Spatial variation in iodine content with relation to soil physicochemical properties in lower Himalayan region.

Topography of a place has a significant impact on soil characteristics that ultimately influence soil iodine levels. Lower Himalayan region (LHR) in Pakistan has a wide range of climatic and geological variations. Hence, an investigation was conducted to analyze the iodine concentration and other physicochemical properties of soils in two LHR districts, Haripur and Mansehra. Spatial analysis indicated a decrease in iodine levels in the mountainous regions in comparison to the flat portions of LHR. Soil samples obtained from different locations across Haripur had a stronger affinity for iodine due to variations in solubility and adsorption of iodine to soil clay components, which can be attributed to lower pH, higher organic matter, and a higher cation exchange capacity (CEC). In contrast to the plains of Haripur, elevated locations in the Mansehra district had decreased levels of iodine, along with a higher soil pH and reduced soil organic matter. The soil erosion and depletion of soil micronutrients in the hilly region of Mansehra may be attributed to the unfavorable soil conditions and excessive precipitation. Presence of clay, iron (Fe), and aluminum (Al) in the soil led to a rise in iodine levels. Iodine concentrations exhibited an inverse relationship with soil acidity. Study revealed a direct correlation between soil iodine levels and their cation exchange capacity (CEC) and clay content. This study aims to gather fundamental data for the chosen regions of LHR to address illnesses caused by iodine deficiency.

Impact of microplastic pollution on coastal ecosystems using comprehensive beach quality indices.

Previous studies of Microplastics (Mps) pollution focused on abundance, effect on organisms, and origins. Mps could also be indicators to evaluate pollution level. Beach Quality Indices (BQIs) are useful in understanding Mps pollution level. This study is to assess magnitude, impact and quality of beaches using BQIs, by determining abundance, shape, and size of Mps in beach sediments, which is the first effort in China. Three BQIs, i.e. Microplastic Pollution Index (MPPI), Environmental Status Index (ESI), Coefficient of Microplastic Impact (CMPI), were employed involving Sector Analysis Approach. All beaches had "very high" abundance by MPPI, were classified "bad" by ESI, and fell in "red" sector using Sector Analysis Approach by intergradation of MPPI and ESI. The impact of fiber morphology was "extreme" based on CMPI. The average abundance was 664±80 Mps/kg. Fibers occupied >97 % of Mps, with 31 % of black Mps. A model was proposed to determine Mps origins.

Spatio-temporal variations of litter on Qingdao tourist beaches in China.

Beaches are an integral part of coastal tourism, but they are deteriorated by the beachgoers and recreational activities due to lack of adequate beach environmental awareness and management. Litter is widely distributed in marine and coastal environment and has been considered a severe concern. In China investigations to determine the beach litter abundance and pollution level are limited. The aim of this study is to estimate spatio-temporal distribution and composition of litter on 10 well-known Qingdao tourist beaches, involving pollution level by beach quality indexes. Beach litter was collected within an area of 25 × 25 m2 in both summer (May, June and July) and winter (Nov, Dec and Jan) seasons, and was classified into eight categories. The abundance of beach litter was found higher in summer (0.13 ± 0.04 items/m2) than in winter (0.04 ± 0.01 items/m2). Overall, the percentage of plastics were higher in both summer (23.48%) and winter (24.04%) than that of other litter categories. Based on Clean Coast Index, 70% of beaches were very clean, 25% clean, and 5% moderately clean. Beach Grade Index showed that 15% beaches were very good, 5% good, 55% fair, and 25% poor. 85% beaches constituted some quantity of hazardous litter and 15% had no hazardous litter for Hazardous Items index. The findings suggest that the sources of beach litter along Qingdao beaches mainly come from the recreational and tourist activities. The substantial quantity of litter is also being transported by ocean (tides or current), which are finally deposited along beachfront. Despite regular cleaning operation along most of Qingdao beaches, suggested management practices involve mitigation measures, source reduction, change in littering behavior to improve further quality of beaches.

Microplastic distribution within core sediments of beach and its responses to anthropogenic activities.

Nowadays, people have known more about the distribution features on the surface of beaches. However, the understanding on the microplastics (Mps) distribution in the vertical direction on beaches is still lacking. This study analysed the vertical distribution of Mps from five beaches, with one long core [Shilaoren Beach (C1), 8 m] and four short cores [Aoshan Beach (C2); Liuqinghewan Beach (C3); Bathing Beach No. 3 (C4); and Golden Beach (C5); approximately 70 cm]. An increasing trend of Mps from bottom to top layers were observed with an average of 16.6 ± 4.8 Mps/25 g d.w. in C1. Conversely, a fluctuating trend was found in C2-C5, with an average Mps abundance on the 70 cm depth to be 6.7, 7.1, 11.1 and 7.0 Mps/25 g d.w., respectively. These Mps were mainly comprised of fibres (>98%) and few were fragments. Mps were not uniformly distributed within all cores, and this variation was due to changes in Mps sources, hydrodynamics and sedimentation process in the beach environment. Although the vertical accumulation of Mps is not as stable as the other sedimentary environments, it still presents the increasing trend in the 8-m core from the bottom to the top, which is consistent with the plastic production history in China since 1950. Therefore, the 8-m core has been deposited on the beach in the recent 70 years. Thus, this study provides a valuable example for tracing the sedimentation history on the beach, which can help in understanding the sediment deposit and transport processes with time on beaches.

In Silico Prospects and Therapeutic Applications of Ouabagenin and Hydroxylated Corticosteroid Analogues in the Treatment of Lung Cancer.

Lung cancer is the second most prevalent carcinoma around the world, and about 80% of patients are of non-small cell lung cancer (NS-CLC). Epidermal growth factor receptor (EGFR) is the most expressed protein kinases in lung cancer and hence can be used in target-related anti-cancer therapy. Here, computational approach is used for the exploration of the anti-cancer potential of new steroid derivatives as previously no in vitro data was available for them. Initially, DFT calculations of all compounds were determined to analyze the electronic density of optimized structures. The HOMO and LUMO orbital analysis of all derivatives was analyzed, to investigate the reactivity of compounds. Afterwards, optimized structures were used for molecular docking studies in which all ouabagenin derivatives were docked within the EGFR active site using MOE software. Moreover, anti-cancer potential of selected derivatives was evaluated on the basis of binding interactions with three anti-cancer proteins. The binding scores of these compounds were compared with the FDA-approved drug, i.e., gefitinib. The findings of current study suggested that selected derivatives exhibited significant inhibiting potential of anti-cancer proteins and EGFR. Particularly, compound OD3 is the potent inhibitor of anti-cancer and EGFR protein with the highest binding energies. These novel steroidal derivatives are subjected to in silico analysis for the first time against lung cancer. These compounds possess potential anti-cancerous properties and can be explored further for in vitro and in vivo studies.

In-silico Investigations of quinine and quinidine as potential Inhibitors of AKR1B1 and AKR1B10: Functional and structural characterization.

The aberrant expression of aldo keto reductases (AKR1B1 & AKR1B10) has been extensively studied in different types of cancer especially the colon cancer but a very few studies have yet been reported regarding the discovery of inhibitors for the treatment of colon cancer by targeting these isozymes. Therefore, there is a need of selective inhibitors of both targets for the eradication of colon cancer. Currently, the study is focused on the exploration of two quinolone compounds i.e., (S)-(6-Methoxyquinolin-4-yl)[(1S,2R,4S,5R)-5-vinylquinuclidin-2-yl]methanol (Quinidine) and (R)-(6-Methoxyquinolin-4-yl)[(1S,2S,4S,5R)-5-vinylquinuclidin-2-yl]methanol (Quinine) as the potential inhibitors of AKR1B1 and AKR1B10 via detailed in-silico approach. The structural properties including vibrational frequencies, dipole moment, polarizability and the optimization energies were estimated using density functional theory (DFT) calculations; where both compounds were found chemically reactive. After that, the optimized structures were used for the molecular docking studies and here quinidine was found more selective towards AKR1B1 and quinine exhibited maximum inhibition of AKR1B10. The results of molecular docking studies were validated by molecular dynamics simulations which provided the deep insight of stability of protein ligand complex. At the end, the ADMET properties were determined to demonstrate the druglikeness properties of both selected compounds. These findings suggested further exploration of both compounds at molecular level using different in-vivo and in-vitro approaches that will lead to the designing of potential inhibitor of AKR1B1/AKR1B10 for curing colon cancer and related malignancies.