Circular RNA circ_0124644 exacerbates the ox-LDL-induced endothelial injury in human vascular endothelial cells through regulating PAPP-A by acting as a sponge of miR-149-5p.

The modulatory roles of numerous circular RNAs (circRNAs) have been exposited in atherosclerosis (AS). Our study paid attention to the function of circRNA_ 0124644 (circ_0124644) in AS development, as well as its functional mechanism. The AS cell model was established by the treatment of oxidized low-density lipoprotein (ox-LDL) to human vascular endothelial cells (HUVECs). Cell proliferation and cycle were severally measured by Cell Counting Kit-8 (CCK-8) and cell cycle detection kit. The examination of apoptosis rate was executed through flow cytometry. Western blot was exploited for detecting the associated proteins. The expression levels of circ_0124644 and microRNA-149-5p (miR-149-5p) and pregnancy-associated plasma protein-A (PAPP-A) were assayed using quantitative real-time polymerase chain reaction. The combination of targets was validated via the dual-luciferase reporter assay, RNA immunoprecipitation (RIP), and RNA pull-down assay. Clonal capacity was analyzed using colony formation assay. Ox-LDL restrained HUVECs proliferation and cycle, but facilitated apoptosis. Circ_0124644 expression was increased, while miR-149-5p was downregulated in ox-LDL-treated HUVECs. Besides, circ_0124644 served as a molecular sponge of miR-149-5p and intensified the ox-LDL-induced HUVECs injury by sponging miR-149-5p. PAPP-A was a target of miR-149-5p and miR-149-5p could mitigate the HUVECs injury caused by ox-LDL through inhibiting PAPP-A. Moreover, PAPP-A was positively regulated by circ_0124644 via the miR-149-5p. In this report, we concluded the promoted role of circ_0124644 in the ox-LDL-induced endothelial injury of HUVECs via the miR-149-5p/PAPP-A axis with an emphasis on its diagnostic and therapeutic values in AS.

Behavior and mechanism of the adsorption of lead by an eco-friendly porous double-network hydrogel derived from keratin.

In this study, a novel eco-friendly porous double-network keratin/polyacrylic acid (keratin-PAA) hydrogel was prepared using the one-pot method to improve the adsorption performance of the hydrogel toward Pb(II). The obtained porous keratin-PAA hydrogel was then characterized using nitrogen adsorption-desorption isotherms, thermogravimetric analysis (TGA), and scanning electron microscopy (SEM). The interaction mechanism of Pb(II) and the keratin-PAA hydrogel was further investigated using X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR). The results showed that keratin-PAA hydrogel was successfully synthesized, with a specific surface area of 49.35 m2/g and a uniform pore distribution of 6.20 nm. The synthesized keratin-PAA hydrogel only took 6 min to adsorb nearly 70% of Pb(II) from the solution because of the interconnected porous network. The keratin-PAA hydrogel also showed a maximal adsorption amount of 234.6 mg/g, and satisfactory selectivity toward Pb(II). The adsorption kinetics of the keratin-PAA hydrogel binding to Pb(II) could be better described by the pseudo-second-order model, whereas the adsorption isotherms could be fitted using the Langmuir equation; this suggested that chemisorption was the main rate-limiting step. The XPS and FT-IR analysis results indicated that the sulfur-, nitrogen- and oxygen-containing groups in the keratin-PAA hydrogel were the main binding sites for Pb(II). In real aqueous samples, the keratin-PAA hydrogel could remove 93-104% of Pb(II). It is clear that the keratin-PAA hydrogel is an outstanding adsorbent material for the removal of Pb(II) from aqueous samples.