Complexin-2 redistributes to the membrane of muscle cells in response to insulin and contributes to GLUT4 translocation.

Insulin stimulates glucose uptake in muscle cells by rapidly redistributing vesicles containing GLUT4 glucose transporters from intracellular compartments to the plasma membrane (PM). GLUT4 vesicle fusion requires the formation of SNARE complexes between vesicular VAMP and PM syntaxin4 and SNAP23. SNARE accessory proteins usually regulate vesicle fusion processes. Complexins aide in neuro-secretory vesicle-membrane fusion by stabilizing trans-SNARE complexes but their participation in GLUT4 vesicle fusion is unknown. We report that complexin-2 is expressed and homogeneously distributed in L6 rat skeletal muscle cells. Upon insulin stimulation, a cohort of complexin-2 redistributes to the PM. Complexin-2 knockdown markedly inhibited GLUT4 translocation without affecting proximal insulin signalling of Akt/PKB phosphorylation and actin fiber remodelling. Similarly, complexin-2 overexpression decreased maximal GLUT4 translocation suggesting that the concentration of complexin-2 is finely tuned to vesicle fusion. These findings reveal an insulin-dependent regulation of GLUT4 insertion into the PM involving complexin-2.

Different epidemiological profiles in patients with Zika and dengue infection in Tapachula, Chiapas in Mexico (2016-2018): an observational, prospective cohort study.

BACKGROUND: The introduction of Zika and chikungunya to dengue hyperendemic regions increased interest in better understanding characteristics of these infections. We conducted a cohort study in Mexico to evaluate the natural history of Zika infection. We describe here the frequency of Zika, chikungunya and dengue virus infections immediately after Zika introduction in Mexico, and baseline characteristics of participants for each type of infection. METHODS: Prospective, observational cohort evaluating the natural history of Zika virus infection in the Mexico-Guatemala border area. Patients with fever, rash or both, meeting the modified criteria of PAHO for probable Zika cases were enrolled (June 2016-July 2018) and followed-up for 6 months. We collected data on sociodemographic, environmental exposure, clinical and laboratory characteristics. Diagnosis was established based on viral RNA identification in serum and urine samples using RT-PCR for Zika, chikungunya, and dengue. We describe the baseline sociodemographic and environmental exposure characteristics of participants according to diagnosis, and the frequency of these infections over a two-year period immediately after Zika introduction in Mexico. RESULTS: We enrolled 427 participants. Most patients (n = 307, 65.7%) had an acute illness episode with no identified pathogen (UIE), 37 (8%) Zika, 82 (17.6%) dengue, and 1 (0.2%) chikungunya. In 2016 Zika predominated, declined in 2017 and disappeared in 2018; while dengue increased after 2017. Patients with dengue were more likely to be men, younger, and with lower education than those with Zika and UIE. They also reported closer contact with water sources, and with other people diagnosed with dengue. Participants with Zika reported sexual exposure more frequently than people with dengue and UIE. Zika was more likely to be identified in urine while dengue was more likely found in blood in the first seven days of symptoms; but PCR results for both were similar at day 7-14 after symptom onset. CONCLUSIONS: During the first 2 years of Zika introduction to this dengue hyper-endemic region, frequency of Zika peaked and fell over a two-year period; while dengue progressively increased with a predominance in 2018. Different epidemiologic patterns between Zika, dengue and UIE were observed. Trial registration Clinical.Trials.gov (NCT02831699).

Efficacy of transcutaneous perineal electrostimulation versus intracavitary anal electrostimulation in the treatment of urinary incontinence after a radical prostatectomy: Randomized controlled trial.

AIM: To compare the efficacy of the treatment with transcutaneous perineal electrostimulation versus intracavitary electrostimulation to reduce the frequency of urinary incontinence after radical prostatectomy and the impact on the quality of life (QoL). METHODS: This single-blind equivalence-randomized controlled trial equally (1:1) randomly allocated men with urinary incontinence post radical prostatectomy into surface electrodes perineal group (intervention group, IG) and intra-anal probe group (control group, CG). Outcomes included changes in the 24h-Pad Test (main variable), and ICIQ-SF (International Consultation on Incontinence Questionnaire Short-Form), SF-12 (Short Form Health Survey), and I-QOL (incontinence quality of life questionnaire) questionnaires. Clinical data were collected at baseline, 6 and 10 weeks. For the comparisons between variables, χ2 test and Student's t test were used. Equivalence was analyzed by estimating the mean change (90% confidence interval) of urinary incontinence based on the Pad Test. The analysis was performed for the per-protocol and the intention-to-treat populations. Statistical significance level was set at p < 0.05. RESULTS: Seventy patients were included, mean age 62.8 (SD 9.4) years. Mean baseline 24h-Pad Test was 328.3 g (SD 426.1) and a significant decrease (p < 0.001) in the grams of urine loss at 5 weeks (159.1 g in the IG and 121.7 g in the CG), and at 10 weeks of treatment (248.5 g in the IG and 235.8 g in the CG) was observed. However, the final difference in the grams of urine loss between both treatments showed the absence of statistical significance (p = 0.874). In both groups, the ICIQ-SF, I-QOL, and SF-12 questionnaires revealed a significant improvement in QoL. CONCLUSION: Surface and intra-anal electrostimulation treatments reduced significantly losses of urine, but differences in grams of urine loss throughout the therapy between groups were not significant, suggesting that the efficacy of the two treatments is not statistically different. Nonetheless, the improvement observed in both groups was statistically significant and clinically relevant.

Benefits of a Pediatric Antimicrobial Stewardship Program in Antimicrobial Use and Quality of Prescriptions in a Referral Children's Hospital.

OBJECTIVES: To evaluate the results of the first 24 months of a postprescription review with feedback-based antimicrobial stewardship program in a European referral children's hospital. STUDY DESIGN: We performed a pre-post study comparing antimicrobial use between the control (2015-2016) and the intervention periods (2017-2018) expressed in days of therapy/100 days present. Quality of prescriptions was evaluated by quarterly cross-sectional point-prevalence surveys. Length of stay, readmission rates, in-hospital mortality rates, cost of systemic antimicrobial agents, and antimicrobial resistance rates were included as complementary outcomes. RESULTS: Total antimicrobial use and antibacterial use significantly decreased during the intervention period (P = .002 and P = .001 respectively), and total antifungal use remained stable. A significant decline in parenteral antimicrobial use was also observed (P < .001). In 8 quarterly point-prevalence surveys (938 prescriptions evaluated), the mean prevalence of use of any antimicrobial among inpatients was 39%. An increasing trend in the rate of optimal prescriptions was observed after the first point-prevalence survey (P = .0898). Nonoptimal prescriptions were more common in surgical than in medical departments, in antibacterial prescriptions with prophylactic intention, and in empirical more than in targeted treatments. No significant differences were observed in terms of mortality or readmission rates. Only minor changes in antimicrobial resistance rates were noted. CONCLUSIONS: Our antimicrobial stewardship program safely decreased antimicrobial use and expenditure, and a trend toward improvement in quality of prescription was also observed.

Sickness presenteeism: Are we sure about what we are studying? A research based on a literature review and an empirical illustration.

BACKGROUND: There has been an increasing interest in studying sickness presenteeism (SP). An ever-increasing amount of scientific literature is published using this term, yet there appears to be considerable heterogeneity in how it is assessed, which could result in substantial differences in the definition and interpretation of the phenomenon really being studied. We aim to discuss what really is being studied, depending on how the phenomenon is operationalized, measured, and analyzed. METHODS: A study based on a literature review and an empirical illustration using data of the third Spanish Psychosocial Risks Survey (2016). RESULTS: Differences are observed based on the population in which SP is measured, the cut-off points used to define a worker as presenteeist, the reasons for an SP episode and even an analysis of the phenomenon treated as a count or as a dichotomous. CONCLUSIONS: Without being completely exclusive, it seems that restricting the population of analysis to only those workers who consider that they should not have gone to work due to their health, and/or establishing low cut-off points to define someone as presenteeist, would more clearly delimit the study of SP to the exercise of a right to sick leave. In contrast, working with the entire population or using high cut-off points appears to relate the study of SP more with health status and less with the exercise of rights. On the other hand, taking the reasons for SP into account would probably help to improve interpretation of the phenomenon.

Specific psychosocial exposures for workers' mental health: A population-based study.

BACKGROUND: In order to plan interventions it is important to obtain evidence on the relation between a health outcome and specific exposures. However, there are few studies that identify the effect of specific psychosocial work exposures on poor mental health. This is the aim of this study. METHODS: Population-based cross-sectional study in Catalonia. We estimated prevalence ratios (PR) of minor psychiatric disorder associated with several psychosocial work exposures. RESULTS: The items with highest PR were "Are there times when you need to be at work and at home at the same time?" (PR = 1.81), "Are you worried about a variation in your salary?" (PR = 1.77), "Is your work emotionally demanding?" (PR = 1.65) and "Are you worried about it being difficult for you to find another job if you became unemployed?" (PR = 1.51). CONCLUSIONS: This study could be useful in order to begin planning interventions on specific psychosocial exposures to protect mental health in the workplace.

Occupational exposure assessment of highway toll station workers to vehicle engine exhaust.

Toll station workers are occupationally exposed to vehicle engine exhaust, a complex mixture of different chemical substances, including carcinogenic compounds. Therefore, a study was carried out on attendants of two highway toll stations to describe their occupational exposure to vehicle engine exhaust, based on a worst-case scenario approach. Personal sampling was conducted during the day shift for all attendants, testing for three groups of chemical substances: polycyclic aromatic hydrocarbons (PAHs), volatile organic compounds (VOCs) and aldehydes (formaldehyde and acrolein). Concentrations of total PAH, BTEX (benzene, toluene, ethylbenzene, and xylenes) and formaldehyde content varied between 97.60-336.08 ng/m3, 5.01-40.52 µg/m3, and 0.06-19.13 µg/m3, respectively. No clear relationships could be established between exposure levels and the number of vehicles. Furthermore, no differences were found between truck versus car lanes, or inside versus outside the tollbooth. Not all the detected VOCs were related to vehicle exhaust; some were consistent with the use of cleaning products. The measured concentrations were far below the established occupational exposure limits, but tended to be higher than values reported for outdoor urban environments. There are very few international studies assessing occupational exposures among toll station workers, and this is the first such study to be conducted in Spain. The results suggest that further, more detailed studies are necessary to characterize exposure properly, and ones which include other airborne pollutants, such as ultrafine particles. The comparison of the results to other similar studies was difficult, since no data related to some important exposure determinants have been provided. Therefore, it is recommended that these determinants be considered in future studies.

Flow cytometry protocol for GLUT4-myc detection on cell surfaces.

Insulin and muscle contraction trigger GLUT4 translocation to the plasma membrane, which increases glucose uptake by muscle cells. Insulin resistance and Type 2 diabetes are the result of impaired GLUT4 translocation. Quantifying GLUT4 translocation is essential for comprehending the intricacies of both physiological and pathophysiological processes involved in glucose metabolism. The most commonly used methods for measuring GLUT4 translocation are the ELISA-type assay and the immunofluorescence assay. While some reports suggest that flow cytometry could be useful in quantifying GLUT4 translocation, this technique is not frequently used. Much of our current understanding of the regulation of GLUT4 has been based on experiments using the rat myoblast cell line (L6 cell) which expresses GLUT4 with a myc epitope on the exofacial loop. In the present study, we use the L6-GLUT4myc cell line to develop a flow cytometry-based approach to detect GLUT4 translocation. Flow cytometry offers the advantages of both immunofluorescence and ELISA-based assays. It allows easy identification of separate cell populations in the sample, similar to immunofluorescence, while providing results based on a population-level analysis of multiple individual cells, like an ELISA-based assay. Our results demonstrate a 0.6-fold increase with insulin stimulation compared with basal conditions. Finally, flow cytometry consistently yielded results across different experiments and exhibited sensitivity under the tested conditions.

The light chain of tetanus toxin bound to arginine-rich cell-penetrating peptide inhibits cortical reaction in mouse oocytes.

Introduction: Cortical reaction is a secretory process that occurs after a spermatozoon fuses with the oocyte, avoiding the fusion of additional sperm. During this exocytic event, the cortical granule membrane fuses with the oocyte plasma membrane. We have identified several molecular components involved in this process and confirmed that SNARE proteins regulate membrane fusion during cortical reaction in mouse oocytes. In those studies, we microinjected different nonpermeable reagents to demonstrate the participation of a specific protein in the cortical reaction. However, the microinjection technique has several limitations. In this work, we aimed to assess the potential of cell-penetrating peptides (CPP) as biotechnological tools for delivering molecules into oocytes, and to evaluate the functionality of the permeable tetanus toxin (bound to CPP sequence) during cortical reaction. Methods: Arginine-rich cell-penetrating peptides have demonstrated the optimal internalization of small molecules in mammalian cells. Two arginine-rich CPP were used in the present study. One, labeled with 5-carboxyfluorescein, to characterize the factors that can modulate its internalization, and the other, the permeable light chain of tetanus toxin, that cleaves the SNAREs VAMP1 and VAMP3 expressed in mouse oocytes. Results: Results showed that fluorescent CPP was internalized into the oocyte cytoplasm and that internalization was dependent on the concentration, time, temperature, and maturation stage of the oocyte. Using our functional assay to study cortical reaction, the light chain of tetanus toxin bound to arginine-rich cell-penetrating peptide inhibited cortical granules exocytosis. Discussion: Results obtained from the use of permeable peptides demonstrate that this CPP is a promising biotechnological tool to study functional macromolecules in mouse oocytes.

Candida albicans internalization by host cells is mediated by a clathrin-dependent mechanism.

Candida albicans is a major cause of oropharyngeal, vulvovaginal and haematogenously disseminated candidiasis. Endocytosis of C. albicans hyphae by host cells is a prerequisite for tissue invasion. This internalization involves interactions between the fungal invasin Als3 and host E- or N-cadherin. Als3 shares some structural similarity with InlA, a major invasion protein of the bacterium Listeria monocytogenes. InlA mediates entry of L. monocytogenes into host cells through binding to E-cadherin. A role in internalization, for a non-classical stimulation of the clathrin-dependent endocytosis machinery, was recently highlighted. Based on the similarities between the C. albicans and L. monocytogenes invasion proteins, we studied the role of clathrin in the internalization of C. albicans. Using live-cell imaging and indirect immunofluorescence of epithelial cells infected with C. albicans, we observed that host E-cadherin, clathrin, dynamin and cortactin accumulated at sites of C. albicans internalization. Similarly, in endothelial cells, host N-cadherin, clathrin and cortactin accumulated at sites of fungal endocytosis. Furthermore, clathrin, dynamin or cortactin depletion strongly inhibited C. albicans internalization by epithelial cells. Finally, beads coated with Als3 were internalized in a clathrin-dependent manner. These data indicate that C. albicans, like L. monocytogenes, hijacks the clathrin-dependent endocytic machinery to invade host cells.

Prevalence, associated factors and reasons for sickness presenteeism: a cross-sectional nationally representative study of salaried workers in Spain, 2016.

OBJECTIVES: The aim of this study was to estimate the prevalence of sickness presenteeism (SP), its associated factors and the reasons given for SP episodes, among the overall salaried population and excluding the 'healthy' workers. DESIGN: Population-based cross-sectional study. SETTING: Salaried population in Spain. PARTICIPANTS: Data were obtained from the third Spanish Psychosocial Risks Survey (2016), carried out between October and December 2016, n=1615. MAIN OUTCOME MEASURES: Self-reported episodes of SP and their reasons. RESULTS: 23.0% (95% CI 19.2 to 26.8) of the workers exhibit SP, whereas among those manifesting having had some health problem in the preceding year, the figure was 53.0% (95% CI 46.9 to 59.1). The factors associated with SP when we study all workers are age, seniority, salary structure, working more than 48 hours, the contribution of worker's wage to the total household income and downsizing; factors among the 'unhealthy' workers are working more than 48 hours and not having a contract. The most common reason for SP is 'did not want to burden my colleagues', 45.7% (95% CI 37.3 to 54.4), whereas 'I could not afford it for economic reasons' ranked third, 35.9% (29.4% to 42.9%), and 27.5% (21.3% to 34.6%) of the workers report 'worried about being laid off' as a reason for going to work despite being ill. CONCLUSIONS: The estimated frequency of SP in Spain is lower than certain other countries, such as the Scandinavian countries. The factors associated vary depending on the population analysed (all workers or excluding 'healthy' workers). The reason 'I was worried about being laid off' was much more common than the estimates for Sweden or Norway.

[Trends of HIV mother-to-child transmission in Catalonia, Spain, between 1987 and 2003]. / Evolución de la transmisión vertical del VIH en Cataluña durante el período 1987-2003.

BACKGROUND AND OBJECTIVE: To describe and to analyze the evolution of the mother-to-child transmission (MTCT) of the human immunodeficiency virus (HIV), the clinical and epidemiological characteristics and the use of antiretrovirals (ARV) in the HIV infected pregnant women and their new-borns alive between 1987 and 2003 in Catalonia. MATERIAL AND METHOD: The available clinical-epidemiological and treatment data were obtained from 4 reference hospitals that take care of most of the children born to HIV infected mothers in Catalonia. Two of the hospitals had a data base designed to the follow up of their patients, whereas in the other 2 data were gathered by reviewing clinical registries. For the analysis, 3 periods, based on the recommendations of treatment ARV during pregnancy, were settled down: 1987-1993; 1994-1996, and 1997-2003. RESULTS: 1,105 mother-infant pairs were studied. HIV MTCT was reduced from 20.4% to 3.5% from first to third period of study (p < 0.001). The median age of the mothers increased from 24.6 to 30.5 years of age (p < 0.001). The proportion of women infected by sexual transmission increased from 17.2% to 58.8% (p < 0.001), whereas that of parenteral transmission decreased from the 79.2% to 43.5% (p < 0.001). In the last period, 74.1% of mother-child pairs received complete ARV prophylaxis regimens and 21.6% partial ones. The rate of elective caesarean-section went up from 32.2% to 58.2% (p < 0.001). CONCLUSIONS: The rates of MTCT in our setting have followed the same trend as in other countries of our surroundings. The observed changes reflect the variations in the characteristics of the epidemic in the general population. The implementation of the recommendations on ARV prophylaxis has begun early and it has extended progressively without getting to be total. Additional strategies for the universal coverage of the screening test during pregnancy are still needed.

[Cooking Skills and Consumption of Ready Meal in University Students of Barcelona, Spain]. / Competencias culinarias y consumo de alimentos procesados o preparados en estudiantes universitarios de Barcelona.

OBJECTIVE: Confidence in cooking skills is relevant in health. The objective was to study the status of cooking skills and consumption of ready meal among university students in the city of Barcelona, assessing their association with sociodemographic variables and confidence in some culinary skills and knowing how to cook. METHODS: Cross-sectional study, carried out during the 2014-2015 academic year, 525 students (81.3% women, median age 21 years) using an on-line questionnaire. Bivariate and multivariate analyses were performed; odds ratios and 95% confidence intervals were estimated. RESULTS: Between 53% and 89% of the students reported feeling very confident in 10 of the 18 cooking skills. No statistically significant differences were observed by sex, and between 62% and 86% of those over 21 years expressed great confidence in 9 competitions. The consumption of ready meal was prevalent (49.4%) and associated with age (OR=0.95 95% CI: 0.91-0.99), gender (male, OR=1.98 95% CI: 1.23-3.18) and not knowing how to cook (OR=2.25 95% CI: 1.10-4.60). In students who knew how to cook, eating ready meal was associated with gender (male, OR =1.67 95% CI: 1.00 to 2.77) and no longer feel confident in preparing more than one food at one time (OR = 1.73 95% CI: 1.58 to 2.60). CONCLUSIONS: Students at a university in Barcelona have a level in cooking skills which could be improved, and consume ready meals usually.

OBJETIVO: Las competencias culinarias pueden tener relevancia en la salud. El objetivo del estudio fue conocer el estado de las competencias culinarias y el consumo de alimentos procesados o preparados en estudiantes universitarios en Barcelona así como su asociación con variables sociodemográficas y la confianza en algunas competencias culinarias y el saber cocinar. METODOS: Diseño transversal, durante el curso 2014-2015, con 525 estudiantes (81,3% mujeres, edad mediana 21 años), mediante un cuestionario en línea. Se realizaron análisis bivariados y multivariados con modelos de regresión logística. Como medida de asociación se estimó la odds ratio y su intervalo de confianza del 95%. RESULTADOS: Entre el 53% y el 89% de los estudiantes declararon sentirse muy confiados en 10 de las 18 competencias culinarias estudiadas. No se observaron diferencias estadísticamente significativas por sexo y entre el 62% y el 86% de las personas mayores de 21 años expresaron mucha confianza en 9 competencias. La prevalencia del consumo de alimentos procesados o preparados fue del 49,4% y se asoció a la edad (OR=0,95 IC95%: 0,91-0,99), al sexo (hombre, OR=1,98 IC95%: 1,23-3,18) y a no saber cocinar (OR=2,25 IC95%: 1,10-4,60). Entre quienes sabían cocinar este consumo se asoció al sexo (hombre, OR=1,67 IC95%: 1,00-2,77) y a no tener confianza en preparar más de un alimento al mismo tiempo (OR=1,73 IC95%: 1,58-2,60). CONCLUSIONES: Los estudiantes universitarios de Barcelona tienen competencias culinarias mejorables y consumen alimentos procesados o preparados habitualmente.

Stable long-term indigo production by overexpression of dioxygenase genes using a chromosomal integrated cascade expression circuit.

In our laboratory we have analyzed different factors to maximize the yield in heterologous protein expression for long-term cultivation, by combination of an efficient cascade expression system and stable integration in the bacterial chromosome. In this work, we have explored this system for the production of indigo dye as a model for biotechnological production, by expressing in Escherichia coli the thnA1A2A3A4 genes from Sphingomonas macrogolitabida strain TFA, which encode the components of a tetralin dioxygenase activity. We compared Ptac, and the Pm-based cascade expression circuit in a multicopy plasmid and stably integrated into the bacterial chromosome. Plasmid-based expression systems resulted in instability of indigo production when serially diluted batch experiments were performed without a selective pressure. This problem was solved by integrating the expression module in the chromosome. Despite the gene dosage reduction, the synergic effect of the cascade expression system produced comparable expression to the dioxygenase activity in the plasmid configuration but could be stably maintained for at least 5 days. Here, we show that the cascade amplification circuit integrated in the chromosome could be an excellent system for tight control and stable production of recombinant products.

[Estimating non work-related sickness leave absences related to a previous occupational injury in Catalonia (Spain)]. / Estimación de episodios de incapacidad temporal por contingencia común relacionados con un accidente de trabajo previo en Cataluña / Estimació d'episodis d'incapacitat temporal per contingències comunes relacionats amb un accident de treball previ a Catalunya.

OBJECTIVES: To estimate the frequency of non-work sickness absence (ITcc) related to previous occupational injuries with (ATB) or without (ATSB) sick leave. METHODS: Prospective longitudinal study. Workers with ATB or ATSB notified to the Occupational Accident Registry of Catalonia were selected in the last term of 2009. They were followed-up for six months after returning to work (ATB) or after the accident (ATSB), by sex and occupation. Official labor and health authority registries were used as information sources. An "injury-associated ITcc" was defined when the sick leave occurred in the following six months and within the same diagnosis group. The absolute and relative frequency were calculated according to time elapsed and its duration (cumulated days, measures of central trend and dispersion), by diagnosis group or affected body area, as compared to all of Catalonia. RESULTS: 2,9%of ATB (n=627) had an injury-associated ITcc, with differences by diagnosis, sex and occupation; this was also the case for 2,1% of ATSB (n=496).With the same diagnosis, duration of ITcc was longer among those who had an associated injury, and with respect to all of Catalonia. CONCLUSIONS: Some of the under-reporting of occupational pathology corresponds to episodes initially recognized as being work-related. Duration of sickness absence depends not only on diagnosis and clinical course, but also on criteria established by the entities managing the case. This could imply that more complicated injuries are referred to the national health system, resulting in personal, legal, healthcare and economic cost consequences for all involved stakeholders.

[Occupational exposure to carcinogens: analysis of the application of the CAREX information system to Catalonia]. / Exposición laboral a cancerígenos: análisis del sistema de información CAREX para su uso en Cataluña.

OBJECTIVE: The objective of this study is to estimate occupational exposures to human carcinogens in Catalonia in 2009, taking as a reference the CAREX ESP 2007 information system, and to evaluate the suitability of extrapolating these data to Catalonia. METHODS: The reference population is the number of people registered with the Social Security system in Catalonia in 2009. Carcinogens considered are those which the International Agency for Research on Cancer (IARC) classified into groups 1 and 2A and are related to occupational exposures. The exposure prevalences from the CAREX ESP 2007, adapted to the Catalonian Industrial Classification (CCAE 09), were used. Technical survey reports from the Occupational Safety and Health Centers of the Catalonian local government, and related databases were consulted. RESULTS: The most frequent occupational exposures to human carcinogens were solar radiation, crystalline silica, diesel exhaust, radon and wood dust, although based mainly on data not considered adequate for extrapolation to Catalonia. Around 217 exposure situations for 25 carcinogens, not previously considered in CAREX ESP 2007, were identified. CONCLUSIONS: The estimated number of workers exposed to human carcinogens in Catalonia in 2009 based on the CAREX ESP 2007 system could differ from the real situation. Development of a CAREX CAT system that incorporates exposure data from Catalonia is recommended.

OBJETIVO: El objetivo de este estudio es estimar la exposición laboral a cancerígenos en Cataluña para el año 2009 utilizando el sistema de información CAREX ESP 2007 como referencia, y valorar la adecuación o no de extrapolar estos datos a Cataluña. MÉTODOS: La población de referencia es aquella afiliada a cualquier régimen de la Seguridad Social de Cataluña en 2009. Se han considerado agentes cancerígenos los clasificados por la IARC (International Agency for Research on Cancer) en los grupos 1 y 2A relacionados con exposiciones laborales. Se ha utilizado la prevalencia de exposición de CAREX ESP 2007 adaptada a la clasificación económica Clasificación Catalana de Actividades Económicas (CCAE 09). Además, se han consultado actuaciones de técnicos de los Centres de Seguretat i Salut Laboral de la Generalitat de Catalunya, y bases de datos relacionadas con este tema. RESULTADOS: Las exposiciones laborales estimadas más comunes a agentes cancerígenos han sido para radiación solar, sílice cristalina, humos diesel, radón y polvo de madera, aunque con datos considerados en buena medida no extrapolables a Cataluña. Además, se han detectado 217 situaciones de exposición para 25 agentes cancerígenos que no se habían considerado en el sistema CAREX ESP 2007. CONCLUSIONES: Las estimaciones de trabajadores expuestos en Cataluña a agentes cancerígenos en 2009 en base al sistema CAREX ESP 2007 podrían diferir de la realidad. Se considera necesario desarrollar un sistema CAREX CAT con incorporación de datos de exposición propios de Cataluña.

[Validation of the Copenhagen Burnout Inventory to assess professional burnout in Spain].

BACKGROUND: The Copenhagen Burnout Inventory (CBI) is a public domain questionnaire measuring the degree of psychological fatigue experienced in three subdimensions of Burnout: personal (PB), work-related (WB), and client-related Burnout (CB). The study aimed to examine the acceptability, reliability and construct validity of the Spanish version of CBI. METHOD: The study population consisted of 479 workers of educational centers, social work centres, healthcare centres and workers within the industry sector. Data was collected in 2009 through a self-administered questionnaire including the three CBI scales, sixteen scales of psychosocial work environment (COPSOQ ISTAS21) and perceived general and mental health and vitality (SF-36). RESULTS: Response rate was 78.7%. The three scales have an inter-item correlation average between 0.42 and 0.60 and a corrected item-total correlation between 0,49 and 0,83. The internal consistency of the three scales had Cronbach's α values of 0.90 for PB, 0.83 for WB and 0.82 for CB. CONCLUSIONS: Burnout was related to both psychosocial work environment and wellbeing measures in the expected direction and intensity. The items of the three scales show good discrimination capacity, good consistency and homogeneity. The three CBI scales have an acceptable internal consistency reliability index, slightly higher in PB. The discrimination capacity of the scales is verified through the discrimination index and the different levels between occupations and activities. These results demonstrate that the Spanish version of the CBI is a reliable and valid instrument for measuring Burnout.

Biofilm formation studies in microtiter plate format.

Although Candida biofilms have been clearly identified as playing an increasingly important role in human disease, their biology and the reason for their poor susceptibility to antifungal agents remain largely unknown. Over recent years, various models have been developed in order to better characterize Candida biofilms. Here, we describe a number of rapid, inexpensive microtiter-format techniques and strategies which can be used for large-scale screening procedures aimed at identifying genes involved in Candida biofilm formation and/or potential antifungal agents with activity against pathogen cells growing under these conditions. The procedures could also be easily adapted for studying biofilm structures with a range of microscopy techniques.