FT-ICR-MS combined with fluorescent spectroscopy reveals the driving mechanism of the spatial variation in molecular composition of DOM in 22 plateau lakes.

Dissolved organic matter (DOM) is the largest carbon pool and directly affects the biogeochemistry in lakes. In the current study, fourier transform ion cyclotron mass spectrometry (FT-ICR-MS) combined with fluorescent spectroscopy was used to assess the molecular composition and driving mechanism of DOM in 22 plateau lakes in Mongolia Plateau Lakes Region (MLR), Qinghai Plateau Lakes Region (QLR) and Tibet Plateau Lakes Region (TLR) of China. The limnic dissolved organic carbon (DOC) content ranged from 3.93 to 280.8 mg L-1 and the values in MLR and TLR were significantly higher than that in QLR. The content of lignin was the highest in each lake and showed a gradually decreasing trend from MLR to TLR. Random forest model and structural equation model implied that altitude played an important role in lignin degradation while the contents of total nitrogen (TN) and chlorophyll a (Chl-a) have a great influence on the increase of DOM Shannon index. Our results also suggested that the inspissation of DOC and the promoted endogenous DOM production caused by the inspissation of nutrient resulted in a positive relationship between limnic DOC content and limnic factors such as salinity, alkalinity and nutrient concentration. From MLR to QLR and TLR, the molecular weight and the number of double bonds gradually decreased but the humification index (HIX) also decreased. In addition, from the MLR to the TLR, the proportion of lignin gradually decreased, while the proportion of lipid gradually increased. Both above results suggested that photodegradation was dominated in lakes of TLR, while microbial degradation was dominated in lakes of MLR.

The combined toxicity of binary mixtures of antibiotics against the cyanobacterium Microcystis is dose-dependent: insight from a theoretical nonlinear combined toxicity assessment method.

The entry of antibiotics into aquatic ecosystems has a serious impact. Antibiotics usually exist as mixtures in natural water bodies. Therefore, it is particularly important to evaluate the mixed toxicity of antibiotic mixtures. The study of the combined toxicity of binary mixtures of antibiotics is the basis for exploring the mixed toxicity of multiple antibiotics. In this investigation, Microcystis aeruginosa (M. aeruginosa) was used as the test organism, and a theoretical nonlinear combined toxicity assessment method was adopted to evaluate the effects of binary mixtures of antibiotics consisting of tetracycline (TC), sulfadiazine (SD), and sulfamethoxazole (SMX) on cell growth, enzymatic activity, and gene expression. The median lethal concentrations of TC, SD, and SMX to M. aeruginosa were 0.52 mg L-1, 1.65 mg L-1, and 0.71 mg L-1, respectively. The results from the theoretical nonlinear combined toxicity assessment method showed that SD + TC was synergistic at low concentrations and antagonistic at high concentrations, while the combinations of SMX + SD and SMX + TC were synergistic. The determination of enzymatic activity and gene expression indicated that the antibiotics could inhibit the growth of M. aeruginosa by destroying the cell membrane structure, inhibiting photosynthesis, impeding the cell division process and the electron transfer process, and destroying the molecular structure of proteins and DNA. Different combinations of antibiotics have different degrees of damage to the antioxidant system and cell membrane self-repair function of M. aeruginosa, which are the reasons for the different combined toxicity effects.