Global Role and Burden of Influenza in Pediatric Respiratory Hospitalizations, 1982-2012: A Systematic Analysis.

BACKGROUND: The global burden of pediatric severe respiratory illness is substantial, and influenza viruses contribute to this burden. Systematic surveillance and testing for influenza among hospitalized children has expanded globally over the past decade. However, only a fraction of the data has been used to estimate influenza burden. In this analysis, we use surveillance data to provide an estimate of influenza-associated hospitalizations among children worldwide. METHODS AND FINDINGS: We aggregated data from a systematic review (n = 108) and surveillance platforms (n = 37) to calculate a pooled estimate of the proportion of samples collected from children hospitalized with respiratory illnesses and positive for influenza by age group (<6 mo, <1 y, <2 y, <5 y, 5-17 y, and <18 y). We applied this proportion to global estimates of acute lower respiratory infection hospitalizations among children aged <1 y and <5 y, to obtain the number and per capita rate of influenza-associated hospitalizations by geographic region and socio-economic status. Influenza was associated with 10% (95% CI 8%-11%) of respiratory hospitalizations in children <18 y worldwide, ranging from 5% (95% CI 3%-7%) among children <6 mo to 16% (95% CI 14%-20%) among children 5-17 y. On average, we estimated that influenza results in approximately 374,000 (95% CI 264,000 to 539,000) hospitalizations in children <1 y-of which 228,000 (95% CI 150,000 to 344,000) occur in children <6 mo-and 870,000 (95% CI 610,000 to 1,237,000) hospitalizations in children <5 y annually. Influenza-associated hospitalization rates were more than three times higher in developing countries than in industrialized countries (150/100,000 children/year versus 48/100,000). However, differences in hospitalization practices between settings are an important limitation in interpreting these findings. CONCLUSIONS: Influenza is an important contributor to respiratory hospitalizations among young children worldwide. Increasing influenza vaccination coverage among young children and pregnant women could reduce this burden and protect infants <6 mo.

Influenza surveillance in 15 countries in Africa, 2006-2010.

BACKGROUND: In response to the potential threat of an influenza pandemic, several international institutions and governments, in partnership with African countries, invested in the development of epidemiologic and laboratory influenza surveillance capacity in Africa and the African Network of Influenza Surveillance and Epidemiology (ANISE) was formed. METHODS: We used a standardized form to collect information on influenza surveillance system characteristics, the number and percent of influenza-positive patients with influenza-like illness (ILI), or severe acute respiratory infection (SARI) and virologic data from countries participating in ANISE. RESULTS: Between 2006 and 2010, the number of ILI and SARI sites in 15 African countries increased from 21 to 127 and from 2 to 98, respectively. Children 0-4 years accounted for 48% of all ILI and SARI cases of which 22% and 10%, respectively, were positive for influenza. Influenza peaks were generally discernible in North and South Africa. Substantial cocirculation of influenza A and B occurred most years. CONCLUSIONS: Influenza is a major cause of respiratory illness in Africa, especially in children. Further strengthening influenza surveillance, along with conducting special studies on influenza burden, cost of illness, and role of other respiratory pathogens will help detect novel influenza viruses and inform and develop targeted influenza prevention policy decisions in the region.

Epidemiology and molecular characterization of hepatitis B virus in Luanda, Angola.

An estimated 360 million people are infected with hepatitis B virus (HBV) worldwide. Among these, 65 million live in Africa. Despite the high levels of hepatitis B in Africa, HBV epidemiology is still poorly documented in most African countries. In this work, the epidemiological and molecular characteristics of HBV infection were evaluated among the staff, visitors and adult patients (n = 508) of a public hospital in Luanda, Angola. The overall prevalence of hepatitis B core antibody (anti-HBc) and hepatitis B surface antigen was 79.7% and 15.1%, respectively. HBV infection was higher in males and was more prevalent in individuals younger than 50 years old. HBV-DNA was detected in 100% of HBV "e" antigen-positive serum samples and in 49% of anti-hepatitis Be antibody-positive samples. Thirty-five out of the 40 HBV genotypes belonged to genotype E. Circulation of genotypes A (4 samples) and D (1 sample) was also observed. The present study demonstrates that HBV infection is endemic in Luanda, which has a predominance of genotype E. This genotype is only sporadically found outside of Africa and is thought to have emerged in Africa at a time when the trans-Atlantic slave trade had stopped.

Analysis of complete nucleotide sequences of Angolan hepatitis B virus isolates reveals the existence of a separate lineage within genotype E.

Hepatitis B virus genotype E (HBV/E) is highly prevalent in Western Africa. In this work, 30 HBV/E isolates from HBsAg positive Angolans (staff and visitors of a private hospital in Luanda) were genetically characterized: 16 of them were completely sequenced and the pre-S/S sequences of the remaining 14 were determined. A high proportion (12/30, 40%) of subjects tested positive for both HBsAg and anti-HBs markers. Deduced amino acid sequences revealed the existence of specific substitutions and deletions in the B- and T-cell epitopes of the surface antigen (pre-S1- and pre-S2 regions) of the virus isolates derived from 8/12 individuals with concurrent HBsAg/anti-HBs. Phylogenetic analysis performed with 231 HBV/E full-length sequences, including 16 from this study, showed that all isolates from Angola, Namibia and the Democratic Republic of Congo (n = 28) clustered in a separate lineage, divergent from the HBV/E isolates from nine other African countries, namely Cameroon, Central African Republic, Côte d'Ivoire, Ghana, Guinea, Madagascar, Niger, Nigeria and Sudan, with a Bayesian posterior probability of 1. Five specific mutations, namely small S protein T57I, polymerase Q177H, G245W and M612L, and X protein V30L, were observed in 79-96% of the isolates of the separate lineage, compared to a frequency of 0-12% among the other HBV/E African isolates.

Epidemiology and molecular characterization of hepatitis B virus in Luanda, Angola

An estimated 360 million people are infected with hepatitis B virus (HBV) worldwide. Among these, 65 million live in Africa. Despite the high levels of hepatitis B in Africa, HBV epidemiology is still poorly documented in most African countries. In this work, the epidemiological and molecular characteristics of HBV infection were evaluated among the staff, visitors and adult patients (n = 508) of a public hospital in Luanda, Angola. The overall prevalence of hepatitis B core antibody (anti-HBc) and hepatitis B surface antigen was 79.7 percent and 15.1 percent, respectively. HBV infection was higher in males and was more prevalent in individuals younger than 50 years old. HBV-DNA was detected in 100 percent of HBV "e" antigen-positive serum samples and in 49 percent of anti-hepatitis Be antibody-positive samples. Thirty-five out of the 40 HBV genotypes belonged to genotype E. Circulation of genotypes A (4 samples) and D (1 sample) was also observed. The present study demonstrates that HBV infection is endemic in Luanda, which has a predominance of genotype E. This genotype is only sporadically found outside of Africa and is thought to have emerged in Africa at a time when the trans-Atlantic slave trade had stopped.

Estudos epidemiológicos e caracterização molecular do vírus da Hepatite B em usuários e profissionais de saúde do Hospital Divina Providência em Angola / Studies epidemiologists and molecular characterization of the virus of Hepatitis B in users and professionals of health of the Divine Hospital Step in Angola

Estima-se que, dos cerca de 360 milhões de pessoas com infecção crônica pelo vírus da hepatite B (HBV) no mundo, 65 milhões vivem na África. A escassez de dados sobre HBV em Angola incentivou a realização deste trabalho com objetivo de avaliar a freqüência de marcadores HBV e descrever os fatores de risco associados; foi também avaliada a associação do HBV com o vírus da imunodeficiência humana adquirida (HIV) e foram identifacados os principais genótipos do HBV, visando adoção de estratégias voltadas à aplicação de medidas preventivas. Empregou-se o estudo observacional descritivo do tipo transversal. Foram estudados 505 indivíduos maiores de 18 anos de ambos os sexos, que procuraram o atendimento ambulatorial ou se apresentaram voluntariamente, e consentiram em participar da pesquisa no Hospital Divina Providência, da Província de Luanda - Angola, no período de fevereiro a março de 2007. Todas as amostras foram testadas para o HBsAg, anti-HBc e anti-HBs mas somente as amostras positivas para o HBsAg foram testadas para o HBeAg e anti-HBe. A idade dos indivíduos variou de 18 a 76 anos, com média de idade de 36 anos. A prevalência geral do HBsAg, anti-HBc, anti-HBs, HBeAg e anti-HBe foi de 14,8 por cento, 79,0 por cento, 36,6 por cento, 25,3 por cento e 77,3 por cento respectivamente. A circuncisão foi o fator de risco associado à infecção. O fator de risco antecedente de acidente com sangue de outros foi considerado estatisticamente significativo quando analisado entre os profissionais de saúde. A técnica de poliformismo do tamanho dos fragmentos de restrição (RFLP) demostrou a existência de três genótipos do HBV (A, D e E), sendo o genótipo E o mais prevalente (59,3 por cento). A co-infecção HBV/HIV ficou estimada em 4 por cento.

Estudos epidemiológicos e caracterização molecular do vírus da Hepatite B em usuários e profissionais de saúde do Hospital Divina Providência em Angola / Studies epidemiologists and molecular characterization of the virus of Hepatitis B in users and professionals of health of the Divine Hospital Step in Angola

Estima-se que, dos cerca de 360 milhões de pessoas com infecção crônica pelo vírus da hepatite B (HBV) no mundo, 65 milhões vivem na África. A escassez de dados sobre HBV em Angola incentivou a realização deste trabalho com objetivo de avaliar a freqüência de marcadores HBV e descrever os fatores de risco associados; foi também avaliada a associação do HBV com o vírus da imunodeficiência humana adquirida (HIV) e foram identifacados os principais genótipos do HBV, visando adoção de estratégias voltadas à aplicação de medidas preventivas. Empregou-se o estudo observacional descritivo do tipo transversal. Foram estudados 505 indivíduos maiores de 18 anos de ambos os sexos, que procuraram o atendimento ambulatorial ou se apresentaram voluntariamente, e consentiram em participar da pesquisa no Hospital Divina Providência, da Província de Luanda - Angola, no período de fevereiro a março de 2007. Todas as amostras foram testadas para o HBsAg, anti-HBc e anti-HBs mas somente as amostras positivas para o HBsAg foram testadas para o HBeAg e anti-HBe. A idade dos indivíduos variou de 18 a 76 anos, com média de idade de 36 anos. A prevalência geral do HBsAg, anti-HBc, anti-HBs, HBeAg e anti-HBe foi de 14,8 porcento, 79,0 porcento, 36,6 porcento, 25,3 porcento e 77,3 porcento respectivamente. A circuncisão foi o fator de risco associado à infecção. O fator de risco antecedente de acidente com sangue de outros foi considerado estatisticamente significativo quando analisado entre os profissionais de saúde. A técnica de poliformismo do tamanho dos fragmentos de restrição (RFLP) demostrou a existência de três genótipos do HBV (A, D e E), sendo o genótipo E o mais prevalente (59,3 porcento). A co-infecção HBV/HIV ficou estimada em 4 porcento. (AU)

Estudos epidemiológicos e caracterização molecular do vírus da Hepatite B em usuários e profissionais de saúde do Hospital Divina Providência em Angola / Studies epidemiologists and molecular characterization of the virus of Hepatitis B in users and professionals of health of the Divine Hospital Step in Angola

Estima-se que, dos cerca de 360 milhões de pessoas com infecção crônica pelo vírus da hepatite B (HBV) no mundo, 65 milhões vivem na África. A escassez de dados sobre HBV em Angola incentivou a realização deste trabalho com objetivo de avaliar a freqüência de marcadores HBV e descrever os fatores de risco associados; foi também avaliada a associação do HBV com o vírus da imunodeficiência humana adquirida (HIV) e foram identifacados os principais genótipos do HBV, visando adoção de estratégias voltadas à aplicação de medidas preventivas. Empregou-se o estudo observacional descritivo do tipo transversal. Foram estudados 505 indivíduos maiores de 18 anos de ambos os sexos, que procuraram o atendimento ambulatorial ou se apresentaram voluntariamente, e consentiram em participar da pesquisa no Hospital Divina Providência, da Província de Luanda - Angola, no período de fevereiro a março de 2007. Todas as amostras foram testadas para o HBsAg, anti-HBc e anti-HBs mas somente as amostras positivas para o HBsAg foram testadas para o HBeAg e anti-HBe. A idade dos indivíduos variou de 18 a 76 anos, com média de idade de 36 anos. A prevalência geral do HBsAg, anti-HBc, anti-HBs, HBeAg e anti-HBe foi de 14,8 porcento, 79,0 porcento, 36,6 porcento, 25,3 porcento e 77,3 porcento respectivamente. A circuncisão foi o fator de risco associado à infecção. O fator de risco antecedente de acidente com sangue de outros foi considerado estatisticamente significativo quando analisado entre os profissionais de saúde. A técnica de poliformismo do tamanho dos fragmentos de restrição (RFLP) demostrou a existência de três genótipos do HBV (A, D e E), sendo o genótipo E o mais prevalente (59,3 porcento). A co-infecção HBV/HIV ficou estimada em 4 porcento. (AU)