Unveiling microplastics pollution in a subtropical rural recreational lake: A novel insight.

While global attention has been primarily focused on the occurrence and persistence of microplastics (MP) in urban lakes, relatively little attention has been paid to the problem of MP pollution in rural recreational lakes. This pioneering study aims to shed light on MP size, composition, abundance, spatial distribution, and contributing factors in a rural recreational lake, 'Nikli Lake' in Kishoreganj, Bangladesh. Using density separation, MPs were extracted from 30 water and 30 sediment samples taken from ten different locations in the lake. Subsequent characterization was carried out using a combination of techniques, including a stereomicroscope, Fourier transform infrared spectroscopy (FTIR) and field emission scanning electron microscopy (FE-SEM). The results showed a significant prevalence of MPs in all samples, with an average amount of 109.667 ± 10.892 pieces/kg3 (dw) in the sediment and 98.167 ± 12.849 pieces/m3 in the water. Small MPs (<0.5 mm), fragments and transparent colored particles formed the majority, accounting for 80.2%, 64.5% and 55.3% in water and 78.9%, 66.4% and 64.3% in sediment, respectively. In line with global trends, polypropylene (PP) (53%) and polyethylene (PE) (43%) emerged as the predominant polymers within the MPs. MP contents in water and sediment showed positive correlations with outflow, while they correlated negatively with inflow and lake depth (p > 0.05). Local activities such as the discharge of domestic sewage, fishing waste and agricultural runoff significantly influence the distribution of polypropylene. Assessment of pollution factor, pollution risk index and pollution load index values at the sampling sites confirmed the presence of MPs, with values above 1. This study is a baseline database that provides a comprehensive understanding of MP pollution in the freshwater ecosystem of Bangladesh, particularly in a rural recreational lake. A crucial next step is to explore ecotoxicological mechanisms, legislative measures and future research challenges triggered by MP pollution.

Enhancement of root abscisic acid mediated osmotic regulation by macroalgal compounds promotes adaptability of rice (Oryza sativa L.) in response to progressive metal ion mediated environmental stress.

Soil deterioration is a major cause of poor agricultural productivity, necessitating sufficient nutrient inputs like fertilizers and amendments for sustainable use. As one such strategy, the current study evaluates the potential of Sargassum wightii, a brown seaweed extract, as an osmopriming agent to improve seed germination, early establishment, and competent seedling performances in acidic soil. The elemental makeup of seaweed extract (BS) showed that it included major plant macro (Potassium, Nitrogen and Phosphorous), as well as micronutrients (Magnesium and Iron) and trace elements (Zinc, Copper, and Molybdenum). While seed germination was impacted by H+ ion toxicity, seeds primed with BS emerged earlier and showed a higher germination percentage (98.2%) and energy (92.4%). BS treatments enhanced seedling growth by 63% and had a positive effect on root growth (68.2%) as well as increases in root surface area (10%) and volume (67.01%). Stressed seedlings had 76.39% and 63.2% less carotenoid and chlorophyll, respectively. In seedlings treated with BS, an increase in protein and Total Soluble Sugars content of 14.56 and 7.19%, respectively, was seen. Fourier Transform-Infra Red analysis of postharvest soil indicated improved soil health with absorbance corresponding to enhanced soil water holding capacity and organic matter. Increased abscisic acid synthesis rate and associated antioxidant enzyme system (Malondialdehyde, Glutathione peroxidases and ascorbate peroxidase) activation, along with enhanced H+ adenosine triphosphate-ase and glutathione activities, help ameliorate and deport H+ ions from cells, scavenge Reactive Oxygen Species, thus protecting cells from injury. Seaweed extract successfully reduced H+-induced ion toxicities in rice by promoting their germination, physiological, metabolically, and growth parameters that could ultimately increase their productivity and yield in a sustainable and environmentally friendly manner.

Management of excessive soil H+ ion induced toxicities by application of organic seaweed amendment enhances photosynthesis and resource use efficiencies in rice (Oryza sativa).

Globally, soil acidification is a serious environmental issue that reduces commercial agricultural production. Rice is subjected to nutritional stress due to acidic soil, which is a major impediment to rice production. Since acid soil threatens rice plants with soil compaction, nutrient loss, and plant stress-induced oxidative cell damage that results in affecting the photosynthetic system, restricting the availability of water, and reducing overall plant growth and productivity. Since contemporary soil acidification management strategies provide mediocre results, the use of Sargassum wightii seaweed-based biostimulants (BS) and soil amendments is sought as an environmentally friendly alternative strategy, and therefore its potential isevaluated in this study. BS was able to mediate soil quality by improving soil pH and structure along with facilitating nitrogen phytoavailability. BS also increased the activity of the antioxidant enzyme system, superoxide dismutase ((48%), peroxidase (76.6%), and ascorbate peroxidase (63.5%), aggregating the monaldehyde-mediating accumulation of osmoprotective proline in roots, that was evident from rapid initiation of root hair growth in treated seedlings. BS was also able to physiologically modulate photosynthetic activities and chlorophyll production (24.31%) in leaves, maintaining the efficiency of plant water use by regulating the stomatal conductance (0.91 mol/m/s) and the transpiration rate (13.2 mM/m/s). The BS compounds were also successful in facilitating nitrogen uptake resulting in improved plant growth (59%), tiller-panicle number, and yield (52.57%), demonstrating a resourceful nitrogen use efficiency (71.96%) previously affected by stress induced by acid soil. Therefore, the study affirms the competent potential of S. wightii-based soil amendment to be applied not only to improve soil quality, but also to increase plant production and yield.

Single-cell RNA-seq analysis decodes the kidney microenvironment induced by polystyrene microplastics in mice receiving a high-fat diet.

In recent years, the environmental health issue of microplastics has aroused an increasingly significant concern. Some studies suggested that exposure to polystyrene microplastics (PS-MPs) may lead to renal inflammation and oxidative stress in animals. However, little is known about the essential effects of PS-MPs with high-fat diet (HFD) on renal development and microenvironment. In this study, we provided the single-cell transcriptomic landscape of the kidney microenvironment induced by PS-MPs and HFD in mouse models by unbiased single-cell RNA sequencing (scRNA-seq). The kidney injury cell atlases in mice were evaluated after continued PS-MPs exposure, or HFD treated for 35 days. Results showed that PS-MPs plus HFD treatment aggravated the kidney injury and profibrotic microenvironment, reshaping mouse kidney cellular components. First, we found that PS-MPs plus HFD treatment acted on extracellular matrix organization of renal epithelial cells, specifically the proximal and distal convoluted tubule cells, to inhibit renal development and induce ROS-driven carcinogenesis. Second, PS-MPs plus HFD treatment induced activated PI3K-Akt, MAPK, and IL-17 signaling pathways in endothelial cells. Besides, PS-MPs plus HFD treatment markedly increased the proportions of CD8+ effector T cells and proliferating T cells. Notably, mononuclear phagocytes exhibited substantial remodeling and enriched in oxidative phosphorylation and chemical carcinogenesis pathways after PS-MPs plus HFD treatment, typified by alterations tissue-resident M2-like PF4+ macrophages. Multispectral immunofluorescence and immunohistochemistry identified PF4+ macrophages in clear cell renal cell carcinoma (ccRCC) and adjacent normal tissues, indicating that activate PF4+ macrophages might regulate the profibrotic and pro-tumorigenic microenvironment after renal injury. In conclusion, this study first systematically revealed molecular variation of renal cells and immune cells in mice kidney microenvironment induced by PS-MPs and HFD with the scRNA-seq approach, which provided a molecular basis for decoding the effects of PS-MPs on genitourinary injury and understanding their potential profibrotic and carcinogenesis in mammals.

A mini-review on plasticrusts: occurrence, current trends, potential threats, and recommendations for coastal sustainability.

Plasticrusts manifest as a coating on intertidal rocks due to environmental exposure. They refer to crushed plastic debris that blankets rocks found along intertidal shorelines. This study significantly contributes to a better understanding of the occurrence of these novel plastic formations, shedding light on their potential pathways of formation during the Anthropocene era. The research provides comprehensive insights into the composition, origins, challenges, and effective management strategies for removing coastal plastic litter. The findings of this investigation offer valuable evidence regarding the formation and impact of these recently discovered plastic items in coastal regions, prompting discussions about their formation processes and their effects on the marine ecosystem. Recognizing that these newly emerged plastic litter pose a considerable threat to the marine environment is crucial. With their emergence, we face an environmental challenge, especially concerning the health of coastal ecosystems. Plasticrusts, when degraded, can release microplastics (MPs) and nanoparticles (NPs) into the surrounding environment. These micro- and nano-sized plastic particles pose significant ecological risks as they persist in ecosystems, potentially harming wildlife and entering the food chain, causing widespread environmental contamination. Significantly, it outlines strategies to minimize the impact of this emerging plastic debris and its source.

Seaweed Sargassum wightii mediated preparation of TiO2 nanoparticles, larvicidal activity against malaria and filariasis vectors, and its effect on non-target organisms.

Malaria and Lymphatic filariasis are considered significant public health concerns in several countries. As a researcher, controlling those mosquitos using safe and eco-friendly insecticides is essential. Thus, we aimed to explore the potential use of seaweed Sargassum wightii for the biosynthesis of TiO2 NPs and evaluate its efficiency in controlling disease-transmitting mosquito larvae (using Anopheles subpictus and Culex quinquefasciatus larvae as model systems (in vivo)) as well as its potential effect on non-target organisms (using Poecilia reticulata fish as an experimental model). XRD, FT-IR, SEM-EDAX, and TEM carried out the characterization of TiO2 NPs. It evaluated the larvicidal activity against the fourth instar larvae of A. subpictus and C. quinquefasciatus. The larvicidal mortality was observed after 24 h of exposure to S. wightii extract and TiO2 NPs. S. wightii synthesized TiO2 NPs show excellent activity against A. subpictus and C. quinquefasciatus (LC50 = 4.37 and 4.68; LC90 = 8.33 and 8.97; χ2 = 5.741 and 4.531) mg/L respectively. The GC-MS results indicate the presence of some important long-chain phytoconstituents like linoleic acid, palmitic acid, oleic acid methyl ester, and stearic acid, among others. Furthermore, when testing the possible toxicity of biosynthesized NPs in a non-target organism, no adverse effects were observed in Poecilia reticulata fish exposed for 24 h, considering the evaluated biomarkers. Thus, overall, our study results reveal that biosynthesized TiO2 NPs are an effective and exciting eco-friendly approach to controlling the A. subpictus and C. quinquefasciatus.

In vitro somatic embryo productions from Curculigo orchioides derived gold nanoparticles: Synthesis, characterization, its biomedical applications, and their eco-friendly approaches to degradation of methylene blue under solar light irradiations.

Curculigo orchioides rhizome explants were employed to develop a rapid and effective strategy for increased plant regeneration using somatic embryogenesis. Direct somatic embryo development was shown on rhizome explants cultivated on Murashige and Skoog (MS) making with 2, 4-D (1.0-3.0 mg/L). Rhizome explants cultivated on MS media supplemented with 2.0 mg/L 2, 4-D yielded the highest frequency of embryogenesis (87.5%) and the maximum number of somatic embryos (1596.7/explant). Somatic embryo germination was accomplished using MS media with 2.0 mg/L 6-benzylaminopurine (BAP). With an 80% survival rate, the germination plantlets were acclimated in the greenhouse. The current study is the first evidence of the efficacy of in vitro-produced plants and C. orchioides somatic embryo callus cultures of stable gold nanoparticles. The UV-Vis spectrophotometric absorbance, at 510 nm, revealed the absorption spectra of the AuNPs. The FT-IR revealed functional groups and reaction processes in green AuNP formation. High-resolution transmission electron microscopy (HR-TEM) was used to assess the surface morphology and structure of the AuNPs after their elemental composition was determined using a dispersive energy X-ray (EDAX) spectrum. The average size of AuNPs was around 35 nm in diameter. The crystalline nature of the AuNPs was investigated by X-ray diffraction (XRD). The highest growth inhibition was found for C. orcthioides against Klebsiella pneumoniae (17.5 mm) and Serratia marcescens (16.5 mm). The AuNPs exhibited antioxidant activity against free radicals such as DPPH and ABTS. Furthermore, the cytotoxicity of AuNPs was assessed, and inhibitory concentration (IC50) was 20 µg/mL and 80 µg/mL for breast carcinoma (MDA-MB-231) and Vero cell lines. The degradation of methylene blue measures the photocatalytic activity of the manufactured AuNPs when subjected to visible sunlight (MB). Thus, the result showed a maximum degradation efficiency of MB (84%).

Eco-friendly synthesis of Ag-NPs using Endostemon viscosus (Lamiaceae): Antibacterial, antioxidant, larvicidal, photocatalytic dye degradation activity and toxicity in zebrafish embryos.

Nanotechnology is a multidisciplinary area of study that has grown significantly in serving many functions and impacting human society. New fields of science have been facilitated by the clean, non-toxic, and biocompatible nature of plant-derived nanoparticles. The present study deals with the first green synthesis of silver nanoparticles (Ag-NPs) using Endostemon viscosus, and their synthesized Ag NPs were characterized by different spectral methods (UV-vis Spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), X-ray diffraction Spectroscopy (XRD), Transmission Electron Microscopy (TEM) and Energy dispersive X-ray Spectroscopy (EDAX). The change initially observed the production of Ag-NPs in color from green to ash and then confirmed by SPR band at 435 nm in UV-vis spectral analysis. The FTIR findings indicate that many functional groups belong to the pharmaceutically useful phytochemicals, which interact as reducing, capping, and stabilizing agents in synthesizing silver nanoparticles. The predominant peaks in the XRD pattern belong to the planes 210°, 111°, 200°, 241°, and 311° and thus demonstrated the Ag-NPs FCC crystal structure. TEM analysis exhibited spherical-shaped particles with an average size of 13 nm, and the EDAX band showed a distinctive metallic silver peak at 3.0 keV. The antibacterial activity of Ag-NPs tested to show a maximum zone of inhibition of 19 mm for Staphylococcus aureus and 15 mm for Escherichia coli at 100 µg/mL, respectively. Bio-fabricated Ag-NPs were assessed for antioxidant activity (DPPH with % inhibition 57.54% and FRAP with % inhibition 70.89%). The biosynthesized Ag-NPs demonstrated potential larvicidal efficacy against Aedes aegypti with more than 90% at 250 µg/mL. Histological profiles were altered while treating with Ag-NPs at 250 µg/mL. The photocatalytic activity of synthesized E. viscosus Ag-NPs was tested against methylene blue (MB) and crystal violet (CV), and the maximum degradation efficiency was found as 90 and 94%, respectively. Furthermore, the toxicity test on zebrafish embryos demonstrated that aberrations have only been induced at concentrations higher than 500 µg/mL. We conclude that the greenly produced Ag-NPs may find use in biomedical applications based on bacteria and cost-effective industrial wastewater treatment.

Photobiomodulation Reduces the Cytokine Storm Syndrome Associated with COVID-19 in the Zebrafish Model.

Although the exact mechanism of the pathogenesis of coronavirus SARS-CoV-2 (COVID-19) is not fully understood, oxidative stress and the release of pro-inflammatory cytokines have been highlighted as playing a vital role in the pathogenesis of the disease. In this sense, alternative treatments are needed to reduce the level of inflammation caused by COVID-19. Therefore, this study aimed to investigate the potential effect of red photobiomodulation (PBM) as an attractive therapy to downregulate the cytokine storm caused by COVID-19 in a zebrafish model. RT-qPCR analyses and protein-protein interaction prediction among SARS-CoV-2 and Danio rerio proteins showed that recombinant Spike protein (rSpike) was responsible for generating systemic inflammatory processes with significantly increased levels of pro-inflammatory (il1b, il6, tnfa, and nfkbiab), oxidative stress (romo1) and energy metabolism (slc2a1a and coa1) mRNA markers, with a pattern similar to those observed in COVID-19 cases in humans. On the other hand, PBM treatment was able to decrease the mRNA levels of these pro-inflammatory and oxidative stress markers compared with rSpike in various tissues, promoting an anti-inflammatory response. Conversely, PBM promotes cellular and tissue repair of injured tissues and significantly increases the survival rate of rSpike-inoculated individuals. Additionally, metabolomics analysis showed that the most-impacted metabolic pathways between PBM and the rSpike treated groups were related to steroid metabolism, immune system, and lipid metabolism. Together, our findings suggest that the inflammatory process is an incisive feature of COVID-19 and red PBM can be used as a novel therapeutic agent for COVID-19 by regulating the inflammatory response. Nevertheless, the need for more clinical trials remains, and there is a significant gap to overcome before clinical trials can commence.

Perception and legacy of soil chromium and lead contamination in an operational small-scale coal mining community.

Operational small-scale coal mining (OSCM) is one of the most significant sources of chromium (Cr) and lead (Pb) pollution in Bangladesh. Attempts to minimize or lessen the use of Cr and Pb in OSCM have shown unsatisfactory results, mainly because they need to address the sociotechnical complexity of pollution concerns in OSCM. This research adopts a multidisciplinary, sociotechnical approach to addressing Cr and Pb problems, coupling soil sampling for Cr and Pb with questionnaires of miners' and inhabitants' perceptions of pollution and its distribution. The study was undertaken in the Barapukuria coal basin in northwest Bangladesh. Except for mining areas (average of 49.80 ± 27.25 mg/kg), Cr levels in soils exceeded the world average in the periphery (73.34 ± 24.39 mg/kg, ~ 1.2 times) and residential areas (88.85 ± 35.87 mg/kg, 1.5 times the world standard of 59.5 mg/kg). Pb levels in soils exceeded national and global averages in mining (53.56 ± 37.62 mg/kg, ~ 1.9 times), periphery (35.05 ± 21.77 mg/kg, ~ 1.3 times), and residential areas (32.14 ± 26.59 mg/kg, ~ 1.2 times) when compared to Bangladesh and global standards of 20 and 27 mg/kg. Pb levels were highest in mining areas, while Cr concentrations were highest in residential areas. The questionnaire findings indicated that miners and inhabitants did not correctly assume that the highest levels of Cr and Pb pollution would be found in these areas. Among all respondents, 54% are unaware of the health impacts of prolonged Cr and Pb exposure. They face respiratory problems (38.6%), skin diseases (32.7%), and other health issues. A large number of people (66.6%) agreed with the fact that Cr and Pb contamination has an impact on drinking water. Cr and Pb pollution has caused 40% crop loss and a 36% decrease in productivity in the agricultural sector. However, respondents underestimated the level of Cr pollution in mining areas, and most assumed that only individuals working directly with mines were impacted by the Cr and Pb content. Participants also rated the reduction of Cr and Pb contamination as of low importance. There is less awareness of Cr and Pb pollution among miners and inhabitants. Sincere efforts to reduce Cr and Pb pollution will likely be met with extra attention and hostility.

Toxicity assessment of polyethylene microplastics in combination with a mix of emerging pollutants on Physalaemus cuvieri tadpoles.

Studies in recent years have shown that aquatic pollution by microplastics (MPs) can be considered to pose additional stress to amphibian populations. However, our knowledge of how MPs affect amphibians is very rudimentary, and even more limited is our understanding of their effects in combination with other emerging pollutants. Thus, we aimed to evaluate the possible toxicity of polyethylene MPs (PE-MPs) (alone or in combination with a mix of pollutants) on the health of Physalaemus cuvieri tadpoles. After 30 days of exposure, multiple biomarkers were measured, including morphological, biometric, and developmental indices, behavioral parameters, mutagenicity, cytotoxicity, antioxidant and cholinesterase responses, as well as the uptake and accumulation of PE-MPs in animals. Based on the results, there was no significant change in any of the parameters measured in tadpoles exposed to treatments, but induced stress was observed in tadpoles exposed to PE-MPs combined with the mixture of pollutants, reflecting significant changes in physiological and biochemical responses. Through principal component analysis (PCA) and integrated biomarker response (IBR) assessment, effects induced by pollutants in each test group were distinguished, confirming that the exposure of P. cuvieri tadpoles to the PE-MPs in combination with a mix of emerging pollutants induces an enhanced stress response, although the uptake and accumulation of PE-MPs in these animals was reduced. Thus, our study provides new insight into the danger to amphibians of MPs coexisting with other pollutants in aquatic environments.

Biosynthesis and extrinsic toxicity of copper oxide nanoparticles against cattle parasites: An eco-friendly approach.

Plant extracts' ability to collect metals and decrease metal ions makes them a superior candidate for the biosynthesis of nanoparticles; hence, they are referred to as bio-nano factories since both living and dead dried biomass are employed to produce metallic nanoparticles. The antiparasitic activity of biosynthesized copper oxide nanoparticles (CuO NPs) was examined against cow tick larvae (Rhipicephalus microplus, Haemaphysalis bispinosa, and Hippobosca maculata). These parasitic larvae were treated with various concentrations of methanolic leaf extract of A. marmelos (MLE-AM) and biosynthesized CuO NPs for 24 h. CuO NPs were synthesized quickly using A. marmelos leaf extract, and nanoparticle synthesis was identified within 15 min. The results from characteristic XRD, FTIR, SEM, EDX, and TEM analyses confirmed the biosynthesis of CuO NPs. The presence of 26-Hydroxycholesterol was discovered as the predominant chemical present in the GC-MS analysis of MLE-AM. The maximum efficacy was observed in biosynthesized CuO NPs against R. microplus larvae, H. bispinosa adults, and Hip. maculata larvae (LC50 = 4.30, 9.50, and 11.13 mg/L; and LC90 = 8.30, 19.57, and 21.65 mg/L; and 6.219, 6.547, and 2.587). Overall, the bio-fabrication of CuO NPs has the potential to develop better and safer antiparasitic control techniques.

Green synthesis of gold nanoparticles using Gracilaria crassa leaf extract and their ecotoxicological potential: Issues to be considered.

The use of vegetal species for gold nanoparticles (AuNPs) biosynthesis can constitute an alternative to replacing the extensive use of several hazardous chemicals commonly used during NPs synthesis and, therefore, can reduce biological impacts induced by the release of these products into the natural environment. However, the "green nanoparticles" and/or "eco-friendly nanoparticles" label does not ensure that biosynthesized NPs are harmless to non-target organisms. Thus, we aimed to synthesize AuNPs from seaweed Gracilaria crassa aqueous extract through an eco-friendly, fast, one-pot synthetic route. The formation of spherical, stable, polycrystalline NPs with a diameter of 32.0 nm ± 4.0 nm (mean ±SEM) was demonstrated by UV-vis spectroscopy, field emission scanning electron microscopy, and high-resolution transmission electron microscopy, energy-dispersive X-ray and X-ray diffraction measurement, and Fourier-transform infrared spectroscopy analysis. In addition, different phytocomponents were identified in the biosynthesized AuNPs, using Gas Chromatography-Mass Spectrometry (GC-MS). However, both G. crassa aqueous extract and the biosynthesized AuNPs showed high ecotoxicity in Anopheles stephensi larvae exposed to different concentrations. Therefore, our study supports the potential of seaweed G. crassa as a raw material source for AuNPs biosynthesis while also shedding light on its ecotoxicological potential, which necessitates consideration of its risk to aquatic biota.

Organic waste vermicomposting through the addition of rock dust inoculated with domestic sewage wastewater.

The aims of the present study are to assess the organic waste vermicomposting process (cattle manure mixed with tannery sludge) by using inorganic waste (rock dust) inoculated with treated domestic wastewater sewage, as well as the vermicompost application in Ruellia brittoniana seedling production. Different proportions of organic and inorganic waste moistened (or not) in wastewater were vermicomposted (by Eisenia foetida) for 120 days in the first stage of the experiment. Statistically significant earthworm density increase was observed between the 60th and 90th experimental vermicompositing days in all the assessed groups. There was decreased E. foetida population density after 90 days. The K, P, TOC, C/N ratio and Ca, Na and Mg concentrations significantly decreased at the end of the vermicompositing process in comparison to the initial concentrations identified in most treatments. On the other hand, there was pH and N, Fe, Zn and Mn concentration increase in most of the vermicomposts assessed at the end of the experiment. All plants grown in soil containing vermicomposts presented higher Dickson Quality Index (DQI) than the control group, which was cultivated in soil containing commercial topsoil. Plants grown in soil containing 100% cattle manure and tannery sludge, moistened in treated domestic wastewater sewage, showed the highest DQI. Thus, the vermicomposting waste used in the present study, which was inoculated with treated domestic wastewater sewage, is an interesting vermicompost production technology to be used in ornamental plant production.

Adapting a rapid assessment protocol to environmentally assess palm swamp (Veredas) springs in the Cerrado biome, Brazil.

The exploitation and degradation of natural environments exert intense pressure on important ecosystems worldwide. Thus, it is necessary developing or adapting assessment methods to monitor environmental changes and to generate results to be applied to environmental management programs. The Brazilian Veredas (phytophysiognomies typical to the Cerrado biome) are threatened by several human activities; thus, the aim of the present study is to adapt a rapid assessment protocol (RAP) to be applied to Veredas springs, by using the upper course of the Vai-e-Vem stream watershed (Ipameri County, Goiás State, Brazil). Therefore, several springs in the study site were visited and 11 of them were considered Veredas springs. After the RAP was adapted, the instrument was validated and used to environmentally assess the springs in order to demonstrate its applicability. The present study has provided an instrument of option to monitor Veredas springs.

Zebrafish neuromast sensory system: Is it an emerging target to assess environmental pollution impacts?

Environmental pollution poses risks to ecosystems. Among these risks, one finds neurotoxicity and damage to the lateral line structures of fish, such as the neuromast and its hair cells. Zebrafish (Danio rerio) is recommended as model species to be used in ecotoxicological studies and environmental biomonitoring programs aimed at assessing several biomarkers, such as ototoxicity. However, little is known about the history of and knowledge gaps on zebrafish ototoxicity. Thus, the aim of the current study is to review data available in the scientific literature about using zebrafish as animal model to assess neuromast toxicity. It must be done by analyzing the history and publication category, world production, experimental design, developmental stages, chemical classes, neuromasts and hair cell visualization methods, and zebrafish strains. Based on the results, number, survival and fluorescence intensity of neuromasts, and their hair cells, were the parameters oftentimes used to assess ototoxicity in zebrafish. The wild AB strain was the most used one, and it was followed by Tübingen and transgenic strains with GFP markers. DASPEI was the fluorescent dye most often applied as method to visualize neuromasts, and it was followed by Yo-Pro-1 and GFP transgenic lines. Antibiotics, antitumorals, metals, nanoparticles and plant extracts were the most frequent classes of chemicals used in the analyzed studies. Overall, pollutants can harm zebrafish's mechanosensory system, as well as affect their behavior and survival. Results have shown that zebrafish is a suitable model system to assess ototoxicity induced by environmental pollution.

Microplastic prevalence in epipelagic layer: Evidence from epipelagic inhabiting prawns of north-west Arabian Sea.

The escalating global microplastic (MP) pollution severely threatens marine life due to insufficient waste management and widespread single-use plastic. This study focuses on assessing MP contamination in commercial prawns from Gujarat State, India. Ten prawn species collected at five main fishing harbors revealed 590 MP particles in their gastrointestinal tracts, averaging 6.08 ± 5.96 MPs/g and 1.15 ± 0.78 MPs/individual. Significant variations in contamination levels were observed between species and study sites. Pollution indices indicated very high contamination throughout the study sites. Threads were the predominant shape, with blue and black as prevalent colors. Size-wise, 1-2 mm MPs dominated. Polymer analysis identified polyethylene terephthalate, polyurethane, polystyrene, polypropylene, polyvinyl chloride, and acrylonitrile butadiene styrene. The findings provided crucial preliminary information for ecotoxicology and seafood safety investigations regarding MP contamination in commercially important prawns.

Bo-derived waste neem to enriching reinforced hybrid composite for environmental remediation.

The utilization of natural fibres often entails a lesser environmental impact when compared to synthetic fibres. Biodegradable natural fibres minimize waste and pollution, and promote sustainability, but their weaker bonds limit their resilience. These issues can be addressed by customizing the composite's mechanical properties with natural and synthetic fibres. In this study, hybrid composites were created using the hand layup method with a novel dissimilar layer arrangement of neem (N), sisal (S), and glass (G) fibre and analyze its mechanical and thermal properties. Experimental observation shows that the GN composite had a higher maximum ultimate tensile strength of 26 N/mm2 than the GS, GNS, and GSN composites. The GN composite had a percentage elongation of 6.33%, similar to the percentage elongation of the GS composite (6.833%), and it also had a higher ultimate shear strength of 50 MPa. The composite GS absorbed 6.6 J energy, higher than the composites GN, GNS, and GNS, which absorbed 6.1 J, 4.5 J, and 4.5 J, respectively. The fractured surface's SEM images were obtained and analyzed for failure. The results demonstrated that the hybridization was effective, and better properties can be obtained by combining neem, sisal, and glass fiber, and it can be used for other requirements, including strength, weight, cost, and ecological impact.

Polystyrene nanoplastics induce developmental impairments and vasotoxicity in zebrafish (Danio rerio).

The exponential use of plastics has significantly increased environmental pollution by nanoplastics (NPs). In the aquatic environment, NPs interact and bioaccumulate in the biota, posing a potential ecotoxicological risk. The present study investigated the developmental toxicity, vasotoxicity, cytotoxicity, ROS induction, and behavioral impairments in zebrafish (Danio rerio) exposed to environmentally relevant polystyrene NPs (PS-NPs) concentrations (0.04, 34 ng L-1, and 34 µg L-1) for 144 h through multiple biomarkers response (mortality, frequency of spontaneous contractions, heart rate, and morphological changes). Furthermore, vasotoxicity (head, yolk sac, tail, and branchial vessels) was evaluated using the transgenic zebrafish tg(Fli1:eGFP). Results showed that PS-NPs interacted mainly with zebrafish chorion, gills, tail, and larvae head. PS-NPs at 34 ng L-1 and 34 µg L-1 induced neurotoxicity (decreased frequency of spontaneous contractions), cardiotoxicity (bradycardia), and morphological changes in the eyes and head, indicating that PS-NPs induce developmental impairments in zebrafish. In addition, cytotoxicity in the caudal region (34 ng L-1), ROS production, decreased mean swimming speed, and distance covered were observed in all tested concentrations. PS-NPs also induced vasotoxicity (yolk sac region) in transgenic zebrafish. Overall, the present study demonstrates the harmful effects of PS-NPs on the early developmental stages of freshwater fish, indicating their environmental risk.