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1.
Am J Respir Crit Care Med ; 190(11): 1263-72, 2014 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-25333685

RESUMO

RATIONALE: Idiopathic pulmonary fibrosis (IPF) is an untreatable and often fatal lung disease that is increasing in prevalence and is caused by complex interactions between genetic and environmental factors. Epigenetic mechanisms control gene expression and are likely to regulate the IPF transcriptome. OBJECTIVES: To identify methylation marks that modify gene expression in IPF lung. METHODS: We assessed DNA methylation (comprehensive high-throughput arrays for relative methylation arrays [CHARM]) and gene expression (Agilent gene expression arrays) in 94 patients with IPF and 67 control subjects, and performed integrative genomic analyses to define methylation-gene expression relationships in IPF lung. We validated methylation changes by a targeted analysis (Epityper), and performed functional validation of one of the genes identified by our analysis. MEASUREMENTS AND MAIN RESULTS: We identified 2,130 differentially methylated regions (DMRs; <5% false discovery rate), of which 738 are associated with significant changes in gene expression and enriched for expected inverse relationship between methylation and expression (P < 2.2 × 10(-16)). We validated 13/15 DMRs by targeted analysis of methylation. Methylation-expression quantitative trait loci (methyl-eQTL) identified methylation marks that control cis and trans gene expression, with an enrichment for cis relationships (P < 2.2 × 10(-16)). We found five trans methyl-eQTLs where a methylation change at a single DMR is associated with transcriptional changes in a substantial number of genes; four of these DMRs are near transcription factors (castor zinc finger 1 [CASZ1], FOXC1, MXD4, and ZDHHC4). We studied the in vitro effects of change in CASZ1 expression and validated its role in regulation of target genes in the methyl-eQTL. CONCLUSIONS: These results suggest that DNA methylation may be involved in the pathogenesis of IPF.


Assuntos
Metilação de DNA/genética , Epigênese Genética/fisiologia , Fibrose Pulmonar Idiopática/genética , Locos de Características Quantitativas/genética , Transcriptoma/genética , Corticosteroides/uso terapêutico , Estudos de Casos e Controles , Feminino , Expressão Gênica , Marcadores Genéticos , Humanos , Imunossupressores/uso terapêutico , Masculino , Pessoa de Meia-Idade , Fumar/epidemiologia
2.
Vaccine ; 22(27-28): 3535-45, 2004 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-15315833

RESUMO

Recent efforts to design an human immunodeficiency virus type 1 (HIV-1) vaccine candidate have focused on means of eliciting anti-viral T-cell responses. We tried to improve the immunogenicity of DNA vaccines by designing hybrid DNA constructs encoding hepatitis B surface antigen (HBsAg) fused to antigenic domains of simian/human immunodeficiency virus (SHIV 89.6P). Immunisation with hybrid DNA induced both effector and long-lasting precursor T-cells. Following boosting with a recombinant modified vaccinia Ankara (rMVA) producing full-length SIV and HIV antigens, it appeared that priming with hybrid DNA had increased virus-specific T-cell responses in terms of both the number of virus-specific IFN-gamma-secreting T-cells and virus-specific lymphoproliferation. After intrarectal challenge with SHIV 89.6P, immunised animals demonstrated early control of SHIV 89.6P replication and stable CD4+ T-cell counts.


Assuntos
Vacinas contra a AIDS/uso terapêutico , DNA/genética , DNA/imunologia , HIV/imunologia , Antígenos de Superfície da Hepatite B/imunologia , Vacinas contra a SAIDS/uso terapêutico , Vírus da Imunodeficiência Símia/imunologia , Linfócitos T/imunologia , Animais , Área Sob a Curva , Linfócitos T CD4-Positivos/imunologia , Divisão Celular/efeitos dos fármacos , Citomegalovirus/genética , Sondas de DNA , Ensaio de Imunoadsorção Enzimática , Vetores Genéticos , Antígenos de Superfície da Hepatite B/genética , Imunização Secundária , Interferon gama , Cinética , Macaca mulatta , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Linfócitos T/efeitos dos fármacos , Vacinas de DNA/uso terapêutico , Vacinas de Subunidades Antigênicas/uso terapêutico , Vacínia/imunologia , Replicação Viral
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