Mitochondrial responses towards intermittent heat shocks in the eastern oyster, Crassostrea virginica.

Frequent heat waves caused by climate change can give rise to physiological stress in many animals, particularly in sessile ectotherms such as bivalves. Most studies characterizing thermal stress in bivalves focus on evaluating the responses to a single stress event. This does not accurately reflect the reality faced by bivalves, which are often subject to intermittent heat waves. Here, we investigated the effect of intermittent heat stress on mitochondrial functions of the eastern oyster, Crassostrea virginica, which play a key role in setting the thermal tolerance of ectotherms. Specifically, we measured changes in mitochondrial oxygen consumption and H2O2 emission rates before, during and after intermittent 7.5°C heat shocks in oysters acclimated to 15 and 22.5°C. Our results showed that oxygen consumption was impaired following the first heat shock at both acclimation temperatures. After the second heat shock, results for oysters acclimated to 15°C indicated a return to normal. However, oysters acclimated to 22.5°C struggled more with the compounding effects of intermittent heat shocks as denoted by an increased contribution of FAD-linked substrates to mitochondrial respiration as well as high levels of H2O2 emission rates. However, both acclimated populations showed signs of potential recovery 10 days after the second heat shock, reflecting a surprising resilience to heat waves by C. virginica. Thus, this study highlights the important role of acclimation in the oyster's capacity to weather intermittent heat shock.

Siltation negatively affects settlement and gaping behaviour in eastern oysters.

While high levels of siltation are known to be deleterious to eastern oysters (Crassostrea virginica), the collective effect of suspended and bedded sediment is understudied from the perspective of oyster farming and bed restoration. In this study, we used laboratory experiments to explore spat settlement rates on a wild bed proxy substrate (i.e., empty shells on the bottom of experimental tanks) in conditions simulating a siltation event and the presence of suspended spat collectors. Using high-frequency valvometry, we also described the behavioural effects of acute sediment burial on wild adult oysters in situ. The vast majority of larvae settled on bottom substrate as opposed to suspended collectors. Sediment negatively affected overall oyster spat settlement on bottom shell, as spat densities were ≈3 × lower when sediment was present. This negative effect was largely attributed to severely depressed spat densities on the upper side (top) of bottom shells. Settlement on the underside of bottom shell was less affected. Wild adult oyster behaviour was negatively affected by acute burial, which ultimately resulted in death. We suggest that the reduction in settlement in the presence of siltation is likely due to the combined effects of suspended sediment on cue detection and bedded sediment on substrate availability. Given that oysters are ecosystem engineers, the negative effects of siltation on both larval and adult oysters can ultimately result in cascading effects to the surrounding biological community.