Migraine screen questionnaire: further psychometric evidence from categorical data methods.

BACKGROUND: Psychometric investigations of tools used in the screening of migraine including the migraine screen questionnaire (MS-Q), using an adequate statistical approach is needed. We assessed the psychometric properties of the migraine screen questionnaire (MS-Q) using categorical data methods. MATERIAL AND METHODS: A total of 343 students at Mizan-Tepi University, Ethiopia, age range = 18-35 years were selected by a simple random sampling method to participate in a cross-sectional study. The respondents completed the MS-Q, a semi-structured socio-demographic questionnaire, and a visual analog scale for attention (VAS-A). RESULTS: The cumulative variance rule (> 40%), the Kaiser's criteria (Eigenvalue> 1), the Scree test and, the parallel analysis (minimum rank) identified a 1-factor model for the MS-Q with the factor loadings in the range of 0.78 to 0.84. Fit indices favored a 1-factor model of the MS-Q as indicated by comparative fit index (0.993), weighted root mean square residual (0.048), root mean square error of approximation (0.067), the goodness of fit index (1.00), and non-normed fit index (0.987). The values of the Factor Determinacy Index (0.953), marginal reliability (0.909), H-latent (0.909), H-observed (0.727), explained common variance (0.906) and the mean item residual absolute loadings (0.225) further complimented finding of the 1-Factor model. McDonald's Omega (0.903) suggested adequate internal consistency. Discriminative validity was supported by significantly higher scores for the total and all the MS-Q items except one among those with complaints of attention. CONCLUSION: The categorical methods support the psychometric validity of the MS-Q in the study population.

Evaluation of Antidiabetic and Antioxidant Activity of Leaf Extract and Solvent Fractions of Hypoestes forskaolii (Val) (Acanthaceae) in Mice.

BACKGROUND: Currently, there is a demand for new antidiabetic drugs from natural therapeutic agents, and diabetes mellitus disease is global epidemic. The leaf of Hypoestes forskaolii has been used in the traditional health system for the management of diabetes mellitus in Ethiopia and Eritrea. The aim of this study was to evaluate in vivo antidiabetic, antihyperlipidemic and in vitro antioxidant activity of leaf extract and solvent fractions of the leaf of H. forskaolii (Vahl) in mice. METHODS: The blood glucose-lowering activities of leaf extract and solvent fractions of the leaf of H. forskaolii were screened in the normoglycemic, glucose loaded, and streptozotocin-induced diabetic mice models. In the treatment of streptozotocin-induced diabetic mice with leaf extract and solvent fractions, weight and lipid profile was measured. Antioxidant activity of the plant leaf extract was determined using DPPH assay. RESULTS: The leaf extract of H. forskaolii showed significant blood glucose reduction in the normoglycemic model and glucose loaded test at doses of 200 mg/kg (34.1%, p < 0.001 and 55.5%, p < 0.001), respectively, as compared to the normal control. In the streptozotocin-induced diabetic model, extract and solvent fractions significantly (p < 0.001) reduced blood glucose level at all tested doses (100mg/kg, 200mg/kg and 400mg/kg) on the 14th day as compared to diabetic control. In addition, a significant reduction (p < 0.001) of serum TC, VLDL, LDL, TG was observed. In the antioxidant activity test, the IC50 values of extract and a standard drug (ascorbic acid) were 4.87µg/mL and 15.7µg/mL, respectively. CONCLUSION: The present study showed that the methanolic leaf extract and solvent fractions of H. forskaolii have antidiabetic, and antioxidant activity that provides a scientific support for the local use of the plant leaves in the treatment of diabetes.