Emergence of Inducible Macrolide Resistance in Mycobacterium chelonae Due to Broad-Host-Range Plasmid and Chromosomal Variants of the Novel 23S rRNA Methylase Gene, erm(55).

Macrolides are a mainstay of therapy for infections due to nontuberculous mycobacteria (NTM). Among rapidly growing mycobacteria (RGM), inducible macrolide resistance is associated with four chromosomal 23S rRNA methylase (erm) genes. Beginning in 2018, we detected high-level inducible clarithromycin resistance (MICs of ≥16µg/mL) in clinical isolates of Mycobacterium chelonae, an RGM species not previously known to contain erm genes. Using whole-genome sequencing, we identified a novel plasmid-mediated erm gene. This gene, designated erm(55)P, exhibits <65% amino acid identity to previously described RGM erm genes. Two additional chromosomal erm(55) alleles, with sequence identities of 81% to 86% to erm(55)P, were also identified and designated erm(55)C and erm(55)T. The erm(55)T is part of a transposon. The erm(55)P allele variant is located on a putative 137-kb conjugative plasmid, pMchErm55. Evaluation of 133 consecutive isolates from 2020 to 2022 revealed 5 (3.8%) with erm(55). The erm(55)P gene was also identified in public data sets of two emerging pathogenic pigmented RGM species: Mycobacterium iranicum and Mycobacterium obuense, dating back to 2008. In both species, the gene appeared to be present on plasmids homologous to pMchErm55. Plasmid-mediated macrolide resistance, not described previously for any NTM species, appears to have spread to multiple RGM species. This has important implications for antimicrobial susceptibility guidelines and treatment of RGM infections. Further spread could present serious consequences for treatment of other macrolide-susceptible RGM. Additional studies are needed to determine the transmissibility of pMchErm55 and the distribution of erm(55) among other RGM species.

The effects of race and criminal history on landlords' (un)willingness to rent to exonerees.

OBJECTIVE: When wrongfully convicted individuals are released from prison, at first glance, it is a triumph; however, anecdotal evidence from exonerees suggests that obtaining housing postrelease is often challenging. We empirically examined whether race (Study 1) or type of criminal offense (Study 2) influenced landlords' willingness to rent to exonerees compared to releasees (i.e., rightfully convicted individuals released from prison) and control (i.e., members of the public). HYPOTHESES: We hypothesized that: (a) exonerees and releasees would receive fewer replies and fewer "yes" available responses compared to control, (b) Indigenous and Black renters would receive fewer replies and fewer "yes" available responses compared to White renters, and (c) individuals convicted of murder would receive fewer replies and fewer "yes" available responses compared to individuals convicted of robbery. METHOD: The authors responded to online apartment listings across Canada (Study 1) and in Toronto (Study 2) inquiring about unit availability. All rental inquiries were identical with the exception of criminal status and race (Study 1), and criminal status and criminal offense (Study 2). RESULTS: Results demonstrated that landlords were significantly less likely to respond (Study 1: OR = 4.32, 95% CI [3.28, 5.69]; Study 2: OR = 7.88, 95% CI [4.97, 12.48]), and indicate availability (Study 1: OR = 6.62, 95% CI [3.54, 12.38]; Study 2: OR = 21.53, 95% CI [7.07, 65.58]), to rental inquiries from exonerees and releasees compared to members of the public. For race, landlords were significantly less likely to respond to inquiries from Indigenous and Black renters compared to White renters (OR = 1.45, 95% CI [1.12, 1.86]), and those convicted of robbery compared to murder (OR = 1.69, 95% CI [.36, .97]). CONCLUSION: The barriers that exonerees face when attempting to secure housing postrelease are potentially as great as those facing releasees; however, exonerees do not receive assistance with reentry. (PsycInfo Database Record (c) 2020 APA, all rights reserved).

The complete mitochondrial genome of the file ramshorn snail Planorbella pilsbryi (Mollusca: Gastropoda: Hygrophila: Planorbidae).

The file ramshorn snail Planorbella pilsbryi Baker, 1926 (Gastropoda: Hygrophila: Planorbidae) is a widespread herbivorous North American freshwater snail found in diverse habitats, including standing and moving water bodies. Genome skimming by Illumina sequencing allowed the assembly of a complete nuclear rRNA repeat sequence and a complete circular mitogenome of 13,720 bp from P. pilsbryi consisting of 75.3% AT nucleotides, 22 tRNAs, 13 protein-coding genes, 2 rRNAs and a control region in the typical order found in panpulmonate snails. Planorbella pilsbryi COXI features a rare TTG start codon while COXII, CYTB, ND2, ND3, and ND5 exhibit incomplete stop codons completed by the addition of 3' A residues to the mRNA. Phylogenetic reconstruction of mitochondrial protein-coding gene and rRNA sequences places P. pilsbryi as sister taxon to Planorbella duryi (Planorbidae) within family Planorbidae, which is consistent with previous phylogenetic hypotheses.