RESUMO
In this article modern representations about cellular mechanisms of formation by pharmacological preparations of urgent protection of organs against damage are given at transplantation. On an example of ischemic damage of kidneys it is shown, that at use of preparations of different pharmacological groups by the most expressed protective effect those from them which operating within the limits of a nonspecific adaptive syndrome of cellular systems, clearly induce in organs the evolutional developed signs of cytogenetic recapitulation possess: support a cellular homeostasis at the lowered level at the expense of activation of a glycolysis and conformational reorganizations of macromolecules, and also change in cells of water contain- decrease of free and increase of bound.
Assuntos
Citoproteção , Preservação Biológica/métodos , Substâncias Protetoras/uso terapêutico , Transplantes , Animais , HumanosRESUMO
INTRODUCTION: Recently, more and more attracted the attention of cell therapy, which requires a study of the efficacy and safety of allogeneic MSCs transplantation in acute and chronic inflammatory reactions. The aim of our study was to examine the effectiveness of transplantation of allogeneic mesenchymal bone marrow stromal cells for the healing of surgical wounds the glandular stomach in rats. MATERIAL AND METHODS: Using white Wistar rats. Producing cell transplantation mononuclear fraction derived from rat bone marrow aspirate. Injected cells 8 and 9 th passage. The dose of cells administered to 3-th and / or 7-th days 3,5h106 cells / ml twice or 5,0h106 cells / ml dose. Autopsy on day 10-th and 17-th. The serum ELISA determined the content of the pro- and anti-inflammatory cytokines IL1ß, TNFα, IFNy, IL-4. RESULTS AND DISCUSSION: Introduction MSCs contributed to the decline of the immune mediators of inflammation IL1P, TNFa, IFNy, increase anti-inflammatory IL4. Quality improved healing. CONCLUSION: Rapid curative effect of stem cells may be associated with the formation of blood immune cells (macrophages) that produce substances that restore damaged tissue. They restore the balance between Th1 and Th2.
Assuntos
Mucosa Gástrica , Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais/imunologia , Gastropatias , Aloenxertos , Animais , Células da Medula Óssea/imunologia , Células da Medula Óssea/patologia , Citocinas/imunologia , Mucosa Gástrica/imunologia , Mucosa Gástrica/lesões , Mucosa Gástrica/patologia , Células-Tronco Mesenquimais/patologia , Ratos , Ratos Wistar , Gastropatias/imunologia , Gastropatias/patologia , Gastropatias/terapia , Células Th1/imunologia , Células Th1/patologia , Células Th2/imunologia , Células Th2/patologiaRESUMO
On an experimental model of chronic fibrotic liver damage (male rats Wistar (n-60), damage of CCl4, the duration of the experiment 90 days) it was studied the effectiveness of cell therapy for the correction of chronic liver failure. These rats were divided into 3 experimental groups: in the Ist-group (control, n=10) isotonic saline (650 mkl.) was injected; in the IInd-group (n=20) suspension of liver cells was applicated in a dose 8 - l0 x 10(6) cells; in the IIIrd-group (n=30) suspension of liver cells and bone marrow cells (mesenchymal stromal cells) in ratio 5:1 were used as cell associates on microparticles intjectable heterogeneous biopolymer hydrogel "SpheroGEL" (cell-engineering design) in common dose 8 - l0 x 10(6) It was ascertained that in the 2nd and in the 3rd groups the accelerated normalization of disturbed liver functional indices (ALT, AST, ALP) took place - to 30 days, but in the control group only to 90 days. The reliable differences in rats ofnormalization offunctional indices were absent between the IInd and the IIIrd groups. But in 90 days by using special histological dyeing it was found out that defibrotic processes in liver tissue were more expressed in the IIIrd group in comparison with the IIIrd group. Received results were consequence of prolonged vital activity of cells (liver cells and mesenchymal stromal bone marrow cells) into cell-engineering designs, which were transplanted in the IIIrd group. The obtained effect can be explained by that the developed cell-engineering designs provide adequate conditions for prolonged vital activity of the transplanted cells.
Assuntos
Engenharia Celular/métodos , Transplante de Células/métodos , Doença Hepática Terminal/cirurgia , Hepatócitos/transplante , Animais , Modelos Animais de Doenças , Doença Hepática Terminal/patologia , Seguimentos , Hepatócitos/citologia , Masculino , Ratos , Ratos Wistar , SuspensõesRESUMO
Transplantation of mesenchymal stem cells, both at early and later stage after local exposure of rats source beta radiation dose, 90Sr/90Y 140 GR, stimulates recovery of damaged skin. Diminution area local radiation injuries and accelerate healing radiation ulcers. Clinically shows the high efficiency of the transplantations autologous mesenchymal stem cells in treatment of deep beam ulcers, intractable standard conservative treatment. Found promising application of mesenchymal stem cells for treatment of severe local radiation injuries and the need to develop the best possible conditions for their use.
Assuntos
Transplante de Células-Tronco Mesenquimais , Lesões Experimentais por Radiação/terapia , Radiodermite/terapia , Pele/lesões , Idoso , Animais , Feminino , Raios gama/efeitos adversos , Humanos , Masculino , Pessoa de Meia-Idade , Lesões Experimentais por Radiação/patologia , Radiodermite/patologia , Ratos , Ratos Wistar , Pele/patologia , Transplante AutólogoRESUMO
It is shown that multipotent mesenchymal stromal cells (MMSC) grafted to the zone of ulcer defect contribute to better wound repair in case of chronic non-healing gastric ulcers. It is noted that precultivative MMSC isolated from bone marrow achieve their bioregulatory activity in patient's organism not at once but due to prolonged functioning in the grafting zone (not less than 30 days).
Assuntos
Células da Medula Óssea , Transplante de Células-Tronco Mesenquimais , Células-Tronco Multipotentes/transplante , Úlcera Gástrica/terapia , Animais , Autoimunidade , Doença Crônica , Interpretação Estatística de Dados , Modelos Animais de Doenças , Masculino , Ratos , Úlcera Gástrica/diagnóstico , Úlcera Gástrica/imunologia , Fatores de Tempo , Transplante Autólogo , Resultado do Tratamento , CicatrizaçãoRESUMO
The influence of cultivated multipotent mesenchymal stromal cells (MMSC) of the autologous bone marrow on activity of wound healing processes was studied using the model of long-indolent autoimmune gastric ulcer in rats. MMSC of the bone marrow when under the state of stress inhibition do not possess optimal and regulatory activity. Precultivated MMSC of the bone marrow accelerate the processes of regeneration of the gastric ulcer. MMSC of the bone marrow realize their bioregulatory
Assuntos
Células da Medula Óssea/citologia , Transplante de Células-Tronco Mesenquimais/métodos , Úlcera Gástrica/cirurgia , Células Estromais/citologia , Cicatrização/fisiologia , Animais , Células Cultivadas , Modelos Animais de Doenças , Seguimentos , Masculino , Ratos , Ratos Wistar , Úlcera Gástrica/patologia , Transplante AutólogoRESUMO
The efficiency of correction of cytokine disbalance and peptic ulcer regeneration has been studied using the model of chronic peptic ulcer at use based on mononuclear fraction cells (MFC) of autologous bone marrow (BM). MFCs are shown to remove cytokine disbalance, reduce systemic inflammation reaction and accelerate peptic ulcer regeneration.
Assuntos
Doenças Autoimunes/cirurgia , Células da Medula Óssea/imunologia , Transplante de Medula Óssea/métodos , Citocinas/imunologia , Regeneração/imunologia , Úlcera Gástrica/cirurgia , Animais , Doenças Autoimunes/patologia , Células da Medula Óssea/citologia , Citocinas/sangue , Modelos Animais de Doenças , Masculino , Monócitos/imunologia , Monócitos/transplante , Ratos , Ratos Wistar , Úlcera Gástrica/imunologia , Úlcera Gástrica/patologia , Transplante Autólogo , Resultado do TratamentoRESUMO
This review describes up-to-date concepts of the type I diabetes mellitus pathogenesis and its complications. The leading role of the immune system malfunction in pancreatic islet of Langerhans and in vessel wall regeneration impairment is emphasized. It is suggested, that cell therapy of the type I diabetes mellitus with pancreatic islet and bone marrow cell transplantation promote beta-cell regeneration due to normalization of intracellular interaction in these tissues and immune homeostasis in whole organism.
Assuntos
Transplante de Medula Óssea/métodos , Complicações do Diabetes/terapia , Diabetes Mellitus Tipo 1/terapia , Células Secretoras de Insulina/transplante , Complicações do Diabetes/prevenção & controleRESUMO
The aim of the investigation was to study a possibility to improve left ventricular (LV) volume and function remodelling in patients with chronic cardiac insufficiency (CCI) by means oftwo-stage bone marrow cell (BMC) activation: first in vivo and then ex vivo within the process of their cultivation. Two groups of CCI patients were recruited. Group 1 (controls) consisted of 50 patients undergoing conventional aorto-coronary bypass surgery (ACBS). Group 2 consisted of 57 patients injected with 200 million autological mononuclear BMC intramyocardially during ACBS. In Group 2 patients, the severity of immune dysregulation was assessed initially using blood leukocyte stimulation index (SI) values. Sixteen patients with SI >1 and moderate immunographic alterations were considered to have a favorable prognosis for BMC treatment; 41 subjects with SI <1 and pronounced immunographic abnormalities were regarded as having an unfavorable prognosis for BMC application. Twenty-two patients with SI <1 were administered a preliminary immunocorrective course (in vivo BMC activation). Mononuclear BMC obtained from 38 patients with SI >1 and 19 patients with SI <1 were cultured for 5 to 7 days (ex vivo BMC activation). Positive changes in BMC phenotypical pattern were observed only in patients with SI >1: CD3, CD4, CD8, CD25, and some other measurements increased. Significant positive effects on LV function parameters and Duke Activity Status Index (DASI) values were revealed in patients with SI >1 six months after BMC administration. In vivo immunocorrection in combination with subsequent ex vivo BMC activation (n=38) promoted significant improvements in LV volume characteristics 6 months after ACBS vs. the controls (ACBS without BMC, n=50). In conclusion, to make the administration of autological BMC in CCI patients effective, two-staged BMC activation should be performed: in vivo activation with immunocorrectors should be followed by ex vivo activation of cultured cells.
Assuntos
Células da Medula Óssea/imunologia , Transplante de Medula Óssea/métodos , Insuficiência Cardíaca/fisiopatologia , Imunidade Celular/imunologia , Linfócitos T/imunologia , Função Ventricular Esquerda/fisiologia , Remodelação Ventricular/fisiologia , Células da Medula Óssea/citologia , Transplante de Medula Óssea/imunologia , Volume Cardíaco/fisiologia , Células Cultivadas , Seguimentos , Insuficiência Cardíaca/imunologia , Insuficiência Cardíaca/cirurgia , Humanos , Pessoa de Meia-Idade , Transplante Autólogo , Resultado do TratamentoRESUMO
The authors suggest using chemiluminescent reaction of the patients' blood leukocytes including the stimulation index (SI) for prediction of the body resistance to stress damage (operative stress). It is shown that in chronic cardiac patients (n = 259) SI is initially subnormal. Some of the above patients had critically low SI ( > 0.6). Such patients developed severe immunodependent postoperative complications. To increase the patients' resistance to operative stress and to avoid postoperative complications it is recommended to conduct prophylactic immunocorrecting therapy under SI control.
Assuntos
Adaptação Fisiológica , Leucócitos/metabolismo , Estresse Oxidativo , Complicações Pós-Operatórias , Espécies Reativas de Oxigênio/metabolismo , Estresse Fisiológico/metabolismo , Humanos , Prognóstico , Estresse Fisiológico/sangue , Estresse Fisiológico/etiologiaRESUMO
Diabetes mellitus type 1 (DM-1) was modeled by a single intraperitoneal injection of streptozotocine (STZ) in a dose 65 mg/kg in two groups of Wistar rats: control (n=6) and test (n=14). Clinical and pathomorphological examinations of different organs were conducted for 4.5 months. It was found that the above model represents clinical picture and vascular diabetic complications (microangiopathy with parenchymal lesions) accompanied with destruction and depression of spleen activity. It is shown that rats, more resistant to the toxic action of STZ restore the ability for regeneration of the pancreatic islets and regress of glycemia and angiopathy, for positive changes in splenic structure and function.
Assuntos
Complicações do Diabetes/patologia , Diabetes Mellitus Experimental/complicações , Diabetes Mellitus Tipo 1/complicações , Modelos Animais de Doenças , Ratos , Animais , Diabetes Mellitus Experimental/induzido quimicamente , Diabetes Mellitus Tipo 1/induzido quimicamente , Masculino , Ratos Wistar , Estreptozocina/toxicidadeRESUMO
Coronary artery bypass grafting (CABG) has been made in two groups of patients with chronic heart failure (CHF) with further estimation of the rate of postoperative organic dysfunctions and pyoseptic complications. In group 1 (n = 50) CABG was combined with intracoronary or intramyocardial injection of autologous precultivated for 7-8 days mononuclear cells of the bone marrow (1 x 10(9) cells). In group 2 (n = 479) the intraoperative injection of the above cells was not made. It was found that autologous cultivated mononuclear bone marrow cells prevent organic dysfunction and reduce frequency of infectious-septic complications especially in patients with preoperative focuses of chronic infections.
Assuntos
Células da Medula Óssea/citologia , Transplante de Medula Óssea/métodos , Procedimentos Cirúrgicos Cardíacos , Insuficiência de Múltiplos Órgãos , Complicações Pós-Operatórias , Sepse , Adulto , Idoso , Humanos , Pessoa de Meia-Idade , Insuficiência de Múltiplos Órgãos/epidemiologia , Insuficiência de Múltiplos Órgãos/imunologia , Insuficiência de Múltiplos Órgãos/prevenção & controle , Sepse/epidemiologia , Sepse/imunologia , Sepse/prevenção & controle , Transplante Autólogo , Resultado do TratamentoRESUMO
The article is dedicated to the pathogenetic mechanisms of the development of immune inflammation in the vascular wall, as well as hepatic functional disregulation in dislipidemia and atherosclerosis. The authors emphasize that the decompensation of the process of cell regeneration in actively functioning organs (vascular endothelium, hepatocytes) and a concomitant immune disregulation become the main factors of the progression of dislipidemia and atherosclerosis. The authors consider the conditions for and factors of cell therapy, based on the use of allogenic fetal hepatic cells and autological bone marrow cells.
Assuntos
Aterosclerose/terapia , Transplante de Medula Óssea , Dislipidemias/terapia , Hepatócitos/transplante , Animais , Aterosclerose/etiologia , Aterosclerose/imunologia , Aterosclerose/fisiopatologia , Medula Óssea , Células da Medula Óssea/fisiologia , Células Cultivadas , Dieta Aterogênica , Modelos Animais de Doenças , Progressão da Doença , Dislipidemias/etiologia , Dislipidemias/imunologia , Dislipidemias/fisiopatologia , Endotélio Vascular/fisiologia , Endotélio Vascular/fisiopatologia , Seguimentos , Humanos , Fígado/embriologia , Fígado/fisiopatologia , Regeneração Hepática , Coelhos , Regeneração , Fatores de Tempo , Transplante HomólogoRESUMO
The authors analyze the pathogenetic significance of inflammation, immune activation, and apoptosis in the development of heart failure and the functional regeneration of left ventricular myocardium after treatment including autologic bone marrow cell transplantation. The paper shows that autologic bone marrow cell transplantation influences the same mechanisms that cause heart failure, namely inflammation, apoptosis, and neurohumoral mechanisms. Investigations have made it possible to formulate guidelines for the prediction and monitoring of the effects of treatment including autologic bone marrow cell transplantation in patients with heart failure.
Assuntos
Apoptose/fisiologia , Transplante de Medula Óssea , Citocinas/sangue , Insuficiência Cardíaca , Ventrículos do Coração/patologia , Insuficiência Cardíaca/sangue , Insuficiência Cardíaca/patologia , Insuficiência Cardíaca/cirurgia , Humanos , Resultado do TratamentoRESUMO
Transplantation of suspention of allogenic fibroblast-like mesenchymal stem cells (AFMSC) of the bone marrow and AFMSC immobilized on the biodegradable membrane (BM) stimulates healing of deep burn wounds compared to control (without cell transplantation) because these cells produce biologically active substances into the wound. AFMSC immobilized on BM activate repair processes in the wounds earlier. This may be due to their higher functional activity created in the monolayer by adequate intercellular interactions. To avoid complications caused by low diffuse properties of BM (protein precipitation), BM should be removed from the surface of the burn wound maximum 3 days after its application.
Assuntos
Materiais Biocompatíveis/metabolismo , Queimaduras/terapia , Células Imobilizadas , Células-Tronco Mesenquimais , Transplante de Células-Tronco/métodos , Animais , Células da Medula Óssea/citologia , Queimaduras/patologia , Técnicas de Cultura de Células/instrumentação , Técnicas de Cultura de Células/métodos , Fibroblastos/citologia , Masculino , Células-Tronco Mesenquimais/citologia , Ratos , Ratos WistarRESUMO
A comparative analysis of the impact of cell therapy (allogenic fetal liver cells, stem cells of autologic bone marrow) on lipid homeostasis, microcirculatory disturbances and structural changes in some organs was made using the atherosclerosis model in guinea-pigs. A positive effect of cell therapy on these parameters was established. The effect was stronger and prolonged after transplantation of stem cells. Atherogenic diet weakens the effect of cell therapy. Thus, cell therapy is more effective in restriction of atherogenic risk factors.
Assuntos
Transplante de Medula Óssea/métodos , Transplante de Células/métodos , Dislipidemias/terapia , Animais , Aterosclerose/patologia , Aterosclerose/terapia , Dieta Aterogênica , Dislipidemias/patologia , Cobaias , Fígado/embriologia , Fígado/patologia , Masculino , MicrocirculaçãoRESUMO
An experimental study on rat hearts has demonstrated that cardioplegic solutions, apart from their protective effect, activate energy-consuming calcium redistribution process. Presented data permit qualitative and quantitative assessment of myocardial changes and a prompt evaluation of arrested heart's functional status.
Assuntos
Parada Cardíaca Induzida , Coração/efeitos dos fármacos , Soluções Isotônicas/toxicidade , Compostos de Potássio , Potássio/toxicidade , Animais , Cálcio/metabolismo , Coração/fisiopatologia , Parada Cardíaca Induzida/efeitos adversos , Potenciais da Membrana/efeitos dos fármacos , Contração Miocárdica/efeitos dos fármacos , Ratos , Fatores de TempoRESUMO
An experimental study on rat hearts using continuous recording of myocardial tension showed that the addition of various groups of drugs to the solution had negative effects on heart function. Positive effect was achieved when cardioplegia was combined with the pretreatment of animals with anti-ischemic drugs (alpha-tocopherol, dimethylsulfoxide) as well as preliminary oxygenation of the medium. Possible mechanisms of these myocardial effects are discussed.
Assuntos
Doença das Coronárias/prevenção & controle , Dimetil Sulfóxido , Parada Cardíaca Induzida , Oxigênio , Vitamina E , Animais , RatosRESUMO
An experimental study in 307 isolated rat hearts was concerned with variations in myocardial resting tension, resting membrane potential, ATP levels and myocardial heat production in conditions of cardioplegic heart arrest by an ion-balanced cardioplegic solution with and without phosphorylated metabolites (ATP, phosphocreatine, inosine-F, glucose-6-phosphate) and dephosphorylated metabolites (glucose, inosine, and inorganic phosphates). Phosphorylated metabolites, incorporated in the solution, had a marked protective effect due to the activation of substrate regulation mechanisms at different stages of ATP synthesis and facilitation of cardiomyocyte adaptation to ischemic, hypothermic and cardioplegic exposure.
Assuntos
Trifosfato de Adenosina/farmacologia , Soluções Cardioplégicas/farmacologia , Doença das Coronárias/prevenção & controle , Parada Cardíaca Induzida , Fosfocreatina/farmacologia , Animais , Sinergismo Farmacológico , Técnicas In Vitro , Masculino , RatosRESUMO
Hemoperfusion via donor hepatocytes and splenic fragments is an effective adjuvant method of treatment of hepatic insufficiency, especially in patients with autoimmune disease. Hemoperfusion effect occurs due to normalization of mesenchymoinflammatory and immunological indices as a result of positive action of hepatotropic and immunotropic factors of the spleen. The highest effectiveness of the above hemoperfusion is reached in its early use, that is before the disease becoming chronic.