RESUMO
Here, we present a protocol for the development of mRNA-loaded lipid nanoparticle (LNP) vaccines for target antigen sequences of interest. We describe key steps required to design and synthesize mRNA constructs, their LNP encapsulation, and mouse immunization. We then detail quality control assays to determine RNA purity, guidelines to measure RNA immunogenicity using in vitro reporter systems, and a technique to evaluate antigen-specific T cell responses following immunization.
Assuntos
Imunização , Lipídeos , Nanopartículas , RNA Mensageiro , Animais , Camundongos , Nanopartículas/química , RNA Mensageiro/genética , Lipídeos/química , Imunização/métodos , Vacinas/imunologia , Vacinas/química , Vacinas/administração & dosagem , Vacinas de mRNA/imunologia , Nanovacinas , LipossomosRESUMO
Transposable elements (TEs), which make up almost half of the human genome, often display altered expression in cancers. Here, we review recent progress in elucidating the role of TEs as mediators of immune responses in cancer and discuss how novel therapeutic strategies can harness TE immunogenicity for cancer immunotherapy.