Enhancement of Odontoblastic Differentiation of Stem Cells from Exfoliated Deciduous Tooth Using N-acetylcysteine-An In Vitro Study.

AIM AND OBJECTIVE: The study was conducted to evaluate the effects of N-acetylcysteine (NAC) on the propagation and differentiation of stem cells from human exfoliated deciduous teeth(SHED). MATERIALS AND METHODS: SHEDs were isolated by explant culture method and characterized for stem cell properties using flow cytometry method. MTT assay and Cell Counting Kit-8 (CCK-8) assay were used to examine the viability and proliferation of the SHEDs. The effects of NAC-induced osteo/odontoblastic differentiation of SHEDs were determined by functional staining for mineralization, and the gene expression of osteo/odontoblastic transcription factors and proteins was evaluated by real-time quantitative reverse transcription-polymerase chain reaction(qRT-PCR) analyses. Protein levels of collagen type 1 (COL1), dentin sialophosphoprotein (DSPP), and dentin matrix acidic phosphoprotein 1(DMP-1) were calculated by the Western blot method to assess the osteo/odontogenic differentiation. RESULTS: SHEDs presented mesenchymal stem cell (MSC)-like characteristics on flow cytometric analysis. The cell viability and metabolic activity of SHEDs were increased with an increase in the concentrations of NAC from 0.5 to 10 nM. However, the concentrations of NAC from 0.5 to 2.5 mM did not affect cell proliferation. NAC incorporated at a concentration of 2.5 mM showed higher mineralization and considerably increased gene expression levels of runt-related transcription factor 2 (RUNX2), COL1A1, DSPP, and DMP-1. It significantly increased the protein expression of odontoblast-related matrix proteins like COL1, DSPP, and DMP-1. CONCLUSION: NAC regulates the healthy propagation of dental stem cells in vitro. Its effects on the differentiation of dental pulp SHEDs remain unidentified. This study explores that NAC can encourage the mineralization of SHEDs and differentiate them into the odontoblastic lineage. CLINICAL SIGNIFICANCE: The results propose that NAC could have a significant pharmacological role in activating and enhancing odontogenic differentiation of dental stem cells and possibly a prospect in regenerative dentistry.

Effect of Implant Instrumentation Techniques in the Success of Implants: An Original Research.

Background: The success rates of dental implants are largely dependent on implant instrumentation procedures. It's still unknown, nevertheless, how successful sophisticated instrumentation techniques are in comparison to older methods. Methods: One hundred patients receiving dental implants participated in a prospective study that was carried out in a tertiary care facility. Random assignments were made to place patients in Group B (advanced instrumentation) or Group A (conventional instrumentation). Following implant implantation, clinical indicators, such as implant stability and peri-implant bone loss, were evaluated on a regular basis. A statistical analysis was conducted in order to compare the results among the groups. Results: Compared to implants placed using conventional procedures (75.2 ± 3.4), implants placed using advanced instrumentation techniques showed considerably improved stability (82.6 ± 2.9) (P < 0.001). Furthermore, there was a statistically significant difference in peri-implant bone loss between the advanced instrumentation group (0.4 ± 0.1 mm) and the traditional instrumentation group (0.8 ± 0.2 mm) (P = 0.003). Conclusion: In summary, current research indicates that, in comparison to conventional methods, sophisticated instrumentation techniques enhance implant stability and decrease peri-implant bone loss. These findings have significant ramifications for implant dentistry clinical procedures as well as patient outcomes.