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1.
Zhonghua Yan Ke Za Zhi ; 60(7): 611-617, 2024 Jul 11.
Artigo em Zh | MEDLINE | ID: mdl-38955763

RESUMO

Objective: To investigate the clinical features of acute macular neuroretinopathy (AMN) following coronavirus disease 2019 (COVID-19). Methods: This retrospective case series study included 15 patients (28 eyes) diagnosed with AMN at the Department of Ophthalmology, Peking University Third Hospital, from November 2022 to January 2023. The AMN group comprised 4 males and 11 females, with a mean age of (31.36±8.08) years. A control group of 15 individuals [5 males, 10 females; mean age (33.20±5.10) years] who had COVID-19 but did not develop AMN was also included. Data collected for all patients included best-corrected visual acuity (BCVA), slit-lamp examination, dilated fundus examination, color fundus photography, fluorescein fundus angiography (FFA), and optical coherence tomography (OCT) results. Serum cytokine levels, including interleukins (ILs), interferons (IFNs), and tumor necrosis factor-alpha (TNF-α), were measured for both groups. Results: Among the 28 eyes, severe vision loss (BCVA≤0.3) was observed in 3 eyes (10.7%), moderate vision loss (BCVA>0.3 and≤0.5) in 2 eyes (13.3%), and mild vision loss (BCVA>0.5 and≤1.0) in 23 eyes (82.1%). OCT findings in all 28 eyes revealed hyperreflectivity of the outer nuclear layer and disruption of outer retinal structure. Additionally, 3 eyes (10.7%) exhibited cotton wool spots in the posterior pole, 2 eyes (7.1%) showed mild cystoid macular edema with intraretinal hyperreflective dots, and 1 eye (3.6%) presented with paracentral acute middle maculopathy. FFA indicated retinal vasculitis in 2 cases (4 eyes, 14.3%). Serum levels of IL-4, IL-5, IFN-α, and IFN-γ were significantly higher in the AMN group compared to the control group: IL-4 [4.49 (3.66, 6.08) vs. 1.40 (0.62, 1.68) pg/ml], IL-5 [7.34 (5.04, 14.06) vs. 0.17 (0.11, 1.86) pg/ml], IFN-α [8.42 (6.31, 14.89) vs. 0.50 (0.30, 0.83) pg/ml], and IFN-γ [17.93 (12.75, 32.44) vs. 7.43 (0.00, 14.74) pg/ml], with all differences being statistically significant (all P<0.05). Conclusion: AMN following COVID-19 can present with wedge-shaped dark red lesions in the macular area, often accompanied by cotton wool spots and retinal vasculitis. Additionally, there is a significant elevation in various inflammatory cytokines in the serum.


Assuntos
COVID-19 , Doenças Retinianas , SARS-CoV-2 , Tomografia de Coerência Óptica , Humanos , COVID-19/epidemiologia , Masculino , Feminino , Adulto , Estudos Retrospectivos , China/epidemiologia , Tomografia de Coerência Óptica/métodos , Acuidade Visual , Angiofluoresceinografia/métodos , Macula Lutea/patologia , Macula Lutea/diagnóstico por imagem , Doença Aguda , Citocinas/sangue
2.
Zhonghua Xin Xue Guan Bing Za Zhi ; 51(5): 490-496, 2023 May 24.
Artigo em Zh | MEDLINE | ID: mdl-37198120

RESUMO

Objective: To explore the safety and efficacy of excimer laser coronary angioplasty (ELCA) for the treatment of degenerated great saphenous vein graft (SVG). Methods: This is a single-center, prospective, single-arm study. Patients, who were admitted to the Geriatric Cardiovascular Center of Beijing Anzhen Hospital from January 2022 to June 2022, were consecutively enrolled. Inclusion criteria were recurrent chest pain after coronary artery bypass surgery (CABG), and coronary angiography confirmed that the SVG stenosis was more than 70% but not completely occluded, and interventional treatment for SVG lesions was planned. Before balloon dilation and stent placement, ELCA was used to pretreat the lesions. Optical coherence tomography (OCT) examination was performed and postoperative index of microcirculation resistance (IMR) were assessed after stent implantation. The technique success rate and operation success rate were calculated. The technique success was defined as the successful passage of the ELCA system through the lesion. Operation success was defined as the successful placement of a stent at the lesion. The primary evaluation index of the study was IMR immediately after PCI. Secondary evaluation indexes included thrombolysis in myocardial infarction (TIMI) flow grade, corrected TIMI frame count (cTFC), minimal stent area and stent expansion measured by OCT after PCI, and procedural complications (Ⅳa myocardial infarction, no reflow, perforation). Results: A total of 19 patients aged (66.0±5.6) years were enrolled, including 18 males (94.7%). The age of SVG was 8 (6, 11) years. The length of the lesions was greater than 20 mm, and they were all SVG body lesions. The median stenosis degree was 95% (80%, 99%), and the length of the implanted stent was (41.7±16.3)mm. The operation time was 119 (101, 166) minutes, and the cumulative dose was 2 089 (1 378, 3 011)mGy. The diameter of the laser catheter was 1.4 mm, the maximum energy was 60 mJ, and the maximum frequency was 40 Hz. The technique success and the operation success rate were both 100% (19/19). The IMR after stent implantation was 29.22±5.95. The TIMI flow grade of patients after ELCA and stent implantation was significantly improved (all P>0.05), and the TIMI flow grade of all patients after stent implantation was Grade Ⅲ. The cTFC decreased significantly after ELCA (33.2±7.8) and after stent placement (22.8±7.1) than preoperative level (49.7±13.0) (both P<0.001). The minimum stent area was (5.53±1.36)mm2, and the stent expansion rate was (90.0±4.3)%. Perforation, no reflow, type Ⅳa myocardial infarction and other complications were not observed. However, postoperative high-sensitivity troponin level was significantly increased ((67.937±33.839)ng/L vs. (5.316±3.105)ng/L, P<0.001). Conclusion: ELCA is safe and effective in the treatment of SVG lesions and could improve microcirculation and ensure full expansion of stent.


Assuntos
Aterectomia Coronária , Infarto do Miocárdio , Intervenção Coronária Percutânea , Masculino , Humanos , Idoso , Estudos Prospectivos , Lasers de Excimer/uso terapêutico , Veia Safena/transplante , Constrição Patológica , Aterectomia Coronária/métodos , Angiografia Coronária , Stents , Resultado do Tratamento
3.
Soft Matter ; 18(33): 6148-6156, 2022 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-35968815

RESUMO

Helical micro-swimmers have markedly extended the reach of human beings in numerous fields, ranging from in vitro tasks in lab-on-a-chip to in vivo applications for minimally invasive medicine. The previous studies on the propulsive motility optimization of the micro-swimmers mainly focused on the distinct actuation principles (e.g., chemically powered, magnetic- or ultrasound energy-driven) and paid little attention to the structural design of these swimming machines themselves. The improvements of the structures can assist the externally powered motors in providing propulsion in a tiny scale and satisfy the agile locomotion demands. This paper presents the design, mechanics modeling and available experiments of a novel type of hierarchical helical swimming robot that significantly enhances the motility of the helix-based swimmers. Validated by the resistive force theory, our numerical model can well analyze the mechanical properties with a variety of geometric parameters. The motion performance of the hierarchical and conventional helical structures in low Reynolds regimes is presented, highlighting the advantages of hierarchical swimmers over the existing typical swimmers. In addition, the stability and resilience of the hierarchical swimmers can be maintained at a decent level. Moreover, the variable forward velocity resulting from the combined hierarchical structures is investigated here, which can thereby serve as a reliable design strategy. The proposed hierarchical helical design enables enticing opportunities for various device systems of medical robots and bio-integrated electronics.


Assuntos
Magnetismo , Natação , Humanos , Locomoção
4.
Zhonghua Yan Ke Za Zhi ; 58(12): 1033-1038, 2022 Dec 11.
Artigo em Zh | MEDLINE | ID: mdl-36480884

RESUMO

Objective: To evaluate the relationship between lesion features of cytomegalovirus (CMV) retinitis, including lesion size and degree of opacity of lesion borders, and aqueous CMV-DNA load in patients after allogeneic hematopoietic stem cell transplantation. Methods: It was a retrospective case series study. Data of patients diagnosed as CMV retinitis after allogeneic hematopoietic stem cell transplantation at the Department of Ophthalmology, Peking University Third Hospital from January 2018 to October 2020 were reviewed. Lesion size was detected by ultra-wide-field fundus image and measured by ImageJ, an image processing and analysis software. Opacity of lesion borders was evaluated by a masked reader. The CMV retinitis lesions were divided into 3 types according to the opacity degree of the border of the lesions: suspected active lesion mildly to moderately opacified lesion and severely opacified lesion. All eyes were diagnosed as active cytomegalovirus retinitis. CMV-DNA in aqueous humor was detected at the first visit and at the end of the induction period of antiviral drugs. CMV-DNA load was quantitatively detected by polymerase chain reaction after 100 µl of aqueous humor was extracted, and were converted to common logarithmic representations. Pearson correlation analysis was used to analyze the correlation between lesion area, opacity degree of lesion border and aqueous humor CMV-DNA load. Results: A total of 71 eyes from 46 patients were included and 26 was male and 20 was female. The age of the patients was 27(13, 33)years. The active lesion size of CMV retinitis was 40(12, 65) disc areas. Eight eyes (11.3%) had a suspected active lesion, 51 eyes (71.8%) had a mildly to moderately opacified lesion border, and 12 eyes (16.9%) had a severely opacified border. At the first visit, 67 eyes (94.4%) were CMV-positive, and the CMV-DNA load was 2.04×104 (6.24× 102, 1.48 ×105) copies/ml. After 2 weeks of induction therapy, the viral load was 2.47×102 (1.08× 10, 6.87 ×103) copies/ml. The correlation analysis showed that the CMV-DNA load in aqueous humor was significantly correlated with the lesion border opacity both at presentation and 2 weeks after intravitreal antiviral treatment (r=0.765, P<0.001; r=0.761, P<0.001), but was not related with the size of active fundus lesions (r=0.209, P=0.095; r=0.220, P=0.078). Conclusion: Degrees of lesion border opacity can reflect levels of aqueous viral load of CMV retinitis in patients after allogeneic hematopoietic stem cell transplantation and can become a useful measurement for investigation of CMV retinitis activity.


Assuntos
Retinite por Citomegalovirus , Transplante de Células-Tronco Hematopoéticas , Humanos , Feminino , Masculino , Carga Viral , Estudos Retrospectivos , DNA
5.
Zhonghua Jie He He Hu Xi Za Zhi ; 44(8): 723-728, 2021 Aug 12.
Artigo em Zh | MEDLINE | ID: mdl-34645139

RESUMO

Objective: To explore the clinicopathological characteristics and genomic characteristics of four patients with epidermal growth factor receptor(EGFR)-mutated advanced adenocarcinoma transformed into small-cell lung cancer. Methods: Four cases of EGFR-mutated advanced adenocarcinoma of the lung transformed into small-cell lung cancer were studied by clinical data, pathological morphology, immunohistochemistry and gene detection. Result: EGFR-mutated adenocarcinoma of the lung was heterogeneous in clinical and genomic profiles, of ten characterized by RB1, TP53 and PIK3CA mutations. Its transformation into small-cell lung cancer was a particularly aggressive mechanism of drug resistance, but the machanisms were not clear NSE and other tumor indicators had low diagnostic value for transformation. Conclusions: EGFR-mutated adenocarcinoma of the lung transformed into small-cell lung cancer was one of the reasons for EGFR resistance with avery poor prognosis.


Assuntos
Adenocarcinoma , Neoplasias Pulmonares , Adenocarcinoma/genética , Receptores ErbB/genética , Humanos , Neoplasias Pulmonares/genética , Mutação , Inibidores de Proteínas Quinases
6.
Zhonghua Wai Ke Za Zhi ; 59(3): 203-209, 2021 Mar 01.
Artigo em Zh | MEDLINE | ID: mdl-33685054

RESUMO

Objective: To investigate the efficacy and the safety of intravascular therapy for cerebrovascular ischemic tandem stenosis. Methods: Clinical data of 35 patients with symptomatic anterior circulation and posterior circulation tandem stenosis who received intravascular therapy for two sites of stenosis at the same time at Department of Neurosurgery of Peking University First Hospital from January 2013 to December 2018 were analyzed retrospectively. There were 27 males and 8 females,aged (65.6±9.4)years (range:47 to 81 years).There were 14 cases of anterior circulation tandem stenosis and 21 of posterior circulation tandem stenosis.The medical records were collected with emphasis on postoperative symptoms,imaging manifestations and modified Rankin scale(mRS) scores. Results: Sixty-eight stents were implants in to 35 patients,including 49 extracranial implants and 19 intracranial implants.The surgical success rate was 100%.The perioperative death rate was 0,and 1 patient(1/35,2.9%) had cerebral hemorrhage.All patients were followed up for 18 months.During 3 to 12 months after the intervention,1 case(1/35,2.9%) had stent restenosis,and 4 cases(4/35,11.4%) had persisted symptoms such as dizziness and weakness in limbs.All patients'mRS scores were ≤2. No new stroke occurred. During 12 to 18 months after the intervention,3 cases had in-stent restenosis,increasing the rate to 11.4% (4/35). The mRS scores of 32 patients(32/35,91.4%) were ≤2. Conclusion: Intravascular therapy for patients with symptomatic tandem stenosis is a feasible and safe procedure with good short-term outcomes.


Assuntos
Isquemia Encefálica/terapia , Doenças Arteriais Cerebrais/terapia , Constrição Patológica/terapia , Procedimentos Endovasculares , Stents , Idoso , Idoso de 80 Anos ou mais , Implante de Prótese Vascular , Isquemia Encefálica/etiologia , Procedimentos Endovasculares/instrumentação , Procedimentos Endovasculares/métodos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Resultado do Tratamento
7.
Phys Rev Lett ; 121(16): 167004, 2018 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-30387623

RESUMO

A magnetic order can be completely suppressed at zero temperature (T), by doping carriers or applying pressure, at a quantum critical point, around which physical properties change drastically. However, the situation is unclear for an electronic nematic order that breaks rotation symmetry. Here, we report nuclear magnetic resonance studies on NaFe_{1-x}Co_{x}As where magnetic and nematic transitions are well separated. The nuclear magnetic resonance spectrum is sensitive to inhomogeneous magnetic fields in the vortex state, which is related to London penetration depth λ_{L} that measures the electron mass m^{*}. We discovered two peaks in the doping dependence of λ_{L}^{2}(T∼0), one at x_{M}=0.027 where the spin-lattice relaxation rate shows quantum critical behavior, and another at x_{c}=0.032 around which the nematic transition temperature extrapolates to zero and the electrical resistivity shows a T-linear variation. Our results indicate that a nematic quantum critical point lies beneath the superconducting dome at x_{c} where m^{*} is enhanced. The impact of the nematic fluctuations on superconductivity is discussed.

8.
Fa Yi Xue Za Zhi ; 34(6): 659-664, 2018 06.
Artigo em Zh, Inglês | MEDLINE | ID: mdl-30896108

RESUMO

Due to the concealment and incidence higher than reported statistics, infanticide should attract the attention of legal medical experts. The infanticide process has common behavioral characteristics. The perpetrators are most frequently biological mothers who are unmarried, with inferior education, and lack of routine pregnancy tests. Medicolegal identification of infanticide cases includes identification of live births and causes of death. Besides the classical lung floating test, there are other methods to identify live births such as pulmonary interstitial emphysema, CT imaging examination, immunohistochemical examination of umbilical cord vitality markers. Neonatal line measurement is the only way to identify live births in corpse with severe decomposition. The main causes of death in infanticide are mechanic asphyxia and fatal head trauma. Shaken baby syndrome, as a common abusive head trauma, is pathologically characterized by the triad of encephalopathy, subdural haemorrhage (SDH) and retinal haemorrhage (RH). During the autopsy of suspected shaking baby syndrome, in addition to carefully examining cervical cord, the possibility of pathological temporal lobe hemorrhage should also be ruled out.


Assuntos
Traumatismos Craniocerebrais , Infanticídio , Síndrome do Bebê Sacudido , Traumatismos Craniocerebrais/etiologia , Medicina Legal , Hematoma Subdural , Humanos , Lactente , Hemorragia Retiniana/etiologia
9.
Genet Mol Res ; 16(2)2017 Apr 13.
Artigo em Inglês | MEDLINE | ID: mdl-28407193

RESUMO

Haematococcus pluvialis, as the most potential natural source of astaxanthin, which is a powerful antioxidant with high economic value, has attracted more and more scientific attention in recent years. An in-depth understanding of the mechanism for how H. pluvialis produces astaxanthin requires the intensive investigations on its genetic information. In particular, many reported studies were based on a variety of RNA analyses. However, it is difficult to extract RNA with high quality and quantity from H. pluvialis, because of the blockage from its thick cell wall and contamination by a large quantity of pigments, polysaccharides, and lipids. Therefore, we proposed an optimized Trizol-based RNA extraction method for H. pluvialis by investigating the effect of cell wall broken ways, algal strains, and cell growth status on total RNA isolation. Using this rapid, convenient, and cost-saving method, isolated H. pluvialis RNA had high quantity and quality (with an RNA integrity number of 7.0 and a concentration of 1604.1 ng/µL) equivalent to that isolated by commercial kit, enabling its applications into downstream RNA analyses.


Assuntos
Clorófitas/química , RNA de Plantas/química , Fracionamento Celular/métodos , Fracionamento Químico/métodos , RNA de Plantas/normas
10.
Zhonghua Yi Xue Za Zhi ; 97(30): 2372-2378, 2017 Aug 08.
Artigo em Zh | MEDLINE | ID: mdl-28822457

RESUMO

Objective: To evaluate the safety and efficacy of peripheral arterial disease patients with critical limb ischemia who accepted BSX or EVT. Methods: According to the requirements of systematic review, we searched MEDLINE (1980-2014), Emabse (1980-2014), Journals @ Ovid Full Text (1980-2014) databases, selected literature and extracted data, meta-analysis was performed through STATA11.2. Results: A total of 17 studies (3 RCTs, 14 non-randomized studies) and 5 515 patients (BSX group: 2 454, EVT group: 2 769) were deemed eligible. Meta-analysis showed there were no differences between the two groups in 30-day mortality (OR=1.110, P=0.523). However, BSX was significantly associated with increasing overall complications (OR=2.456, P=0.003), infections (OR=3.163, P<0.001) and thrombosis (OR=3.069, P=0.002), but showed a reduction in dissection and pseudoaneurysm (OR=0.537, P=0.012). During the follow-up, BSX had significant advantages. The 1-year and 3-year primary patency of BSX patients were higher than EVT group (1 year, OR=1.415, P=0.008; 3 years, OR=1.619, P<0.001); so were in secondary patency( 1 year, OR=2.156, P<0.001; 3 years, OR=2.547, P<0.001). Meanwhile, the 5-year overall survival rate was also higher in BSX group (OR=1.243, P=0.007). Conclusions: EVT has potential advantages in reducing surgical trauma and early postoperative complications, shortening hospital stay and so on. Concerning long-term results, BSX is better in reducing long-term mortality and improving long-term patencies than EVT group.


Assuntos
Isquemia , Doença Arterial Periférica , Amputação Cirúrgica , Angioplastia com Balão , Humanos , Estudos Retrospectivos , Fatores de Risco , Resultado do Tratamento
11.
Appl Microbiol Biotechnol ; 100(24): 10295-10306, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27459869

RESUMO

A strategy to supply molecular oxygen for microbial calcium precipitation was developed for the first time. Firstly, a controlled oxygen-releasing tablet (ORT) containing CaO2 and lactic acid with a suitable ratio of 9:1 was developed. It can provide a stable oxygen supply and maintain pH in the range of 9.5-11.0 for 45 days while contacting with water. In the presence of oxygen, a self-healing bacterium H4 spores germinated more effectively and maintained high metabolic activity. Furthermore, H4 vegetative cells induced 50 % more calcium precipitation than that obtained without oxygen supply. Finally, a binary self-healing system containing bacterial spores and ORT was established. The calcium precipitation experiments showed that H4 in the binary self-healing system precipitated 27.5 mM calcium with oxygen supply after 32 days and dissolved oxygen (DO) concentration of the solution decreased from 15 to 4 mg l-1, while only 6.9 mM calcium precipitation was obtained without oxygen supply. This work can disclose the effect of oxygen on microbial calcium precipitation and further lay a foundation for the establishment of ternary self-healing system containing bacteria, ORT, and nutrients, which will be promising for the self-healing of cracks deep inside the concrete structure.


Assuntos
Bacillus/metabolismo , Cálcio/metabolismo , Materiais de Construção/microbiologia , Oxigênio/metabolismo , Esporos Bacterianos/metabolismo , Comprimidos/metabolismo , Bacillus/crescimento & desenvolvimento , Precipitação Química , Concentração de Íons de Hidrogênio , Ácido Láctico/metabolismo , Esporos Bacterianos/crescimento & desenvolvimento
12.
Zhonghua Wai Ke Za Zhi ; 54(12): 891-893, 2016 Dec 01.
Artigo em Zh | MEDLINE | ID: mdl-27916029

RESUMO

Bypass surgery(BSX) and endovascular therapy(EVT) are the most important therapeutic method to critical limb ischemia.EVT has potential advantages in reducing surgical trauma and early postoperative complications, shortening hospital stay and so on. Concerning long-term results, BSX is better in reducing long-term mortality and improving long-term patency than EVT group. Therefore, control indications reasonably and select individualized methods, avoid the abuse of EVT are more meaningful for patients.


Assuntos
Doença Arterial Periférica/cirurgia , Enxerto Vascular , Idoso , Procedimentos Endovasculares , Feminino , Humanos , Isquemia , Salvamento de Membro , Masculino , Pessoa de Meia-Idade , Complicações Pós-Operatórias , Estudos Retrospectivos , Resultado do Tratamento
13.
Small ; 10(15): 3045-9, 2014 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-24729306

RESUMO

A simple, scalable method is reported to fabricate ordered hetero-structures of organic materials using template-directed growth. The 3D extension of the structures is firstly determined by pre-pattern size and deposition amount, and can further be in situ tuned by annealing at appropriate temperature.

14.
Poult Sci ; 92(9): 2326-32, 2013 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-23960114

RESUMO

We investigated the resistance of avian Escherichia coli to commonly used clinical antibiotics in Hebei Province. It is of significance to reveal the extent and mechanism of drug resistance, as well as to prevent and control colibacillosis. We investigated the resistance of 132 E. coli isolates to 5 kinds of antibiotics (including ß-lactams, aminoglycosides, tetracyclines, sulfonamides, and chloramphenicol) using the Kirby-Bauer drug susceptibility test, and resistance genes were detected by PCR. The results showed that the E. coli had a higher resistance rate to ampicillin, cephalotin, gentamicin, streptomycin, tetracycline, doxycycline, sulfamethoxazole trimethoprim, sulfamonomethoxine, and florfenicol, but it had higher sensitive rate to cefepime, imipenem, spectinomycin, and minocycline. Of the resistance genes, the TEM, aac(3)-IIa, tetA, Sul1, Sul2, and forl had higher positive rates. Therefore, drug resistance of avian E. coli in Hebei Province is very serious. The resistance mechanisms may include structure changes of the target enzyme mediated by multiresistance genes, and resulting in reduced affinity and increased efflux of antibiotics.


Assuntos
Antibacterianos/farmacologia , Galinhas , Farmacorresistência Bacteriana Múltipla , Infecções por Escherichia coli/veterinária , Escherichia coli/efeitos dos fármacos , Escherichia coli/genética , Doenças das Aves Domésticas/microbiologia , Animais , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , China , Infecções por Escherichia coli/microbiologia , Reação em Cadeia da Polimerase/veterinária
15.
Zhonghua Liu Xing Bing Xue Za Zhi ; 44(5): 689-693, 2023 May 10.
Artigo em Zh | MEDLINE | ID: mdl-37221054

RESUMO

A crucial lesson gained through the pandemic preparedness and response to COVID-19 is that all measures for epidemic control must be law-based. The legal system is related not only to public health emergency management per se but also to all aspects of the institutional supporting system throughout the lifecycle. Based on the lifecycle emergency management model, this article analyses the problems of the current legal system and the potential solutions. It is suggested that the lifecycle emergency management model shall be followed to establish a more comprehensive public health legal system and to gather the intelligence and consensus of experts with different expertise, including epidemiologists, sociologists, economists, jurist and others, which will collaboratively promote the science-based legislation in the field of epidemic preparedness and response for the establishment of a comprehensive legal system for public health emergency management and with Chinese characteristics.


Assuntos
Planejamento em Desastres , Saúde Pública , Humanos , China , Pandemias/prevenção & controle , Emergências
16.
Poult Sci ; 91(4): 884-7, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22399727

RESUMO

It is important for the prevention and treatment of colibacillosis to monitor drug resistance in Escherichia coli. To choose effective drugs to prevent and control avian colibacillosis in North China, we investigated the resistance of 164 E. coli isolates (from Beijing, Tianjin, Inner Mongolia, Shanxi, and Hebei regions of China) to commonly used clinical sulfonamide antibiotics using a drug susceptibility test. The results show that the rates of resistance to sulfamonomethoxine and sulfamethoxazole-trimethoprim were 90.24% (148/164) and 73.17% (120/164), respectively. To explore the reasons for wide drug resistance, sulfonamide resistance genes were detected by PCR. Of the isolates, 90.85% (149/164) carried sulfonamide resistance genes. Therefore, resistance of avian E. coli to sulfonamide antibiotics is very serious in North China, perhaps because of the existence of resistance genes, but other resistance mechanisms may also contribute to tolerogenesis.


Assuntos
Antibacterianos/farmacologia , Galinhas , Farmacorresistência Bacteriana/genética , Infecções por Escherichia coli/veterinária , Escherichia coli/genética , Doenças das Aves Domésticas/epidemiologia , Sulfonamidas/farmacologia , Animais , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Proteínas de Transporte/genética , Proteínas de Transporte/metabolismo , China/epidemiologia , Di-Hidropteroato Sintase/genética , Di-Hidropteroato Sintase/metabolismo , Eletroforese em Gel de Ágar , Escherichia coli/efeitos dos fármacos , Escherichia coli/isolamento & purificação , Infecções por Escherichia coli/epidemiologia , Infecções por Escherichia coli/microbiologia , Proteínas de Escherichia coli/genética , Proteínas de Escherichia coli/metabolismo , Genótipo , Testes de Sensibilidade Microbiana , Reação em Cadeia da Polimerase , Doenças das Aves Domésticas/microbiologia , Alinhamento de Sequência
17.
Poult Sci ; 91(11): 2774-7, 2012 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-23091131

RESUMO

The experiment was performed to investigate the tetracycline resistance and antibiotic-resistant genotype of avian Escherichia coli in North China and to analyze the correlation of genotype and phenotype. The resistance of 164 E. coli isolates (from Beijing, Tianjin, inner Mongolia, Shanxi, and Hebei regions of China) to tetracycline, doxycycline, and minocycline was investigated by using a drug susceptibility test. The results show that the rate of resistance to tetracycline antibiotics was 89.63% (147/164). The higher resistance rate was 84.76% (139/164) to tetracycline and 70.12% (115/164) to doxycycline, and the lowest resistance rate was 4.88% (8/164) to minocycline. The distribution of tetracycline resistance (Tcr) genes (tetA, tetB, tetC, and tetM) in avian E. coli isolates was detected by PCR. Of the isolates, 82.32% (135/164) carried tetracycline resistance genes. The positive rates of tetA, tetB, and tetM were 57.93% (95/164), 38.41% (63/164), and 10.97% (18/164), respectively. No tetC was amplified in avian E. coli isolates. The total positive rate of resistance genes (82.32%) was almost equal to the total rate of resistance to tetracycline antibiotics (89.63%). Thus, the positive rate of genotype was basically in line with that of phenotype for tetracycline resistance. The tetracycline resistance genes are widely distributed in E. coli and their main resistance mechanism to tetracycline is the active efflux effect mediated by tetA and tetB.


Assuntos
Galinhas , Infecções por Escherichia coli/veterinária , Escherichia coli/efeitos dos fármacos , Doenças das Aves Domésticas/microbiologia , Resistência a Tetraciclina/genética , Tetraciclina/farmacologia , Animais , Antibacterianos/uso terapêutico , China/epidemiologia , Escherichia coli/genética , Escherichia coli/isolamento & purificação , Infecções por Escherichia coli/epidemiologia , Infecções por Escherichia coli/microbiologia , Genótipo , Doenças das Aves Domésticas/epidemiologia
18.
Poult Sci ; 91(10): 2482-6, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22991531

RESUMO

Monitoring drug resistance in Escherichia coli is important for prevention and treatment of colibacillosis. To choose effective drugs to prevent and control avian colibacillosis in North China, we investigated resistance of 205 E. coli isolates (from Beijing, Tianjin, inner Mongolia, Shanxi, and Hebei regions) to commonly used clinical aminoglycoside antibiotics using a drug susceptibility test. The results show that the isolates had varying degrees of resistance to kanamycin, gentamicin, streptomycin, amikacin, neomycin, and spectinomycin. Particularly, the resistance rates of the former 3 antibiotics exceeded 40%. To explore the reasons for wide drug resistance, aminoglycosides modifying enzymes (AME) genes, which are important in generation of aminoglycoside resistance, were detected by PCR. Of the isolates, 60.98% carried AME genes and 38.05% carried commensal multidrug resistance genes. Therefore, resistance of avian E. coli to aminoglycoside antibiotics is very serious in North China, perhaps due to the existence of resistance genes.


Assuntos
Aminoglicosídeos/farmacologia , Antibacterianos/farmacologia , Farmacorresistência Bacteriana , Infecções por Escherichia coli/microbiologia , Escherichia coli/efeitos dos fármacos , Doenças das Aves Domésticas/microbiologia , Animais , Galinhas , China , Escherichia coli/genética , Infecções por Escherichia coli/epidemiologia , Genótipo , Epidemiologia Molecular , Doenças das Aves Domésticas/epidemiologia
19.
Eur Rev Med Pharmacol Sci ; 25(5): 2172-2181, 2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-33755954

RESUMO

Exosomes, small membrane vesicles with a diameter of 30-100 nm, transport lipids, proteins, DNA, and RNA. Exosomes originate from endocytic vessels and are processed and released through exocytosis. They can be taken up by target cells and mediate intercellular communication. Initially, exosomes were thought to be waste products excreted by cells. However, with more research, they have been found to play important roles in physiological and pathological processes. Therefore, they are promising biomarkers for the diagnosis and treatment of a variety of disease conditions, including fundus diseases, ocular surface diseases, retinal diseases, tumors, ocular trauma, and light damage. In this review, we discuss the history, biogenesis, release, isolation, characterization, and biological functions of exosomes, as well as their future application prospects in ophthalmic diseases.


Assuntos
Oftalmopatias/sangue , Glaucoma/sangue , Melanoma/sangue , Neoplasias Uveais/sangue , Exossomos/metabolismo , Humanos
20.
Eur Rev Med Pharmacol Sci ; 25(21): 6539-6547, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34787856

RESUMO

Cytoplasmic dynein is a multi-subunit complex that includes cytoplasmic dynein-1 (dynein1) and cytoplasmic dynein-2 (dynein2). It participates in various basic cellular processes, including nuclear migration, mitotic spindle organization, chromosome separation during mitosis, and the location and function of numerous intracellular organelles. Retinal photoreceptor cells are terminally differentiated neurons that cannot regenerate and cannot be replaced once lost. It is thus crucial to study their development to facilitate the generation and improvement of photoreceptor disease treatments. The outer segment (OS) of photoreceptor cells is a specific sensory cilium. An increasing number of studies have shown that cytoplasmic dynein plays an essential role in the development of retinal photoreceptor cells. To date, people have done a lot of studies on the various functions of dynein in cells and have a very detailed understanding. However, the role of dynein in retinal photoreceptor cells has not been summarized in detail. This article summarizes the currently available knowledge relating to the effects and mechanisms of cytoplasmic dynein on the development and functional maintenance of retinal photoreceptor cells.


Assuntos
Dineínas do Citoplasma/metabolismo , Células Fotorreceptoras de Vertebrados/metabolismo , Animais , Humanos
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