Viral predation pressure on coral reefs.

BACKGROUND: Predation pressure and herbivory exert cascading effects on coral reef health and stability. However, the extent of these cascading effects can vary considerably across space and time. This variability is likely a result of the complex interactions between coral reefs' biotic and abiotic dimensions. A major biological component that has been poorly integrated into the reefs' trophic studies is the microbial community, despite its role in coral death and bleaching susceptibility. Viruses that infect bacteria can control microbial densities and may positively affect coral health by controlling microbialization. We hypothesize that viral predation of bacteria has analogous effects to the top-down pressure of macroorganisms on the trophic structure and reef health. RESULTS: Here, we investigated the relationships between live coral cover and viruses, bacteria, benthic algae, fish biomass, and water chemistry in 110 reefs spanning inhabited and uninhabited islands and atolls across the Pacific Ocean. Statistical learning showed that the abundance of turf algae, viruses, and bacteria, in that order, were the variables best predicting the variance in coral cover. While fish biomass was not a strong predictor of coral cover, the relationship between fish and corals became apparent when analyzed in the context of viral predation: high coral cover (> 50%) occurred on reefs with a combination of high predator fish biomass (sum of sharks and piscivores > 200 g m-2) and high virus-to-bacteria ratios (> 10), an indicator of viral predation pressure. However, these relationships were non-linear, with reefs at the higher and lower ends of the coral cover continuum displaying a narrow combination of abiotic and biotic variables, while reefs at intermediate coral cover showed a wider range of parameter combinations. CONCLUSIONS: The results presented here support the hypothesis that viral predation of bacteria is associated with high coral cover and, thus, coral health and stability. We propose that combined predation pressures from fishes and viruses control energy fluxes, inhibiting the detrimental accumulation of ecosystem energy in the microbial food web.

Spatiotemporal changes in largemouth bass mercury concentrations from Connecticut waterbodies, 1995-2021.

We evaluated spatiotemporal changes in the mean and variation in largemouth bass (Micropterus salmoides) mercury concentrations over three discrete time periods (1995, 2005-2006, and 2019-2021) across 56 Connecticut waterbodies. We detected largemouth bass raw mercury concentrations that exceeded the US Environmental Protection Agency (USEPA) Fish Tissue Residue Criterion (≥ 0.30 µg g-1 ww) in 75.1%, 63.3%, and 47.7% of all fish sampled during 1995, 2005-2006, and 2019-2021, respectively. Total length (TL)-adjusted largemouth bass mercury concentrations declined across all ecoregions in Connecticut between subsequent sampling periods but increased between 2005-2006 and 2019-2021 in the Northwest Hills/Uplands ecoregion. The coefficient of variation (CV) of largemouth bass TL-adjusted mercury concentrations increased through time, increasing from 25.78% during 1995 to 36.47% during 2019-2021. The probability of a largemouth bass having a raw mercury concentration > 0.30 µg g-1 ww increased with total length (TL), but the TL with a 50% probability varied across ecoregions and periods. The variation in largemouth bass mercury concentrations highlights the roles that changes to individual behaviors, food web structure, lake properties, and legacy mercury may play in shaping broad patterns and trends in mercury consumption risks.

Human Trafficking Cases Presenting within Child Advocacy Centers.

Although human trafficking of minors is an increasing concern within the United States, very little information is known about how trafficking cases are processed within child advocacy centers (CACs). The current study addresses this gap in the literature by providing descriptive information about victims, service referrals, and prosecutorial outcomes for human trafficking cases presenting at CACs across a Midwestern state. The data originates from a state-wide study focused on understanding the scope of human trafficking cases. Specifically, the dataset includes 210 youth presenting at CACs over a three-year period of time. In this sample, the typical human trafficking case involved sex trafficking of a self-identified white female victim, with an offender known to the victim. Most child survivors passing through CACs were referred to medical and mental health services, although these service referrals did not greatly differ across at-risk versus substantiated trafficking cases. Overall, the findings suggest that CACs are uniquely positioned to encounter human trafficking cases and provide needed services to trafficking survivors. Finally, recommendations are provided for CACs regarding the intake and identification of trafficking cases more broadly.

Estimating the Prevalence of Human Trafficking in Ohio, 2014-2016.

Objectives. To develop statewide estimates of known victims and individuals at risk of human trafficking in Ohio.Methods. We collected information from 12 state and local sources including child welfare, legal services, and law enforcement data. We collected the data from agency records dating 2013 to 2018. However, the majority of data were from calendar years 2014, 2015, and 2016 (roughly 95% across individual and aggregate sources). We used probabilistic matching to estimate victim and at-risk cases-accounting for duplicates.Results. According to available data, there were 1032 known victims during the study time frame. We identified approximately 4209 at-risk individuals based on youths presenting with common risk factors for trafficking victimization.Conclusions. Estimating the prevalence of human trafficking is an important public health research priority. As the first "cataloging" of existing record systems in Ohio to our knowledge, this study provided a comprehensive overview of the number of victims and the type of information that is available in the state. This study highlights the importance of moving toward the use of epidemiological approaches to measure the prevalence of human trafficking.

Resilience and vulnerability in adolescents: genetic influences on differential response to risk for delinquency.

Prior research has identified a vast number of correlates for delinquent behavior during adolescence, yet a considerable number of errors in prediction remain. These errors suggest that behavioral development among a portion of youths is not well understood, with some exhibiting resilience and others a heightened vulnerability to risks. Examining cases that do not confirm prediction outcomes provides an opportunity to achieve a greater understanding of the relationships between risk factors and delinquency, which can be used to improve theoretical explanations of behavior. This study explores the contribution of genetic and environmental factors to differences in individual responses to cumulative risk for delinquency among a sample of adolescent twins (N = 784 pairs, 49 % female) in the National Longitudinal Study of Adolescent Health. The results indicate that additive genetic and unique environmental factors significantly contribute to variation in responses to cumulative risk across 14 risk factors spanning individual, familial, and environmental domains. When analyzed separately, the majority of the difference between vulnerable youths and the overall population was attributed to genetic influences, while differences between resilient youths and the population were primarily attributed to environmental influences. The findings illustrate the importance of examining both genetic and environmental influences in order to enhance explanations of adolescent offending.

Peer influence and context: the interdependence of friendship groups, schoolmates and network density in predicting substance use.

This article focuses on the degree to which friends' influence on substance use is conditioned by the consistency between their behavior and that of schoolmates (individuals enrolled in the same school, but not identified as friends), contributing to the literature on the complexity of interactive social influences during adolescence. Specifically, it hypothesizes that friends' influence will diminish as their norms become less similar to that of schoolmates. The authors also propose that this conditioning relationship is related to the density of the friendship group. This study uses data from the National Longitudinal Survey of Adolescent Health (AddHealth) (n ~ 8,000, 55% female) to examine the interactive relationship between friend and schoolmate influences on adolescent substance use (smoking and drinking). The sample contains students ranging from age 11 to 22 and is 60% White. The findings demonstrate that, as the substance use of the friendship group becomes more dissimilar from schoolmates' substance use, the friendship group's influence on adolescent substance use diminishes. Further, the results demonstrate that this conditioning relationship does not emerge when the friendship group is highly dense.

Camera traps reveal that terrestrial predators are pervasive at riverscape cold-water thermal refuges.

Perceived predation risks by terrestrial predators are major ecological forces in aquatic systems, particularly for aggregating fish. Riverscape thermal refuges are discrete, localized cold-water patches where fish temporarily aggregate to buffer against heat events. Predation pressures by terrestrial predators at thermal refuges may decrease the thermoregulatory benefits of refuge use, but quantifying such effects can be challenging and controversial when sampling can impose additional stress on fish. We passively monitored terrestrial predator visitation patterns and predation at four thermal refuges in the Housatonic River, Connecticut, USA, between May 18th and September 29th, 2022, with camera traps, a common wildlife monitoring method. Specifically, we (1) assessed diel visitation patterns by different categories of terrestrial predators at thermal refuges and determined if patterns varied among predator categories or with prevailing environmental conditions, and (2) estimated the probability of predation by hour of the day combined across all predator categories, quantifying general predation pressures at refuges. We detected at least one terrestrial predator at a thermal refuge each day, and mean hourly visitation rates (count/h) were highly variable across predator categories and sampling dates. The most supported generalized additive mixed model indicated that terrestrial predator visitation rates (count/h/day) varied with mean daily river discharge and water temperature differential, and relationships differed across categories of terrestrial predators. We observed 22 separate predation attempts on thermoregulating salmonids and predicted that the probability of predation by any terrestrial predator increased from 0.002 to 0.017 throughout a 24 h day (p = .004). Camera traps provided novel evidence that terrestrial predators are pervasive at riverine thermal refuges, which is relevant for refuge conservation and management globally. We recommend the implementation of a coordinated monitoring network across riverine thermal refuges using camera traps, further enriching our ecological understanding of cumulative predator effects in refuges across complex riverscapes.

Microvascular repair: post-angiogenesis vascular dynamics.

Vascular compromise and the accompanying perfusion deficits cause or complicate a large array of disease conditions and treatment failures. This has prompted the exploration of therapeutic strategies to repair or regenerate vasculatures, thereby establishing more competent microcirculatory beds. Growing evidence indicates that an increase in vessel numbers within a tissue does not necessarily promote an increase in tissue perfusion. Effective regeneration of a microcirculation entails the integration of new stable microvessel segments into the network via neovascularization. Beginning with angiogenesis, neovascularization entails an integrated series of vascular activities leading to the formation of a new mature microcirculation, and includes vascular guidance and inosculation, vessel maturation, pruning, AV specification, network patterning, structural adaptation, intussusception, and microvascular stabilization. While the generation of new vessel segments is necessary to expand a network, without the concomitant neovessel remodeling and adaptation processes intrinsic to microvascular network formation, these additional vessel segments give rise to a dysfunctional microcirculation. While many of the mechanisms regulating angiogenesis have been detailed, a thorough understanding of the mechanisms driving post-angiogenesis activities specific to neovascularization has yet to be fully realized, but is necessary to develop effective therapeutic strategies for repairing compromised microcirculations as a means to treat disease.

Vascular effects of a low-carbohydrate high-protein diet.

The cardiovascular complications of obesity have prompted interest in dietary interventions to reduce weight, including low-carbohydrate diets that are generally high in protein and fat. However, little is known about the long-term effects of these diets on vascular health. We examined the cardiovascular effects of a low-carbohydrate, high-protein diet (LCHP) in the ApoE(-/-) mouse model of atherosclerosis and in a model of ischemia-induced neovascularization. Mice on a LCHP were compared with mice maintained on either the standard chow diet (SC) or the Western diet (WD) which contains comparable fat and cholesterol to the LCHP. LCHP-fed mice developed more aortic atherosclerosis and had an impaired ability to generate new vessels in response to tissue ischemia. These changes were not explained by alterations in serum cholesterol, inflammatory mediators or infiltrates, or oxidative stress. The LCHP diet substantially reduced the number of bone marrow and peripheral blood endothelial progenitor cells (EPCs), a marker of vascular regenerative capacity. EPCs from mice on a LCHP diet also manifest lower levels of activated (phosphorylated) Akt, a serine-threonine kinase important in EPC mobilization, proliferation, and survival. Taken together, these data demonstrate that in animal models LCHP diets have adverse vascular effects not reflected in serum markers and that nonlipid macronutrients can modulate vascular progenitor cells and pathophysiology.

Exploring Transfers between Earth-Moon Halo Orbits via Multi-Objective Reinforcement Learning.

Multi-Reward Proximal Policy Optimization, a multi-objective deep reinforcement learning algorithm, is used to examine the design space of low-thrust trajectories for a SmallSat transferring between two libration point orbits in the Earth-Moon system. Using Multi-Reward Proximal Policy Optimization, multiple policies are simultaneously and efficiently trained on three distinct trajectory design scenarios. Each policy is trained to create a unique control scheme based on the trajectory design scenario and assigned reward function. Each reward function is defined using a set of objectives that are scaled via a unique combination of weights to balance guiding the spacecraft to the target mission orbit, incentivizing faster flight times, and penalizing propellant mass usage. Then, the policies are evaluated on the same set of perturbed initial conditions in each scenario to generate the propellant mass usage, flight time, and state discontinuities from a reference trajectory for each control scheme. The resulting low-thrust trajectories are used to examine a subset of the multi-objective trade space for the SmallSat trajectory design scenario. By autonomously constructing the solution space, insights into the required propellant mass, flight time, and transfer geometry are rapidly achieved.

When early crime prevention goes to scale: a new look at the evidence.

It is a widely held view--in both research and policy communities--that desirable effects on delinquency and later offending from early prevention trials will attenuate once they are "scaled-up" or "rolled-out" for wider public use. Some of the main reasons for this include a reduced level of risk, a more heterogeneous population, insufficient service infrastructure, and loss of program fidelity. If attenuation of program effects is not only possible but is highly probable, then the issue for researchers and policymakers should be how to preserve or even enhance effects in moving from efficacy trials to community effectiveness trials to broad-scale dissemination. This paper surveys the knowledge base in an effort to contribute to an improved understanding of the theoretical and empirical dimensions for successfully taking early crime prevention programs to scale. It also outlines some proposals for how future research can make progress on this critical policy issue.

Adolescent risk behavior subgroups: an empirical assessment.

Theories and prior research have outlined a constellation of adolescent risk behaviors that tend to co-occur, reflecting a general pattern. Although their generality has largely been supported, there is some question about how to best study and portray the relationship among these behaviors. This study used data from a survey administered to high school youth (n = 2549, 38 schools). The general population sample comprised an even split between boys and girls, averaged roughly 16 years of age, and was 59% White and 10% Hispanic/Latino. Using latent class analysis, four subgroups, comprised of varying types and degrees of risky behavior, were identified. Specifically, there were two groups that "abstained" and "experimented" with risky behaviors and two others that had higher, but somewhat distinct, patterns of such activities. These groups were then examined in relation to youth characteristics (e.g., mental and physical health, school performance) and socio-environmental factors (e.g., social support, parental monitoring) that may be useful for better understanding "problem behavior syndrome" and development of prevention strategy.

Considering the rates of growth in two taxa of coral across Pacific islands.

Reef-building coral taxa demonstrate considerable flexibility and diversity in reproduction and growth mechanisms. Corals take advantage of this flexibility to increase or decrease size through clonal expansion and loss of live tissue area (i.e. via reproduction and mortality of constituent polyps). The biological lability of reef-building corals may be expected to map onto varying patterns of demography across environmental contexts which can contribute to geographic variation in population dynamics. Here we explore the patterns of growth of two common coral taxa, corymbose Pocillopora and massive Porites, across seven islands in the central and south Pacific. The islands span a natural gradient of environmental conditions, including a range of pelagic primary production, a metric linked to the relative availability of inorganic nutrients and heterotrophic resources for mixotrophic corals, and sea surface temperature and thermal histories. Over a multi-year sampling interval, most coral colonies experienced positive growth (greater planar area of live tissue in second relative to first time point), though the distributions of growth varied across islands. Island-level median growth did not relate simply to estimated pelagic primary productivity or temperature. However, at locations that experienced an extreme warm-water event during the sampling interval, most Porites colonies experienced net losses of live tissue and nearly all Pocillopora colonies experienced complete mortality. While descriptive statistics of demographics offer valuable insights into trends and variability in colony change through time, simplified models predicting growth patterns based on summarized oceanographic metrics appear inadequate for robust demographic prediction. We propose that the complexity of life history strategies among colonial reef-building corals introduces unique demographic flexibility for colonies to respond to a wide breadth of environmental conditions.

A model of vulnerability for adult sexual victimization: the impact of attachment, child maltreatment, and scarred sexuality.

Extending previous research, this study utilized structural equation modeling to examine the effects of poor mother/child attachment, child neglect, juvenile sexual victimization (JSV), and Finkelhor and Browne's (1985) proposed construct of traumatic sexualization on vulnerability to adult sexual victimization. The proposed model was assessed using data drawn from a sample of African American females involved in a prospective study of child sexual abuse survivors. This group was matched to similar others without such history. Findings suggest that child neglect worsens with poor mother/child attachment, resulting in a greater likelihood of JSV. Both neglect and JSV impact shaming sexual beliefs and behaviors, contributing to the risk for adult sexual victimization. This set of variables accounted for 27% of variance in adult sexual victimization.

The Short-Term Dynamics of Peers and Delinquent Behavior: An Analysis of Bi-weekly Changes Within a High School Student Network.

OBJECTIVES: To analyze short-term changes in peer affiliations, offending behavior and routine activities in order to evaluate three different processes: peer selection, peer socialization and situational peer influences. METHODS: The short-term longitudinal TEENS study was conducted among a cohort of students from one mid-sized high school in Kentucky, as part of the larger Rural Substance Abuse and Violence Project. The study sample consists of one complete network of 155 ninth graders who completed surveys about their peer affiliations, routine activities and offending behaviors over the course of five waves of data collection during the beginning of the school year. The measurement intervals were no more than 2 weeks long. Longitudinal network analysis (SIENA software that enables actor-oriented stochastic modeling) was used to estimate peer selection, socialization, and situational effects. RESULTS: Peer networks, offending, and routine activities appeared to be very volatile over the research period. Peer selection effects were found for structural network properties, demographics and delinquent values, but not for peer delinquency. We did not find significant peer socialization effects within the research period, but instead found that changes in offending were related to situational changes in unstructured socializing, alcohol use and marijuana use. CONCLUSIONS: The results suggest that traditional time lags of one year or six months between measurements may fail to capture short-term relations between peers and behavior. Long-term peer influence processes like socialization may be less important in the short run, while situational peer effects might be more salient.

Differential Regulation of Bladder Pain and Voiding Function by Sensory Afferent Populations Revealed by Selective Optogenetic Activation.

Bladder-innervating primary sensory neurons mediate reflex-driven bladder function under normal conditions, and contribute to debilitating bladder pain and/or overactivity in pathological states. The goal of this study was to examine the respective roles of defined subtypes of afferent neurons in bladder sensation and function in vivo via direct optogenetic activation. To accomplish this goal, we generated transgenic lines that express a Channelrhodopsin-2-eYFP fusion protein (ChR2-eYFP) in two distinct populations of sensory neurons: TRPV1-lineage neurons (Trpv1Cre;Ai32, the majority of nociceptors) and Nav1.8+ neurons (Scn10aCre;Ai32, nociceptors and some mechanosensitive fibers). In spinal cord, eYFP+ fibers in Trpv1Cre;Ai32 mice were observed predominantly in dorsal horn (DH) laminae I-II, while in Scn10aCre;Ai32 mice they extended throughout the DH, including a dense projection to lamina X. Fiber density correlated with number of retrogradely-labeled eYFP+ dorsal root ganglion neurons (82.2% Scn10aCre;Ai32 vs. 62% Trpv1Cre;Ai32) and degree of DH excitatory synaptic transmission. Photostimulation of peripheral afferent terminals significantly increased visceromotor responses to noxious bladder distension (30-50 mmHg) in both transgenic lines, and to non-noxious distension (20 mmHg) in Scn10aCre;Ai32 mice. Depolarization of ChR2+ afferents in Scn10aCre;Ai32 mice produced low- and high-amplitude bladder contractions respectively in 53% and 27% of stimulation trials, and frequency of high-amplitude contractions increased to 60% after engagement of low threshold (LT) mechanoreceptors by bladder filling. In Trpv1Cre;Ai32 mice, low-amplitude contractions occurred in 27% of trials before bladder filling, which was pre-requisite for light-evoked high-amplitude contractions (observed in 53.3% of trials). Potential explanations for these observations include physiological differences in the thresholds of stimulated fibers and their connectivity to spinal circuits.

Reducing out-of-community placement and recidivism: diversion of delinquent youth with mental health and substance use problems from the justice system.

For a number of reasons, researchers and policy makers are now focusing on diversion of youth from the juvenile justice system. This study examines New York State's diversion efforts focused on youth with mental health and substance abuse problems who are at risk of out-of-community placement (N = 2,309). Twelve counties participated in the Mental Health Juvenile Justice (MH/JJ) Diversion Project. Overall, the initiative was successful in reducing out-of-community placement and recidivism for diverted youth. In regression analysis site, services received, and problem-profile variables were important predictors of out-of-community placement. Prior record, prior placement, and significant mental health problems were statistically significant predictors of recidivism. This suggests some contrasts in explaining the results of two important goals of the MH/JJ project. Study results and implications for future research and intervention are discussed.

Continuity and Change in Adolescent Victimization: Examining the Effects of Stable and Dynamic Influences.

After decades of treatment as a fairly distinct topic, recent research on victimization has begun to draw on theoretical approaches previously directed at understanding criminal behavior. The current study expands this research by studying victimization and its relationship to key developmental influences with data from 3,976 adolescents. We first detail the longitudinal process that underlies continuity and change in victimization and then consider the impact of time-stable and time-varying covariates that reflect mechanisms within those explanations. Findings suggest that time-varying markers of risky lifestyle and attachment affect victimization, but also that victimization affects risky behaviors and prosocial ties.

Similar Mechanisms? A Comparative Longitudinal Study of Adolescent Violence and Victimization.

This study analyzes the extent to which there are similar patterns of violent offending and victimization in a panel of adolescents. Three explanatory perspectives are assessed: population heterogeneity, state dependence, and a mixed model. Data are drawn from a four-wave panel study of 3,976 adolescents. The main study measures comprise self-report indices for victimization and delinquency. Theoretical perspectives are specified through three distinct statistical approaches--latent growth curve, autoregressive simplex, and autoregressive latent trajectory (ALT) models. The analysis then incorporates the effects of relevant time-stable and time-varying influences. A mixed perspective, represented by the ALT model, best fits the data for both violent victimization and offending. Covariates drawn from the two perspectives have similar effects as well. The findings provide some support for a similar mechanisms hypothesis.

A design and focus group evaluation of dietary choices tools for an underserved population.

INTRODUCTION: Our objective was to develop and evaluate dietary teaching tools for a select population diagnosed with a severe mental illness and limited financial ability. Patients with severe mental illnesses face many challenges, including common health comorbidities of diabetes, high blood pressure, high cholesterol, and obesity. Cognitive deficits may limit educational programming; financial resources can affect access to a healthy diet. The Integrated Multidisciplinary Program of Assertive Community Treatment (IMPACT) program, a university-based program, provides individualized services to this population. One focus is healthy nutritional choices. METHODS: In Phase One, a clinical pharmacist and a first-year pharmacy resident created visual aids. These cards were given to health care providers (HCPs) to be used with IMPACT members. HCPs were asked to participate in a focus group and provide feedback. Phase Two: Based on specific focus group feedback, additional resources were created to address identified nutritional needs. RESULTS: Phase One: Ten cards were created and distributed to the HCPs. A focus group was conducted. HCPs reported the cards were useful in opening dietary choices dialogues and were able to give more specific information on alternative choices. Phase Two: From focus group feedback, specific cards for disease states, calorie guidelines, and budget limitations were developed. HCPs immediately utilized them. DISCUSSION: This pilot project was used to design and create educational cards to facilitate discussions on healthy or healthier dietary choices. Feedback from the HCPs participating in the focus group was positive, and they were enthusiastic about both sets of cards, particularly those pertaining to budget choices.