Development of 18 microsatellite loci for the freshwater snail Bellamya aeruginosa (Mollusca, Gastropoda).

Although it is a major freshwater gastropod species, genetic diversity of Bellamya aeruginosa was completely unknown. Eighteen microsatellite loci were isolated and characterized from (AC)(15)-enriched genomic libraries of the freshwater snail B. aeruginosa. Most of the 18 loci were successfully amplified and high polymorphic information content values were found, ranging from 0.244 to 0.792 (mean 0.541). The number of alleles per locus ranged from 5 to 13 (mean 8.8), the expected heterozygosity varied from 0.347 to 0.950 (mean 0.815) and the observed heterozygosity varied from 0.087 to 0.782 (mean 0.431). Eight loci showed significant deviation from Hardy-Weinberg equilibrium after Bonferroni's correction and no significant genotypic linkage disequilibrium was detected between most locus pairs, except for TXH79-TXH97 and TXH113-TXH121. These 18 polymorphic microsatellite loci should be useful for population genetics analysis and species identification of Bellamya.

[Clinical and experimental study of No. 90-dasheng jiangya oral liquid in treating hypertension].

UNLABELLED: 109 cases of hypertension patients were divided into two groups at random. No. 90 Dasheng Jiangya (DSJY) oral liquid was administered to the TCM group, while nifedipine to the control. RESULTS: The level of hypertension was lowered in both TCM and control groups. The effective rates of which were 92.3% and 94.7% respectively. No significant difference was seen between them (P > 0.05). The TCM groups has an advantage of decreasing blood-lipid, increasing high density lipoprotein, improving microcirculation and reducing the atherogenic index as well. The difference between the two groups was significant (P < 0.05 or 0.01). Animal studies have shown that TCM groups has effect in decreasing blood pressure of the spontaneously hypertensive rat, and in rising of anoxia tolerance in mice. Both clinical and toxicological tests showed that this drug was non-toxic with no marked side-effects. Therefore this paper provides a basis for use of No. 90 Dasheng Jiangya oral liquid in preventing and treating hypertension.