Room-Temperature Synthesis of Zeolite Membranes toward Optimized Microstructure and Enhanced Butane Isomer Separation Performance.

Room temperature (RT) synthesis of high-performance zeolite membranes, which is profound from techno-economic and eco-friendly perspectives, remains a grand challenge. In this work, we pioneered the RT preparation of well-intergrown pure-silica MFI zeolite (Si-MFI) membranes, which was realized through adopting highly reactive NH4F-mediated gel as nutrient during epitaxial growth. Benefiting from the introduction of fluoride anions as mineralizing agent as well as precisely tuned nucleation and growth kinetics at RT, both their grain boundary structure and thickness could be deliberately controlled, resulting in the formation of Si-MFI membranes showing unprecedented n-/i-butane separation factor (96.7) and n-butane permeance (5.16 × 10-7 mol m-2 s-1 Pa-1) in the case of a feed molar ratio of 10/90, which well transcended the state-of-the-art membranes reported in the literature. This RT synthetic protocol was also proven effective for preparing highly b-oriented Si-MFI film, thus showing great promise for the preparation of diverse zeolite membranes with optimized microstructure and superior performance.

Genome Sequence Resource of 'Candidatus Liberibacter asiaticus' from Diaphorina citri Kuwayama (Hemiptera: Liviidae) in Colombia.

'Candidatus Liberibacter asiaticus' (CLas) is an unculturable phloem-restricted α-proteobacterium associated with huanglongbing (HLB). Here, we provide the genome sequence of CLas strain CoFLP1 from its insect vector Diaphorina citri (Hemiptera: Liviidae) collected in the department of La Guajira, Colombia. The CoFLP1 strain is composed of 1,231,639 bp with G+C 36.5% content. This study reports the first CLas genome sequence from Colombia, which will add to CLas genome resources and help to elucidate our understanding of the introduction pathway of HLB in South America.

Genome Sequence Resource of 'Candidatus Liberibacter asiaticus' from Diaphorina citri Kuwayama (Hemiptera: Liviidae) from La Réunion.

'Candidatus Liberibacter asiaticus' is an insect-transmitted, phloem-restricted α-proteobacterium associated with huanglongbing. Here, we provide the whole genome sequence of 'Ca. L. asiaticus' strain ReuSP1 from its insect vector Diaphorina citri (Hemiptera: Liviidae) collected in La Réunion. The genome is composed of 1,230,064 bp and has a 36.5% G+C content. This study reports the first 'Ca. L. asiaticus' genome sequence from La Réunion, which will add to 'Ca. L. asiaticus' genome resources and help elucidate our understanding of the introduction pathway into La Réunion.

Hierarchy Control of MFI Zeolite Membrane towards Superior Butane Isomer Separation Performance.

Microstructural optimization (such as thickness and preferred orientation) is a major concern for performance enhancement of zeolite membranes. In this study, we demonstrated that the introduction of hierarchy easily enabled concurrent thickness reduction and orientation control of zeolite membranes. Specifically, hierarchical MFI zeolite membranes comprising higher degree of (h0h) preferentially oriented ultrathin (ca. 390 nm) selective top layers and porous intermediate layers on porous α-Al2 O3 substrates were fabricated. The use of hollow-structured MFI nanoseeds and the employment of single-mode microwave heating during membrane processing were found indispensable for the preparation of MFI zeolite membranes with superior butane isomer separation performance, thereby surpassing the current n-/i-butane selectivity versus n-butane permeance trade-off limits of MFI zeolite membranes prepared via solution-based synthetic protocols.

Molecular phylogenetic analysis of the Amiota taurusata species group within the Chinese species, with descriptions of two new species.

The relationships among six species of the Amiota taurusata Takada, Beppu, & Toda (Diptera: Drosophilidae) species group were investigated based on DNA sequence data of the mitochondrial NADH dehydrogenase subunit 2 ( ND2) gene, using three species of the genus Amiota as outgroups. A mitochondrial gene, cytochrome c oxidase I ( COI), can be used to discriminate between species of the taurusata group. Two new species are described from South China: A. protuberantis Shao et Chen, sp. nov. and A. shennongi Shao et Chen, sp. nov. A key to all the species of the taurusata group based on morphological characters is provided.

Dextran sulfate acting as a chaperone-like component on inhibition of amorphous aggregation and enhancing thermal stability of ovotransferrin.

The tendency of ovotransferrin (OVT) to unfold and aggregate under 60 °C severely restricted sterilization temperature during egg processing. Searching for efficient strategies to improve OVT thermal stability is essential for improving egg product quality and processing suitability. Here, we investigated the effect of sulfate polysaccharide (dextran sulfate, DS) on heat-induced aggregation of OVT. We found that DS can effectively suppress amorphous aggregation of OVT at pH 7.0 after heating. Strikingly, the addition of 5 µM DS fully suppressed insoluble aggregates formation of 0.5 mg/mL OVT. Structure analysis confirmed that DS preserves nearly the entire secondary and tertiary structure of OVT during heating. The steric hindrance effect arising from strong electrostatic interactions between OVT and DS, coupled with reduced OVT hydrophobicity, is the underlying mechanism in suppressing protein-protein interactions, thus enhancing thermal stability. These findings suggest DS could act as protein stabilizers and chaperones, enhancing the thermostability of heat-sensitive proteins.

Characterization of two Bursicon genes and their association with wing development in the brown citrus aphid, Aphis citricidus.

The tanning hormone, Bursicon, is a neuropeptide secreted by the insect nervous system that functions as a heterodimer composed of Burs-α and Burs-ß subunits. It plays a critical role in the processes of cuticle tanning and wing expansion in insects. In this study, we successfully identified the AcBurs-α and AcBurs-ß genes in Aphis citricidus. The open reading frames of AcBurs-α and AcBurs-ß were 480 and 417 bp in length, respectively. Both AcBurs-α and AcBurs-ß exhibited 11 conserved cysteine residues. AcBurs-α and AcBurs-ß were expressed during all developmental stages of A. citricidus and showed high expression levels in the winged aphids. To investigate the potential role of AcBurs-α and AcBurs-ß in wing development, we employed RNA interference (RNAi) techniques. With the efficient silencing of AcBurs-α (44.90%) and AcBurs-ß (52.31%), malformed wings were induced in aphids. The proportions of malformed wings were 22.50%, 25.84%, and 38.34% in dsAcBurs-α-, dsAcBur-ß-, and dsAcBurs-α + dsAcBur-ß-treated groups, respectively. Moreover, feeding protein kinase A inhibitors (H-89) also increased the proportion of malformed wings to 30.00%. Feeding both double-stranded RNA and inhibitors (H-89) significantly downregulated the wing development-related genes nubbin, vestigial, notch and spalt major. Silence of vestigial through RNAi also led to malformed wings. Meanwhile, the exogenous application of 3 hormones that influence wing development did not affect the expression level of AcBursicon genes. These findings indicate that AcBursicon genes plays a crucial role in wing development in A. citricidus; therefore, it represents a potential molecular target for the control of this pest through RNAi-based approaches.

Phylogenetic relationship among East Asian species of the Stegana genus group (Diptera, Drosophilidae).

The phylogenetic relationship among 27 East Asian species of the Stegana genus group was reconstructed using DNA sequences of mitochondrial (COI and ND2) and nuclear (28S) genes. The results lent support to the current generic/subgeneric taxonomic classification in the genus group with the exceptions of the paraphyly of the genus Parastegana and the subgenus Oxyphortica in the genus Stegana. The ancestral areas and divergence times in the genus group were reconstructed/estimated, and accordingly, the biogeographical history of this important clade was discussed. It was proposed that, the evolution of the plant family Fagaceae, especially Quercus, may have played a certain role in facilitating the diversification of the Stegana genus group.

[Detecting testis- and epididymis-specific methylated promoters in human cell-free seminal DNA by MeDIP-qPCR].

OBJECTIVE: To establish a method of methyl-DNA immunoprecipitation (MeDIP)-real time quantitative PCR (qPCR) for detecting the promoter methylation level in cell-free seminal DNA (cfsDNA). METHODS: We obtained cfsDNA samples from 6 normozoospermia men (the NZ group) and 6 post-vasectomy patients (the PV group), and mixed the samples from different individuals of each group, respectively. Then we made DNA fragments by ultrasonication, separated the methylated DNA fragments by MeDIP, and determined the methylation level of the promoters in cfsDNA by qPCR. RESULTS: The methylation levels of the promoters PRAME, PEG10, MORC1, GML, HOXA5, DNMT3L, SNURF, MSH4, DAZ1 and CLPB were 14.93, 2.64, 0.69, 2.66, 17.50, 21.10, 5.98, 2.28, 13.50 and 3.86%, respectively, in the NZ group, obviously lower than 121.25, 73.62, 16.25, 42.90, 76.74, 112.40, 59.79, 25.85, 91.90 and 64.53% in the PV group. The results of MeDIP-qPCR for the methylation of PRAME, MORC1, GML, HOXA5, DNMT3L, SNURF, MSH4 and DAZ1 were coincident with the results of genome-wide promoter methylation microarray. CONCLUSION: MeDIP-qPCR can quantitatively measure the promoter methylation level in cfsDNA, and effectively determine the testis- and epididymis-specific methylated promoters in human semen.

The genus Leucophenga (Diptera, Drosophilidae), Part I: The abbreviata species group from the Oriental region with morphological and molecular evidence.

A new species group, the abbreviata group is established within the genus Leucophenga based on one known and three new species, all of which are endemic to the Oriental region: L. abbreviata (de Meijere, 1911), L. brevivena sp. nov., L. sujuanae sp. nov. and L. zhenfangae sp. nov. A key to four species of the abbreviata group and the DNA barcoding are provided. Twenty-three mtDNA COI sequences belonging to the above species are analyzed; the molecular data are used as interactive evidence to evaluate the species boundaries defined by the morphological data.

ETA: An Efficient Training Accelerator for DNNs Based on Hardware-Algorithm Co-Optimization.

Recently, the efficient training of deep neural networks (DNNs) on resource-constrained platforms has attracted increasing attention for protecting user privacy. However, it is still a severe challenge since the DNN training involves intensive computations and a large amount of data access. To deal with these issues, in this work, we implement an efficient training accelerator (ETA) on field-programmable gate array (FPGA) by adopting a hardware-algorithm co-optimization approach. A novel training scheme is proposed to effectively train DNNs using 8-bit precision with arbitrary batch sizes, in which a compact but powerful data format and a hardware-oriented normalization layer are introduced. Thus the computational complexity and memory accesses are significantly reduced. In the ETA, a reconfigurable processing element (PE) is designed to support various computational patterns during training while avoiding redundant calculations from nonunit-stride convolutional layers. With a flexible network-on-chip (NoC) and a hierarchical PE array, computational parallelism and data reuse can be fully exploited, and memory accesses are further reduced. In addition, a unified computing core is developed to execute auxiliary layers such as normalization and weight update (WU), which works in a time-multiplexed manner and consumes only a small amount of hardware resources. The experiments show that our training scheme achieves the state-of-the-art accuracy across multiple models, including CIFAR-VGG16, CIFAR-ResNet20, CIFAR-InceptionV3, ResNet18, and ResNet50. Evaluated on three networks (CIFAR-VGG16, CIFAR-ResNet20, and ResNet18), our ETA on Xilinx VC709 FPGA achieves 610.98, 658.64, and 811.24 GOPS in terms of throughput, respectively. Compared with the prior art, our design demonstrates a speedup of 3.65× and an energy efficiency improvement of 8.54× on CIFAR-ResNet20.

Discovery of Brassica Yellows Virus and Porcine Reproductive and Respiratory Syndrome Virus in Diaphorina citri and Changes in Virome Due to Infection with 'Ca. L. asiaticus'.

Detection of new viruses or new virus hosts is essential for the protection of economically important agroecosystems and human health. Increasingly, metatranscriptomic data are being used to facilitate this process. Such data were obtained from adult Asian citrus psyllids (ACP) (Diaphorina citri Kuwayama) that fed solely on mandarin (Citrus ×aurantium L.) plants grafted with buds infected with 'Candidatus Liberibacter asiaticus' (CLas), a phloem-limited bacterium associated with the severe Asian variant of huanglongbing (HLB), the most destructive disease of citrus. Brassica yellows virus (BrYV), the causative agent of yellowing or leafroll symptoms in brassicaceous plants, and its associated RNA (named as BrYVaRNA) were detected in ACP. In addition, the porcine reproductive and respiratory syndrome virus (PRRSV), which affects pigs and is economically important to pig production, was also found in ACP. These viruses were not detected in insects feeding on plants grafted with CLas-free buds. Changes in the concentrations of insect-specific viruses within the psyllid were caused by coinfection with CLas. IMPORTANCE The cross transmission of pathogenic viruses between different farming systems or plant communities is a major threat to plants and animals and, potentially, human health. The use of metagenomics is an effective approach to discover viruses and vectors. Here, we collected buds from the CLas-infected and CLas-free mandarin (Citrus ×aurantium L. [Rutaceae: Aurantioideae: Aurantieae]) trees from a commercial orchard and grafted them onto CLas-free mandarin plants under laboratory conditions. Through metatranscriptome sequencing, we first identified the Asian citrus psyllids feeding on plants grafted with CLas-infected buds carried the plant pathogen, brassica yellows virus and its associated RNA, and the swine pathogen, porcine reproductive and respiratory syndrome virus. These discoveries indicate that both viruses can be transmitted by grafting and acquired by ACP from CLas+ mandarin seedlings.

Facile Synthesis of Ultra-microporous Pillar-Layered Metal-Organic Framework Membranes for Highly H2-Selective Separation.

Recently, pillar-layered MOF materials have attracted much attention and shown great potential in separation application due to their fine pore size/channel and pore surface chemistry tunability and designability. In this work, we reported an effective and universal synthesis strategy for preparing ultra-microporous Ni-based pillar-layered MOF [Ni2(L-asp)2(bpy)] (Ni-LAB) and [Ni2(L-asp)2(pz)] (Ni-LAP) (L-asp = L-aspartic acid, bpy = 4,4'-bipyridine, pz = pyrazine) membranes on a porous α-Al2O3 substrate with high performance and good stability by secondary growth. Through this strategy, the seed size reduction and screening engineering (SRSE) is proposed to obtain uniform sub-micron size MOF seeds by high-energy ball milling-combined solvent deposition. This strategy not only effectively addresses the issue of obtaining the uniform small seeds being significant for secondary growth but also provides an approach for the preparation of Ni-based pillar-layered MOF membranes where the freedom of synthesizing small crystals is lacking. Based on reticular chemistry, the pore size of Ni-LAB was narrowed by making use of shorter pillar ligands of pz instead of the longer pillar ligand of bpy. The prepared ultra-microporous Ni-LAP membranes exhibited a high H2/CO2 separation factor of 40.4 with H2 permeance of 9.69 × 10-8 mol m-2 s-1 Pa-1 under ambient conditions and good mechanical and thermal stability. The superiority of the tunable pore structure and the remarkable stability of these MOF materials showed great potential for industrial H2 purification. More importantly, our synthesis strategy demonstrated the generality for preparation of MOF membranes, enabling the regulation of membrane pore size and surface functional groups by reticular chemistry.

BAICALEIN RELIEVES BRAIN INJURY VIA INHIBITING FERROPTOSIS AND ENDOPLASMIC RETICULUM STRESS IN A RAT MODEL OF CARDIAC ARREST.

ABSTRACT: Background: Cardiac arrest (CA) is one of the leading causes of death worldwide. Endoplasmic reticulum (ER) stress and ferroptosis are proven pathological mechanisms implicated in neuronal damage. Baicalein, a ferroptosis Inhibitor, improved outcomes after traumatic brain injury. We aimed to explore the effects of baicalein on brain injury via ferroptosis and ER stress in a rat model of CA.Methods: Cardiac arrest models were established in Sprague-Dawley (SD) rats. The sham group (n = 6) was untreated with inducing ventricular fibrillation to cardiac arrest and cardiopulmonary resuscitation (CPR). Survival rats were randomly divided into five groups (n = 6). Ferroptosis inhibitor and ER stress agonist were administered separately and together in three groups. There was no drug intervention in the remaining group. The neurological deficit scores were recorded. Characteristics of ferroptosis were observed. And the associated protein of ferroptosis and ER stress were determined by Western blot. Cerebral ROS production was measured by using 2',7'-dichlorofluorescein diacetate as the oxidative fluorescent probe. Results: Baicalein treatment improved neurological outcomes and decreased neurocyte injuries compared with CPR group. The changes of ferroptosis, more specifically, iron content, glutathione peroxidase 4 (GPX4), reactive oxygen species (ROS), arachidonate 15-lipoxygenase (ALOX15) and mitochondrial characteristics, were observed in brain tissue after ROSC. ALOX15 was lower in baicalein group than in CPR group. The morphology and structure of mitochondria in baicalein group were better than in CPR group. The ER stress markers, glucose-regulated protein 78, activating Transcription Factor 4 and C/EBP homologous protein was lower in baicalein group compared with CPR group. ROS in tunicamycin group was higher than in CPR group. And ROS in baicalein +tunicamycin group was lower than in tunicamycin group. Conclusion: Ferroptosis and ER stress are both involved in brain injury after ROSC. Baicalein alleviates brain injury via suppressing the ferroptosis and ER stress, and reduces ROS partly through inhibiting ER stress. Baicalein is a potential drug to relieve brain injury after ROSC.

Characterization and transcriptional expression of ABCG genes in Bactrocera dorsalis: Insights into their roles in fecundity and insecticidal stress response.

The ATP-binding cassette (ABC) transporters are essential for regulating various physiological processes and insecticide resistance across different living organisms. ABCG subfamily genes have diverse functions in insects, but little is known about the function of ABCGs in a serious agricultural pest, Bactrocera dorsalis. In this study, 15 BdABCG genes were identified, and BdABCG6 and BdABCG11 were highly expressed in the pupal and adult stages, especially during the transition period from pupae to adults. Silencing of these two genes resulted in a significant reduction of egg production in B. dorsalis, confirming their importance in reproduction. Analysis of tissue expression patterns showed that most genes, including BdABCG1, 3, 8, and 14, exhibited tissue-specificity, with significantly higher expression levels observed in the intestine, Malpighian tubule, and fat body compared to other tissues. Meanwhile, the induction of malathion and avermectin can significantly upregulate the expression of the above four genes. Furthermore, knockdown of BdABCG3 by RNAi significantly increased the mortality of B. dorsalis upon exposure to avermectin, which suggested that BdABCG3 is involved in the transport or metabolism of avermectin in B. dorsalis. Overall, our work provides valuable insights into the function of BdABCGs involved in the reproduction and detoxification system of B. dorsalis.

Inorganic Pillar Center-Facilitated Counterdiffusion Synthesis for Highly H2 Perm-Selective KAUST-7 Membranes.

Fluorinated metal-organic framework materials (NbOFFIVE-1-Ni, also referred to as KAUST-7) have attracted widespread attention because of their high chemical stability and thermal stability, outstanding tolerance with water and H2S, and high CO2-adsorption selectivity over H2 and CH4. KAUST-7 was expected to be a new membrane material candidate for H2/CO2 separation because of the hindered permeation of CO2 resulting from the interaction between CO2 and (NbOF5)2- of the KAUST-7 framework. A highly H2 perm-selective KAUST-7 membrane was first achieved using a novel strategy of inorganic pillar center-facilitated counterdiffusion (IPCFCD) proposed by us. The IPCFCD method not only effectively avoided the corrosion of hydrofluoric acid to α-Al2O3 tubes in the process of preparing KAUST-7 membranes, but also better reduced grain boundary defects because of the faster nucleation rate and resultant high crystallinity. The KAUST-7 membrane exhibited a high H2/CO2 separation factor (SF) of 27.30 for the 1:1 H2/CO2 binary gas mixture with a high H2 permeance of 5.30 × 10-7 mol m-2 s-1 Pa-1 under ambient conditions and a slight decrease of the H2/CO2 SF with increasing operation temperature and presence of steam. This study highlighted the importance of pre-synthesizing inorganic pillar centers (NiNbOF5 intermediate) and the innovation of a membrane formation process for synthesizing polycrystalline KAUST-7 membranes. Most important of all, our study provided a novel approach to overcome the challenge in fabricating metal-organic framework membranes containing corrosive reactants for the corresponding supports.

Acute transient thyroid swelling after fine-needle aspiration biopsy: A case report of a rare complication and a literature review.

Fine-needle aspiration biopsy (FNAB) is a safe and effective thyroid examination method with rare complications. Herein, we report a rare case of acute transient thyroid swelling that occurred after ultrasound-guided FNAB. The patient experienced acute pain with rapid thyroid swelling. Ultrasound imaging revealed a nodule with a linear, hypoechoic, and "patch-like" appearance, indicating edema without hemorrhage. After receiving anti-anaphylaxis and detumescence therapy for 1 day, the swelling regressed. Acute transient thyroid swelling is an extremely rare event that occurs shortly after FNAB and may frighten patients; therefore, clinicians should be aware of this complication in this context.

Electrically detected electron-spin-echo envelope modulation: a highly sensitive technique for resolving complex interface structures.

We show that the electrical detection of electron-spin-echo envelope modulation (ESEEM) is a highly sensitive tool to study interfaces. Taking the Si/SiO2 interface defects in phosphorus-doped crystalline silicon as an example, we find that the main features of the observed echo modulation pattern allow us to develop a microscopic model for the dangling-bond-like P(b0) center by comparison with the results of ab initio calculations. The ESEEM spectrum is found to be far more sensitive to the defect characteristics than the spectrally resolved hyperfine splitting itself.

Molecular phylogenetic analysis of the Stegana ornatipes species group (Diptera: Drosophilidae) in China, with description of a new species.

A new species of the Stegana (Steganina) ornatipes species group (Diptera: Drosophilidae) is described from Hainan, China, S. (S.) xipengi sp. nov. Based on the mitochondrial ND2 and COI gene sequences, the relationships among eight species from mainland China of the ornatipes group, and their relationships to the undulata, nigrolimbata and shirozui species groups of the same subgenus, are investigated, using two species of the subgenus Stegana, S. emeiensis and S. quadrata, as outgroups. The result shows that S. (S.) mengla is debarred from the ornatipes group.

The first complete mitochondrial genome sequence of Cacopsylla citrisuga (Yang & Li), a new insect vector of Huanglongbing in Yunnan Province, China.

Cacopsylla citrisuga (Yang & Li) is an important pest-threatening Citrus and Poncirus plants (Rutaceae) and a newly identified insect vector of citrus Huanglongbing. The complete mitochondrial genome of C. citrisuga was 14,906 bp in length, with 37 genes, including 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs). The phylogenetic trees inferred from Bayesian inference and maximum likelihood analyses confirmed C. citrisuga as a member of the genus Cacopsylla. Our phylogenetic analyses suggested that the Cacopsylla is paraphyletic, and confirmed C. citrisuga as a member of clade-I under Cacopsylla. The complete mitochondrial genome of C. citrisuga will provide important information for the phylogeny and evolution analysis of Cacopsylla.