Axial load weightbearing radiography in determining lateral malleolus fracture stability: a cadaveric study.

BACKGROUND: Ankle fractures are the most common fracture treated by orthopaedic surgeons in the United States. Isolated lateral malleolus fractures are treated nonoperatively, while associated deltoid disruption injuries are unstable and usually treated by fixation of the fracture. Various stress radiographs and MRI have been used to determine deltoid competency and the subsequent need for operative stabilization of lateral malleolus fractures. To date, no standardization of stress radiography has been performed. The aim of this study was to evaluate the ability of weightbearing ankle X-rays to determine stability and their reliability. METHODS: This study sought to utilize simulated weightbearing radiography as a potential method to determine the need for lateral malleolus fixation. Twelve cadaveric ankle specimens were tested by obtaining ankle mortise radiographs for interpretation of medial clear space. Each ankle was tested with 0, 25, 36, and 50 kg of axially applied weight. The groups of measurements obtained were: intact ankles (Group A), ankles with an isolated oblique fibular osteotomy (Group B), and osteotomized ankles after complete deltoid ligament transaction (Group C). Three authors measured the medial clear space for every ankle using PACSR software. Instability of the ankle mortise was defined as medial clear space widening of greater than 2 mm from the intact ankle. RESULTS: An isolated lateral malleolus fracture (Group B) and a lateral malleolus fracture with an incompetent deltoid ligament (Group C) showed no statistical medial clear space widening with simulated axial weightbearing radiography when compared to intact ankles (Group A). ANOVA's between Group A and B had p < 0.001, and between Group A and C of p < 0.001. CONCLUSION: Our model did not find instability with an osteotomized fibula and a disrupted deltoid. CLINICAL RELEVANCE: This study suggests a weightbearing radiograph of an isolated lateral malleolus fracture cannot determine deltoid ligament integrity and thus need for fibular operative fixation.

Bioabsorbable fixation: scientific, technical, and clinical concepts.

Bioabsorbable internal fixation has been clinically available for nearly 20 years and has been used for hard tissue and/or soft tissue fixation from the head to toe. During this time, there has been a rapid evolution in bioabsorbable polymers as well as in the implants and their clinical applications. There is a plethora of bioabsorbable polymers now available for clinical use, and although all are related chemically, seemingly small changes in formulation can have a dramatic effect on their biomechanical and physiological properties. This article summarizes much of the relevant scientific, technical, and clinical literature in a simple, straightforward manner to help surgeons better understand and appreciate the nuances of bioabsorbable fixation. This up-to-date review should also help researchers establish a baseline of knowledge that can provide a platform for further developments in this exciting field.

A quantitative analysis of tension band plating of the femur diaphysis.

OBJECTIVES: To measure quantitatively the strains in the concave and convex sides of a femur to substantiate the Arbeitsgemeinschaft für Osteosynthesefragen (AO) tension band principle. METHODS: Eight cadaveric fresh-frozen femurs were cleaned of soft tissue and prepared for mounting the strain gages (Vishay, Raleigh, NC). The student strain gages were mounted at the anterior, lateral and medial surfaces of a femur. They were loaded at 100, 250, 500, 1,000 and 1,500 N axial forces in a mechanical testing machine (Instron Model 4202, Norwood, MA) and the micro strains were measured for intact and after fixation of a simulated fracture by an eight hole broad 4.5 mm stainless steel compression plate (Synthes LCP). In order to simulate the fracture conditions, where all eight screw holes might not be filled, three different arrangements were tested: 8 screws, 4 screws and 2 screws. The microstrains under different loads were analyzed by Analysis of Variance (ANOVA) with Holm-Sidak multiple comparison method. RESULTS: At the same gage location micro strains were not significantly different between the intact, and the femur fixated by 8, 4 and 2 screws. For intact bones at 1,500 N the strain ratio between the tension to compression sides was -0.21 proximally and -0.04 distally. The comparison of these strain ratios at different loads did not show any significant differences at p = 0.05 and power of 0.8. CONCLUSIONS: The data showed a trend validating the tension band principle as tensile strains lowered and compressive strains increased after placement of the plate.

Changes in load distribution in the knee after opening-wedge or closing-wedge high tibial osteotomy.

The treatment of unicompartmental osteoarthritis of the knee by high tibial osteotomy has been carried out by closing-wedge osteotomy. The advantages for opening-wedge osteotomy are ease of procedure and improved correction with comparable short-term to midterm results. It is not known how the opening-wedge high tibial osteotomy procedure alters the load distribution between the medial and lateral compartments of the knee. The current biomechanical study investigated opening-wedge vs closing-wedge osteotomies in 5 pairs of cadaver knees. The results showed that at 5 degrees osteotomy, the closing-wedge provided superior results of load transfer from medial to lateral compartment than that seen with opening-wedge, but at 10 degrees osteotomy, there was no significant difference in load transfer in the knee compartments between the 2 surgery modes.

Comparison of pressure effects on adjacent disk levels after 2-level lumbar constructs: fusion, hybrid, and total disk replacement.

BACKGROUND: With increasing advocacy for the use of TDR procedure as a surgical alternative to fusion in the management of lumbar DDD, intradiskal pressures at the adjacent levels of spine have generated considerable interest. The common belief is that adjacent-level disk pressures will be lower after a TDR as opposed to conventional fusion. The aim of this study is to present the effect of different constructs on adjacent-level disk pressures in lumbar spine. We hypothesized that the adjacent-segment disk pressures after 1- and 2-level TDR and/or a fusion-TDR hybrid procedure will show significant variance within physiological range of motion. METHODS: Six adult spine segments T12-S1 with intact ligaments were harvested from cadavers and held firmly in a specially designed fixture. Intradiskal pressures, in motions of flexion, extension, and lateral bending, at L2-L3 and L3-L4 were measured using needle transducers after 2-level TDR L4 through S1, hybrid procedure, and 2-level fusion L4-S1 with femoral ring allograft and pedicle screws. RESULTS: The pressures with lateral bending were not significantly lower than those with flexion and extension at both levels (P = .18). Although TDR and hybrid specimens recorded slightly lower pressures specifically during lateral bending, no statistical difference in pressures could be detected when movements were combined with various procedures. CONCLUSION: Contrary to the assumed hypothesis, the pressures at the adjacent-level disks (L3-4 and L2-3) did not depend upon the stabilization procedure (2-level disk replacement, hybrid, or 2-level fusion) performed after 2-level diskectomy in the lumbosacral spine.

A biomechanical study of bankart lesion fixation - biodegradable ArthroRivet tack vs. suture repair.

Bioabsorbable fixation is commonly used in soft tissue procedures performed in the shoulder. ArthroRivettrade mark tacks (referred to as rivets here), made from a copolymer of 82% poly-L-lactic acid and 18% polyglycolic acid, were developed for the Bankart procedure. Although a previous in vivo study demonstrated favorable comparison of the fixation strength and absorption characteristics of this device with that of polyglyconate bioabsorbable tacks, there have been no published biomechanical studies of this rivet in the shoulder. Fourteen shoulders were harvested from fresh-frozen cadavers of average age 74 years (46-89). Biomechanical testing was performed by measuring the energy, or work, required to anteriorly displace the humeral head 6 mm from the glenoid. Each shoulder was tested intact, vented, and before and after repair of a simulated Bankart lesion at 0, 45, and 90 degrees of abduction with and without maximal external rotation. Overall, the average work required ranged from 54.7 N-mm to 178.27 N-mm. Although the biomechanical performance of the rivet, based on resistance to anterior displacement of the humeral head, was indistinguishable from that of the suture repair, the statistical power of the test was low due to the large variance in the cadaver specimens. The results, in general, correlated well with those of previously published studies, suggesting the suitability of the bioabsorbable rivet for use in Bankart repair.

A biomechanical study of unilateral posterior atlantoaxial transarticular screw fixation.

The purpose of this study was to investigate the fixation of C1-C2 instability with the use of a unilateral screw. Transarticular screw placement across C1-C2 may be contra-indicated in up to 20% of specimens on at least one side because of anatomic variations or other pathological processes. Hence the current study looks into unilateral screw fixation of C1- C2 instability. Eight cervical spine specimens, C1 through C5, were harvested from fresh human cadavers (4 male and 4 female) of average age 67 years (54-80). C1 and C2-C5 vertebrae were potted to allow motion only at the C1-C2 articulation. Cutting the transverse ligament on both sides of the odontoid and the tectorial membrane destabilized the specimens. Transarticular screw fixation of C1-C2 was performed in a manner similar to the technique described by Magerl. The stability was tested after fixation with one transarticular screw together with a posterior graft and wire. Placement of the screw was randomized, resulting in half the specimens receiving screws on the right side and the remaining half on the left side. The stiffness of the C1-C2 articulation was tested in rotation, lateral bending, flexion, and anterior translation in random order. The rotational stiffness was 1.44 +/- 0.44 N-m/deg, while lateral bending stiffness values were 2.33 +/- 1.14 N-m/mm (right bending) and 2.81 +/- 1.36 N-m/mm (left bending). The stiffness value in flexion was 0.813 +/- 0.189 N-m/mm and in translation 67.1 +/- 25.1 N/m. It was found that stability after unilateral transarticular screw fixation was less than that previously reported after bilateral transarticular screw fixation, but similar to that found with modified Brooks posterior wiring, which has been shown to provide better stability than other posterior wiring methods, and fusion rates of 96% have been reported. We concluded that C1-C2 unilateral posterior transarticular screw fixation with supplemental posterior graft and wiring would confer adequate stability in cases where bilateral screw placement is contraindicated.

Bioabsorbable screw fixation in coracoclavicular ligament reconstruction.

The purpose of this study is to evaluate the biomechanical properties of stainless steel and bioabsorbable screw fixation of the clavicle to the base of the coracoid. Seven matched pairs of fresh frozen shoulders were prepared by removing all soft tissue except the acromioclavicular and coracoclavicular ligament complexes. The shoulders were randomly selected and fixed with 4.5-mm stainless steel (SS) screws, while contralateral shoulders were fixed with 4.5-mm poly L-lactic acid polymer (PLLA) screws. Pullout strength, stiffness, and elongation to failure were measured using an Instron Mechanical Tester (Model 4202). The average pullout strength of 720.6 +/- 244.9 N of the metal screws was not statistically different (p = 0.089) from that of the biodegradable screws of 580.4 +/- 188.6 N. The pullout strengths exerted by both these screws exceeded the reported strength (500 N) of the intact coracoclavicular complex indicating adequate initial pullout strength for coracoclavicular fixation.

A biomechanical study of femoral neck fracture fixation with the VHS Vari-Angle Hip Fixation System.

Femoral neck fracture fixation with a new device, the VHS Vari-Angle Hip Fixation System (Biomet Orthopaedics Inc, Warsaw, IN), was studied in 14 fresh-frozen cadaver femurs. A Pauwels class III fracture was simulated in these femurs. Seven femurs were fixed by placing the screw at a high (150 degrees) angle to the femoral axis; the other 7 femurs were fixed by placing the screw at a low (135 degrees) angle to the femoral axis. Mean compressive failure load was found to be significantly higher for the high-angle group than for the low-angle group. We concluded that compared with a low-angle screw placement (135 degrees), high-angle screw placement (150 degrees) results in a biomechanically stronger fixation.

Biomechanical performance of rigid compared to dynamic anterior cervical plating: analysis of adjacent upper and lower level compressive forces.

Dynamic anterior cervical plating is well established as a means of enhancing graft loading and subsequent arthrodesis. Current concerns center on the degree of adjacent-level stress induced by these systems. The aim of this study was to evaluate and compare the load transferred to adjacent levels for single-level anterior cervical discectomy and fusion utilizing rigid compared to dynamic anterior plating systems. Nine cadaveric adult human cervical spine specimens were subjected to range-of-motion testing prior to and following C5-C6 anterior cervical discectomy and fusion procedures. Interbody grafting was performed with human fibula tissue. Nondestructive biomechanical testing included flexion/extension and lateral bending loading modes. A constant displacement of 5mm was applied in each direction and the applied load was measured in newtons (N). Specimens were tested in the following order: intact, following discectomy, after rigid plating, then after dynamic plating. Adjacent level (C4-C5 [L(S)] and C6-C7 [L(I)]) compressive forces were measured using low profile load cells inserted into each disc space. The measured load values for plating systems were then normalized using values measured for the intact specimens. Mean loads transferred to L(S) and L(I) during forced flexion in specimens with rigid plating were 23.47 N and 8.76 N, respectively; while the corresponding values in specimens with dynamic plating were 18.55 N and 1.03 N, respectively. Dynamic plating yielded no significant change at L(I) and a 21.0% decrease in load at L(S) when compared with rigid plating, although the difference was not significant. The observed trend suggests that dynamic plating may diminish superior adjacent level compressive stresses.

Biomechanics of humerus fracture fixation by locking, cortical, and hybrid plating systems in a cadaver model.

The goal of this study was to discover how locking or cortical screws or a hybrid of both would perform in stabilizing a simulated humerus fracture. We simulated stripping of screw threads or poor bone quality by overdrilling the screw hole, and also studied a control group with no overdrilling. A total of 38 fresh frozen cadaver humeri were divided into 2 groups: 16 undergoing overdrilling with a drill bit 0.3 mm less than the diameter of the screw and 22 undergoing no overdrilling. A 4-point bending test followed torsional fatigue of 1000 cycles with an amplitude of +/-10 degrees. The post-fatigued samples were retested in the same way after tightening the loose screws if necessary. Finally, each fatigued specimen was tested for failure in torsion at 0.5 Hz by applying a maximum rotational displacement of 60 degrees .The bending stiffness values (Nm/mm) of cortical and locking screws, with the exception of the hybrid system, were significantly higher for the overdrilled group than the non-overdrilled (cortical, 6.9 vs 5.6; locking, 9.1 vs 6.3; hybrid, 8.4 vs 6.8). Fatigue had no effect on the bending stiffness of all the screw/plate systems (cortical, 6.9 vs 7.4; locking, 9.1 vs 8.8; hybrid, 8.5 vs 8.1). The overdrilling had no effect on the failure loads and displacements for all the screws except cortical screws, where the failure displacement was significantly higher for the overdrilling group. The torsional stiffness retentions after 1000 cycles were significantly different for overdrilled specimens in the cortical screws group only.Overdrilling had a minimal effect on bending and torsional properties. The results of the locking and hybrid were close, and the cortical screw had only slightly lower bending stiffness.

Effect of 3D-microstructure of bioabsorbable PGA:TMC scaffolds on the growth of chondrogenic cells.

Various biomaterial scaffolds have been investigated for cartilage tissue engineering, although little attention has been paid to the effect of scaffold microstructure on tissue growth. Non-woven, fibrous, bioabsorbable scaffolds constructed from a copolymer of glycolide and trimethylene carbonate with varying levels of porosity and pore size were seeded with mesenchymal stroma cells with a chondrogenic lineage. Scaffolds and media were evaluated for both cell and extracellular matrix organization and content after up to 28 days of culture in a spinner flask. Analysis of DNA and glycosaminoglycan contents showed that the most porous of the three scaffold types, with a porosity of 81% and a porometry determined mean flow pore diameter of 54 microm, supported the most rapid proliferation of cells and accumulation of extracellular matrix. Analysis of the high porosity scaffold system, using Western Blot and immunohistochemistry confirmed the presence of collagen type II and absence of collagen type I, and demonstrated cells with a chondrocyte morphology with aggrecan and collagen II accumulation attached to the scaffolds. It was concluded that the 3D-microstructural characteristics of the scaffold (interconnecting porosity and pore size) play an important role in proliferation and phenotype of chondrogenic cells and accumulation of extracellular matrix molecules.

Traumatic spondylolisthesis of the axis: a biomechanical comparison of clinically relevant anterior and posterior fusion techniques.

OBJECT: Surgical management of unstable traumatic spondylolisthesis of the axis includes both posterior and anterior fusion methods. The authors performed a biomechanical study to evaluate the relative stability of anterior fixation at C2-3 and posterior fixation of C-1 through C-3 in hangman's fractures. METHODS: Fresh-frozen cadaveric spine specimens (occipital level to T-2) were subjected to stepwise destabilization of the C1-2 complex, replicating a Type II hangman's fracture. Intact specimens, fractured specimens, and fractured specimens with either anterior screw and plate or posterior screw and rod fixation were each tested for stability. Each spine was subjected to separate right and left rotation, bending, flexion, and extension testing. RESULTS: Anterior fixation restored stiffness in flexion and extension movements to values greater than those for intact specimens. For other movement parameters, the values approximated those for intact specimens. Posterior fixation increased the stiffness to above those values seen for anterior fixation specimens. CONCLUSIONS: In cadaveric spine specimens subjected to a Type II hangman's fracture, both anterior fixation at C2-3 and posterior fixation with C-1 lateral mass screws and C-2 and C-3 pedicle screws resulted in a consistent increase in stiffness, and hence in stability, over intact specimens.

A biomechanical study of simulated femoral neck fracture fixation by cannulated screws: effects of placement angle and number of screws.

The angle of placement of hip screws to fix femoral neck fractures is still a controversial subject, and it must be addressed. In the study reported here, we compared the relative stiffness of fixation of simulated Pauwels type III femoral neck fractures fixed with either 2 or 3 cannulated screws implanted at 135 degrees, 145 degrees, and 150 degrees. Each femur was fixed with 2 or 3 cannulated screws and tested under axial loading and anteroposterior (AP) bending. Then each femur was fatigued to 1000 cycles and tested to failure. Fourteen femurs were tested. Results showed that axial stiffness values were not statistically different at different angles. AP bending stiffness of the high-angle (150 degrees) construct was significantly higher than that of either of the other 2 constructs (for 2 screws only). Two-screw fixation appears to be adequate; adding a third screw may not be necessary.

A biomechanical study of posterior spinal instrumentation using pedicle screws with and without cross-links.

OBJECTIVE: It is not clear from the studies reported in the literature whether the addition of cross-links to bilateral pedicle screws increases the stiffness of the construct significantly to fix multilevel vertebral bodies. The current study addresses the question of how cross-links change the rotational stiffness of constructs with 6, 12, and 20 pedicle screws. METHODS: Seven thoracolumbar 10-level spine segments (T4-L1), from individuals of average age 72 years (range 46-83 years), were instrumented with pedicle screws (6, 12, and 20) and tested in torsion with and without cross-links. As the cadaver specimens had signs of osteopenia, constructs consisting of 10 wood blocks 35 x 35 x 78 mm (weight x height x length) were also tested following an identical protocol as in the cadavers to prove the concept. RESULTS: For both wood blocks and cadavers, the rotational stiffness increased linearly with number of screws with and without cross-links. Two cross-links increased rotational stiffness significantly for both 12 and 20 screws (nonsignificant for 6 screws) for wood block constructs. However, the rotational stiffness values of the cadaver constructs with or without cross-links failed to show any significant differences. CONCLUSIONS: This work has raised the need for developing a suitable animal model for the study of multilevel vertebral bodies to simulate the fixation of scoliosis corrections.

Distal tibiofibular syndesmosis fixation: a cadaveric, simulated fracture stabilization study comparing bioabsorbable and metallic single screw fixation.

Metal screws that are used for ruptured tibiofibular syndesmosis repair are often removed within 3 months of placement, suggesting the utility of bioabsorbable screws. A biomechanical study was performed to compare fixation of a simulated syndesmosis separation with a 5-mm oriented copolymer bioabsorbable (82:18 poly-L-lactic acid/poly-glycolic acid) versus a stainless steel screw. Eight pairs of cadaveric lower-leg specimens were cleaned and a pronation external rotation-type injury was created in each. The syndesmosis was fixed with a single, tricortical bioabsorbable screw in 1 ankle and a metal screw in the contralateral ankle (matched pairs). Sequential testing of the specimens showed that torsional stiffness of the fixed, relative to intact, specimens was nearly equivalent (0.730 +/- 0.260 for copolymer, 0.770 +/- 0.300 for stainless steel; P = .401). Application of 1000 cycles of axial load (90 to 900 N) resulted in a significant decrease ( P < .0001) in axial stiffness for each fixation method, but the relative decrease was equivalent for both ( P = .211). Failure torque (17.8 +/- 8.3 N.m copolymer, 21.0 +/- 11.5 N.m stainless steel; P = .238) and angle of rotation at failure (47.9 +/- 13.6 degrees copolymer, 42.0 +/- 11.5 degrees stainless steel; P = .199) were also nearly equivalent. It appears that the 5.0-mm diameter copolymer screw is biomechanically equivalent to the 5.0-mm diameter stainless steel screw for repair of syndesmosis disruption.