Light-emitting plants development by inoculating of Vibrio campbellii RMT1 on the rhizospheric zone of Aglaonema cochinchinense.

The concept of utilizing light-emitting plants (LEPs) as an alternative to traditional electricity-based lighting has garnered interest. However, challenges persist due to the need for genetic modification or chemical infusion in current LEPs. To address this, researchers have investigated the interaction between plants and luminous bacteria, specifically Vibrio campbellii, which can efficiently be translocated into Aglaonema cochinchinense tissues through the roots to produce LEPs. This study concentrated on examining light intensity and enhancing luminescence by growing plants and spraying them with various media substances. The results indicated that V. campbellii successfully translocated into the plant tissue via the root system and accumulated a high number of bacteria in the stems, approximately 8.46 × 104 CFU/g, resulting in a light-emitting intensity increase of 12.13-fold at 48 h, and then decreased after 30 h. Interestingly, luminescence stimulation by spraying the growth medium managed to induce the highest light emission, reaching 14.84-fold at 48 h, though it had some negative effects on the plant. Conversely, spraying plants with CaCl2 on the leaves prolonged light emission for a longer duration (42 h after spraying) and had a positive effect on plant health, it maintained ion homeostasis and reduced-MDA content. This study highlights the potential of using V. campbellii and CaCl2 spraying for the future development of practical light-emitting plants.

Using stacked pot connection of wetland microbial fuel cells to charge the battery: Potential and effecting factor.

In the practical application of wetland microbial fuel cells (WMFCs), suitable designs and stacked connection systems have consistently been employed to increase and harvest power generation. Our study compares different WMFCs designs and demonstrates that the cylinder pot design outperforms the small hanging pot design in terms of electrical energy production. Moreover, power generation from the cylinder pot can be further optimized through separator modification and stacked connections. The stacked WMFCs design exhibited no voltage reversal, with an average power output ranging from 0.03 ± 0.01 mW (single pot) to 0.11 ± 0.05 mW (stacked connection of 5 pots) over a 60-day operational period. Additionally, our study identifies distinct patterns in both anodic and cathodic physiochemical factors including electrical conductivity (EC), pH, and nitrate (NO3-), highlighting the significant influence of plant involvement on altering concentrations and levels in different electrode zones. The WMFCs bioelectricity production system, employing 15 pots stacked connections achieves an impressive maximum power density of 9.02 mW/m2. The system's practical application is evidenced by its ability to successfully power a DC-DC circuit and charge a 1.2 V AAA battery over a period of 30 h, achieving an average charging rate of 0.0.2 V per hour.

Exploring bioluminescence in Aglaonema: Investigating Vibrio campbellii translocation and plant responses under CaCl2 stimulation.

The feasibility of creating light-emitting plants by immobilizing Vibrio campbellii RMT1 on the rhizospheric zone of Aglaonema sp. 'Banlangngoen' was investigated in depth, including bacteria translocation and plant response. Results from scanning electron microscope showed that an inorganic salt-containing medium affected the root. However, transmission electron microscope results displayed bacteria translocation through the root to the leaf and colonized in the cytosol of vascular tissues. Bacteria cell counts exhibited high colonization in the root zone, approximately 3.65 × 106 CFU/mL, resulting in a light-emitting intensity increase of 23.68-fold higher than the control after the first week. Nevertheless, light microscope revealed that inorganic salts in the culture medium led to enlarged air spaces, resulting in leaf and stalk withering. Notably, spraying plants with calcium chloride (CaCl2) solution effectively mitigated salt stress, activated luminescence, and facilitated bacterial movement from roots to leaves. Additionally, CaCl2 contributed to ongoing salinity reduction in the culture medium, as evidenced by reduced malondialdehyde levels, alongside increased indole-3-acetic acid and salicylic acid concentrations, indicating plant defense responses. The interaction between plants and luminescent bacteria demonstrated the potential for producing glowing plants following CaCl2 application, addressing salinity stress, enhancing luminescence, and maintaining plant growth.

Possibility to Apply Strontium Aluminate to Produce Light-Emitting Plants: Efficiency and Safety.

Light-emitting plants (LEPs) provides light in areas without electricity. The phosphorescent compound was used as a lighting material for LEP development. However, using the phosphorescent compound for LEPs development required optimization and phytotoxicity evaluation. Strontium aluminate (SrAl2 O4 ) is a phosphorescent compound that can glow for a long time and is easily recharged by visible light. In this study, using SrAl2 O4 to develop LEPs was evaluated. Additionally, plant stress under SrAl2 O4 was investigated. Metabolomic analysis can explain the possible mechanism of plants' stress under SrAl2 O4 . After, injecting 3 mL of 5 % (w/v) SrAl2 O4 products 1, 2, and 3 into the stem of Ipomoea aquatica, the result showed that SrAl2 O4 products 2 and 3 caused oxidative stress. The metabolomic analysis also indicated that I. aquatica responded to SrAl2 O4 product 1 by increasing pipecolic acid and salicylic acid, while I. aquatica injected with SrAl2 O4 products 2 and 3 showed a decrease in salicylic acid around 0.005 and 0.061-fold, respectively, compared to control plants. and an excess accumulation of MDA around 10.00-12.00 µmol g-1 FW. A 15 % concentration of SrAl2 O4 can be used for LEPs development, enabling photoemission 18-fold for 50 min. SrAl2 O4 product 1 has the potential to be a material for LEPs.

Effects of high-strength landfill leachate effluent on stress-induced microalgae lipid production and post-treatment micropollutant degradation.

This research investigates the effects of landfill leachate effluent concentrations from moving bed biofilm reactor (MBBR) on stress-induced Chlorella vulgaris and Scenedesmus armatus lipid production and post-treatment micropollutant degradation. The effluent concentrations were varied between 25%, 50%, 75%, and 100% (v/v). The landfill leachate influent was treated using two-stage moving bed biofilm reactor under 24 h and 18 h hydraulic retention time (HRT). The results indicated that the effluent concentration was positively correlated with the stress-induced microalgae lipid production in the post-treatment of residual micropollutants. C. vulgaris and S. armatus completely remove residual micropollutants in the effluent. The superoxide dismutase and peroxidase activity were positively correlated with the cellular lipid content. The lipid content of C. vulgaris and S. armatus cultivated in the 18 h HRT effluent were 31-51% and 51-64%, while those in the 24 h HRT effluent were 15-16% and 5-19%. The optimal condition of microalgae cultivation for the post-treatment of residual micropollutants was 50-75% (v/v) effluent concentrations under 18 h HRT, achieving the highest lipid production of 113-116 mg/L for C. vulgaris and 74-75 mg/L for S. armatus. Essentially, the MBBR landfill leachate effluent holds promising potential as a substrate for microalgae lipid production.

Remediation of algal cells, PO43-, and NO3- from eutrophic wastewater using Echinodorus cordifolius in zigzag-horizontal subsurface constructed wetlands.

The pollutant removal efficiency of traditionally constructed wetlands (CWs) is often limited due to low interaction time between wastewater and the CW matrix (plants, microbes, and substrates). A zigzag-horizontal subsurface flow constructed wetland with effluent recirculation (Z-HSSF + ER) was developed to improve removal efficiency. Echinodorus cordifolius plants were used in this study. The efficiency of the systems was evaluated using eutrophic wastewater. The results showed that the developed systems exhibited the high removal efficiency of algal cells, PO43-, and NO3- (97%, 70%, and 100%, respectively), within 5 days. Algal cells were removed by the interception mechanism of gravel and zigzag baffles. PO43- and NO3- in the eutrophic wastewater was mainly removed by E. cordifolius including rhizobacteria and other microorganisms. The long flow pathway created by the installation of zigzag baffles combined with effluent recirculation provides high dissolved oxygen (DO) in the systems and increases the interaction time between wastewater and the CW matrix, thus improving the pollutant removal efficiency of CWs.

Evaluation of indoor air quality in high-rise residential buildings in Bangkok and factor analysis.

High-rise residential developments are rapidly increasing in urban areas. Smaller residential units in this high rise bring a reduction in windows, resulting in poor indoor air ventilation. In addition, materials used in interiors can emit volatile organic compounds (VOCs), which can significantly affect human health. Since people spend 90% of their time indoors, an evaluation of indoor air quality is especially important for high-rise residential buildings with an analysis of determining factors. This study aims to measure the concentrations of VOCs, formaldehyde, and particulate matter (PM2.5 and PM10) in 9 high-rise residential buildings in Bangkok by using the accidental sampling method (n = 252) and to investigate possible important determining factors. The results show that the average concentrations of VOCs, formaldehyde, PM2.5, and PM10 in 9 high-rise residential buildings were at good to moderate levels in the indoor air quality index (IAQI) and that high pollutant concentrations were rarely found except in new constructions. Moreover, it was found that the age of buildings shows strong correlations with all pollutants (p value < 0.0001). Old buildings showed significantly lower pollutant concentrations than new and under-construction buildings at a 95% confidence level. The findings from this investigation can be used as part of sustainable well-being design guidelines for future high-rise residential developments.

Effect of exogenous catechin on alleviating O3 stress: The role of catechin-quinone in lipid peroxidation, salicylic acid, chlorophyll content, and antioxidant enzymes of Zamioculcas zamiifolia.

Ozone (O3) can cause oxidative stress in plants and humans. Catechin is an antioxidant that enriches tea and can probably increase O3 tolerance in plants. To investigate the mechanism of catechin to alleviate O3 stress in plants, Zamiocalcus zamiifolia (an efficient plant for O3 phytoremediation) was sprayed with 5 mM catechin and was used to expose O3 (150-250) under long-term operation (10 cycles). We investigated whether exogenous catechin could enhance O3 removal and alleviate O3 stress through a balanced redox state in plants. Z. zamiifolia sprayed with catechin exhibited higher O3 removal (80.27±3.12%), than Z. zamiifolia without catechin (50.03±2.68%). O3 in the range of 150-250 ppb led to stress in plants, as shown by an increased malondialdehyde content (MDA) and salicylic acid (SA). Whereas under the presence of O3, exogenous catechin could maintain the MDA content and inhibit SA accumulation. Under Z. zamiifolia+catechin+O3 conditions, catechin reacted with O3, which led to the formation of catechin-quinone. The formation of catechin-quinone was confirmed by the depletion of reduced glutathione content (GSH). This catechin-quinone could induce GST and APX genes that are up-regulated approximately 35- and 5-fold, respectively. Hence, Z. zamiifolia+catechin+O3 conditions had higher performance for coping with oxidative stress than did Z. zamiifolia+O3 conditions. This evidence demonstrates that catechin could enhance O3 removal through a balanced redox state in plant cells. Finally, the application of tea extract for enhanced O3 removal is also shown in this study.

Contribution of Bacillus cereus ERBP in ozone detoxification by Zamioculcas zamiifolia plants: Effect of ascorbate peroxidase, catalase and total flavonoid contents for ozone detoxification.

Eighteen plant species were screened for ozone (O3) removal in a continuous system. Zamioculcas zamiifolia had the highest O3 removal efficiency. To enhance O3 removal by Z. zamiifolia, adding a compatible endophytic bacteria, Bacillus cereus ERBP into Z. zamiifolia was studied. After operating under an O3 continuous system (150-250 ppb) at a flow rate of 0.3 L min-1 for 80 h, inoculated plants (74%) exhibited higher O3 removal efficiency than non-inoculated ones (53%). In addition, after O3 exposure (80 h), the population of B. cereus ERBP in inoculated plants was significantly increased in both shoots approximately 35 folds and leaves 13 folds compared to inoculated plants without O3 exposure. The results also showed that B. cereus ERBP had the ability to protect Z. zamiifolia against O3 stress conditions. The increase in B. cereus ERBP populations was attributed to the significant increase in ascorbate peroxidase (APX) and catalase (CAT) activity. In addition, increasing B. cereus ERBP populations led to raise total flavonoid contents which is one of antioxidant compounds. Increasing APX, CAT activities, and total flavonoid contents can enhance O3 detoxification in plant tissues. The mechanism of B. cereus ERBP for enhancing O3 phytoremediation was proposed in this study. The results suggested that B. cereus ERBP was a potential tool for alleviating O3 stress on Z. zamiifolia and enhancing O3 phytoremediation efficiency.

Lignin and holocellulose from coir pith involved in trimethylamine (fishy odor) adsorption.

Coir pith is a highly potential adsorbent for adsorbing trimethylamine (TMA). It harbors a higher adsorption capacity for TMA compared to commercial activated carbon (CAC). It was found that lignin and holocellulose extracted from coir pith played an important role in TMA adsorption. Lignin itself had the highest TMA adsorption capacity (269.01 mg/g) followed by holocellulose (75.43 mg/g), coir pith (14.3 mg/g) and CAC (10.26 mg/g), respectively. The pseudo-first- and second orders were applied to the kinetic data. For the adsorption of TMA by coir pith, the best fit was achieved by the pseudo-second order. Thermodynamic studies showed an endothermic and physico-chemical adsorption process between TMA and the coir pith. TMA desorption study suggested that only 14%-47% of TMA was desorbed with distilled water. In addition, Fourier transform-infrared (FT-IR) spectra showed that C-H bond (methyl group), C-O bond from phenolic alcohol and C-O bond from tertiary alcohol in lignin and holocellulose were involved in TMA adsorption. Coir pith-based filter showed high TMA adsorption efficiency (98%) and kept constant for more than 48 days in a continuous system. Pilot scale experiment, coir pith beads filter could be succesfully applied as a packing material for TMA removal. Therefore, coir pith can be used as a promising packing material for TMA treatment at contaminated site.

Inoculated Clitoria ternatea with Bacillus cereus ERBP for enhancing gaseous ethylbenzene phytoremediation: Plant metabolites and expression of ethylbenzene degradation genes.

Air pollutants especially polyaromatic hydrocarbons pose countless threats to the environment. This issue demands for an effective phytoremediation technology. In this study we report the beneficial interactions of Clitoria ternatea and its plant growth promoting endophytic bacteria Bacillus cereus ERBP by inoculating it for the remediation of 5 ppm airborne ethylbenzene (EB). The percentage efficiency for ethylbenzene removal among B. cereus ERBP inoculated and non-inoculated sterile and natural C. ternatea has also been determined. The inoculation of B. cereus ERBP has significantly increased EB removal efficiency of both sterile and natural C. ternatea. The inoculated natural C. ternatea seedlings showed 100% removal efficiency within 84 h for the aforementioned pollutant compared with the sterile inoculated C. ternatea seedlings (108 h). The degradation of EB by C. ternatea seedlings with and without B. cereus ERBP was assessed by measuring the intermediates of EB including 1-phenylethanol, acetophenon, benzaldehyde and benzoic acid. In addition, cytochrome P450s monooxygenase (CYP83D1) and dehydrogenases (LOC100783159) involved in the oxidation of hydrocarbons are well reported for their bio catalytic activities under xenobiotic stress conditions. Hence, the co-effect of the native endophyte B. cereus ERBP inoculation and EB exposure on the expression level of CYP83D1 and dehydrogenase were also determined. The targeted genes CYP83D1and dehydrogenases have shown an increased expression level under the 5 ppm of EB exposure enabling C. ternatea to withstand and remediate the pollutant.

Euphorbia milii-Endophytic Bacteria Interactions Affect Hormonal Levels of the Native Host Differently Under Various Airborne Pollutants.

This study was conducted to assess the effect of plant-native endophytic bacteria interactions on indole-3-acetic acid (IAA), ethylene levels, and hormonal balance of Euphorbia milii under different airborne pollutants. IAA levels and airborne formaldehyde removal by E. milii enhanced when inoculated with endophytic isolates. However, one isolate, designated as root endophyte 4, with the highest levels of IAA production individually, declined gaseous formaldehyde removal of plant, since it disturbed hormonal balance of E. milii, leading to IAA levels higher than physiological concentrations, which stimulated ethylene biosynthesis and stomatal closure under light conditions. However, plant-root endophyte 4 interactions favored airborne benzene removal, since benzene was more phytotoxic and the plant needed more IAA to protect against benzene phytotoxicity. As trimethylamine (TMA) was not toxic, it did not affect plant-endophyte interactions. Therefore, IAA levels of root endophyte 4-inoculated E. milii was not significantly different from a noninoculated one. Under mixed-pollutant stress (formaldehyde, benzene, TMA), root endophyte 4-inoculated E. milii removed benzene at the lowest rate, since benzene was the most phytotoxic pollutant with the greatest molecular mass. However, TMA (with greater molecular mass) was removed faster than formaldehyde due to higher phytotoxicity of formaldehyde. Plant-endophyte interactions were affected differently under various airborne pollutants.

Exogenous of different elicitors: proline and ornithine on Sansevieria trifasciata under particulate matter (PM) and volatile organic compounds (VOC).

Phytoremediation has become famous for removing particulate matter (PM) and volatile organic compounds (VOC) in situ. Plants for removing PM and VOC were associated with botanical biofilters to attract pollution to the plant. On the other hand, persistent pollution exposure can lower plant health and phytoremediation effectiveness; therefore, improving plant tolerance against stress is necessary. Various elicitors can enhance plant tolerance to certain stressors. This study aims to investigate different elicitors to maintain plant health and improve the use of plants in phytoremediation for PM and VOC pollution. This experiment used Sansevieria trifasciata hort. ex Prain under PM and VOC stress. Exogenous elicitors, such as proline, ornithine, and a commercial product, were applied to the leaf parts before exposure to PM and VOC stress. The initial concentrations of PM1, PM2.5, and PM10 were 300-350, 350-450, and 400-500 µg m-3, respectively, while the VOC concentration was 2.5-3.0 mg m-3. The plant was stressed for 7 days. The result indicated that ornithine 10 mM is vital in improving plant tolerance and inducing antioxidant enzymes against PM and VOC, while proline 50 mM and a commercial product could not reduce plant stress. This study suggests that ornithine might be an important metabolite to improve plant tolerance to PM and VOC.

Sansevieria trifasciata's specific metabolite improves tolerance and efficiency for particulate matter and volatile organic compound removal.

Phytoremediation has become famous for removing particulate matter (PM) and volatile organic compounds (VOCs), but the ability is affected by plant health. Lately, the priming technique was a simple approach to studying improving plant tolerance against abiotic stress by specific metabolites that accumulated, known as "memory", but the mechanism underlying this mechanism and how long this "memory" was retained in the plant was a lack of study. Sansevieria trifasciata was primed for one week for PM and VOC stress to improve plant efficiency on PM and VOC. After that, the plant was recovered for two- or five-weeks, then re-exposed to the same stress with similar PM and VOC concentrations from cigarette smoke. Primed S. trifasciata showed improved removal of PMs entirely within 2 h and VOC within 24 h. The primed plant can maintain a malondialdehyde (MDA) level and retain the "memory" for two weeks. Metabolomics analysis showed that an ornithine-related compound was accumulated as a responsive metabolite under exposure to PM and VOC stress. Exogenous ornithine can maintain plant efficiency and prevent stress by increasing proline and antioxidant enzymes. This study is the first to demonstrate plant "memory" mechanisms under PM and VOC stress.

Application of Acinetobacter indicus to promote cigarette smoke particulate matter phytoremediation: removal efficiency and plant-microbe interactions.

Particulate matter (PM) is one of the most hazardous atmospheric pollutants. Several plant species show high potential to reduce air pollutants and are widely used as green belts to provide clean outdoor spaces for human well-being. However, high PM concentrations cause physiological changes and stress in plants. In this study, 11 species of Thai native perennial plants were exposed to PM generated from tobacco smoke. Wrightia religiosa (Teijsm. & Binn.) Benth. ex Kurz, Bauhinia purpurea DC. ex Walp. and Tectona grandis L.f. reduced PM effectively (which is in the typical range of 43.95 to 52.97%) compared to other plant species. In addition, the responses of perennial plants under PM stress at the proteomic level were also evaluated. Proteomic analysis of these three plant species showed that plants respond negatively to high PM concentrations, such as reducing several photosynthetic-related proteins and increasing plant stress response proteins. To improve PM phytoremediation efficiency and reduce plant stress from PM, perennial plant-microbe interactions were investigated. W. religiosa was inoculated with Acinetobacter indicus PS1, and high biosurfactant-producing strains clearly showed a higher PM removal efficiency than non-inoculated plants (9.48, 9.5 and 12.6% for PM1.0, PM2.5 and PM10, respectively). Inoculating W. religiosa with A. indicus PS1 maintained chlorophyll a and b concentrations. Moreover, the malondialdehyde (MDA) concentration of W. religiosa inoculated with A. indicus PS1 was lower than that of non-inoculated W. religiosa. The leaf wax content (µg/cm2) and biosurfactant (µg/cm2) of W. religiosa inoculated with A. indicus PS1 were also higher than those of non-inoculated W. religiosa. This study clearly showed that inoculating plants with A. indicus PS1 can help plants remediate PM and improve their PM stress response.

Active living wall for particulate matter and VOC remediation: potential and application.

Particulate matters (PM) and volatile organic compounds (VOCs) are the sources of toxic substances that hurt human health and can cause human carcinogens. An active living wall was applied to reduce PM and VOC contamination, while Sansevieria trifasciata cv. Hahnii, a high-performance plant for VOC removal, was selected to grow on the developing wall and used to treat PM and VOCs. The active living wall operating in a 24 m3 testing chamber showed the ability to remediate more than 90% PM within 12 h. The VOC removal can be approximately 25-80% depending on each compound. In addition, the suitable flow velocity of the living wall was also investigated. The flow rate of 1.7 m3 h-1 in front of the living wall was found as the best inlet flow velocity for the developed active living wall. The suitable condition for PM and VOC removal in the active living wall application on the real side was presented in this study. The result confirmed that the application of an active living wall for PM phytoremediation can be an alternative effective technology.

Development of light-emitting Episcia lilacina leaf by applying Vibrio campbellii RMT1 and extending the glowing by CaCl2 and yeast extract.

Glowing Episcia lilacina was generated through foliar application of the bioluminescent bacterium Vibrio campbellii RMT1. Firstly, different nutrient formulas were tested, incorporating yeast extract and various inorganic salts, such as CaCl2, MgCl2, MgSO4, KH2PO4, K2HPO4, and NaCl, in order to enhance bacterial growth and light emission. The combination of 0.15% of yeast extract and 0.3% of CaCl2 in a nutrient broth (NB) + 1% NaCl medium extended light emission to 24 h and resulted in higher light intensity compared to other combinations of yeast extract and inorganic salts. The peak intensity reached approximately 1.26 × 108 relative light units (RLU) at 7 h. The optimal presence of inorganic salt ions likely contributed to enhanced light emission, while the yeast extract acted as a nutrient source. Secondly, the effect of proline on salt-induced stress symptoms was investigated by applying 20 mM proline to the glowing plant. Additionally, a 0.5% agar nutrient was spread on the leaves prior to bacteria application to support bacterial growth and penetration. Exogenous proline application led to a significant accumulation of proline in plant cells, resulting in decreased malondialdehyde (MDA) levels. However, the proline accumulation also reduced the light intensity of the bioluminescent bacteria. This study demonstrates the potential for generating light on a living plant using bioluminescent bacteria. Further understanding of the interaction between plants and light-emitting bacteria could contribute to the development of sustainably light-emitting plants.

The potential of proline as a key metabolite to design real-time plant water deficit and low-light stress detector in ornamental plants.

Nowadays, people are interested to use plants, especially air-purifying plants, in residential and other indoor settings to purify indoor air and increase the green area in the building. In this study, we investigated the effect of water deficit and low light intensity on the physiology and biochemistry of popular ornamental plants, including Sansevieria trifasciata, Episcia cupreata and Epipremnum aureum. Plants were grown under low light intensity in the range of 10-15 µmol quantum m-2 s-1 and 3 days of water deficit. The results showed that these three ornamental plants responded to water deficit with different pathways. Metabolomic analysis indicated that water deficit affected Episcia cupreata and Epipremnum aureum by inducing a 1.5- to 3-fold increase of proline and a 1.1- to 1.6-fold increase in abscisic acid compared to well-watered conditions, which led to hydrogen peroxide accumulation. This resulted in a reduction of stomatal conductance, photosynthesis rate and transpiration. Sansevieria trifasciata responded to water deficit by significantly increasing gibberellin by around 2.8-fold compared to well-watered plants and proline contents by around 4-fold, while stomatal conductance, photosynthesis rate and transpiration were maintained. Notably, proline accumulation under water deficit stress could be attributed to both gibberellic acid and abscisic acid, depending on plant species. Therefore, the enhancement of proline accumulation in ornamental plants under water deficit could be detected early from day 3 after water deficit conditions, and this compound can be used as a key compound for real-time biosensor development in detecting plant stress under water deficit in a future study.

Effect of propionate-cultured sludge augmentation on methane production from upflow anaerobic sludge blanket systems treating fresh landfill leachate.

This research investigates the effect of propionate-cultured sludge augmentation on methane (CH4) production from upflow anaerobic sludge blanket systems (UASB) treating fresh landfill leachate. In the study, both UASB reactors (UASB 1 and UASB 2) contained acclimatized seed sludge, and UASB 2 was augmented with propionate-cultured sludge. The organic loading rate (OLR) was varied between 120.6, 84.4, 48.2, and 12.0 gCOD/L·d. The experimental results indicated that the optimal OLR of UASB 1 (non-augmentation) was 48.2 gCOD/L·d, achieving the CH4 production of 4019 mL/d. Meanwhile, the optimal OLR of UASB 2 was 12.0 gCOD/L·d, achieving the CH4 yield of 6299 mL/d. The dominant bacterial community in the propionate-cultured sludge included the genera Methanothrix, Methanosaeta, Methanoculleus, Syntrophobacter, Smithella, Pelotomamulum, which are the VFA-degrading bacteria and methanogens responsible for unblocking the CH4 pathway bottleneck. Essentially, the novelty of this research lies in the use of propionate-cultured sludge to augment the UASB reactor in order to enhance CH4 production from fresh landfill leachate.

Effect of airflow pattern and distance on removal of particulate matters and volatile organic compounds from cigarette smoke using Sansevieria trifasciata botanical biofilter.

Botanical biofilters can effectively remove indoor air pollution. However, to apply botanical biofilters in situ, the distance of botanical biofilter to the pollutants and airflow pattern can be important factors impacting efficiency. This study examined the removal efficiency of particulate matters (PMs) and volatile organic compounds (VOCs) from cigarette smoke, such as formaldehyde and acetone, at various distances (100 cm, 175 cm, 240 cm, and 315 cm) using a Sansevieria trifasciata botanical biofilter. The botanical biofilter was placed inside a testing room (24 m3) and exposed to cigarette smoke. The pollutants removal efficiency was evaluated for six cycles (24 h/cycle) and one cycle as a recovery period where botanical biofilter was placed under normal conditions for 30 days. Results showed that the botanical biofilter could remove 140-250 µg m-3, 147-257 µg m-3, 212-455 µg m-3 for PM1, PM2.5, and PM10, respectively, at 8 h. Total VOCs, formaldehyde, and acetone removal were 40%-65%, 46%-69%, and 31%-61% at 24 h. PMs and VOCs removal efficiency can be affected by both distance and pattern of airflow in the testing room. The highest PM1 and PM2.5 elimination appeared at 240 cm and 315 cm, while VOCs removal was high at 100 cm. Botanical biofilter creates airflow vortices around 100 cm, indicating low removal of PMs. This is the first study that demonstrated the effect of airflow patterns on different pollutants removal efficiency.