RESUMO
As the capability of mass spectrometry-based proteomics has matured, tens of thousands of peptides can be measured simultaneously, which has the benefit of offering a systems view of protein expression. However, a major challenge is that, with an increase in throughput, protein quantification estimation from the native measured peptides has become a computational task. A limitation to existing computationally driven protein quantification methods is that most ignore protein variation, such as alternate splicing of the RNA transcript and post-translational modifications or other possible proteoforms, which will affect a significant fraction of the proteome. The consequence of this assumption is that statistical inference at the protein level, and consequently downstream analyses, such as network and pathway modeling, have only limited power for biomarker discovery. Here, we describe a Bayesian Proteoform Quantification model (BP-Quant)(1) that uses statistically derived peptides signatures to identify peptides that are outside the dominant pattern or the existence of multiple overexpressed patterns to improve relative protein abundance estimates. It is a research-driven approach that utilizes the objectives of the experiment, defined in the context of a standard statistical hypothesis, to identify a set of peptides exhibiting similar statistical behavior relating to a protein. This approach infers that changes in relative protein abundance can be used as a surrogate for changes in function, without necessarily taking into account the effect of differential post-translational modifications, processing, or splicing in altering protein function. We verify the approach using a dilution study from mouse plasma samples and demonstrate that BP-Quant achieves similar accuracy as the current state-of-the-art methods at proteoform identification with significantly better specificity. BP-Quant is available as a MatLab® and R packages.
Assuntos
Proteínas Sanguíneas/análise , Processamento de Proteína Pós-Traducional , Proteoma/análise , Proteômica/estatística & dados numéricos , Software , Processamento Alternativo , Sequência de Aminoácidos , Animais , Teorema de Bayes , Proteínas Sanguíneas/genética , Proteínas Sanguíneas/metabolismo , Humanos , Camundongos , Dados de Sequência Molecular , Proteoma/genética , Proteoma/metabolismo , Proteômica/métodosRESUMO
As the capability of mass spectrometry-based proteomics has matured, tens of thousands of peptides can be measured simultaneously, which has the benefit of offering a systems view of protein expression. However, a major challenge is that with an increase in throughput, protein quantification estimation from the native measured peptides has become a computational task. A limitation to existing computationally-driven protein quantification methods is that most ignore protein variation, such as alternate splicing of the RNA transcript and post-translational modifications or other possible proteoforms, which will affect a significant fraction of the proteome. The consequence of this assumption is that statistical inference at the protein level, and consequently downstream analyses, such as network and pathway modeling, have only limited power for biomarker discovery. Here, we describe a Bayesian model (BP-Quant) that uses statistically derived peptides signatures to identify peptides that are outside the dominant pattern, or the existence of multiple over-expressed patterns to improve relative protein abundance estimates. It is a research-driven approach that utilizes the objectives of the experiment, defined in the context of a standard statistical hypothesis, to identify a set of peptides exhibiting similar statistical behavior relating to a protein. This approach infers that changes in relative protein abundance can be used as a surrogate for changes in function, without necessarily taking into account the effect of differential post-translational modifications, processing, or splicing in altering protein function. We verify the approach using a dilution study from mouse plasma samples and demonstrate that BP-Quant achieves similar accuracy as the current state-of-the-art methods at proteoform identification with significantly better specificity. BP-Quant is available as a MatLab ® and R packages at https://github.com/PNNL-Comp-Mass-Spec/BP-Quant.
RESUMO
Ensuring data quality and proper instrument functionality is a prerequisite for scientific investigation. Manual quality assurance is time-consuming and subjective. Metrics for describing liquid chromatography mass spectrometry (LC-MS) data have been developed; however, the wide variety of LC-MS instruments and configurations precludes applying a simple cutoff. Using 1150 manually classified quality control (QC) data sets, we trained logistic regression classification models to predict whether a data set is in or out of control. Model parameters were optimized by minimizing a loss function that accounts for the trade-off between false positive and false negative errors. The classifier models detected bad data sets with high sensitivity while maintaining high specificity. Moreover, the composite classifier was dramatically more specific than single metrics. Finally, we evaluated the performance of the classifier on a separate validation set where it performed comparably to the results for the testing/training data sets. By presenting the methods and software used to create the classifier, other groups can create a classifier for their specific QC regimen, which is highly variable lab-to-lab. In total, this manuscript presents 3400 LC-MS data sets for the same QC sample (whole cell lysate of Shewanella oneidensis), deposited to the ProteomeXchange with identifiers PXD000320-PXD000324.
Assuntos
Cromatografia Líquida/métodos , Cromatografia Líquida/normas , Espectrometria de Massas/métodos , Espectrometria de Massas/normas , Modelos Estatísticos , Controle de Qualidade , Reprodutibilidade dos Testes , Projetos de PesquisaRESUMO
This paper describes a new method for predicting the detectability of thin gaseous plumes in hyperspectral images. The novelty of this method is the use of basis vectors for each of the spectral channels of a collection instrument to calculate noise-equivalent concentration-pathlengths instead of matching scene pixels to absorbance spectra of gases in a library. This method provides insight into regions of the spectrum where gas detection will be relatively easier or harder, as influenced by ground emissivity, temperature contrast, and the atmosphere. Our results show that data collection planning could be influenced by information about when potential plumes are likely to be over background segments that are most conducive to detection.
Assuntos
Gases/análise , Modelos Químicos , Modelos Estatísticos , Análise Espectral/métodos , Processamento de Imagem Assistida por ComputadorRESUMO
Nanoparticles are potentially powerful therapeutic tools that have the capacity to target drug payloads and imaging agents. However, some nanoparticles can activate complement, a branch of the innate immune system, and cause adverse side-effects. Recently, we employed an in vitro hemolysis assay to measure the serum complement activity of perfluorocarbon nanoparticles that differed by size, surface charge, and surface chemistry, quantifying the nanoparticle-dependent complement activity using a metric called Residual Hemolytic Activity (RHA). In the present work, we have used a decision tree learning algorithm to derive the rules for estimating nanoparticle-dependent complement response based on the data generated from the hemolytic assay studies. Our results indicate that physicochemical properties of nanoparticles, namely, size, polydispersity index, zeta potential, and mole percentage of the active surface ligand of a nanoparticle, can serve as good descriptors for prediction of nanoparticle-dependent complement activation in the decision tree modeling framework.
RESUMO
One aspect of describing contamination in an alluvial aquifer is estimating changes in concentrations over time. A variety of statistical methods are available for assessing trends in contaminant concentrations. We present a method that extends trend analysis to include estimating the coefficients for the exponential decay equation and calculating contaminant attenuation half-lives. The conceptual model for this approach assumes that the rate of decline is proportional to the contaminant concentration in an aquifer. Consequently, the amount of time to remove a unit quantity of the contaminant inventory from an aquifer lengthens as the concentration decreases. Support for this conceptual model is demonstrated empirically with log-transformed time series of contaminant data. Equations are provided for calculating system attenuation half-lives for non-radioactive contaminants. For radioactive contaminants, the system attenuation half-life is partitioned into the intrinsic radioactive decay and the concentration reduction caused by aquifer processes. Examples are presented that provide the details of this approach. In addition to gaining an understanding of aquifer characteristics and changes in constituent concentrations, this method can be used to assess compliance with regulatory standards and to estimate the time to compliance when natural attenuation is being considered as a remediation strategy. A special application of this method is also provided that estimates the half-life of the residence time for groundwater in the aquifer by estimating the half life for a conservative contaminant that is no longer being released into the aquifer. Finally, the ratio of the half-life for groundwater residence time to the attenuation half-life for a contaminant is discussed as a system-scale retardation factor which can be used in analytical and numerical modeling.