Skin colour, carotenogenesis and chlorophyll degradation mutant alleles: genetic orchestration behind the fruit colour variation in tomato.

The genetics underlying the fruit colour variation in tomato is an interesting area of both basic and applied research in plant biology. There are several factors, like phytohormones, environmental signals and epistatic interactions between genes, which modulate the ripe fruit colour in tomato. However, three aspects: genetic regulation of skin pigmentation, carotenoid biosynthesis and ripening-associated chlorophyll degradation in tomato fruits are of pivotal importance. Different genes along with their mutant alleles governing the aforementioned characters have been characterized in detail. Moreover, the interaction of these mutant alleles has been explored, which has paved the way for developing novel tomato genotypes with unique fruit colour and beneficial phytonutrient composition. In this article, we review the genes and the corresponding mutant alleles underlying the variation in tomato skin pigmentation, carotenoid biosynthesis and ripening-associated chlorophyll degradation. The possibility of generating novel fruit colour-variants using different combinations of these mutant alleles is documented. Furthermore, the involvement of some other mutant alleles (like those governing purple fruit colour and high fruit pigmentation), not belonging to the aforementioned three categories, are discussed in brief. The simplified representation of the assembled information in this article should not only help a broad range of readers in their basic understanding of this complex phenomenon but also trigger them for further exploration of the same. The article would be useful for genetic characterization of fruit colour-variants and molecular breeding for fruit colour improvement in tomato using the well-characterized mutant alleles.

Spatio-temporal regulation of the OsHFP gene promoter establishes the involvement of this protein in rice anther development.

Anther development leading to pollen maturation, anther dehiscence and pollen dispersal depends upon the precise timing of programmed cell death (PCD) in specified anther tissues. The PCD necessitates a properly tuned transcriptional regulation of some crucial genes. However, the detailed genetic regulation of this PCD in rice anther is yet to be deciphered. Recently, we have established that the OsHFP, a structurally novel hemopexin fold protein of rice is a flower-specific heme binding protein, and plays a role in chlorophyll degradation. Here, we report the spatio-temporal transcriptional regulation of the OsHFP gene, which is proposed to be involved in anther PCD. The OsHFP was immunodetected in rice anthers, and OsHFP-related proteins were also found to be present in anthers of other monocot (lily) and dicot (tobacco) plant species. Unique cis-acting elements, possibly involved in the activation and anther-specificity of the OsHFP promoter were identified based upon in silico prediction and in planta expression profiling of the reporter gene driven by the OsHFP promoter (2051 bp) and its two deleted versions (1057 bp and 437 bp). The temporal regulation of the OsHFP promoter in different developmental stages of tobacco anther implies the physiological function of this protein in anther PCD.

Development of a transgenic hairy root system in jute (Corchorus capsularis L.) with gusA reporter gene through Agrobacterium rhizogenes mediated co-transformation.

Transgenic hairy root system is important in several recalcitrant plants, where Agrobacterium tumefaciens-mediated plant transformation and generation of transgenic plants are problematic. Jute (Corchorus spp.), the major fibre crop in Indian subcontinent, is one of those recalcitrant plants where in vitro tissue culture has provided a little success, and hence, Agrobacterium-mediated genetic transformation remains to be a challenging proposition in this crop. In the present work, a system of transgenic hairy roots in Corchorus capsularis L. has been developed through genetic transformation by Agrobacterium rhizogenes harbouring two plasmids, i.e. the natural Ri plasmid and a recombinant binary vector derived from the disarmed Ti plasmid of A. tumefaciens. Our findings indicate that the system is relatively easy to establish and reproducible. Molecular analysis of the independent lines of transgenic hairy roots revealed the transfer of relevant transgenes from both the T-DNA parts into the plant genome, indicating the co-transformation nature of the event. High level expression and activity of the gusA reporter gene advocate that the transgenic hairy root system, thus developed, could be applicable as gene expression system in general and for root functional genomics in particular. Furthermore, these transgenic hairy roots can be used in future as explants for plantlet regeneration to obtain stable transgenic jute plants.