1.
Chembiochem
; 12(18): 2753-8, 2011 Dec 16.
Artigo
em Inglês
| MEDLINE
| ID: mdl-22021215
RESUMO
Messenger bagged: The design of a fluorophore-labeled protein biosensor for the bacterial messenger cyclic di-GMP is described. The biosensor responds to c-di-GMP with sub-micromolar sensitivity in a real-time fashion. The biosensor can be used for enzyme assays for diguanylate cyclases and c-di-GMP phosphodiesterases as well as the high-throughput screening of inhibitors.