[Hemoglobin components in normal and anemic b/b rats]. / Komponente hemoglobina u normalnih i anemicnih b/b pacova.

The analitical comparison of normal and anemic b/b rat hemoglobins was made by polyacrylamide gel electrophoresis (PGE) nad also by DEAE-cellulose chromatography. Six different hemoglobin components constitute total hemoglobin of normal rat. The component six of total hemoglobin (Hb VI) of controls was absent from the hemolysates of anemic animals. This could be demonstrated either by PGE or by DEAE-cellulose chromatography. Hb V was also missing to a verying degree in some of anemic animal.

Preincubation of synaptosomes in the presence of sodium affects Ca2+ uptake.

Preincubation of synaptosomes in standard physiological medium stimulates 2-fold Ca2+ uptake as compared to non-preincubated synaptosomes. When the sodium concentration in the preincubation medium has been halved, Ca2+ uptake was reduced by approximately 50 percent. The addition of ouabain to the preincubation medium decreases depolarization-stimulated Ca2+ uptake by about 40 percent. A steady-state level of Ca2+ uptake is achieved by synaptosomes preincubated for 0, 5 or 10 min. These findings suggest that Ca2+ uptake might depend on the Na-gradient formed during the preincubation of synaptosomes under control conditions.