Fingernail form as a post-extraction guide for selecting the maxillary central incisor tooth form in the Saudi Arabian population: A novel application of CAD software.

Background: Human teeth and nails have demonstrated similar factors genetically, embryologically, and morphologically. The fingernail form may be used as a post-extraction guide for selecting the tooth form. Aim: To evaluate the correlation between the maxillary left central incisor tooth form with the left index, middle, and ring fingernails in the Saudi Arabian population. Materials and Methods: A descriptive cross-sectional study with purposive sampling was done among 200 Saudi participants. The maxillary left central incisor and the left index, middle, and ring fingernail were scanned using an intraoral scanner. The 3D digital images were converted to 3D models. The tooth form (tooth length, tooth width, and tooth shape) and nail form (nail length, nail width, and nail shape) were measured using the CAD software. Pearson's correlation coefficient and T-test were used for statistical analysis (P < 0.05). Result: A significant correlation exists between tooth length and nail length, with a maximum correlation toward index nail length (P = 0.015). A significant correlation exists between tooth width and nail width, with a maximum correlation toward ring nail width (P = 0.002). A significant correlation exists between tooth length and nail length in females with a maximum correlation toward index nail length in females (P = 0.001). The mean percentage of similarity between tooth and nail shape (index, middle, and ring) in males and females shows a highly significant difference between them (P = 0.001). The results indicate that the tooth and nail shape are similar (70 to 90%) based on the criteria set by the software. Conclusion: A definite correlation exists between maxillary central incisor tooth form and fingernail form. The fingernail form may be used as a post-extraction guide for selecting the maxillary central incisor tooth form in the Saudi Arabian population.

A comparative study on the influence of third point of reference on condylar guidance settings in a semi-adjustable articulator with lateral cephalographs: An in-vivo study.

AIM: The study aimed to evaluate the effect on condylar guidance values with casts mounted by facebow transfer (Guichet's point as the third point of reference), Bonwill's, and radiographic method. MATERIALS AND METHODS: The dentulous subject's casts were mounted to a semi-adjustable articulator (Artex Type AR) after facebow transfer with Guichet's point as the third point of reference and also by Bonwill's method. Protrusive records made earlier were utilized to program the right and left side condylar guidance value. Two digital lateral cephalograms were made, one in centric relation, and one in protrusion and the condyles were traced and overlapped. The condylar guidance readings obtained by Bonwill's method, facebow transfer, and lateral cephalometric tracings were compared in this study using the SPSS (Version 17.0 Illinois, Chicago, USA) software. RESULTS: The condylar guidance values obtained from Bonwill's and the facebow transfer method (p < 0.001) was lesser than the radiographic method. The values obtained by casts mounted to the Bonwill's method differed significantly (p < 0.001) from the other two methods. CONCLUSION: The values obtained by facebow transfer were closer to the radiographic values whereas the values obtained by Bonwill's method mounting differed markedly.

Use of MTT Assay for Proliferation of U937 Cell Line and its Inter-rater Reliability - Best Taken with a Grain of Salt.

Background Biomedical research frequently employs immortal human cell lines to study various physiological and pathological topics of interest. An important problem that is often ignored is the question of reliability of observations made while using a particular assay on cell lines, especially MTT Assay. Recent studies have questioned its reliability by highlighting the diverse intracellular environmental factors that affect accuracy of MTT assay results. Objective To assess the inter-rater reliability of MTT assay while observing the differential effect of addition of fetal bovine serum to the culture medium on proliferation of U937 cell line using Intraclass correlation coefficient. Method U937 cell suspension prepared by the tutor to maintain uniformity of immortalized cell line was used. Four trainee investigators who participated in the study underwent rigorous training in pipetting and plating methods for four consecutive days prior to start of the experiment. Each trainee investigator followed the same protocol and all procedures were conducted simultaneously. At the end of the experiment, inter observer reliability was calculated. Result Interrater reliability calculated by using intra class correlation coefficient with two way mixed effect model was found to be poor (p = 0.173). For growth with 10% fetal bovine serum, inter-rater reliability was 0.258 for each item and 0.58 for the average of the triplicates, whereas for 0% fetal bovine serum it was 0.374 for each item and 0.70 for average of the values (p=0.104). Conclusion Though MTT assay is considered the gold standard test for assessment of cell proliferation and viability, the inter-rater reliability of this assay might be poor and needs further investigation.

Students' Perception of Online Platform Based Tutorial in Physiology Curriculum.

Tutorial is one of the effective ways of interactive small group teaching activity sessions. The tutorial is a short series of classes that is ideally conducted in the small group. The use of media in the form of online platforms in tutorials could be very useful. Aims of the study to evaluate the response and perception of students about the small group learning activities like Online platform tutorials in the medical undergraduate Physiology curriculum. This was the cross-sectional study done in the Physiology Department, AIIMS, Bhopal, India from 2022 to 2023 which involved first-year MBBS students (n=39). Five online platforms-based tutorials were taken whereby five topics- one for each system were discussed. In this form of tutorial, 20 to 25 minutes were focussed on discussion, followed by assessment by multiple choice questions through an online platform. Perceptions of students were assessed through a predesigned validated questionnaire, which involved open-ended and close-ended questions. Likert scale questionnaires were also used. The chi-square test and odds ratio was used to see the difference between categorical variable (yes vs. no). The mean and Median score of the Likert scale was reported. The major issue faced was the failure to promote writing skills followed by connectivity problems. Seventy-eight percent (78.0%) of participants liked this form of tutorial, and 94.0% of participants advocated using it in the future. Online platform-based tutorials motivated 84.0% of students to learn topics in a better way. However, 41.0% feared that it does not involve communication and 92.0% opined that writing skill is not promoted. Online platform-based tutorials should be used in the Physiology curriculum. However, infrastructure in the form of prior training and internet connection must be provided. Conventional forms of tutorial should also be used to promote communication and writing skills.

Effect of Music Tempo on Pattern Reversal Visual Evoked Potential in Young Adults-Multisensory Stochastic Resonance: A Pilot Study.

Stochastic resonance (SR) is a phenomenon in which an intermediate level of noise enhances the response of a weak input signal. This phenomenon is experimentally used for rehabilitation and treatment of visuomotor disorders associated with low amplitude and higher latency P100 VEPs. We presupposed that shortly it will be possible to develop and design new devices that enable multisensory SR in the audio-visual modality for human sensorimotor enhancement and rehabilitation purposes. This study was newer in which the auditory noise was used in the form of song of different tempos and their amplitude and latency of P100 PRVEP (pattern reversal visual evoked potential) was compared. Whether the different tempos song may affects the PRVEP. This study was conducted on 57 healthy volunteers in AIIMS Bhopal. The subjects were placed in front of a computer displaying a checkerboard 8x8 size. Electrodes were pasted on their scalp. The average latencies and amplitudes of the PRVEP were recorded at resting, then with the stimulus of acoustic noise in the form of low and high tempo song. The PRVEP were recorded for each event. We found that low- and high-tempo songs do not affect the latency component of PRVEP, while it significantly affects amplitude. Music therapy can help with vision rehabilitation, especially in patients with low amplitude PRVEP. A more significant number of samples for further study in this field in multisensory SR with visual evoked potentials (VEP) are necessary. We can also conclude that a sound-free environment is essential during VEP recording.

Changes in Peripheral Brachial Blood Pressure from Supine to Lateral Decubitus Position in Hypertensive and Normotensive Subjects.

Arterial blood pressure is crucial for supplying the nutritional demands of the all body tissues and is also under tight control for the same. The day to day stress impacts the blood pressure (BP), which is effectively corrected by the baroreceptors mechanism. The effect of lateral decubitus position on the blood pressure with respect to supine position needs to be evaluated as it impacts the haemodynamics. The peripheral brachial blood pressure changes are studied among the normotensives and hypertensives with its significance from change in supine to lateral decubitus position. Sixty (60) normotensives and 60 hypertensive subjects were evaluated for arterial blood pressure measurement using mercury sphygmomanometer by gold standard auscultatory method in the supine, right and left lateral decubitus position. The same observer measured the blood pressure at the similar point of time for all the participants. The Supine and lateral decubitus Systolic and diastolic blood pressure (Mean±SD) parameters were recorded and compared using the paired 't' test (Supine Normotensives SBP 112.5±16.9, DBP 70.1±17.7 mm of Hg; Hypertensives Stage I SBP 130.2±14.5, DBP 85.2±2.8 mm of Hg; Hypertensive Stage II SBP 152.6 ±17.5, DBP 98.4±9.5mm of Hg). The mean and percentage change in systolic and diastolic BP for Normotensives from supine to right lateral position- SBP 11.6 mm of Hg (10.3%), DBP 8.8 mm of Hg (12.5%) and supine to left lateral position SBP 12.5mm of Hg (11.1%), DBP 9.9 mm of Hg (14.1%) p<0.05, for Hypertensives Stage I supine to right lateral position SBP 13.8 mm of Hg (10.5%), DBP 14.8 mm of Hg (17.37%) and supine to left lateral position SBP 13.5 mm of Hg (10.3%), DBP 12.3 mm of Hg (14.13%) p<0.05 and for Hypertensive Stage II from supine to right lateral position SBP 22.6 mm of Hg (14.8%), DBP 16.6 mm of Hg (16.8%), form supine to left lateral position SBP 12.3mm of Hg (8%), DBP 14.7mm of Hg (14.9%) p<0.01) changed significantly among the all the groups with respect to change from supine to lateral decubitus position. The fall in diastolic BP was of greater magnitude than the systolic BP. The right lateral decubitus position was dominant among the hypertensives for producing the change as compared to left lateral decubitus position. However among the normotensives it was left lateral decubitus which produced greater reduction in blood pressure. The lateral decubitus position definitively reduces the blood pressure as compared to supine position and the changes produced can't be simply ascribed to the hydrostatic effect of gravity on blood column. Sleeping on lateral side could thus be advantageous in reducing the arterial blood pressure.

Innovative Approach of Learning Physiology through National Level Quiz.

Quiz has been considered an innovative practice of teaching. Self-directed learning is promoted by the quiz and this would help in better retention and help in clearing the concept of the students. The study aimed to assess the perception of the National level quiz conducted by the Physiology Department at AIIMS, Bhopal amongst participants from all over India through the questionnaire-based survey. This cross-sectional study was done based on questionnaire-based feedback from the students (29 number) participating in National Physiology Quiz. A pre-designed, structured, pre-validated questionnaire that includes close-ended questions based on the Likert scale and open-ended questions was distributed to participants and their responses were recorded. The Mean ±SD scores and median scores of 20 feedback questionnaires were evaluated using Microsoft excel software. The majority of the students (Mean more than 6) agreed that attempting/ attending the majority of the rounds was a good learning experience. The further quiz developed an interest in innovatively reading Physiology, it developed novel ideas and develop an interest in research activities and it helped us to improve communication and would help in clinical practice. Participants suggested conducting a screening round online (86.0%) and an audio-visual round (41.0%) was liked most, followed by a rapid-fire round (31.0%). National level quiz is an enjoyable activity for students and it promotes active learning in the students.