New technique of intragastric sleeve: viability and survival in a pig model.

Developing a less invasive, practical and cost-effective operative technique for obesity treatment represents a pressing need for our society. In this way, intragastric single port sleeve by endoplication was tested in six pigs during 18 weeks. Celiotomy was performed with animal placed in dorsal decubitus position. Single port gastrostomy was performed and double tobacco pouch sutures were made in fundic region, making a gastric sleeve. At the end, stomach layers and skin were closed in a conventional manner. Means and the standard deviations of surgical time were calculated. The procedure was simple and all animals survived; there were no significant blood loss and no intra and postoperative complications. The procedure was fast (67.4 minutes). The technique has the advantage of not requiring the use of mechanical sutures, making it less costly. The innovation of this procedure was the use of a single port gastrostomy device to perform an intraluminal sleeve. What made this technique less invasive were the use of a single port, nonmanipulation of the stomach intra-abdominally, ease of execution and no need of pneumoperitoneum. The new technique is acceptable and has reproducible viability, had a short procedure time without intra and postoperative complications.

Neonatal Candidemia Caused by Candida haemulonii: Case Report and Review of Literature.

Candidemia is a frequent condition in Neonatal Intensive Care Units (NICU) and usually complicates the newborns clinical course. Several factors are responsible for candidiasis, such as prematurity and use of broad-spectrum antibiotics, and in these cases, there are the involvement of various Candida species, as C. albicans, and C. parapsilosis. However, other species as C. haemulonii has been rarely described in candidemia cases, being considered an emergent pathogen. Thus, we report a case of neonatal candidemia by C. haemulonii and a review of literature of fungemia by this yeast. The patient was a neonate with gestational age of 26 weeks and birth weight of 660 g hospitalized in a NICU from a Brazilian hospital. The identification of the etiological agent was performed by phenotypic methods, scanning electron microscopy, sequencing of the ITS region of rDNA, and mass spectrometry. Antifungal susceptibility testing was carried out according to the Clinical Laboratories and Standards Institute guidelines. The newborn was diagnosed with candidemia by C. haemulonii resistant to amphotericin B with minimal inhibitory concentration (MIC) of 8 µg/mL, sensitive to fluconazole (MIC: 8 µg/mL) and voriconazole (MIC: 0.12 µg/mL). The treatment with fluconazole (12 mg/kg/day) was established with good outcome. Candidemia by C. haemulonii is still being limited to a few sporadic cases in adults with endemic and restricted occurrences in neonates. Usually, the therapy with amphotericin B is ineffective against this species. Our results showed the importance of the mycological diagnosis associated to antifungigrama for the successful clinical management followed by important epidemiological data.

Lethal destructive sinusopathy due to amphotericin B-resistant Aspergillus flavus: A case report.

BACKGROUND: The prevalence of pulmonary aspergillosis and the importance of its early diagnosis are recognized. However, non-pulmonary involvement, including the sinuses region, is not frequently reported, and an infection in this area can affect all paranasal sinuses (pansinusopathy), being a rare pathology that affects immunocompromised hosts. Recent studies have highlighted the occurrence of Aspergillus flavus resistant to antifungal therapy. Therefore, a nasal sinus infection by resistant Aspergillus strains in immunocompromised patients may be linked to a high risk of lethality. CASE REPORT: We are reporting a resistant A. flavus infection in an allogeneic hematopoietic stem cell transplant recipient with episodes of febrile neutropenia, and prolonged use of various antibacterial drugs and antifungal prophylaxis. The patient underwent brain magnetic resonance, which showed the presence of pansinusopathy, and presented necrosis in the left nasal region. Direct microscopic examination of a sample taken from the nasal mucosa revealed the presence of septate hyphae and conidiophores resembling those of A. flavus, that species being the identification achieved with MALDI-TOF MS. Antifungigram was performed by microdilution in broth (EUCAST-E.DEF. 9.3.2) and E-test, and resistance to amphotericin B was shown in both tests. The patient died after septic shock and hemorrhage. CONCLUSIONS: Invasive fungal infections due to amphotericin-B resistant A. flavus may lead to the death of the patient due to an ineffective therapeutic management. Therefore, antifungal susceptibility testing are of utmost importance for administering the proper treatment.

Predictive Score for Carbapenem-Resistant Gram-Negative Bacilli Sepsis: Single-Center Prospective Cohort Study.

A clinical-epidemiological score to predict CR-GNB sepsis to guide empirical antimicrobial therapy (EAT), using local data, persists as an unmet need. On the basis of a case-case-control design in a prospective cohort study, the predictive factors for CR-GNB sepsis were previously determined as prior infection, use of mechanical ventilation and carbapenem, and length of hospital stay. In this study, each factor was scored according to the logistic regression coefficients, and the ROC curve analysis determined its accuracy in predicting CR-GNB sepsis in the entire cohort. Among the total of 629 admissions followed by 7797 patient-days, 329 single or recurrent episodes of SIRS/sepsis were enrolled, from August 2015 to March 2017. At least one species of CR-GNB was identified as the etiology in 108 (33%) episodes, and 221 were classified as the control group. The cutoff point of ≥3 (maximum of 4) had the best sensitivity/specificity, while ≤1 showed excellent sensitivity to exclude CR-GNB sepsis. The area under the curve was 0.80 (95% CI: 0.76-0.85) and the number needed to treat was 2.0. The score may improve CR-GNB coverage and spare polymyxins with 22% (95% CI: 17-28%) adequacy rate change. The score has a good ability to predict CR-GNB sepsis and to guide EAT in the future.

Mast cell repopulation of the peritoneal cavity: contribution of mast cell progenitors versus bone marrow derived committed mast cell precursors.

BACKGROUND: Mast cells have recently gained new importance as immunoregulatory cells that are involved in numerous pathological processes. One result of these processes is an increase in mast cell numbers at peripheral sites. This study was undertaken to determine the mast cell response in the peritoneal cavity and bone marrow during repopulation of the peritoneal cavity in rats. RESULTS: Two mast cell specific antibodies, mAb AA4 and mAb BGD6, were used to distinguish the committed mast cell precursor from more mature mast cells. The peritoneal cavity was depleted of mast cells using distilled water. Twelve hours after distilled water injection, very immature mast cells could be isolated from the blood and by 48 hours were present in the peritoneal cavity. At this same time the percentage of mast cells in mitosis increased fourfold. Mast cell depletion of the peritoneal cavity also reduced the total number of mast cells in the bone marrow, but increased the number of mast cell committed precursors. CONCLUSIONS: In response to mast cell depletion of the peritoneal cavity, a mast cell progenitor is released into the circulation and participates in repopulation of the peritoneal cavity, while the committed mast cell precursor is retained in the bone marrow.

Effects of Flunixin Meglumine, Firocoxib, and Meloxicam in Equines After Castration.

This study is "aimed" to evaluate and compare the efficacy of flunixin meglumine (FM), firocoxib (FX), and meloxicam (MX) after castration of horses. Thirty horses were submitted to open castration and divided into three groups (n = 10) depending on the anti-inflammatory drug administered: group I (GI) (FM, 1.1 mg kg1, IV, once a day [SID], 5 days); group II (GII) (FX, 0.1 mg kg1, IV, SID, 5 days), and group III (GIII) (MX, 0.6 mg kg1, IV, SID, 5 days). Clinical, behavioral, and hematological parameters and the peritoneal fluid (PF) were evaluated before (day [D] 0) and 1, 2, 3, 5, and 7 days afterward. In the postoperative, scores of limb rigidity and prepuce edema of animals of GII and GIII were greater than those of GI. Tachycardia was observed in the horses of GII and GIII and hyperthermia in GIII. An increase in the number of leukocytes, neutrophils, and monocytes without exceeding the reference values and hyperfibrinogenemia was observed in the animals of GI (D7), GII (D1-D7), and GIII (D7). There was reduction in serum protein after castration, together with an increase of this in the PF of the animals of the three groups. The PF on D0 was straw yellow and limpid, became reddish and cloudy on D1, and then gradually moved toward its normal color on the ensuing days, but without returning to normal on D7 in any of the groups. The results showed that castration triggers significant clinical and laboratory changes and that FM, FX, and MX are equally effective in controlling pain and inflammation in horses after castration; however, FM was more advantageous.

Long-Term Outcomes among Kidney Transplant Recipients and after Graft Failure: A Single-Center Cohort Study in Brazil.

BACKGROUND: The results of kidney transplantation are impacted by the categories of events responsible for patient death and graft failure. The objective of this study was to evaluate the causes of death and graft failure and outcomes after graft failure among kidney transplant recipients. METHODOLOGY: A retrospective cohort study was conducted with 944 patients who underwent kidney transplantation. Outcomes were categorized in a managed and hierarchical manner. RESULTS: The crude mortality rate was 10.8% (n=102): in 35.3% cause of death was infection, in 30.4% cardiovascular disease, and in 15.7% neoplasia and in 6.8%, it was not possible to determine the cause of death. The rate of graft loss was 10.6%. The main causes of graft failure were chronic rejection (40%), acute rejection (18.3%), thrombosis (17.3%), and recurrence of primary disease (16.5%). Failures due to an acute rejection occurred earlier than those due to chronic rejection and recurrence (p<0.0001). As late causes of graft loss, death with the functioning kidney occurred earlier than recurrence and chronic rejection (p=0.008). The outcomes after graft failure were retransplantation in 26.1% and death in 21.4%, at a mean of 25.5 and 21.4 months, respectively. CONCLUSION: It was possible to identify more than 90% of the events responsible for the deaths of transplanted patients, predominantly infectious and cardiovascular diseases. Among the causes of graft failure, chronic and acute rejections and recurrence were the main causes of graft failure which were followed more frequently by retransplantation than by death on dialysis.

ELISA of amyloid A in paired bronchoalveolar lavage fluid and serum samples of healthy horses / Detecção de amilóide A no lavado broncoalveolar de equinos hígidos

Pulmonary disorders are common in horses, and treatment efficiency depends on an adequate diagnosis. Amyloid A is the most sensitive indicator of pathology in horses. The objective of this study was to establish the concentration of amyloid A of bronchoalveolar lavage fluid (BALF) in healthy horses. Health condition of horses was considered normal based on physical examination, complete blood count, biochemical parameters, and BALF cytology. Blood and BALF were collected from thirty adult female horses. Amyloid A concentrations in serum and BALF were measured using commercial ELISA tests. Amyloid A was detected in serum (mean ± SD = 3.71±2.51) and BALF (mean ± SD = 0.000745±0.000785) of all horses. In conclusion, SAA can also be measured in bronchoalveolar fluid, affording early detection of respiratory infections or inflammatory conditions.(AU)

Distúrbios pulmonares são comuns nos cavalos e a eficiência do tratamento depende de um diagnóstico adequado e precoce. A amilóide A é um biomarcador sensível na deteccção de patologias inflamatórias e infecciososa em cavalos. O objetivo deste estudo foi estabelecer a concentração de amilóide A no líquido broncoalveolar (LBA) em cavalos saudáveis. Os cavalos foram considerados saudaveis baseado nos achados de normalidade do exame físico, hemograma, parâmetros bioquímicos e citologia do LBA. Sangue e LBA foram coletados de 30 fêmeas equinas adultas. Os níveis de Amilóide A no soro e no LBA foram mensurados por meio do teste de ELISA. A amilóide A foi detectada no soro (média ± DP = 3,71±2,51) e no LBA (média ± DP = 0,000745±0,000785) de todos os animais. Conclui-se que a amilóide A também pode ser mensurada no LBA, auxiliando no diagnóstico precoce de processos inflamatórios e infecciosos pulmonares.(AU)

Association between obesity and knee osteoarthritis in users of brazilian unified national health system / Associação entre obesidade e osteoartrite de joelhos em usuários do Sistema Único de Saúde

Verify possible associations between obesity and knee OA in users of Brazilian Unified National Health System (Sistema Único de Saúde - SUS) of primary care network in Catalão-GO, Brazil. This is an observational study conducted in 2014 and 2015. A total of 81 volunteers from the Basic Attention Network in the city of Catalão, with body mass index (BMI) over 30 kg/m2 in both sexes, age between 40 and 60 years, and OA on radiological examination of the knee. Obesity was classified according to BMI. The diagnosis of osteoarthritis (OA) was made clinically and by radiography according to Kellgren radiological classification. The level of physical activity was assessed using the Baecke questionnaire and the Gordon Functional Classification. The volunteers underwent an anthropometric evaluation and physical examination with inspection and palpation of the joint and subsequent radiological examination of the knee. The volunteers answered a questionnaire on the level of physical activity and functional classification. Data analysis was performed using Fisher's exact test or chi-square test was used for comparisons of two proportions. In comparisons of continuous variables, Student's t-test or the nonparametric Wilcoxon-Mann-Whitney test was used. The level of significance was set to 5%. Practice of regular physical exercise decreases functional impairment in obese individuals with knee OA. The weight variable demonstrated a strong association with the severity of OA and degree of functionality of patients. Obesity and OA durations and lack of patient guidance are variables that may contribute to the progression of knee OA. Although the incidence among men is lower, they are more severely affected and anthropometric evaluation and physical examination are an efficacy implementations for Brazilian users of SUS.

Verificar possíveis associações entre obesidade e osteoartrite (OA) de joelho em usuários do Sistema Único de Saúde (SUS) da cidade de Catalão-GO. Trata-se de um estudo observacional realizado entre 2014 e 2015. Participaram deste estudo 81 voluntários da Rede de Atenção Básica da cidade de Catalão, com índice de massa corporal (IMC) acima de 30 kg / m2 em ambos os sexos, idade entre 40 e 60 anos e OA no exame radiológico do joelho. A obesidade foi classificada de acordo com o IMC. O diagnóstico de OA foi feito clinicamente e por radiografia de acordo com a classificação radiológica de Kellgren. O nível de atividade física foi avaliado pelo questionário de Baecke e pela Classificação Funcional de Gordon. Os voluntários responderam a uma avaliação antropométrica e exame físico com inspeção e palpação da articulação e posterior exame radiológico do joelho. Os voluntários responderam a um questionário sobre o nível de atividade física e classificação funcional. Os dados foram analisados por meio do teste exato de Fisher ou o teste do qui-quadrado foi utilizado para comparações de duas proporções. Nas comparações das variáveis contínuas, utilizou-se o teste t de Student ou o teste não paramétrico de Wilcoxon-Mann-Whitney. O nível de significância foi estabelecido em 5%. A prática de exercício físico regular diminui o comprometimento funcional em indivíduos obesos com OA de joelho. A variável peso demonstrou uma forte associação com a gravidade da OA e o grau de funcionalidade dos pacientes. Obesidade e duração da OA e falta de orientação do paciente são variáveis que podem contribuir para a progressão da OA do joelho. Embora a incidência entre os homens seja menor, eles são mais gravemente afetados. Adicionalmente, a avaliação antropométrica e o exame físico são procedimentos complementares eficazes para usuários brasileiros do SUS.

Infection by multidrug-resistant Elizabethkingia meningoseptica: case reports / Infecção por Elizabethkingia meningoseptica multirresistente: relato de casos

We report two cases of sepsis in critically ill patients in two tertiary care hospitals in Recife-PE, Brazil. The first case is an 87-year-old patient with chronic myeloid leukemia and sepsis; and the second case is a 93-year-old patient with prostate cancer and septic shock caused by multidrug-resistant (MDR) Elizabethkingia meningoseptica.

Reportamos dois casos de sepse em pacientes criticamente debilitados em dois hospitais com nível de complexidade terciária em Recife-PE, Brasil. O primeiro caso, paciente de 87 anos com leucemia mieloide crônica e sepse; o segundo, paciente com 93 anos de idade com câncer de próstata apresentava choque séptico causado por Elizabethkingia meningoseptica multirresistente.

Conhecimento dos secretários de saúde dos municípios da III regional sobre a politica nacional de triagem neonatal / Knowledge of the health secretaries of the III regional municipalities on national the policy for newborn screening

Objetivo: Avaliar o conhecimento dos Secretários de Saúde dos municípios da III regional sobre a Política Nacional de Triagem Neonatal. Método: Realizou-se um estudo do tipo descritivo e quantitativo. Foram analisadas: se os secretários de saúde dos municípios da III regional conhecem a Política Nacional de Triagem Neonatal; se o município executa esta política; as necessidades de orientações dos secretários de saúde sobre esta política. Os municípios foram caracterizados pelo Índice de Desenvolvimento Humano. Resultados: Identificou-se que menos de 50 por cento dos gestores locais conhecem a PNTN, associada a análise do tempo de gestão que evidenciou não interferir diretamente no conhecimento da PNTN, como também apenas 60 por cento dos que afirmam conhecer a PNTN têm postos de coleta para o Teste do Pezinho. Apenas 23 por cento dos secretários referem ter sido procurados pela Secretaria de saúde Estadual para tratar sobre a PNTN. Conclusão: O desconhecimento dos gestores sobre a PNTN, priva a população do direito à saúde universal e integral, provavelmente por interferência de fatores organizacionais, exigindo medidas corretivas das três esferas que sejam condizentes com o Programa Nacional de Triagem Neonatal

Conhecimento dos secretários de saúde dos municípios da III regional sobre a politica nacional de triagem neonatal / Knowledge of the health secretaries of the III regional municipalities on national the policy for newborn screening

Objetivo: Avaliar o conhecimento dos Secretários de Saúde dos municípios da III regional sobre a Política Nacional de Triagem Neonatal. Método: Realizou-se um estudo do tipo descritivo e quantitativo. Foram analisadas: se os secretários de saúde dos municípios da III regional conhecem a Política Nacional de Triagem Neonatal; se o município executa esta política; as necessidades de orientações dos secretários de saúde sobre esta política. Os municípios foram caracterizados pelo Índice de Desenvolvimento Humano. Resultados: Identificou-se que menos de 50 por cento dos gestores locais conhecem a PNTN, associada a análise do tempo de gestão que evidenciou não interferir diretamente no conhecimento da PNTN, como também apenas 60 por cento dos que afirmam conhecer a PNTN têm postos de coleta para o Teste do Pezinho. Apenas 23 por cento dos secretários referem ter sido procurados pela Secretaria de saúde Estadual para tratar sobre a PNTN. Conclusão: O desconhecimento dos gestores sobre a PNTN, priva a população do direito à saúde universal e integral, provavelmente por interferência de fatores organizacionais, exigindo medidas corretivas das três esferas que sejam condizentes com o Programa Nacional de Triagem Neonatal (AU)

Conhecimento dos secretários de saúde dos municípios da III regional sobre a politica nacional de triagem neonatal / Knowledge of the health secretaries of the III regional municipalities on national the policy for newborn screening

Objetivo: Avaliar o conhecimento dos Secretários de Saúde dos municípios da III regional sobre a Política Nacional de Triagem Neonatal. Método: Realizou-se um estudo do tipo descritivo e quantitativo. Foram analisadas: se os secretários de saúde dos municípios da III regional conhecem a Política Nacional de Triagem Neonatal; se o município executa esta política; as necessidades de orientações dos secretários de saúde sobre esta política. Os municípios foram caracterizados pelo Índice de Desenvolvimento Humano. Resultados: Identificou-se que menos de 50 por cento dos gestores locais conhecem a PNTN, associada a análise do tempo de gestão que evidenciou não interferir diretamente no conhecimento da PNTN, como também apenas 60 por cento dos que afirmam conhecer a PNTN têm postos de coleta para o Teste do Pezinho. Apenas 23 por cento dos secretários referem ter sido procurados pela Secretaria de saúde Estadual para tratar sobre a PNTN. Conclusão: O desconhecimento dos gestores sobre a PNTN, priva a população do direito à saúde universal e integral, provavelmente por interferência de fatores organizacionais, exigindo medidas corretivas das três esferas que sejam condizentes com o Programa Nacional de Triagem Neonatal.