Impacts of intake of trichothecenes (Fusarium sporotrichioides) for dairy calves: Effects on animal growth, oxidative and inflammatory response.

The objective of the present study was to evaluate the impacts of trichothecenes (Fusarium sporotrichioides) for dairy calves on animal growth, oxidative and inflammatory responses in the presence or absence of essential oils. Twelve calves weaned at 70 days of age were divided into 2 groups: T-C (control) and T-EO (essential oils - oregano, thyme, basil and rosemary) in the period of 40 days consuming ration contaminated by trichothecenes (500 ppb). The animals in the T-EO group received a mixture of EOs via feed at a dosage of 0.75 mL per/kg of feed. Blood collections were performed on days 1, 20 and 40 for hematological and biochemical analyses; the fecal score was performed every 2 days on a scale of 1-5 and clinical examinations were performed 3 times during the experiment period. The animals were weighed at the beginning and at the end of the experiment; euthanasia of two calves per group for macroscopic and microscopic evaluation of several tissues (spleen, liver, duodenum, jejunum, ilium, cecum and colon) was performed at the end of the experiment. The calves in the T-EO group had a tendency (P = 0.07) of higher body weight when compared to the T-C. Treatment effect and treatment vs day interaction was detected for leukocytes and granulocytes variables, demonstrating a higher count of these cells in the T-EO group on both days (20 and 40), and the same behavior occurred for the distribution amplitude of erythrocytes (RDW). The enzymes alanine transferase (ALT), aspartate transferase (AST) and gamma glutamyl-transferase (GGT) showed higher serum activity in the T-C group (days 20 and 40). The levels of thiobarbituric acid reactive substances (TBARS) were lower in the serum of animals in the T-EO group. For calves in the T-EO group, glutathione S-transferase activity was higher in serum. Haptoglobulin and C-reactive protein levels were lower on days 20 and 40 in T-EO animals when compared to the T-C group. In the macroscopic and microscopic evaluations, which were collected at the end of the experiment after slaughtering the animals, liver and intestine did not show changes for the animals in the T-EO group, unlike the animals in the T-C group, which had moderately firm diffuse consistency of the liver and edema in the mesentery, as well as oxidative stress in tissues (liver, duodenum, jejunum, ileum, cecum and colon). The results concluded that the consumption of a mixture of EOs (essential oils - oregano, thyme, basil and rosemary) minimized the negative effects caused by trichothecenes in dairy calves, thus being an alternative to improving the immunological and antioxidant condition, as well as a possible adsorbent alternative.

The addition of curcumin to the diet of post-weaning dairy calves: effects on ruminal fermentation, immunological, and oxidative responses.

Incorporating Curcumin into animal diets holds significant promise for enhancing both animal health and productivity, with demonstrated positive impacts on antioxidant activity, anti-microbial responses. Therefore, this study aimed to determine whether adding Curcumin to the diet of dairy calves would influence ruminal fermentation, hematologic, immunological, oxidative, and metabolism variables. Fourteen Jersey calves were divided into a control group (GCON) and a treatment group (GTRA). The animals in the GTRA received a diet containing 65.1 mg/kg of dry matter (DM) Curcumin (74% purity) for an experimental period of 90 days. Blood samples were collected on days 0, 15, 45, and 90. Serum levels of total protein and globulins were higher in the GTRA group (P < 0.05) than the GCON group. In the GTRA group, there was a reduction in pro-inflammatory cytokines (IL-1ß and IL-6) (P < 0.05) and an increase in IL-10 (which acts on anti-inflammatory responses) (P < 0.05) when compared to the GCON. There was a significantly higher (P < 0.05) concentration of immunoglobulin A (IgA) in the serum of the GTRA than the GCON. A Treatment × Day interaction was observed for haptoglobin levels, which were higher on day 90 in animals that consumed Curcumin than the GCON (P < 0.05). The catalase and superoxide dismutase activities were significantly higher (P < 0.05) in GTRA, reducing lipid peroxidation when compared to the GCONT. Hematologic variables did not differ significantly between groups. Among the metabolic variables, only urea was higher in the GTRA group when compared to the GCON. Body weight and feed efficiency did not differ between groups (meaning the percentage of apparent digestibility of dry matter, crude protein, and acid detergent fiber (ADF) and neutral detergent fiber (NDF). There was a tendency (P = 0.09) for treatment effect and a treatment x day interaction (P = 0.05) for levels of short-chain fatty acids in rumen fluid, being lower in animals that consumed curcumin. There was a treatment vs. day interaction (P < 0.05) for the concentration of acetate in the rumen fluid (i.e., on day 45, had a reduction in acetate; on day 90, values were higher in the GTRA group when compared to the GCON). We conclude that there was no evidence in the results from this preliminary trial that Curcumin in the diet of dairy calves interfered with feed digestibility. Curcumin may have potential antioxidant, anti-inflammatory, and immune effects that may be desirable for the production system of dairy calves.

Performance, body development and ingestive behavior of Nelore and crossbred heifers finished in a grazing system.

The aim of this experiment was to evaluate growth, body development and ingestive behavior of Nelore and crossbred heifers. Twenty-two contemporary heifers (eight Nelore, seven Nelore × Angus (½ Angus) and seven Nelore × Pantaneiro (½ Pantaneiro) crosses) were evaluated. The variables evaluated were weight, subcutaneous fat thickness [assessed by ultrasound in the longissimus dorsi (SFT) and biceps femoris (SFTP8)] morphometric measures and ingestive behavior (determined between 7 am and 5 pm). The daily gain differed between the breeds (P < 0.05), being greater for the ½ Pantaneiro than Nelore and equal to ½ Angus at the end of the study. The ½ Angus heifers differed (P < 0.01) from the other breeds for SFT (4.36 mm), with no difference between Nelore (2.77 mm) and ½ Pantaneiro (3.38 mm). The SFTP8 was greater (P < 0.01) (5.36 mm) in ½ Angus heifers than t others crossbreed, and greater in ½ Pantaneiro than in Nelore (4.28 vs 3.29 mm). The heart girth and rump width between the ilia were larger (P < 0.01) for the ½ Angus than ½ Pantaneiro and Nelore (169 cm; 41.6 cm, respectively). Substernal height was greatest (P < 0.01) for the Nelore (69.1 cm), reflecting the greater anterior and posterior height. The ½ Angus spent the longest time (P < 0.05) walking and less time grazing. Idleness also differed (P < 0.05) throughout the day. Crossbred heifers exhibited better performance and development than Nelore. The grazing pattern of ½ Pantaneiro heifers was identical to Nelore, but walked less, gained more weight, and exhibited better carcass finishing.

Microencapsulated herbal components in the diet of Lacaune ewes: impacts on physiology and milk production and quality.

This study aimed to determine whether the addition of a microencapsulated herbal blend (MHB) based on thymol, carvacrol, and cinnamaldehyde in dairy sheep feed would improve production efficiency, milk quality, and animal health. Thirty lactating Lacaune ewes were divided into three groups: Control (T0), 150 mg blend/kg of feed (T150), and 250 mg blend/kg of feed (T250). Milk was measured before the beginning of the experiment (d 0), at the end of the adaptation period (d 15), and during the experiment (d 20). In milk samples, was measured the composition, somatic cell count (SCC), reactive oxygen species (ROS), lipoperoxidation (LPO), and total antioxidant capacity. The MHB improved the milk production (only T150 vs. T0 sheep on d 20), productive efficiency and feed efficiency, and reduced the milk SCC (only T250 vs. T0 sheep, on d 20), ROS and tended to reduce the milk levels of LPO (only T250 vs. T0 sheep on d 20). Also, MHB reduced the blood levels of neutrophils and ROS (only T250 vs. T0 sheep on d 20) and increased total protein and globulin levels. Thus, a microencapsulated blend of thymol, carvacrol, and cinnamaldehyde improved the productive performance and milk quality of sheep.

Encapsulated pepper blend in the diet of confined Holstein bullocks: effect on ruminal volatile fatty acid profiles, growth performance, and animal health.

Dairy bulls in feedlots have been a viable alternative for dairy producers to reinforce the family's income. Aspects such as balanced diets and proper management are essential for these animals to develop and allow an economic return fully. Plant extracts are performance enhancers and ruminal and intestinal health promoters. Therefore, this study aims to evaluate whether the addition of encapsulated pepper (EP) blend (Capsicum annuum, Capsicum frutescens, and Capsicum chinense - rich in capsaicin) interferes with the volatile fatty acid profile in the rumen and enhances the growth performance of Holstein bullocks in a feedlot. For the experiment, 24 whole bullocks were used, distributed into three treatments, with eight replicates per treatment (one animal as an experimental unit, kept in an individual stall): groups T0, T200, and T400, receiving 0 mg, 200 mg, and 400 mg EP/kg of concentrate, respectively. Knowing the intake of concentrate and the average body weight during the experiment, we calculated the dose in mg/kg/day of the EP; that is, the T200 animals consumed 2.45 mg EP/kg (body weight -BW)/day; and T400 consumed 4.9 mg EP/kg BW/day. The animals from T400 presented a more significant weight gain between days 15 and 45 of confinement compared to T0 (P=0.05). This same treatment (T400) had a trend of lower weight gain between days 46 and 90 (P=0.09). Likewise, the T400 group had higher feed efficiency than T0 between days 15 and 45. Furthermore, the treatments affected the white blood cell count, with the T400 bullocks showing a higher number of neutrophils and lymphocytes. Higher levels of C-reactive protein (CRP) were measured in the serum of steers from both groups that consumed pepper (P<0.01). Interaction between treatment × day was observed for the activity of glutathione enzymes (GST and GPx) and levels of lipoperoxidation (LPO) (characterized by antioxidant stimulation) associated with the reduction in serum LPO; similar antioxidant enzymes behavior was observed in the liver. In the small intestine (jejunum), the activities of antioxidant enzymes (GST and GPx) were lower in the two groups of cattle that consumed EP, and LPO was lower. The treatments affected the concentration of acetic acid in the rumen fluid, presenting lower levels in T400 compared to T200 and similar T0 (P≤0.05). There was an interaction of day vs. treatment for propionic acid, presenting a higher concentration on day 45 at T400 than T0. These results, therefore, allow us to conclude that adding 400 mg of pepper extract can be an excellent additive for weight gain at the beginning of the experiment; however, over time, this dose of additive negatively affects weight gain. Both EP doses stimulated serum and tissue antioxidant responses, reducing lipoperoxidation. However, the 400 mg EP/kg concentrate suggests a pro-inflammatory response (leukocytosis and elevated CRP), s probably related to the high dose (i.e., between 1.7 and 2.4 g/animal/day).

Effect of physically effective neutral detergent fiber on nutrient intake and digestibility, ruminal and blood parameters, and ingestive behavior of confined beef cattle.

We aimed to evaluate the effect of levels of physically effective neutral detergent fiber from forage (NDFfor) on confined cattle's metabolism and ingestive behavior. Four crossbred steers (514.0 ± 4.54 kg BW) rumen-cannulated were used. The animals were randomly distributed in a 4×4 Latin square design, and the treatments were diets with 9.5%, 5.5%, 2.5%, and 0.0% NDF from whole plant corn silage. The trial was divided into 4 periods of 21 days. The dry matter, organic matter (OM), crude protein, neutral detergent fiber (NDF), physically effective NDF 8mm (peNDF8mm) and NDF1.18mm intakes, and the digestibility of OM and NDF showed a quadratic behavior. The rumen pH values showed a decreasing linear, and the time at pH below 5.8 increased linearly in the diets with lower NDFfor. The production of volatile fatty acids and the proportions of propionate and butyrate showed an increasing quadratic behavior. On the other hand, the proportion of acetate fitted a decreasing quadratic equation. With the lower participation of forage in the diets, the time spent on rumination activity decreased in a quadratic way, and the time spent in idleness increased in a quadratic way. The corn silage can be reduced to 135 g/kg DM in the diet to obtain a minimum of 5.5% of NDF from roughage.

Addition of açai oil during the close-up dry period of Holstein cows improves colostrum quality and immune responses of their calves.

This study evaluated of the effects of açai oil during the close-up dry period of Holstein cows on colostrum quality, as well as on the immune and antioxidant responses of their calves. Sixteen multiparous cows were assigned randomly to two treatments: 1) CONTROL (n = 8) - 4.48% of soybean oil/concentrate; 2) AÇAI (n =8) - 4.48% of açai oil/concentrate. Cows fed with açai oil had greater (P≤0.04) colostrum concentrations of immunoglobulins (Ig) G (1st and 2nd milking), IgG heavy chains, IgA (only at 1st milking), alpha-lactalbumin (1st milking), total protein, and antioxidant capacity against peroxyl radicals (only at 1st milking). Cows fed with açai oil had greater serum concentrations of globulin (only on the day of calving) and total protein (only on the day of calving) (P = 0.03). Calves born of cows fed with açai oil had greater serum concentrations of total protein (only 24 and 48 h after calving) and serum concentration of IgG heavy chain (only 24 h after calving) and globulin (only 24 and 48 h after calving) (P = 0.01). These data suggest that the addition of açai oil in the cow feed during the close-up dry period boosted immunity in their calves by altering the composition of colostrum.

Suckling behavior, performance, and Kleiber index in calves of different genetic groups under shaded and unshaded environments.

The aim of this study was to evaluate the effect of heat stress on the suckling behavior and performance of Nelore (NE), ½ Nelore × ½ Angus (NA), and ½ Nelore × ½ Pantaneiro (NP) crossbred calves, in environments shaded or unshaded, using the temperature-humidity index (THI) and Kleiber index (KI) equations. Twenty-five animals were evaluated, from 30 days of age distributed as follows: 9 NE, 8 NA, and 8 NP calves, from 7:00 A.M. to 5:00 P.M., under shaded and unshaded environments. Data were collected on number of suckling episodes per day (NS), mean suckling duration (MSD), surface body temperature (SBT), THI, weight gain, and KI. THI showed difference (P < 0.05) between environments, being lower in the shaded area. NA cattle had higher SBT (33.00 ± 2.31), differing (P < 0.05) from NE (31.71 ± 1.65) and NP (31.38 ± 1.99), and lower (P < 0.05) suckling activity, suggesting reduced thermal comfort. However, their weight gain and KI were higher, differing (P < 0.05) from the other genetic groups. NE and NP cattle did not differ (P > 0.05) in weight gain and KI. Thus, the results suggest that NA calves, despite the greater thermal discomfort, used thermolysis mechanisms to maintain higher weight gain and feed efficiency (KI) than the other groups. NP showed greater thermal adaptability, in addition to weight gain and KI similar to NE cattle.

Growth, physiology, and coccidiosis infestation of suckling beef calves grazing warm-season grasses and offered creep-feeding supplementation with or without monensin.

This study evaluated the growth, physiology, and coccidiosis infestation of suckling beef calves provided monensin and grazing limpograss (Exp. 1) or bahiagrass (Exp. 2) pastures. Treatments were randomly assigned to pastures (4 pastures/treatment; 3 cow-calf pairs/pasture in Exp. 1; 4 pastures/treatment; 10 cow-calf pairs/pair of pastures in Exp. 2) and comprised of supplementation of 0.40 kg/d of soybean meal added or not with monensin (20 mg/kg of total DM intake) for 112 and 78 days before weaning in Exp. 1 and 2, respectively. In Exp. 1, supplement DM disappearance tended (P = 0.10) to be less for calves supplemented with vs. without monensin, but treatment × day and treatment effects were not observed (P ≥ 0.18) for herbage mass (HM), herbage allowance (HA), cow body condition score (BCS), calf average daily gain (ADG), calf plasma data, and fecal coccidia egg count. In Exp. 2, forage nutritive value, HM and HA, and cow BCS did not differ (P ≥ 0.43) between treatments. Supplemental monensin did not impact (P ≥ 0.78) plasma concentrations of insulin-like growth factor 1 but increased (P ≤ 0.05) calf overall ADG and plasma concentrations on day 78 and reduced plasma concentrations of urea nitrogen (PUN) on day 78 and fecal coccidia egg count on day 78 compared to calves provided no monensin supplementation. Hence, monensin supplementation successfully improved growth performance of creep-fed suckling beef calves, when herbage mass was not a limiting factor and coccidiosis infestation occurred.

Effect of injectable trace mineral at weaning on growth and physiology of Nellore calves under feed restriction.

Calves are susceptible to a wide variety of stressors besides the seasoning low forage availability during their weaning phase. Thus, it is ideal if they receive adequate trace mineral supplementation during this period. This study evaluated the effect of injectable trace minerals (ITM) on performance, concentration of oxidative stress enzymes, and biochemical plasmatic parameters in Nellore calves during the weaning phase and under feed restriction. A total of 30 Nellore calves were used at the age of 7 ± 1 month and 176.23 ± 21.68 kg of initial body weight (BW). These animals were allocated in Urochloa decumbens pasture for 28 days and transferred to Urochloa brizantha cv Marandu pasture for another 28 days. The treatments were: Saline (saline solution; 0.9% NaCl) and ITM (Multimin®, providing: 15 mg Cu/mL, 60 mg Zn/mL, 10 mg Mn/mL, and 5 mg Se/mL). Both treatments were applied subcutaneously in the ratio of 1 mL/45 kg of BW. At the end of the experiment (d 56), animals were lighter than on d 0 in both treatments (P < 0.05). However, calves that received the ITM treatment presented lower body weight (P < 0.05) on d 56, compared to those in the control group. No significant difference (P > 0.05) was observed on the concentrations of oxidative stress enzymes. Moreover, no significant difference (P > 0.05) was observed in the plasmatic parameters; these were at normal range or slightly below the concentrations described for the species. We concluded that, in a feed restriction condition, the use of ITM in weaned calves did not improve performance, and the concentration of oxidative stress enzymes, and biochemistry variables. Therefore, we do not recommend using injectable trace minerals in the concentrations used in this study in animals under such stressful conditions.

Protective effects of silymarin in broiler feed contaminated by mycotoxins: growth performance, meat antioxidant status, and fatty acid profiles.

The aim of this study was to determine whether the inclusion of silymarin in broiler feed was able to mitigate the adverse effects of mycotoxin on growth performance, health status, liver oxidative stress, and meat fatty acid profiles. A completely randomized design with four treatments, four repetitions, and 15 chicks per repetition was used, with the following groups: (a) feed without additives (NoMyc-NoSil), (b) feed supplemented with silymarin (NoMyc-Sil), (c) feed contaminated with mycotoxin (Myc-NoSil), and (d) feed contaminated with mycotoxin and supplemented with silymarin (Myc-Sil). Growth performance, intestinal and liver health, and meat quality were assessed. The consumption of feed contaminated with mycotoxin delayed weight gain and increased the feed conversion ratio; however, the addition of silymarin prevented these adverse effects on the chicken industry. Serum ALT activity was higher in Myc-NoSil broilers than in other groups. Intake of silymarin in healthy birds increased serum globulin concentration and reduced albumin concentration and ALT and AST serum activities compared to the Myc-NoSil group. The NoMyc-Sil birds had greater villus heights and crypt depths. Luminosity and water loss by cooking were affected by mycotoxin ingestion, changes that did not occur in the meat of birds that were supplemented with silymarin. The sum of saturated and monounsaturated fatty acids in the meat did not change among treatments, unlike the sum of polyunsaturated fatty acids higher in the meat of birds that consumed silymarin. We conclude that silymarin is a potential additive in broiler feed; it reduces impairment of growth performance at the end of the productive cycle, prevents oxidative stress, improves meat quality, and increases polyunsaturated fatty acids.

Laying hens fed mycotoxin-contaminated feed produced by Fusarium fungi (T-2 toxin and fumonisin B1) and Saccharomyces cerevisiae lysate: Impacts on poultry health, productive efficiency, and egg quality.

Mycotoxins represent substantial challenges to the farming industry. These include toxins produced by Fusarium fungi, particularly trichothecenes (toxin T-2) and fumonisin (FB1). In the present study, we determined the effects of addition on Saccharomyces cerevisiae lysate (SCL) added to feed contaminated with T-2 and FB1 in terms of health, productive efficiency, and egg laying quality. We used 60 Hy-line Brown laying hens, and divided them into five groups with four repetitions per group and three birds per repetition. There was one group with no contamination with toxin (NoC). The four other groups included combinations of mycotoxin (4 ppm T-2, and 20 ppm FB1): A contamination group was used as control (the C+ group), and another two contained 500 g/ton of SCL (Detoxa Plus®) (the C + D500 group) or 1000 g/ton of SCL (the C + D1000 group). Finally, one group received feed containing 500 g/ton of Detoxa Plus® and 1000 g/ton of Uniwall® MOS 25 (the C + D500 + U1000 group). The experimental period was 84 days, divided into three productive cycles of 28 days each. The NoC hens had greater egg production than the other groups. Hens that consumed feed with SCL had greater egg production than did the C+ group. The NoC hens produced eggs with greater weights than did the C hens; however, C + D1000 and C + D500 + U1000 birds produced greater egg weights than did the C+ group. The C+ group produced lower egg masses than did the NoC and C + D500 + U1000 groups. The feed intake (FI) was lower in hens that ingested mycotoxin. The use of SCL in feed minimized the negative effects of mycotoxin on feed conversion ratio (FI/dozen). Effects of treatment were detected for feed conversion ratio (kg/kg). The hens that consumed mycotoxin had lower shell resistance and thickness compared to those in the NoC group. The red color of egg yolk was greater in the control groups. There were fluctuations in levels of liver enzymes when birds consumed mycotoxin (sometimes reduced and sometimes increased); nevertheless, the cumulative effect increased the activity of alanine aminotransferase. The serum concentration of reactive oxygen species was greater in hens that ingested mycotoxin only on d 84 compared to the NoC group. Serum glutathione S-transferase activity was greater on d 56 in C + D500 and C + D1000 hens than in the others. We conclude that, in general, the consumption of mycotoxin impaired the performance and quality of the eggs of the hens; the addition of the S. cerevisiae lysate and the addition organic acids, yeast cell wall and mineral carrier minimized some of the negative effects caused by T-2 and FB1.

Effects of the inclusion of açai oil in diet of prepartum Holstein cows on milk production, somatic cell counts and future lactation.

We measured the effects of açai oil in the diets of prepartum cows to evaluate health, milk production and quality. Sixteen Holstein cows were divided into two groups: SOY used as control, and AÇAI, test group. Occurred inclusion of 4% soybean or açai oils was provided in the concentrate starting at 20 days prepartum [d -20 to d 0 (partum-day)]. The AÇAI diet increased (P=0.01) milk production (d 10 and 20) and reduced somatic cell count (d 20). In milk, no effects were detected (P≥0.10) for concentration of fat, lactose or protein as well as in terms of serum concentration of calcium, albumin or triglycerides. AÇAI diet tended to increase (P=0.09) serum concentrations of total protein, glutathione transferase (d 4), and total antioxidant capacity (d 4 and 10) and increased (P≤0.05) globulin, gamma-glutamyl transferase, superoxide dismutase and glutathione peroxidase (d 4). Further, AÇAI diet reduced the serum concentration of creatine kinase (P≤0.05) (d 0, 4 and 10), reactive oxygen species (d 0 and 4) and lipoperoxidation (d 0) and tended to reduce aspartate transaminase activity (P=0.07; d 0 and 4). Açai oil in the diets in prepartum cows improved their health as well as milk production and quality.

Effect of a trace mineral injection at weaning on growth, antioxidant enzymes activity, and immune system in Nellore calves.

The objective of this study was to evaluate the effects of injectable trace minerals (ITM) at the time of weaning on growth, antioxidant, and immune response of Nellore (Bos indicus) calves. Weaned calves (n = 159; 213 ± 32 kg) were stratified by body weight (BW) and randomly assigned to 1 of 2 treatments: injection (1 mL/45 kg of BW) of saline or ITM. Saline solution consisted of 0.9% NaCl, whereas ITM had 60, 10, 5, and 15 mg/mL of Zn, Mn, Se, and Cu, respectively. The application of ITM increased (P ≤ 0.04) the plasma concentrations of superoxide dismutase on day 7 and plasma glutathione peroxidase on day 7 and day 21. The ITM calves had greater leukocyte concentration on day 64 (P = 0.04), whereas neutrophil and mast cell concentrations did not differ (P ≥ 0.67) between treatments. The ITM calves tended (P ≤ 0.08) to have greater concentrations of lymphocytes on day 64 and eosinophils on day 21, but not (P ≥ 0.15) monocytes, erythrocytes, hemoglobin, hematocrit, mean corpuscular volume, and mean corpuscular hemoglobin compared to saline calves. The ITM calves had less mean corpuscular hemoglobin concentration (MCHC; P = 0.02) and tended to have increased platelet concentrations on day 21 (P = 0.08). Growth performance did not differ between treatments (P ≥ 0.78). Thus, ITM at weaning did not impact growth performance, but increased plasma concentrations of antioxidant enzymes and blood platelets, improved components of the immune system, and reduced MCHC of Nellore calves.

Effects of oral administration of copper capsules on helminth control in lactating dairy sheep: An effective alternative to replace conventional antiparasitics during lactation.

Two experiments were performed to determine whether oral administration of copper oxide capsules controlled helminthic infections in Lacaune sheep without acute collateral effects on animal health. In experiment 1, 48 multiparous lactating sheep (60.1 ±â€¯8.5 kg) were stratified according to initial number of eggs (Haemonchus contortus) per gram of feces (EPG) and were assigned randomly to 1 of two treatments (24 sheep/treatment): no oral administration (control) or oral administration of two copper capsules (treated; approximately 58 mg copper/kg body weight). Blood and fecal samples were collected on days 0, 15 and 45. Animals treated with copper capsules showed lower of EPG, eosinophils, acetylcholinesterase (AChE) in whole blood, and lower butyrylcholinesterase (BChE) activity in serum. Treated sheep had higher erythrocyte numbers, hemoglobin concentrations, hematocrit, and lymphocyte numbers. In experiment 2, 12 male lambs negative for helminths and coccidia were assigned randomly to one of two treatments (six lambs/treatment): control or treated (one copper capsule; approximately 58 mg copper/kg body weight); the experiment was designed to determine whether the results of experiment 1 were due to treatment or parasitism. Blood samples were collected on days 0, 5, 10 and 15 and fecal samples were collected on days 0, 7 and 15. Treated animals showed greater concentrations of lymphocytes; however, treatment had no effect on other hemogram variables, AChE and BChE activities, or levels of alanine aminotransferase, aspartate aminotransferase, gamma-glutamyl transferase, creatinine, urea, albumin, total protein, and reactive oxygen species. These data suggest that copper capsules in dairy sheep efficiently controlled H. contortus infections. Treatment was not harmful to lambs during the first 15 days, i.e. there were no signs of acute toxicity.

Nutraceutical effect of vitamins and minerals on performance and immune and antioxidant systems in dairy calves during the nutritional transition period in summer.

We aimed to determine whether the use of injectable vitamins and minerals improves growth performance and immune and antioxidant responses in dairy calves during pre- and post-weaning period in summer. Twenty dairy calves (45 days of age) were randomized to two groups (10 each): control group (CON) and treated group [TREAT; injection providing 0.20, 0.80, 0.20, 0.10, 35 and 1 mg/kg of copper, zinc, manganese selenium, and vitamins A and E, during two periods (15 days pre- and 15 days post-weaning)]. The animals were weighed and blood samples were collected on days 1, 15, 30 and 45 of the study. Levels of serum copper, selenium, zinc, and manganese were measured on day 1; and the results showed that calves were not deficient in these minerals. The TREAT group had greater BW gain during the final third of the experiment. There was an increase in total leukocyte numbers as a result of elevation in neutrophil counts (day 45) and monocytes (days 30 and 45) in the TREAT group. This group also had lower reactive oxygen species (ROS) content (days 15, 30 and 45) and lipid peroxidation (LPO; days 15 and 45). Furthermore, the TREAT group had greater antioxidant capacity against peroxyl radicals (ACAP; days 15 and 30), activities of the enzymes glutathione peroxidase (GPx; days 15, 30 and 45) and superoxide dismutase (SOD; day 15), concentrations of total serum proteins (day 30), serum globulin (days 15 and 30), ceruloplasmin (day 15), tumor necrosis factor-alpha (TNF-α), interleukin-1, (IL-1; days 30 and 45) and interferon gamma (IFNγ; day 45), compared to CON group. High respiratory rates during hot times of the day in all study calves was suggestive of heat stress. Taken together, the data suggest that mineral and vitamins injections increased the growth performance and boosted the antioxidant and immunological systems of dairy calves during the diet transition period in summer.

Benefits of the inclusion of açai oil in the diet of dairy sheep in heat stress on health and milk production and quality.

The objective of this study was to determine whether the addition of açai (Euterpe oleracea) oil in the diets of lactating sheep under heat stress exerted beneficial effects on health as well as milk production and quality. Eighteen multiparous Lacaune sheep (2 or 3 parities; 28-30 days of lactation; average milk production of 1.7 L/sheep/day) were stratified by parity and milk production and were assigned randomly to 1 of 2 treatments (9 sheep/treatment): diet supplemented with 2% of soybean oil (SOY) or 2% of açai oil (AÇAI) in the concentrate for 14 days. The amount of oil added in the diet was equivalent to 0.65% of the total diet (dry matter basis). Blood and milk samples were collected on days 1, 10 and 14. On day 14, the AÇAI group sheep had lower serum concentrations of leukocytes, neutrophils, and lymphocytes than did the SOY group sheep. On day 14, AÇAI group sheep had lower serum concentration of triglycerides and urea, milk concentration of fat and total solid and milk lipid peroxidation than did SOY group sheep. However, on day 14, AÇAI group sheep had higher serum concentrations of glucose and globulin, serum and milk antioxidant capacity against peroxyl radicals, milk production and productive efficiency than did SOY group sheep. The fatty acids profile in milk did not differ between groups. These data suggest that açai oil improved the antioxidant activity in serum and milk and improved milk production and quality in dairy sheep under heat stress.

Use of grape residue flour in lactating dairy sheep in heat stress: Effects on health, milk production and quality.

The objective of this study was to evaluate the effects of grape residue flour (GRF) on antioxidant activities, biochemistry variables, components of the immune system and milk production and quality of Lacaune sheep in heat stress. Twenty-seven multiparous lactating sheep [50 ±â€¯1.8 days (d) milking] were stratified by initial body weight, age, date of lambing and milk production and assigned randomly to 1 of 3 treatments (9 sheep/treatment): no GRF supplementation (control group) or supplementation at 1% (10 g/kg GRF) or 2% (20 g/kg GRF) of GRF (bark and seed) in the concentrate (grains and minerals mixture). Each ewe received 0.8 kg/d of concentrate, 3.6 kg/d of corn silage, and 0.25 kg/d of Cynodon spp hay. Milk production along with blood and milk samples were collected on d 1, 10 and 15. The 2% GRF sheep had increased serum concentrations of superoxide dismutase and glutathione peroxidase activity on d 15 compared to control sheep. Over time (d 10 to 15), lipid peroxidation was reduced in 2% GRF sheep. Total serum antioxidant capacity was greater in 2% GRF sheep compared to control sheep on d 10 and 15. Superoxide dismutase and glutathione peroxidase activity in milk samples were greater in 2% GRF sheep compared to control sheep. Supplementation with GRF did not affect milk production but GRF sheep were more efficient compared to control sheep. Protein and lactose concentrations were similar between treatments, but total solids and fat concentrations were greater in 2% GRF sheep compared to control sheep on d 15. Somatic cell count was reduced in GRF sheep compared to control sheep. In summary, supplementation with 2% GRF in dairy sheep in heat stress resulted in antioxidant and anti-inflammatory responses, which improved milk quality and reduced somatic cell count and lipid peroxidation.

Chromium in the Diet of Dairy Calves: Benefits for Growth Performance, Feed Efficiency, Digestibility, and Health.

Chromium (Cr) is a mineral that helps animals subjected to stressful conditions. The suckling period is characterized by several stressful episodes, particularly during the first hours after birth and at weaning. There is little consumption of concentrate by calves in the first weeks of life; consequently, consuming any supplement added to feed would be negligible. Thus, the hypothesis was that the calves would take it in earlier if Cr were consumed in a milk replacer instead. Therefore, our study aimed to determine whether including organic Cr in calf feed (via milk replacer or concentrate) during the suckling phase would improve calf health and growth performance. Twenty-four male Holstein calves with an average age of 8 ± 4 days and 39.8 ± 6.9 kg average body weight were used. Calves were randomly divided into three groups: (a) Chromium-Milk (CR-M), receiving 4 mg Cr/animal/day via milk replacer during the 60 experimental days of suckling (n = 8); (b) Chromium-Concentrate (CR-C), receiving 4 mg Cr/animal/day via concentrate (n = 8); (c) Control (C), animals that did not receive Chromium (n = 8). The experiment lasted 75 days, divided into two well-defined stages: suckling (1-60 days) and weaning (61-75 days). Body weight weekly, daily feed intake, and blood samples taken every two weeks during the experiment were evaluated. At the end of the experiment, the apparent digestibility evaluation was carried out, with the results of weight and consumption carried out, and a feed efficiency analysis was carried out. It was observed that the inclusion of organic Cr (regardless of whether it was milk or concentrate) increased body weight gain (kg) for the CR-C group: 41.8 kg, CR-M: 40.4 kg compared to the C: 34.2 kg (p = 0.01). The protein digestibility was higher in the CR-M group (52%, p = 0.05). Cr consumption increased Cr concentrations in the serum of the calves and was higher in the first week in the animals in the CR-M group. This did not happen for the C group; however, with higher concentrate consumption, Cr concentrations increased and remained high until the end of the experiment. Glucose concentrations were higher in the groups that consumed Cr. Total protein concentrations were higher in the CR-M and CR-C groups than in group C. Immunoglobulin A concentrations were higher in groups CR-C and CR-M than in group C (days 40 and 60). In conclusion, the adding Cr to calf feed improves their health, indirectly favors growth performance, and increases protein digestibility.

Butyric acid glycerides as substitutes for antibiotics as growth enhancers in the diet of nursery piglets.

This study aimed to determine whether the addition of butyric acid glycerides as substitutes to conventional growth promoters can provide adequate zootechnical performance and intestinal health in healthy piglets in the nursery phase. We used 90 male piglets (average weight of 6.5 kg) subdivided into five treatments with six replicates per treatment. The treatments had the same basal diet: NC-negative control (without growth promoter), PC-positive control (with gentamicin, oral), PSB-protected sodium butyrate, FSB-free sodium butyrate, and TRI-tributyrin. In these animals, zootechnical performance was evaluated on days 1, 10, 20 and 39, microbiological analysis on days 14 and 39, hematocrit, blood biochemistry and intestinal histology, intestinal oxidation and antioxidation on day 39. The average daily weight gain was higher in the TRI group on days 21 to 39 in the nursery (P = 0.03), with more significant weight gain from 1 to 39 days (P = 0.05). There were higher leukocyte counts in the PC group than in the TRI group and higher lymphocyte counts in the PC treatment than in the NC or TRI groups. Escherichia coli counts were lower in the PC, followed by the PSB and TRI groups on day 39 (P = 0.01). Lower crypt depths were found in the TRI and FSB groups, followed by PC, than in the NC group (P = 0.01). Higher values for crypt villosity ratio were found in the FSB and TRI groups than in the NC group (P = 0.05). Lower lipid peroxidation was found in analyzes of serum oxidative status (LPO: P = 0.01), associated with greater activities of superoxide dismutase - SOD (P = 0.08), glutathione S-transferase - GST (P = 0.09) in PSB and TRI groups than in the NC group. In conclusion, the use of butyric acid in the form of tributyrin can be used as growth enhancers in piglets in the nursery phase.