-2003). The search was directed to those biopsy indications of buccofacial pigmented injuries with their corresponding histopathological report in order to determine some clinical parameters, such as age, sex, localization and the correlation of the clinical histopathological diagnosis. A total of 155 biopsies of patients affected was selected. The results showed that the injuries predominating in the diagnosis were the nevuses (of cutaneous localization), the exogenous pigmentation, the lentigo and the melanoma with a distribution of 65.2 percent, 29 percent, 4.5 percent and 1.3 percent, respectively. Females were the most affected in each of the entities. The presentation according to age groups was variable. It was observed that the nevuses and the lentigo were more frequent among the young individuals aged 30-39 (25.7 percent and 28.6 percent, respectively), whereas the exogenous pigmentations were more common in the oral cavity of patients over 60 (26.7 percent). A clinicohistopathological correlation was established and, this way, the clinical diagnosis was confirmed or rectified in every of the studied cases(AU)
Assuntos
Humanos , Feminino , Adulto , Neoplasias Bucais/etiologia , Lentigo/diagnóstico , Melanoma/diagnóstico , Nevo Pigmentado/diagnóstico , Neoplasias Cutâneas/patologiaRESUMO
El presente trabajo es un estudio retrospectivo sobre Embarazo Ectópico (EE) en el Hospital Gineco-Obstétrico Isidro Ayora (HGIOA) durante los años 1992 y 1993. Encontramos un total de 93 casos con una prevalencia de 1 por 250 nacidos vivos. las pacientes fueron multigestas en el 93.5 por ciento de casos y la media de la edad materna fue de 27.9 años. La media de la edad gestacional fue 7 semanas. El factor predisponente más importante, el aborto anterior al EE 54,8 por ciento el cuadro clínico fue dominado por dolor hipogástrico, sangrado genital y anexo doloroso con defensa abdominal a la palpación. Para confirmar el diagnóstico se utilizó el ECO pélvico y culdocentesis. El mayor porcentaje de EE se localizan en la ampolla tubárica 62 por ciento. El 89.2 por ciento de pacientes acudieron al servicio de urgencias de este hospital con EE roto, realizandose salpingectomia total en el 80.6 por ciento de los casos.