The Empathic Process and Misconceptions that Lead to Burnout in Healthcare Professionals.

Empathy has been identified as a relevant variable in order to predict burnout in healthcare professionals. In addition, assertiveness and self-esteem have been considered relevant variables to develop empathic capacity. In the other hand, misconceptions surrounding empathy constitute a risk factor for burnout. Two adult samples (N = 252 and N = 275) were used to explore and confirm the underlying structure of two questionnaires. The Exercise of Process of Empathy (EPE) scale (18 items) confirmatory factor analysis including 5 dimensions (cognitive and emotional comprehension, attention, clarity and assertiveness), showed reasonable goodness- of-fit indices χ2(130) = 269.63, p < .001; RMSEA = .069 90% CI [0.058 - 0.079]; CFI = .965; TLI = .959. Alpha coefficient resulted .848. Common Misconceptions (EH) of empathy scale (16 items) confirmatory factor analysis, including 3 dimensions (feeling, confluence and character misconceptions) also obtained reasonable goodness-of-fit indices χ 2 (101) = 250.59, p < .001; RMSEA = .075 90% CI [0.063 - 0.087]; CFI = .952; TLI = .943. Alpha coefficient for Character resulted .727 (5 items), for Confluence .764 (5 items) and for Feeling .822 (6 items). The SEM model's R 2 resulted .303, being EH (misconceptions) a risk factor (b* = .171), EPE (empathy process) a protection factor (b* = -.183). The model partially explains how misconceptions empathy process and self-esteem (b* = -.334) relate to burnout syndrome in healthcare professionals; what is more, it heralds a potential means to prevent it.

Psychometric Properties of the Attitudinal Beliefs Questionnaire about Suicidal Behavior (CCCS-18).

The purpose of this study was to analyze psychometric properties of the Spanish version of Attitudinal Beliefs Questionnaire about Suicidal Behavior CCCS-18 (Ruiz, Navarro-Ruiz, Torrente, & Rodríguez, 2005). The participants were 277 subjects, 81.2% (225) women and 18.8% (52) men. The average age was 39.95 years old (SD = 15.9). A confirmatory factor analysis was performed to test the adequacy of the four-factor model proposed by the authors. As a result it was obtained a three-factor model (χ2/df = 1.96; CFI = .98; sRMR = .060; RMSEA = .059), with indices reflecting adequate goodness of fit. The reliability of the test using the omega coefficient showed satisfactory values (ω h = .95, CI 95% = [.94, .96]). The results indicate a close relation between the CCCS-18 dimensions and the suicidal tendency, showing discriminant validity properties. Predictive validity was also found in the significant correlations between the measures obtained in the questionnaire and a risk index resulting from the suicidal ideation predictor variables that were part of a logistic regression equation; CCCS-18 (r = .26, p < .001), F1, Legimization and terminal disease (r = .163, p < .01), F2, Moral dimension (r = .22, p < .001) and F3, Suicide itself (r = .252, p < .001). CONCLUSIONS: The CCCS-18 shows reliability and validity, as well as being a test of easy and brief application.

Polychlorinated biphenyl rhizoremediation by Pseudomonas fluorescens F113 derivatives, using a Sinorhizobium meliloti nod system to drive bph gene expression.

Rhizoremediation of organic chemicals requires high-level expression of biodegradation genes in bacterial strains that are excellent rhizosphere colonizers. Pseudomonas fluorescens F113 is a biocontrol strain that was shown to be an excellent colonizer of numerous plant rhizospheres, including alfalfa. Although a derivative of F113 expressing polychlorinated biphenyl (PCB) biodegradation genes (F113pcb) has been reported previously, this strain shows a low level of bph gene expression, limiting its rhizoremediation potential. Here, a high-level expression system was designed from rhizobial nod gene regulatory relays. Nod promoters were tested in strain F113 by using beta-galactosidase transcriptional fusions. This analysis showed that nodbox 4 from Sinorhizobium meliloti has a high level of expression in F113 that is dependent on an intact nodD1 gene. A transcriptional fusion of a nodbox cassette containing the nodD1 gene and nodbox 4 fused to a gfp gene was expressed in the alfalfa rhizosphere. The bph operon from Burkholderia sp. strain LB400 was cloned under the control of the nodbox cassette and was inserted as a single copy into the genome of F113, generating strain F113L::1180. This new genetically modified strain has a high level of BphC activity and grows on biphenyl as a sole carbon and energy source at a growth rate that is more than three times higher than that of F113pcb. Degradation of PCBs 3, 4, 5, 17, and 25 was also much faster in F113L::1180 than in F113pcb. Finally, the modified strain cometabolized PCB congeners present in Delor103 better than strain LB400, the donor of the bph genes used.

Phenotypic selection and phase variation occur during alfalfa root colonization by Pseudomonas fluorescens F113.

During colonization of the alfalfa rhizosphere, Pseudomonas fluorescens F113 undergoes phenotypic variation, resulting in the appearance of colonies with different morphology. Among phenotypic variants, three isolates, C, F, and S were selected, with the C variant showing colony morphology identical to that of the inoculated wild-type strain and F and S having a translucent and diffuse morphology. Phenotypic variants F and S were shown to preferentially colonize distal parts of the roots and showed alterations in motility, swimming faster than the C variant and swarming under conditions that did not allow swarming of the C variant. The motility behavior correlated with overproduction of the fliC-encoded protein flagellin but not with hyperflagellation. Flagella of the F and S variants were several times longer than those of the C variant, and overproduction of flagellin was regulated at the transcriptional level. Variant F showed alterations in traits that have been shown to be important for rhizosphere colonization, such as siderophore, cyanide, and exoprotease production, and these phenotypes were complemented by a cloned gacA. Sequence analysis of the gacA alelle in variant F suggested selection of the phenotype in the rhizosphere. Variant F was also affected in other phenotypes, such as lipopolysaccharide structure and flocculation in unshaken liquid medium, which were not complemented by the gacA or gacS gene. Mutation of the F113 sss gene, encoding a site-specific recombinase, showed that most of the phenotypic variation was due to the activity of this recombinase, indicating that phase variation occurs during rhizosphere colonization.