Fatty-acid profiles of white muscle and liver in stream-maturing steelhead trout Oncorhynchus mykiss from early migration to kelt emigration.

The profiles of specific fatty acids (FA) in white muscle and liver of fasting steelhead trout Oncorhynchus mykiss were evaluated at three periods during their prespawning migration and at kelt emigration in the Snake-Columbia River of Washington, Oregon and Idaho, to improve the understanding of energy change. Twenty-seven FAs were identified; depletion of 10 of these was positively correlated in liver and white muscle of prespawning O. mykiss. To observe relative changes in FA content more accurately over sampling intervals, the lipid fraction of tissues was used to normalize the quantity of individual FA to an equivalent tissue wet mass. Saturated and monounsaturated FAs were depleted between upstream migration in September and kelt emigration in June, whereas polyunsaturated FAs were more conserved. Liver was depleted of FAs more rapidly than muscle. Three FAs were detected across all sampling intervals: 16:0, 18:1 and 22:6n3, which are probably structurally important to membranes. When structurally important FAs of O. mykiss are depleted to provide energy, physiological performance and survival may be affected.

The Limulus amebocyte lysate assay. A rapid and sensitive method for diagnosing early gram-negative peritonitis in patients undergoing continuous ambulatory peritoneal dialysis.

The Limulus amebocyte lysate (LAL) assay was used in a blinded, prospective fashion to analyze peritoneal fluids from 35 consecutive patients undergoing continuous ambulatory peritoneal dialysis (CAPD), who presented with clinical peritonitis. The results were correlated with standard microbiologic culture results. The LAL assay was positive in all three patients with gram-negative peritonitis, was appropriately negative in 24 of 28 gram-positive infections (sensitivity, 100%; specificity, 86%) and was positive in two of five cases in which there was no microbiologic growth. One of the two patients in this last group yielded a gram-negative organism two days later. It was then demonstrated that therapeutic concentrations of a variety of antibiotics (cefazolin sodium, gentamicin sulfate, tobramycin sulfate, ticarcillin disodium, penicillin G potassium, vancomycin hydrochloride, metronidazole hydrochloride, piperacillin sodium, and trimethoprim/sulfamethoxazole) did not interfere with the LAL assay. Together, these data indicate that the LAL assay is useful for identifying patients at high risk for gram-negative peritonitis and for excluding from possible aminoglycoside exposure the majority of patients with peritonitis undergoing CAPD, most of whom will have gram-positive infections. Furthermore, lack of antibiotic interference allows the possibility of monitoring treatment efficacy.

Equilibrium vapour pressure of mercury from dental amalgam under loading conditions.

Earlier studies have failed to establish a consensus on the amount of mercury vapour released from dental amalgam restorations. The purpose of this study was to accurately and quantitatively measure the equilibrium mercury vapour pressure from dental amalgam. The vapour pressure was measured using a quartz crystal microbalance as a function of the load from 0-5.4 MPa. Auger spectra were collected of the as-formed and argon ion sputter cleaned dental amalgam surface. For the as-formed surface the mercury vapour density is zero with no load and increases to 0.6 microg m(-3) at 5.4 M Pa. Following cleaning the mercury, vapour density increased to a maximum value of 15 microg m(-3). The Auger spectra of the as-formed surfaces were dominated by features associated with carbon and oxygen. These spectral results in concert with the mercury vapour density measurements indicate that the oxide film on the as-formed surfaces inhibited the release of mercury vapour. The results of this experiment provide an upper limit for the amount of mercury vapour released by dental amalgams. Under conditions simulating the oral cavity this value would be reduced by oxides that form on the surface of dental amalgam restorations.

Assessment of symptoms of DSM-IV anxiety and depression in children: a revised child anxiety and depression scale.

The practical significance of assessing disorders of emotion in children is well documented, yet few scales exist that possess conceptual if not empirical relevance to dimensions of DSM anxiety or depressive disorders. The current study evaluated an adaptation of a recently developed anxiety measure (Spence Children's Anxiety Scale; [Spence, S. H. (1997). Structure of anxiety symptoms among children: a confirmatory factor-analytic study. Journal of Abnormal Psychology, 106, 280-297; Spence, S. H. (1998). A measure of anxiety symptoms among children. Behaviour Research and Therapy, 36, 545-566]), revised to correspond to dimensions of several DSM-IV anxiety disorders as well as major depression. This investigation involved initial evaluation of the factorial validity of the revised measure in a school sample of 1641 children and adolescents and reliability and validity in an independent sample of 246 children and adolescents. Results yielded an item set and factor definitions that demonstrated structure consistent with DSM-IV anxiety disorders and depression. The revised factor structure and definitions were further supported by the reliability and validity analyses. Some implications for assessment of childhood anxiety and depressive disorders are discussed.

Relations of tripartite dimensions of emotion to childhood anxiety and mood disorders.

This study examined preliminary higher-order models relating tripartite dimensions of emotion to severity of anxiety and depressive disorders in 100 clinically referred children and adolescents. In light of the accumulating support for multifactor models of vulnerability and negative emotion in children, the present investigation was designed to establish preliminary estimates of the structure and magnitude of the relations of three emotion factors with dimensions of social anxiety, depression, panic, generalized anxiety, obsessions/compulsions, and separation anxiety. Results were consistent with structures that minimally specified two higher-order emotion factors, yet only some parameter estimates were consistent with theory regarding the tripartite model. Problems with the measurement of tripartite factors and possibilities for further research are outlined.

Equilibrium vapor pressure of mercury from dental amalgam in vitro.

OBJECTIVES: The purpose of this study was to acquire reliable quantitative data on mercury vapor pressure above the surface of dental amalgam samples in both the as-formed and the abraded state. METHODS: The equilibrium vapor pressure of mercury from dental amalgam was obtained using residual gas analysis in conjunction with a quartz crystal microbalance. The measurements were performed under ultrahigh-vacuum conditions. RESULTS: Using the residual gas analyzer, the average mercury vapor pressures measured from the as-formed and abraded dental amalgam surfaces were 8 x 10(-7) and 1.7 x 10(-4) Pa, respectively. The corresponding vapor densities are: 6 x 10(-2) micrograms/m3 and 14 micrograms/m3. The average mercury vapor pressures measured from the as-formed and abraded dental amalgam surfaces using the quartz crystal microbalance were: 2.4 x 10(-6) and 1.0 x 10(-4) Pa, respectively. The corresponding vapor densities are: 0.2 microgram/m3 and 8 micrograms/m3. SIGNIFICANCE: This project provides a measurement of the equilibrium vapor pressure of mercury from as-formed and abraded amalgam surfaces. By definition, the equilibrium vapor pressure provides the maximum density of mercury which can be present in the environment above the amalgam. The vapor density of mercury within the oral cavity must be less than this value due to the dynamic nature of the system.

Oral and injectable applications of erythromycin in salmonid fish culture.

Erythromycin is a macrolide antibiotic effective against Renibacterium salmoninarum, causative agent of bacterial kidney disease in salmonids. Although erythromycin is used on an experimental basis in private and conservation hatcheries, the drug is not registered with the US Food and Drug Administration for use in fish culture. Residues of erythromycin are retained in the tissues of juvenile and adult salmon for some time following administration of oral or injectable forms of the drug, a characteristic that may be important in the drug's efficacy against the slow growing R. salmoninarum pathogen. Before erythromycin can be registered, additional information must be collected about efficacy, toxicity, and environmental consequences of administration.

Field trials of investigational new animal drugs.

Field trials provide an opportunity to determine the efficacy and safety of drug treatments in a variety of environments and fish stocks that would not be possible or practical in laboratory settings. The steps for executing good field trials are discussed. The University of Idaho coordinates numerous field trials at over 100 hatcheries throughout the Pacific northwest as part of an intensive and extensive program to register erythromycin injectable and feed additive to control bacterial kidney disease in salmonids. A standardized toxicity test conducted at 52 hatcheries following the completion of administration of erythromycin feed additive provided a way to compare responses by fish at different times of the year, locations, and among different sizes of fish. Some of these fish were used in an assay to quantify the content of erythromycin in kidney tissue post treatment.

Gonococcal peritonitis in a patient treated with continuous ambulatory peritoneal dialysis (CAPD).

We report a case of gonococcal peritonitis in a sexually active female on continuous ambulatory peritoneal dialysis. The presumed route of entry into the peritoneal cavity was via the Fallopian tube. The episode responded rapidly to a standard antibiotic treatment protocol. Some episodes of culture-negative peritonitis may be caused by genital tract organisms which are not cultured by routine methods.