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1.
Nat Genet ; 38(7): 779-86, 2006 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-16804543

RESUMO

We determined the complete genome sequence of Clostridium difficile strain 630, a virulent and multidrug-resistant strain. Our analysis indicates that a large proportion (11%) of the genome consists of mobile genetic elements, mainly in the form of conjugative transposons. These mobile elements are putatively responsible for the acquisition by C. difficile of an extensive array of genes involved in antimicrobial resistance, virulence, host interaction and the production of surface structures. The metabolic capabilities encoded in the genome show multiple adaptations for survival and growth within the gut environment. The extreme genome variability was confirmed by whole-genome microarray analysis; it may reflect the organism's niche in the gut and should provide information on the evolution of virulence in this organism.


Assuntos
Clostridioides difficile/genética , Clostridioides difficile/patogenicidade , Adaptação Fisiológica , Proteínas de Bactérias/genética , Sequência de Bases , Clostridioides difficile/efeitos dos fármacos , Clostridioides difficile/fisiologia , Conjugação Genética , Elementos de DNA Transponíveis/genética , DNA Bacteriano/genética , Farmacorresistência Bacteriana Múltipla/genética , Enterocolite Pseudomembranosa/etiologia , Enterocolite Pseudomembranosa/microbiologia , Trato Gastrointestinal/microbiologia , Genoma Bacteriano , Humanos , Dados de Sequência Molecular , Mosaicismo , Análise de Sequência com Séries de Oligonucleotídeos , Esporos Bacterianos/fisiologia , Virulência/genética
2.
Indian J Microbiol ; 54(3): 284-92, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24891735

RESUMO

The human oral metagenomic DNA cloned into plasmid pUC19 was used to construct a DNA library in Escherichia coli. Functional screening of 40,000 metagenomic clones led to identification of a clone LIP2 that exhibited halo on tributyrin agar plate. Sequence analysis of LIP2 insert DNA revealed a 939 bp ORF (omlip1) which showed homology to lipase 1 of Acinetobacter junii SH205. The omlip1 ORF was cloned and expressed in E. coli BL21 (DE3) using pET expression system. The recombinant enzyme was purified to homogeneity and the biochemical properties were studied. The purified OMLip1 hydrolyzed p-nitrophenyl esters and triacylglycerol esters of medium and long chain fatty acids, indicating the enzyme is a true lipase. The purified protein exhibited a pH and temperature optima of 7 and 37 °C respectively. The lipase was found to be stable at pH range of 6-7 and at temperatures lower than 40 °C. Importantly, the enzyme activity was unaltered, by the presence or absence of many divalent cations. The metal ion insensitivity of OMLip1offers its potential use in industrial processes.

3.
Front Microbiol ; 15: 1416665, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38966395

RESUMO

Clostridioides difficile is an emerging pathogen of One Health significance. Its highly variable genome contains mobile genetic elements (MGEs) such as transposons and prophages that influence its biology. Systematic deletion of each genetic element is required to determine their precise role in C. difficile biology and contribution to the wider mobilome. Here, Tn5397 (21 kb) and ϕ027 (56 kb) were deleted from C. difficile 630 and R20291, respectively, using allele replacement facilitated by CRISPR-Cas9. The 630 Tn5397 deletant transferred PaLoc at the same frequency (1 × 10-7) as 630 harboring Tn5397, indicating that Tn5397 alone did not mediate conjugative transfer of PaLoc. The R20291 ϕ027 deletant was sensitive to ϕ027 infection, and contained two unexpected features, a 2.7 kb remnant of the mutagenesis plasmid, and a putative catalase gene adjacent to the deleted prophage was also deleted. Growth kinetics of R20291 ϕ027 deletant was similar to wild type (WT) in rich medium but marginally reduced compared with WT in minimal medium. This work indicates the commonly used pMTL8000 plasmid series works well for CRISPR-Cas9-mediated gene deletion, resulting in the largest deleted locus (56.8 kb) described in C. difficile. Removal of MGEs was achieved by targeting conjugative/integrative regions to promote excision and permanent loss. The deletants created will be useful strains for investigating Tn5397 or ϕ027 prophage contribution to host virulence, fitness, and physiology, and a platform for other mutagenesis studies aimed at functional gene analysis without native transposon or phage interference in C. difficile 630 and R20291.

4.
J Antimicrob Chemother ; 68(1): 17-22, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-22941900

RESUMO

OBJECTIVES: To identify the genes responsible for tetracycline resistance in a strain of Streptococcus australis isolated from pooled saliva from healthy volunteers in France. S. australis is a viridans Streptococcus, originally isolated from the oral cavity of children in Australia, and subsequently reported in the lungs of cystic fibrosis patients and as a cause of invasive disease in an elderly patient. METHODS: Agar containing 2 mg/L tetracycline was used for the isolation of tetracycline-resistant organisms. A genomic library in Escherichia coli was used to isolate the tetracycline resistance determinant. In-frame deletions and chromosomal repair were used to confirm function. Antibiotic susceptibility was determined by agar dilution and disc diffusion assay. RESULTS: The tetracycline resistance determinant from S. australis FRStet12 was isolated from a genomic library in E. coli and DNA sequencing showed two open reading frames predicted to encode proteins with similarity to multidrug resistance-type ABC transporters. Both genes were required for tetracycline resistance (to both the naturally occurring and semi-synthetic tetracyclines) and they were designated tetAB(46). CONCLUSIONS: This is the first report of a predicted ABC transporter conferring tetracycline resistance in a member of the oral microbiota.


Assuntos
Transportadores de Cassetes de Ligação de ATP/genética , Antiporters/genética , Proteínas de Bactérias/genética , Boca/microbiologia , Multimerização Proteica/genética , Streptococcus/genética , Resistência a Tetraciclina/genética , Transportadores de Cassetes de Ligação de ATP/química , Adulto , Antiporters/química , Proteínas de Bactérias/química , Humanos , Testes de Sensibilidade Microbiana/métodos , Valor Preditivo dos Testes , Proteínas e Peptídeos Salivares/química , Proteínas e Peptídeos Salivares/genética , Streptococcus/isolamento & purificação
5.
Microb Genom ; 9(3)2023 03.
Artigo em Inglês | MEDLINE | ID: mdl-36897935

RESUMO

The diversity of microbial insertion sequences, crucial mobile genetic elements in generating diversity in microbial genomes, needs to be better represented in current microbial databases. Identification of these sequences in microbiome communities presents some significant problems that have led to their underrepresentation. Here, we present a bioinformatics pipeline called Palidis that recognizes insertion sequences in metagenomic sequence data rapidly by identifying inverted terminal repeat regions from mixed microbial community genomes. Applying Palidis to 264 human metagenomes identifies 879 unique insertion sequences, with 519 being novel and not previously characterized. Querying this catalogue against a large database of isolate genomes reveals evidence of horizontal gene transfer events across bacterial classes. We will continue to apply this tool more widely, building the Insertion Sequence Catalogue, a valuable resource for researchers wishing to query their microbial genomes for insertion sequences.


Assuntos
Bactérias , Elementos de DNA Transponíveis , Humanos , Bactérias/genética , Biologia Computacional , Genoma Microbiano , Metagenômica
6.
J Bacteriol ; 194(8): 2125-6, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22461557

RESUMO

Here we report the draft genome sequence of Clostridium difficile strain CD37, the first nontoxigenic strain sequenced. Every sequenced strain of Clostridium difficile has been shown to contain multiple different mobile genetic elements. The draft genome sequence of strain CD37 reveals the presence of two putative conjugative transposons.


Assuntos
Clostridioides difficile/genética , Genoma Bacteriano , Sequência de Bases , Elementos de DNA Transponíveis/genética , Regulação Bacteriana da Expressão Gênica , Dados de Sequência Molecular
7.
Appl Environ Microbiol ; 78(7): 2147-53, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22267673

RESUMO

The insertion sites of the conjugative transposon Tn916 in the anaerobic pathogen Clostridium difficile were determined using Illumina Solexa high-throughput DNA sequencing of Tn916 insertion libraries in two different clinical isolates: 630ΔE, an erythromycin-sensitive derivative of 630 (ribotype 012), and the ribotype 027 isolate R20291, which was responsible for a severe outbreak of C. difficile disease. A consensus 15-bp Tn916 insertion sequence was identified which was similar in both strains, although an extended consensus sequence was observed in R20291. A search of the C. difficile 630 genome showed that the Tn916 insertion motif was present 100,987 times, with approximately 63,000 of these motifs located in genes and 35,000 in intergenic regions. To test the usefulness of Tn916 as a mutagen, a functional screen allowed the isolation of a mutant. This mutant contained Tn916 inserted into a gene involved in flagellar biosynthesis.


Assuntos
Proteínas de Bactérias/genética , Clostridioides difficile/genética , Conjugação Genética , Elementos de DNA Transponíveis/genética , Mutagênese Insercional , Bacillus subtilis/genética , Proteínas de Bactérias/metabolismo , Sequência de Bases , Mapeamento Cromossômico , Clostridioides difficile/classificação , Clostridioides difficile/isolamento & purificação , Clostridioides difficile/ultraestrutura , DNA Bacteriano/genética , Flagelos/metabolismo , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Mutação , Ribotipagem , Especificidade da Espécie
8.
Mol Oral Microbiol ; 37(4): 133-153, 2022 08.
Artigo em Inglês | MEDLINE | ID: mdl-35674142

RESUMO

The objective of this review was to assess the prevalence of antibiotic resistance genes in the oral cavity and identify mobile genetic elements (MGEs) important in disseminating them. Additionally, to assess if age, geographic location, oral site, bacterial strains and oral disease influence the prevalence of these genes. Three electronic databases (Medline, Embase and the Cochrane Library) were used to search the literature. Journals and the grey literature were also hand searched. English language studies from January 2000 to November 2020 were selected. Primary screening was performed on the titles and abstracts of 1509 articles generated. One hundred and forty-seven full texts were obtained to conduct the second screening with strict inclusion and exclusion criteria. Forty-four final articles agreed with the inclusion criteria. Half of the studies were classed as low quality. tet(M) was the most prevalent gene overall and the conjugative transposon Tn916 the most common MGE associated with antibiotic resistance genes in the oral cavity. In babies delivered vaginally, tet(M) was more prevalent, whilst tet(Q) was more prevalent in those delivered by C-section. Generally, countries with higher consumption of antibiotics had higher numbers of antibiotic resistance genes. Agricultural as well as medical use of antibiotics in a country should always be considered. Between healthy, periodontitis and peri-implantitis subjects, there was no difference in the prevalence of tet(M); however, erm(B), tet(M) and tet(O) were higher in carious active children than the non-carious group. Subjects with poor oral hygiene have more pathogenic bacteria that carry resistance genes compared to those with good oral hygiene. Enterococcus faecalis isolates demonstrated significant tetracycline resistance (tet(M) up to 60% prevalence in samples) and erythromycin resistance (erm(B) up to 61.9% prevalence in samples), periodontal pathogens showed significant beta-lactam resistance with blaZ and cfxA present in up to 90%-97% of samples and the normal oral flora had a high level of erythromycin resistance with mef(A/E) present in 65% of Streptococcus salivarius isolates. The most common resistance gene was tet(M) in root canals, cfxA in subgingival plaque, erm(B) in supragingival plaque and tet(W) in 100% of whole saliva samples. The review highlights that although many studies in this area have been performed, 50% were classed as low quality. We advise the following recommendations to allow firm conclusions to be drawn from future work: the use of large sample sizes, investigate a broad range of antibiotic resistance genes, improved methodologies and reporting to improve the quality of genetic testing in microbiology and randomisation of subject selection.


Assuntos
Antibacterianos , Farmacorresistência Bacteriana , Antibacterianos/farmacologia , Bactérias/genética , Criança , Farmacorresistência Bacteriana/genética , Eritromicina , Humanos , Sequências Repetitivas Dispersas/genética , Testes de Sensibilidade Microbiana , Boca , Prevalência
9.
J Antimicrob Chemother ; 66(10): 2235-9, 2011 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-21816764

RESUMO

OBJECTIVES: Tn916-like elements are one of the most common types of integrative and conjugative element (ICE). In this study we aimed to determine whether novel accessory genes, i.e. genes whose products are not involved in mobility or regulation, were present on a Tn916-like element (Tn6087) isolated from Streptococcus oralis from the human oral cavity. METHODS: A minocycline-resistant isolate was analysed using restriction fragment length polymorphism (RFLP) analysis on amplicons derived from Tn916 and DNA sequencing to determine whether there were genetic differences in Tn6087 compared with Tn916. Mutational analysis was used to determine whether the novel accessory gene found was responsible for an observed extra phenotype. RESULTS: A novel Tn916-like element, Tn6087, is described that encodes both antibiotic and antiseptic resistance. The antiseptic resistance protein is encoded by a novel small multidrug resistance gene, designated qrg, that was shown to encode resistance to cetyltrimethylammonium bromide (CTAB), also known as cetrimide bromide. CONCLUSIONS: This is the first Tn916-like element described that confers both antibiotic and antiseptic resistance, suggesting that selection of either antibiotic or antiseptic resistance will also select for the other and further highlights the need for prudent use of both types of compound.


Assuntos
Antibacterianos/farmacologia , Anti-Infecciosos Locais/farmacologia , Farmacorresistência Bacteriana Múltipla/genética , Sequências Repetitivas Dispersas , Streptococcus oralis/efeitos dos fármacos , Streptococcus oralis/genética , Proteínas de Bactérias/genética , Sequência de Bases , Cetrimônio , Compostos de Cetrimônio/farmacologia , Conjugação Genética , DNA Bacteriano/genética , Transferência Genética Horizontal , Humanos , Testes de Sensibilidade Microbiana , Minociclina/farmacologia , Mutação , Polimorfismo de Fragmento de Restrição , Análise de Sequência de DNA , Resistência a Tetraciclina/genética
10.
Trends Microbiol ; 29(2): 158-170, 2021 02.
Artigo em Inglês | MEDLINE | ID: mdl-32448763

RESUMO

There has been an explosion of metagenomic data representing human, animal, and environmental microbiomes. This provides an unprecedented opportunity for comparative and longitudinal studies of many functional aspects of the microbiome that go beyond taxonomic classification, such as profiling genetic determinants of antimicrobial resistance, interactions with the host, potentially clinically relevant functions, and the role of mobile genetic elements (MGEs). One of the most important but least studied of these aspects are the MGEs, collectively referred to as the 'mobilome'. Here we elaborate on the benefits and limitations of using different metagenomic protocols, discuss the relative merits of various sequencing technologies, and highlight relevant bioinformatics tools and pipelines to predict the presence of MGEs and their microbial hosts.


Assuntos
Bactérias/genética , Microbiota , Animais , Bactérias/classificação , Bactérias/isolamento & purificação , Humanos , Sequências Repetitivas Dispersas , Metagenoma , Metagenômica
11.
Antimicrob Agents Chemother ; 54(11): 4924-6, 2010 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-20713671

RESUMO

Antibiotic-resistant Enterococcus faecalis and Clostridium difficile are responsible for nosocomial infections in humans, in which they inhabit the same niche. Here, we demonstrate transfer of the conjugative transposon Tn5397 from C. difficile 630 to E. faecalis JH2-2, the first reported gene transfer between these two bacteria. Furthermore, transfer from the E. faecalis EF20A transconjugant to the epidemic ribotype 027 C. difficile strain R20291 was also demonstrated. Tn5397 was shown to use a single specific target site in E. faecalis; it also has specific target sites in C. difficile. These experiments highlight the importance of continual monitoring for emerging resistances in these bacteria.


Assuntos
Clostridioides difficile/genética , Elementos de DNA Transponíveis/genética , Enterococcus faecalis/genética , Transferência Genética Horizontal/genética
12.
Nat Commun ; 11(1): 693, 2020 02 04.
Artigo em Inglês | MEDLINE | ID: mdl-32019923

RESUMO

The global threat of antimicrobial resistance has driven the use of high-throughput sequencing techniques to monitor the profile of resistance genes, known as the resistome, in microbial populations. The human oral cavity contains a poorly explored reservoir of these genes. Here we analyse and compare the resistome profiles of 788 oral cavities worldwide with paired stool metagenomes. We find country and body site-specific differences in the prevalence of antimicrobial resistance genes, classes and mechanisms in oral and stool samples. Within individuals, the highest abundances of antimicrobial resistance genes are found in the oral cavity, but the oral cavity contains a lower diversity of resistance genes compared to the gut. Additionally, co-occurrence analysis shows contrasting ARG-species associations between saliva and stool samples. Maintenance and persistence of antimicrobial resistance is likely to vary across different body sites. Thus, we highlight the importance of characterising the resistome across body sites to uncover the antimicrobial resistance potential in the human body.


Assuntos
Bactérias/genética , Farmacorresistência Bacteriana , Intestinos/microbiologia , Boca/microbiologia , Antibacterianos/farmacologia , Bactérias/classificação , Bactérias/efeitos dos fármacos , Bactérias/isolamento & purificação , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Biodiversidade , Fezes/microbiologia , Microbioma Gastrointestinal , Humanos , Metagenoma , Filogenia
13.
Sci Rep ; 10(1): 20709, 2020 11 26.
Artigo em Inglês | MEDLINE | ID: mdl-33244063

RESUMO

Antibiotic resistance poses an increasing threat to global health. To tackle this problem, the identification of principal reservoirs of antibiotic resistance genes (ARGs) plus an understanding of drivers for their evolutionary selection are important. During a PCR-based screen of ARGs associated with integrons in saliva-derived metagenomic DNA of healthy human volunteers, two novel variants of genes encoding a D-alanine-D-alanine ligase (ddl6 and ddl7) located within gene cassettes in the first position of a reverse integron were identified. Treponema denticola was identified as the likely host of the ddl cassettes. Both ddl6 and ddl7 conferred high level resistance to D-cycloserine when expressed in Escherichia coli with ddl7 conferring four-fold higher resistance to D-cycloserine compared to ddl6. A SNP was found to be responsible for this difference in resistance phenotype between the two ddl variants. Molecular dynamics simulations were used to explain the mechanism of this phenotypic change at the atomic scale. A hypothesis for the evolutionary selection of ddl containing integron gene cassettes is proposed, based on molecular docking of plant metabolites within the ATP and D-cycloserine binding pockets of Ddl.


Assuntos
Ciclosserina/farmacologia , Farmacorresistência Bacteriana/genética , Integrons/genética , Peptídeo Sintases/genética , Antibacterianos/farmacologia , Escherichia coli/efeitos dos fármacos , Humanos , Simulação de Acoplamento Molecular , Treponema denticola/efeitos dos fármacos
14.
J Bacteriol ; 191(8): 2764-75, 2009 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-19181800

RESUMO

Integrative and conjugative elements (ICEs), also called conjugative transposons, are genomic islands that excise, self-transfer by conjugation, and integrate in the genome of the recipient bacterium. The current investigation shows the intraspecies conjugative transfer of the first described ICEs in Streptococcus thermophilus, ICESt1 and ICESt3. Mitomycin C, a DNA-damaging agent, derepresses ICESt3 conjugative transfer almost 25-fold. The ICESt3 host range was determined using various members of the Firmicutes as recipients. Whereas numerous ICESt3 transconjugants of Streptococcus pyogenes and Enterococcus faecalis were recovered, only one transconjugant of Lactococcus lactis was obtained. The newly incoming ICEs, except the one from L. lactis, are site-specifically integrated into the 3' end of the fda gene and are still able to excise in these transconjugants. Furthermore, ICESt3 was retransferred from E. faecalis to S. thermophilus. Recombinant plasmids carrying different parts of the ICESt1 recombination module were used to show that the integrase gene is required for the site-specific integration and excision of the ICEs, whereas the excisionase gene is required for the site-specific excision only.


Assuntos
Conjugação Genética , Transferência Genética Horizontal , Sequências Repetitivas Dispersas , Streptococcus thermophilus/genética , Alquilantes/farmacologia , Dano ao DNA , Enterococcus faecalis/genética , Lactococcus lactis/genética , Mitomicina/farmacologia , Recombinação Genética , Streptococcus pyogenes/genética
15.
Antimicrob Agents Chemother ; 53(1): 273-6, 2009 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-18955517

RESUMO

tet(32) Was identified in three bacterial isolates and in metagenomic DNA from the human oral cavity. The regions immediately flanking the gene were found to have similarities to the mobile elements TnB1230 from Butyrivibrio fibrisolvens, ATE-3 from Arcanobacterium pyogenes, and CTn5 from Clostridium difficile.


Assuntos
Boca/microbiologia , Proteínas Repressoras/genética , Arcanobacterium/efeitos dos fármacos , Arcanobacterium/genética , Butyrivibrio/efeitos dos fármacos , Butyrivibrio/genética , Clostridioides difficile/efeitos dos fármacos , Clostridioides difficile/genética , Elementos de DNA Transponíveis/genética , Elementos de DNA Transponíveis/fisiologia , Modelos Genéticos , Dados de Sequência Molecular , Filogenia , Proteínas Repressoras/classificação
16.
Sci Rep ; 9(1): 8388, 2019 06 10.
Artigo em Inglês | MEDLINE | ID: mdl-31182805

RESUMO

Integrons are genetic elements consisting of a functional platform for recombination and expression of gene cassettes (GCs). GCs usually carry promoter-less open reading frames (ORFs), encoding proteins with various functions including antibiotic resistance. The transcription of GCs relies mainly on a cassette promoter (PC), located upstream of an array of GCs. Some integron GCs, called ORF-less GCs, contain no identifiable ORF with a small number shown to be involved in antisense mRNA mediated gene regulation. In this study, the promoter activity of ORF-less GCs, previously recovered from the oral metagenome, was verified by cloning them upstream of a gusA reporter, proving they can function as a promoter, presumably allowing bacteria to adapt to multiple stresses within the complex physico-chemical environment of the human oral cavity. A bi-directional promoter detection system was also developed allowing direct identification of clones with promoter-containing GCs on agar plates. Novel promoter-containing GCs were identified from the human oral metagenomic DNA using this construct, called pBiDiPD. This is the first demonstration and detection of promoter activity of ORF-less GCs from Treponema bacteria and the development of an agar plate-based detection system will enable similar studies in other environments.


Assuntos
Genes , Metagenoma , Boca/microbiologia , Fases de Leitura Aberta/genética , Regiões Promotoras Genéticas , Ensaios Enzimáticos , Glucuronidase/metabolismo , Humanos , Integrons/genética , Plasmídeos/genética
17.
Sci Rep ; 9(1): 15330, 2019 10 25.
Artigo em Inglês | MEDLINE | ID: mdl-31653906

RESUMO

The major global pathogen Clostridium difficile (recently renamed Clostridioides difficile) has large genetic diversity including multiple mobile genetic elements. In this study, whole genome sequencing of 86 strains from the poorly characterised clade 3, predominantly PCR ribotype (RT)023, of C. difficile revealed distinctive surface architecture characteristics and a large mobile genetic island. These strains have a unique sortase substrate phenotype compared with well-characterised strains of C. difficile, and loss of the phage protection protein CwpV. A large genetic insertion (023_CTnT) comprised of three smaller elements (023_CTn1-3) is present in 80/86 strains analysed in this study, with genes common among other bacterial strains in the gut microbiome. Novel cargo regions of 023_CTnT include genes encoding a sortase, putative sortase substrates, lantibiotic ABC transporters and a putative siderophore biosynthetic cluster. We demonstrate the excision of 023_CTnT and sub-elements 023_CTn2 and 023_CTn3 from the genome of RT023 reference strain CD305 and the transfer of 023_CTn3 to a non-toxigenic C. difficile strain, which may have implications for the use of non-toxigenic C. difficile strains as live attenuated vaccines. Finally, we show that the genes within the island are expressed in a regulated manner in C. difficile RT023 strains conferring a distinct "niche adaptation".


Assuntos
Membrana Celular/metabolismo , Clostridioides difficile/genética , Elementos de DNA Transponíveis/genética , Sequência de Aminoácidos , Anaerobiose , Proteínas de Bactérias/química , Proteínas de Bactérias/metabolismo , Sequência de Bases , Parede Celular/metabolismo , Genes Bacterianos , Ilhas Genômicas , Humanos , Microbiota/genética , Peptídeo Hidrolases/química , Peptídeo Hidrolases/metabolismo
18.
Plasmid ; 60(3): 167-73, 2008 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-18778731

RESUMO

Transposable DNA elements occur naturally in the genomes of nearly all species of prokaryotes. A proposal for a uniform transposable element nomenclature was published prominently in the 1970s but is not, at present, available online even in abstract form, and many of the newly discovered elements have been named without reference to it. We propose here an updated version of the original nomenclature system for all of the various types of prokaryotic, autonomous, transposable elements excluding insertion sequences, for which a nomenclature system already exists. The use of this inclusive and sequential Tn numbering system for transposable elements, as described here, recognizes the ease of interspecies spread of individual elements, and allows for the naming of mosaic elements containing segments from two or more previously described types of transposons or plasmids. It will guard against any future need to rename elements following changes in bacterial nomenclature which occurs constantly with our increased understanding of bacterial phylogenies and taxonomic groupings. It also takes into account the increasing importance of metagenomic sequencing projects and the continued identification of new mobile elements from unknown hosts.


Assuntos
Elementos de DNA Transponíveis/genética , Terminologia como Assunto
19.
FEMS Microbiol Lett ; 364(21)2017 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-29029255

RESUMO

Broad host range conjugative plasmids that replicate in Escherichia coli have been widely used to mobilise smaller replicons, bearing their cognate origin of transfer (oriT) into a variety of organisms that are less tractable genetically, such as Clostridium (Clostridioides) difficile. In this work we demonstrated that the oriT region of pMTL9301 (derived from RK2) is not required for transfer between E. coli and C. difficile strains 630Δerm and CD37 and that this oriT-independent transfer is abolished in the presence of DNase when CD37 is the recipient. Transfer to the 630Δerm strain is DNase resistant even without an obvious oriT, when E. coli CA434 is used as a donor and is sensitive to DNase when E. coli HB101 is the donor.


Assuntos
Clostridioides difficile/genética , Conjugação Genética , Desoxirribonucleases/metabolismo , Escherichia coli/genética , Plasmídeos/genética , DNA/metabolismo , Elementos de DNA Transponíveis , Transferência Genética Horizontal
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