Biodegradation of high molecular weight polycyclic aromatic hydrocarbons by Sarocladium terricola strain PYR-233 isolated from petrochemical contaminated sediment.

Polycyclic aromatic hydrocarbons (PAHs) were frequently found in sediment and were primarily treated through microbial degradation. Thus, efficient management of PAH pollution requires exploring the molecular degradation mechanisms of PAHs and expanding the pool of available microbial resources. A fungus (identified as Sarocladium terricola strain RCEF778) with the remarkable ability to degrade pyrene was screened from sediment near a petrochemical plant, and its growth and pyrene degradation characteristics were comprehensively investigated. The results showed that the fungus exhibited great effectiveness in pyrene degradation, with a degradation ratio of 88.97% at 21 days at the conditions: 35 °C, pH 7, 10 mg L-1 initially pyrene concentration, 3% supplementary salt, and glucose supplementation. The generation and concentration variation of the intermediate products were identified, and the results revealed that the fungus degraded pyrene through two pathways: by salicylic acid and by phthalic acid. Three sediments (M1, M2, M3), each exhibiting different levels of PAH pollution, were employed to examine the effectiveness of fungal degradation of PAHs in practical sediment samples. These data showed that with the fungus, the degradation ratios ranged from 13.64% to 23.50% for 2-3 rings PAHs, 40.93%-49.41% for 4 rings PAHs, and 39.59%-48.07% for 5-6 rings PAHs, which were significantly higher than those for the sediment without the fungus and confirmed the excellent performance of the fungal. Moreover, the Gompertz model was employed to analyze the degradation kinetics of 4-rings and 5-6 rings PAHs in these sediments, and the results demonstrated that the addition of the fungus could significantly increase the maximum degradation ratio, degradation start-up rate and maximum degradation rate of 4-rings and 5-6 rings PAHs and shorten the time required to reach the maximum degradation rate. This study not only supplied fungal materials but also established crucial theoretical foundations for the development of bioremediation technologies aimed at high molecular weight PAH-contaminated sediments.

Research progress and perspective on sludge anaerobic digestion technology: A bibliometric analysis.

Rational disposal of sludge is an ongoing concern. This work is the first attempt for in-depth statistical analysis of anaerobic digestion (AD) research in recent three decades (1986-2022) using both quantitative and qualitative approaches in bibliometrics to investigate the research progress, trends and hot spots. All publications in the Web of Science Core Collection database from 1986 to April 4, 2022 were analyzed. Results showed that the research on AD started in 1999 and the number of papers significantly increased since 2012. The research about the disposal of sewage sludge mainly focuses on energy recovery (e.g. methane and short chain volatile organic acids) by AD. Besides, different pretreatment technologies were studied in this study to eliminate the negative effects on the disposal of sludge caused by hydrolysis (rate-limiting step of AD), water content (increasing the costs) and heavy metal (toxic to the environment) of sludge. Of those, the treatment technologies related to direct interspecies electron transfer were worth further studied in the future. Towards that end, iron conductive material, iron-based advanced oxidation and biological treatment were concluded as the prospective technologies and worth to further study.

Biotransformation and detoxification mechanism of inorganic arsenic in a freshwater benthic fish Tachysurus fulvidraco with dietborne exposure.

Inorganic arsenic (iAs) is a pervasive environmental toxin, its metabolism and detoxification mechanism in freshwater benthic fish under dietary exposure remain unknown. In this study, dietborne exposure of two iAs (arsenate (AsV) and arsenite (AsIII)) was conducted for yellow catfish (Tachysurus fulvidraco) to investigate the bioaccumulation, biotransformation, and detoxification of iAs in the fish liver. The results showed that As significantly accumulated in both the AsIII and AsV treatments compared to the control, and the final As concentration was comparable for both treatments. The detoxification of iAs in freshwater fish depends on the degree of arsenic methylation and the level of antioxidants. Both reduction processes of AsV to AsIII and oxidation processes of AsIII to AsV were found in AsV and AsIII treatments. The major-low toxicity intermediates, which also are detoxification products in the AsIII treatment, were pentavalent dimethylarsinic acid and arsenobetaine (AsB), and AsB was major-low toxicity intermediate in the AsV treatment. Both antioxidants glutathione and glutathione S-transferase contribute to the detoxification of iAs by scavenging excessive reactive oxygen species and promoting iAs methylation in yellow catfish under iAs exposure.

The crucial elements for lettuce (Lactuca sativa L.) growth under DMA stress and the linkage with DMA behavior: A new application of ionome.

Dimethylarsinic acid (DMA) is one of the common arsenic (As) species present in soil and is more toxic to plants than others. Identifying the crucial elements for plant growth under DMA stress is essential to enhance plant tolerance to DMA. Herein, we provided for the first time an ionome-based approach to address this issue. The phenotype, As species and concentrations of 11 essential elements in lettuce tissues were monitored under exposures of 0.1, 0.5, 1, 2, 5 mg L-1 DMA in hydroponic culture for 32 days. Lettuces remained normal (no significant difference in phenotype from the control) under 0.1-2 mg L-1 DMA stress, and were inhibited with fresh weights of leaf and root under 5 mg L-1 DMA stress. Integrating the difference in ionome profiles between the two growth states (normal and inhibited) and the responses of the individual element, Mg and S were clarified as the most possible candidates for the crucial elements for lettuce growth under DMA stress. Under 5 mg L-1 DMA stress, the accumulation of Mg and S declined, yet their BCF values were significantly increased, which was consistent with the change in BCF of DMA. Based on the physiological functions of Mg and S and the toxicity of DMA, it could be inferred that the enhanced transfer of Mg and S to leaves should be induced by the potential damage caused by the increased DMA accumulation in leaves, and would result in a shortage of both elements in roots as well as the growth inhibition.

Integrated comparison of growth and oxidative stress induced by tylosin in two freshwater algae Chlorella vulgaris and Raphidocelis subcapitata.

Two model algae, Chlorella vulgaris (C. vulgaris) and Raphidocelis subcapitata (R. subcapitata), are commonly used in registration procedures to evaluate compounds with antimicrobial capacity. However, it has been found that these two algae show considerable differences in sensitivity when exposed to antibiotics. The selection of a suitable test species plays a crucial role in assessing the environmental hazards and risks of a compound, as the balance between oxidative stress and antioxidants is a key factor for alga growth. This study was conducted to investigate the status of oxidative stress and mechanism of antioxidant defense system of algae under antibiotic stress. Different tylosin (TYN) exposure-concentrations were used for the tests in this study. Oxidative stress biomarkers (malondialdehyde (MDA)), non-enzymatic antioxidants (reduced glutathione (GSH)), antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GP), glutathione S-transferase (GST)) and photosynthetic pigments were measured to determine the status of the antioxidant defense system. With increasing TYN concentration, the growth of R. subcapitata was significantly inhibited, while there was no effect on C. vulgaris. When the growth of R. subcapitata was inhibited, the content of MDA was significantly increased and the antioxidant system was activated, which indicated a significant increase in the activity of SOD and CAT.

Analysis of the potential of reclaimed water utilization in typical inland cities in northwest China via system dynamics.

Located in the hinterland of the Eurasian continent, the inland areas of northwestern China have an arid climate and are confronted with continuing challenges in water resources quantity and quality. Reclaimed water plays an important role in maintaining the sustainable use of urban water resources, especially in arid regions. In this paper, a system dynamics urban reclaimed water model (SDURWM) considering the status of arid regions in northwestern China was constructed. The supply and demand balance index (RWB) and the utilization efficiency index (RWUE) of reclaimed water were proposed as evaluation indicators. The potential of reclaimed water utilization (2016-2025) in three typical cities with similar natural environments and population sizes but with large economic differences were estimated by the SD model. Then the relevant values of reclaimed water quota, supply and resident awareness were adjusted within the range of 10%-20%. The predicted results from intervention demonstrate that the RWB of three cities will be closer or maintain to the ideal value, and that the RWUE will increase by about 0.1, compared with the non-intervention results. This indicates that a minor change of the targeted policies can invigorate the capacity of reclaimed water use in droughty cities. This paper provides some effective solutions for the government's planning of developing the potential of reclaimed water.

Degradation of pyrene by Xanthobacteraceae bacterium strain S3 isolated from the rhizosphere sediment of Vallisneria natans: active conditions, metabolite identification, and proposed pathways.

Polycyclic aromatic hydrocarbons (PAHs) were typical environmental contaminants that accumulated continuously in sediment. Microbial degradation is the main way of PAH degradation in the natural environment. Therefore, expanding the available pool of microbial resources and investigating the molecular degrading mechanisms of PAHs are critical to the efficient control of PAH-polluted sites. Here, a strain (identified as Xanthobacteraceae bacterium) with the ability to degrade pyrene was screened from the rhizosphere sediment of Vallisneria natans. Response surface analysis showed that the strain could degrade pyrene at pH 5-7, NaCl addition 0-1.5%, and temperature 25-40 °C, and the maximum pyrene degradation (~ 95.4%) was obtained under the optimum conditions (pH 7.0, temperature 28.5 °C, and NaCl-free addition) after 72 h. Also, it was observed that the effect of temperature on the degradation ratio was the most significant. Furthermore, eighteen metabolites were identified by mass spectrometry, among which (2Z)-2-hydroxy-3-(4-oxo-4H-phenalen-3-yl) prop-2-enoic acid, 7-(carboxymethyl)-8-formyl-1-naphthyl acetic acid, phthalic acid, naphthalene-1,2-diol, and phenol were the main metabolites. And the degradation pathway of pyrene was proposed, suggesting that pyrene undergoes initial ortho-cleavage under the catalysis of metapyrocatechase to form (2Z)-2-hydroxy-3-(4-oxo-4H-phenalen-3-yl) prop-2-enoic acid. Subsequently, this intermediate was progressively oxidized and degraded to phthalic acid or phenol, which could enter the tricarboxylic acid cycle. Furthermore, the pyrene biodegradation by the strain followed the first-order kinetic model and the degradation rate changed from fast to slow, with the rate remaining mostly slow in the later stages. The slow biodegradation rate was probably caused by a significant amount of phenol accumulation in the initial stage of degradation, which resulted in a decrease in bacterial activity or death.

How do polystyrene microplastics affect the adsorption of copper in soil?

Microplastics (MPs) commonly coexist with heavy metals in the soil environment. MPs can influence the activity of heavy metals, and the specific mechanisms need to be further explored. Here, different contents of polystyrene (PS) MPs were added to soil to explore their effects on the adsorption and desorption characteristics of copper (Cu2+) in soil. The adsorption process was mainly chemical adsorption and belonged to a spontaneous, endothermic reaction. The hydrophobicity of MPs slowed down the adsorption and desorption rates. The main adsorption mechanisms included complexation by oxygen-containing functional groups, ion exchange (accounting for 33.97-36.04 % of the total adsorption amounts), and electrostatic interactions. MPs lacked oxygen-containing functional groups and were predominantly engaged in ion exchange and electrostatic interactions. MPs diluted, blocked the soil, and covered the active sites of soil, which reduced adsorption (3.56-16.18 %) and increased desorption (0.90-2.07 %) of Cu2+ in soil samples, thus increasing the activity and mobility of Cu2+. These findings provide new insights into the effects of MPs on the fate and risk of heavy metals in soil. ENVIRONMENTAL IMPLICATION: The existing literature concerning the effects of microplastics on the adsorption of heavy metals in soil is insufficient. Our investigation unveiled that the main adsorption mechanisms of different soil samples included complexation by oxygen-containing functional groups, ion exchange (accounting for 33.97-36.04 % of the total adsorption amounts), and electrostatic interactions. MPs lacked oxygen-containing functional groups and were predominantly engaged in ion exchange and electrostatic interactions. MPs diluted, blocked the soil, and covered the active sites of soil, which reduced adsorption (3.56-16.18 %) and increased desorption (0.90-2.07 %) of Cu2+ in soil samples, thus increasing the activity and mobility of Cu2+.

Tylosin toxicity in the alga Raphidocelis subcapitata revealed by integrated analyses of transcriptome and metabolome: Photosynthesis and DNA replication-coupled repair.

Tylosin (TYN) is widely used in veterinary prophylactic as a macrolide and frequently detected in the surface water. Previous studies showed that exposure to TYN caused suppression of chlorophyll biosynthesis and inhibition of photosynthesis at the physiological level, associated with reduced growth performances in algae, but the molecular mechanisms remain unknown, especially at environmental exposure levels. The present study elucidated the underlying molecular mechanism(s) of TYN toxicity in a model green alga Raphidocelis subcapitata using approaches of transcriptomics and metabolomics. Following a 7-day exposure, algal growth performances were reduced by 26.3% and 58.3% in the 3 (an environmentally realistic level) and 400 µg L-1 TYN treatment group, respectively. A total of 577 (99) and 5438 (180) differentially expressed genes (differentially accumulated metabolites) were identified in algae treated with 3 and 400 µg L-1 TYN, respectively. Signaling pathways including photosynthesis - antenna protein, porphyrin and chlorophyll metabolism, carbon fixation in photosynthetic organisms, and DNA replication were altered in the 400 µg L-1 TYN treatment, while photosynthesis and DNA replication were the shared pathways in both TYN treatments. The metabolomic data further suggest that molecular pathways related to photosynthesis, DNA replication-coupled repair and energy metabolism were impaired. Photosynthesis was identified as the most sensitive target of TYN toxicity in R. subcapitata, in contrast to protein synthesis inhibition caused by TYN in bacteria. This study provides novel mechanistic information of TYN toxicity in R. subcapitata.