Trophic ecology of co-occurring fishes in the Sundays River Valley irrigation ponds, assessed using stable isotope and gut content analyses.

The analysis of food web structures has increased the understanding of the dynamics of organisms belonging to different trophic levels. In this study, the diet of two native species, Glossogobius callidus and Gilchristella aestuaria, was assessed in the presence of two non-native species, Oreochromis mossambicus and Gambusia affinis, in irrigation ponds, Eastern Cape Province, South Africa. The proportion of dietary items consumed and assimilated by the four fish species were inferred from gut contents and carbon and nitrogen stable isotope analysis. Stable isotope analysis revealed that both G. affinis and O. mossambicus had a larger isotopic niche size than G. callidus and G. aestuaria. Although G. callidus fed on benthic resources and G. aestuaria fed on phytoplankton, gut content analysis showed that G. callidus, O. mossambicus and G. affinis fed predominantly on benthic resources, whereas G. aestuaria fed mainly on plankton resources. Considerable niche overlap corroborates the view that resource competition is a major factor shaping the composition of the four fish species. This study highlighted the low diversity of the food web within the Sundays River Valley irrigation ponds, where food items are shared by all the small-bodied fishes.

Thermal limits and preferences of large branchiopods (Branchiopoda: Anostraca and Spinicaudata) from temporary wetland arid zone systems.

Large branchiopods are specialist crustaceans adapted for life in temporary, thermally dynamic wetland ecosystems. Certain large branchiopod species are, however, restricted to specific temporary wetland types, exemplified by their physico-chemical and hydroperiod characteristics. Here, we contrasted the thermal preference and critical thermal maxima (CTmax) and minima (CTmin) of southern African anostracans and spinicaudatans found exclusively in either temporary rock-pool or pan wetland types. We hypothesized that environment of origin would be a good predictor of thermal preference and critical thermal limits. To test this, Branchiopodopsis tridens (Anostraca) and Leptestheria brevirostris (Spinicaudata) were collected from rock-pool habitats, while Streptocephalus cafer (Anostraca) and a Gondwanalimnadia sp. (Spinicaudata) were collected from pan habitats. In contrast to our hypothesis, taxonomic relatedness was a better predictor of CTmax and temperature preference than environment of origin. Spinicaudatans were significantly more tolerant of high temperatures than anostracans, with L. brevirostris and Gondwanalimnadia sp. median CTmax values of 45.1 °C and 44.1 °C, respectively, followed by S. cafer (42.8 °C) and B. tridens (41.4 °C). Neither environment or taxonomic relatedness were good predictors of CTmin trends, with B. tridens (0.9 °C) and Gondwanalimnadia sp. (2.1 °C) having the lowest median CTmin values, followed by L. brevirostris (3.4 °C) and S. cafer (3.6 °C). On the contrary, temperature preferences differed according to taxa, with spinicaudatans significantly preferring higher temperatures than anostracans. Leptestheria brevirostris and Gondwanalimnadia sp. both spent most time at temperatures 30-32 °C, S. cafer at 18-20 °C and B. tridens at 21-23 °C. Constrained thermal traits reported here suggest that the studied anostracans might be more susceptible to projected climatic warming than the spinicaudatans, irrespective of habitat type, however, these taxa may also compensate through phenotypic plasticity.

Spatial Anopheles arabiensis (Diptera: Culicidae) insecticide resistance patterns across malaria-endemic regions of Botswana.

BACKGROUND: Since the advent of the Green Revolution, pesticides have played an important role in the global management of invertebrate pests including vector mosquitoes. Despite optimal efficacy, insects often display insensitivity to synthetic insecticides owing to prolonged exposure that may select for resistance development. Such insecticide insensitivity may regress national and regional coordination in mosquito vector management and indeed malaria control. In Botswana, prolonged use of synthetic insecticides against malaria vectors have been practiced without monitoring of targeted mosquito species susceptibility status. METHODS: Here, susceptibility status of a malaria vector (Anopheles arabiensis), was assessed against World Health Organization-recommended insecticides, across three malaria endemic districts. Adult virgin female mosquitoes (2-5 days old) emerging from wild-collected larvae were exposed to standardized insecticide-impregnated papers with discriminating doses. RESULTS: The results showed resistance dynamics were variable in space, presumably as a result of spatial differences in insecticide use across malaria endemic districts and the types of insecticides used in the country. Overall, there was a reduced susceptibility of An. arabiensis for the pyrethroid lambda-cyhalothrin and for dichloro diphenyl trichloroethane [DDT], which have similar modes of action and have been used in the country for many years. The Okavango district exhibited the greatest reduction in susceptibility, followed by Ngamiland and then Bobirwa, reflective of national intervention strategy spraying intensities. Vector mosquitoes were, however, highly susceptible to carbamates and organophosphates irrespective of region. CONCLUSIONS: These results provide important findings of vector susceptibility to insecticides recommended for vector control. The results highlight the need to implement insecticide application regimes that more effectively including regionally integrated resistance management strategies for effective malaria control and elimination.

Life history of the river goby Glossogobius callidus (Teleostei: Gobiidae).

The river goby Glossogobius callidus is native to freshwater and estuarine habitats in South Africa. Individuals [21.1-144.4 mm total length (LT )] were sampled from impoundments in the Sundays River Valley, Eastern Cape, from February 2014 to March 2015. The largest female was 137.2 mm LT , and the largest male was 144.4 mm LT . Length-at-50% maturity was 75.2 ± 2.1 mm LT for males and 76.2 ± 2.0 mm LT for females. Absolute fecundity was 1028.2 ± 131.7 oocytes per fish, and relative fecundity was 50.1 ± 18.1 oocytes per gram. The spawning season extended from October to December. Fish were aged using sectioned sagittal otoliths. The growth zone periodicity was validated using edge analysis. Longevity was more than 7 years for females and more than 6 years for males. Length-at-age was similar for the two sexes and was best described using the von Bertalanffy growth model as Lt = 74.7(1 - e-1.0(t + 0.1) ) mm LT for the entire population. Using the population age structure, the mortality rate was estimated at 1.3 per year.

Agricultural intensification and drought frequency increases may have landscape-level consequences for ephemeral ecosystems.

Ephemeral wetlands in arid regions are often degraded or destroyed through poor land-use practice long before they are ever studied or prioritized for conservation. Climate change will likely also have implications for these ecosystems given forecast changes in rainfall patterns in many arid environments. Here, we present a conceptual diagram showing typical and modified ephemeral wetlands in agricultural landscapes and how modification impacts on species diversity and composition.

Water and sediment chemistry drivers of chlorophyll-a dynamics within a Ramsar declared floodplain pan wetland system.

Floodplain pans are hydrologically dynamic in nature and characterised by variables such as chlorophyll-a (chl-a), water, and sediment chemistry over their hydroperiods. The present study investigated the spatio-temporal variations in water and sediment physico-chemical, and chlorophyll-a concentration characteristics of six floodplain pans found in the Ramsar declared Makuleke wetlands, Kruger National Park, South Africa. The water and sediment physico-chemical variable values were generally elevated during the high-water period, whereas chlorophyll-a concentrations varied across pans and hydroperiod. Benthic chl-a concentration significantly varied across pans with concentrations ranging from 161 to 1036.2 mg m2. The two-way ANOVA showed significant differences in benthic chl-a concentration among hydroperiods, and no significant differences were observed in pelagic chl-a across pans and hydroperiods. Generally, pelagic and benthic chl-a concentration increased as water and sediment chemistry variables increased. Furthermore, three sediment variables, i.e. pH, calcium, and magnesium, and water conductivity were found to be significant in structuring benthic chlorophyll-a dynamics in pans. However, none of the sediment and water variables had a significant effect on pelagic chl-a. Hydroperiod had a significant effect on influencing chl-a concentration, with high and low water level periods being characterised by low and high chl-a concentration, respectively. The n-MDS results showed strong overlaps in chl-a biomass among the Makuleke floodplain pans across hydroperiods. The increasing chl-a concentration in these floodplain pans due to potential bioturbation effects as a result of large mammals could potentially lead to eutrophication, which in turn could affect the system's primary productivity and aquatic biota. Therefore, it is important to establish a continuous monitoring programme on these pans to inform sound management decisions.

Microbial community and extracellular polymeric substance dynamics in arid-zone temporary pan ecosystems.

Microbial extracellular polymeric substances (EPS) are an important component in sediment ecology. However, most research is highly skewed towards the northern hemisphere and in more permanent systems. This paper investigates EPS (i.e., carbohydrates and proteins) dynamics in arid Austral zone temporary pans sediments. Colorimetric methods and sequence-based metagenomics techniques were employed in a series of small temporary pan ecosystems characterised by alternating wet and dry hydroperiods. Microbial community patterns of distribution were evaluated between seasons (hot-wet and cool-dry) and across depths (and inferred inundation period) based on estimated elevation. Carbohydrates generally occurred in relatively higher proportions than proteins; the carbohydrate:protein ratio was 2.8:1 and 1.6:1 for the dry and wet season respectively, suggesting that EPS found in these systems was largely diatom produced. The wet- hydroperiods (Carbohydrate mean 102 µg g-1; Protein mean 65 µg g-1) supported more EPS production as compared to the dry- hydroperiods (Carbohydrate mean 73 µg g-1; Protein mean 26 µg g-1). A total of 15,042 Unique Amplicon Sequence Variants (ASVs) were allocated to 51 bacterial phyla and 1127 genera. The most abundant genera had commonality in high temperature tolerance, with Firmicutes, Actinobacteria and Proteobacteria in high abundances. Microbial communities were more distinct between seasons compared to within seasons which further suggested that the observed metagenome functions could be seasonally driven. This study's findings implied that there were high levels of denitrification by mostly nitric oxide reductase and nitrite reductase enzymes. EPS production was high in the hot-wet season as compared to relatively lower rates of nitrification in the cool-dry season by ammonia monooxygenases. Both EPS quantities and metagenome functions were highly associated with availability of water, with high rates being mainly associated with wet- hydroperiods compared to dry- hydroperiods. These data suggest that extended dry periods threaten microbially mediated processes in temporary wetlands, with implications to loss of biodiversity by desiccation.

Assessing chlorophyll-a and water quality dynamics in arid-zone temporary pan systems along a disturbance gradient.

Temporary pans are susceptible to various anthropogenic effects such as pollution, resource extraction, and land use intensification. However, given their small endorheic nature, they are almost entirely influenced by activities close to their internally drained catchments. Human-mediated nutrient enrichment within the pans can lead to eutrophication, resulting in increased primary productivity and decreased associated alpha diversity. The Khakhea-Bray Transboundary Aquifer region and the pan systems that characterise the area are understudied area with no records available of the biodiversity therein. Additionally, the pans are a major water source for the people in these areas. This study assessed differences in nutrients (i.e., ammonium, phosphates) and their effect on chlorophyll-a (chl-a) concentrations in pans along a disturbance gradient in the Khakhea-Bray Transboundary Aquifer region, South Africa. Physicochemical variables, nutrients, and chl-a were measured from 33 pans representing variable anthropogenic exposure during the cool-dry season in May 2022. Five environmental variables (i.e., temperature, pH, dissolved oxygen, ammonium, and phosphates) showed significant differences between the undisturbed and disturbed pans. The disturbed pans generally had elevated pH, ammonium, phosphates and dissolved oxygen compared to the undisturbed pans. A strong positive relationship was observed between chl-a and temperature, pH, dissolved oxygen, phosphates and ammonium. Chlorophyll-a concentration increased as surface area, and the distance from kraals, buildings and latrines decreased. Anthropogenic activities were found to have an overall effect on the pan water quality within the Khakhea-Bray Transboundary Aquifer region. Therefore, continuous monitoring strategies should be established to better understand the nutrient dynamics through time and the effect that this may have on productivity and diversity in these small endorheic systems.

Limited Effect of Ground Floor Fogging on Mosquito Distribution in High-Rise Condominia.

Fogging with insecticides is one of the main control measures for adult mosquito populations employed in countries that are affected by dengue. In many such countries, urban communities are increasingly characterised by high-density residence in high-rise condominia. Although fogging is typically applied at the ground level, its efficacy in three-dimensional urban environments is poorly understood. Here, we investigated the effect of fogging on vector mosquito distribution and abundance in high-rise condominia by conducting a before-after fogging survey. We showed that although mosquitoes were significantly concentrated at the lower levels in high-rise condominia, they were found throughout the three-dimensional environments. Fogging did not significantly alter this distribution or abundance pattern across any floor level. Thus, any fogging effect was short-lived as mosquito populations recovered within a few days before the subsequent scheduled treatment. In addition, increasing fogging frequency within practicable limits did not prolong the intended control effect. As urban mosquitoes are increasingly insusceptible to fogging due to insecticide resistance and vertical avoidance, this study demonstrates the need to implement other mosquito control strategies for high-rise condominia to manage mosquito populations.

Macroinvertebrate diversity in relation to limnochemistry in an Austral semi-arid transboundary aquifer region pan system.

Pan-wetland systems are one of the world's essential and productive ecosystems and are considered important, unique and complex ecosystems. Anthropogenic activities around the temporary pans in the Khakhea Bray Transboundary Aquifer region are increasingly becoming a big issue of concern as this may affect pan biodiversity. The study specifically aimed to investigate spatial and temporal distributions of metal and nutrient concentrations within the pans in relation to land use, identify potential pollution sources in this water-scarce region, and assess macroinvertebrate diversity and distribution in relation to pan limnochemistry using a combination of multivariate analyses from 10 pans across three seasons. Environmental and anthropogenic variables influence water quality and the distribution of metals concentration in Khakhea-Bray pan systems. Anthropogenic activities such as animal grazing, infrastructure degradation, water withdrawal and littering have resulted in poor water quality within temporary pans, which may strongly influence macroinvertebrate diversity and distribution. Forty-one macroinvertebrate species from 5 insect orders (i.e., Coleoptera, Hemiptera, Odonata, Ephemeroptera, Diptera), Crustacea and Mollusca were identified. Significant differences were observed across the seasons for macroinvertebrate taxa, with high and low species richness being observed in autumn and winter, respectively. Water (i.e., temperature, dissolved oxygen, pH, salinity, conductivity), physical (i.e., stone composition) and sediment (i.e., sulphur, sodium) parameters were found to have a significant impact on the macroinvertebrate communities. Therefore, understanding the relationships between macroinvertebrates and their environment is crucial in understanding how the ecosystem taxa are structured and is vital for informing conservation managers on how to properly manage and protect these systems from further degradation.

Pesticides drive differential leaf litter decomposition and mosquito colonisation dynamics in lentic conditions.

Global contamination of freshwater ecosystems by chemical compounds, such as pesticides, may exert high pressure on biologically-driven organic matter decomposition. These pollutants may also impair the quality of organic substrates for colonising invertebrates and reduce primary productivity by decreasing the abundance of phytoplankton. In southern Africa, increasing pesticide usage associated with macadamia plantations, in particular, presents a growing risk to freshwater ecosystems. Here, we examined macadamia (Macadamia integrifolia) leaf litter decomposition following exposure to three pesticides (i.e., Karate Zeon 10 CS (lambda-cyhalothrin), Mulan 20 AS (acetamiprid), Pyrinex 250 CS (chlorpyrifos)) used commonly in macadamia plantations, via an ex-situ microcosm approach. We examined mosquito colonisation of these microcosms as semi-aquatic macroinvertebrates which form a significant component of aquatic communities within standing waters. Macadamia leaf litter tended to decompose faster when exposed to Karate and Pyrinex pesticide treatments. Additionally, chlorophyll-a, conductivity, total dissolved solids, and pH differed among pesticide treatments and controls, with pesticides (Karate Zeon and Mulan) tending to reduce chlorophyll-a concentrations. Overall, pesticide treatments promoted mosquito (i.e., Culex spp.) and pupal abundances. In terms of dominant aquatic mosquito group abundances (i.e., Anopheles spp., Culex spp.), the effect of pesticides differed significantly among pesticide types, with Pyrinex and Mulan treatments having higher mosquito abundances in comparison to Karate Zeon and pesticide-free treatments. These findings collectively demonstrate that common pesticides used in the macadamia plantation may exert pressure on adjacent freshwater communities by shaping leaf-litter decomposition, semi-aquatic macroinvertebrate colonisation dynamics, and chlorophyll-a.

Community structure and environmental factors affecting diatom abundance and diversity in a Mediterranean climate river system.

Mediterranean climate river systems are among the most threatened ecosystems worldwide, due to a long history of anthropogenic impacts and alien invasive species introductions. Many of such rivers naturally exhibit a non-perennial flow regime, with distinct seasonal, inter-annual and spatial heterogeneity. The present study seeks to detect diatom community patterns and to understand the processes that cause these structures in an Austral Mediterranean river system among different months and river sections. In general, most environmental variables showed an increasing trend downstream for both months, with the exception of pH, dissolved oxygen, PO43- and substrate embeddedness, which decreased downstream. A total of 110 diatom species between the two study months (October - 106 taxa; January - 78 taxa) were identified, dominated by 30 species with at least >2% abundance. Diatom community structure differed significantly across river zones, while no significant differences were observed between the study months. A boosted regression trees model showed that B (43.3%), Cu (20.8%), Fe (3.4%) and water depth (3.2%) were the most significant variables structuring diatoms. Diatom species communities reflected environmental variables (i.e., sediment and water chemistry) in this Mediterranean climate river system, as sediment metals such as B, Cu and Fe were found to be important in structuring diatom communities. Biotic influences from fish communities had little effect on diversity, but shifted diatom community structure. Therefore, the current study highlights how river systems have complex interactions that play an important role in determining diatom species composition.

Drivers of aquatic macroinvertebrate communities in a Ramsar declared wetland system.

Wetlands in the Global South are under increasing pressure due to multiple stressors associated with global change. Water and sediment quality assessments, as well as biomonitoring using macroinvertebrate communities, are fundamental tools for informing wetland condition and management strategies. Here, we examine water and sediment parameters affecting aquatic macroinvertebrates in Nlyslvey Wetland, Limpopo Province, South Africa. Water quality, sediments, fish and macroinvertebrate community data were collected across three seasons (hot-dry, hot-wet, cool-dry) from five sites. Baetidae, Corixidae, Coenogrionidae, Dytiscidae and Physidae were the most abundant and dominant families, with functional feeding group (FFG) ratios indicating that all sites were strongly autotrophic, had high predator-prey ratios, few shredders and had a stable substrate across seasons. Fish abundances increased significantly towards the cool-dry season. Based on redundancy analysis, P, Ca, pH, Cu and Na were strongly positively associated with macroinvertebrates, including Physidae, Corixidae, Planorbidae, Ostracoda, Potamonautidae and Hydropyschidae; turbidity and sulphur were associated with Dytiscidae, Oligochaetae, Libellulidae, Gerridae and Dixidae; and fish abundance, Fe, oxygen reduction potential and total dissolved solids were negatively associated with Baetidae, Belostomatidae, Hydrophilidae and Leptoceridae. Therefore, these variables accounted for high levels of variation in macroinvertebrate families, with the cool-dry season clearly distinguished from the hot-wet and hot-dry seasons according to functional feeding groups. Being a protected area, this information could provide a useful baseline for further studies into wetlands in the region subject to greater anthropogenic stresses, as well as future studies in this Ramsar site. Further studies are required to assess the importance of environmental factors influencing the richness and distribution of macroinvertebrate communities in wetlands under growing anthropogenic pressures.

Land use effects on water quality, habitat, and macroinvertebrate and diatom communities in African highland streams.

Anthropogenic activities have increasingly subjected freshwater ecosystems globally to various pressures. Increasing land use activities have been highly linked to deteriorating freshwater ecosystems and dwindling biodiversity. For sound management and conservation policies to be implemented, relations between land use, environmental, and biotic components need to be widely documented. To evaluate the impacts of land use on biotic components, this study analyzed the diatom and macroinvertebrate community composition of the Eastern Highlands (Zimbabwe) streams to assess the main spatial diatom and macroinvertebrate community variances and how environmental variables and spatial factors influence community composition. Diatom and macroinvertebrate sampling was done in 16 streams in protected areas (national parks) and impacted sites (timber plantation and communal areas). Water (pH, phosphorus, and ammonium) and sediment (nitrogen, phosphorus, calcium, magnesium, manganese, and zinc) and habitat (substrate embeddedness, and habitat) variables differed significantly with land use. Principal Component Analysis (PCA) showed that the protected area had the best water quality, particularly marked by high pH levels and low phosphorus concentrations among environment types. Heavy metals were high in the communal areas, although mercury was higher in the national park. Significant differences were observed in diatom metrics, specifically dominance and evenness, with no significant differences observed in macroinvertebrate metrics across land uses. Diatoms differed in terms of composition in response to land use. Results provide an important scientific reference for land use optimization and guidance for the formulation of policies to protect freshwater resources in African Highland streams. Management and conservation initiatives in the Eastern Highlands are further recommended as this study detected high levels of mercury in the protected area, implying high levels of illegal mining.

Invasive carp alter trophic niches of consumers and basal resources in African reservoirs.

Environmental pollution and biological invasions are key drivers of biodiversity change. However, the effects of invasion and pollution on food webs remain largely unexplored. Here, we used stable isotopes to examine the effects of common carp Cyprinus carpio and pollution on trophic dynamics in six small reservoirs. Our results revealed that the trophic niche widths of invertebrates, vertebrates, and invasive carp did not significantly differ among reservoirs with different pollution statuses. However, we found low niche conservatism among reservoirs, suggesting that while niche width may remain consistent, there is a shift in the position of the niches in isotopic space under both pollution and invasion scenarios. Niche conservatism among reservoirs was generally higher in invertebrates, but this was also regardless of reservoir condition (i.e. presence or absence of pollution and invasion). These results suggest that invasion by species coupled with organic pollution may cause subtle yet differing effects on components of a food web (basal end-members, invertebrates and vertebrates). Our findings provide a baseline measure of the potential in the development of detection and response strategies for carp invasions and organic pollution.

Informing spread predictions of two alien snails using movement traits.

Invasive alien species are a growing global problem, and aquatic ecosystems have been regarded as particularly vulnerable. Biological invasions can alter ecosystem functioning, threaten native biodiversity and burden the global economy. Understanding alien species ability to disperse via locomotion following arrival to new environments is critical for prediction of spread rates. Here, we quantified in-field densities and compared movement traits between two widespread invasive alien snails, Tarebia granifera and Physa acuta. We measured the: (i) net distance and velocity to determine dispersal potential; and (ii) turning angles (both absolute and relative) and straightness index as proxies for exploratory behaviour. Tarebia granifera exhibited a significantly greater velocity and covered a significantly larger net distance (i.e., greater spread rate) than Physa acuta. In-field densities were marked for both species (T. granifera: mean 351 individuals m-2; P. acuta: mean 235 individuals m-2), but differed spatially. The exploratory behavior (i.e., mean or absolute turning angles and straightness index) did not differ significantly between the two alien species; both species showed a slight tendency to turn counterclockwise. The present study suggests a more rapid capacity to self-disperse in T. granifera than P. acuta, which could facilitate rapid spread within and between aquatic systems. Thus, this current study highlights the often-overlooked role of animal behaviour in promoting invasion; this autecological information can help inform predictive models for the spread of alien snails within freshwater ecosystems.

Disease Vector Relative Spatio-Temporal Abundances to Water Bodies and Thermal Fitness Across Malaria Endemic Semi-Arid Areas.

The biophysical environment plays an important role in the spatio-temporal abundance and distribution of mosquitoes. This has implications for the spread of vectors and diseases they cause across diverse landscapes. Here, we assessed vector mosquito abundances in relation to large water bodies, from three malaria districts in a semi-arid environment. Furthermore, we explored thermal limits to activity of the dominant and most medically important malaria vector across malaria-endemic areas. Mosquitoes were trapped near permanent water bodies across different districts. Critical thermal limits (critical thermal-maxima and -minima) to activity of wild adults and 4th instar larvae Anopheles arabiensis (Diptera: Culicidae) were assessed. Our results showed that Anopheles spp. dominate mosquito communities across all three districts, but that their numbers were far greater in Okavango than in other regions. At the Okavango sites, the numbers of Anopheles spp. decreased with distance from main water source. Anopheles spp. sampled in this region comprised Anopheles gambiae (Giles,1902) and Anopheles funestus (Giles, 1900) species complexes, with the former dominating in numbers. Thermal activity assays showed An. arabiensis females had wider thermal tolerance windows than males while larval thermal activity limits differed significantly across space. These results confirm that the Okavango district should be prioritized for vector control measures. Moreover, intervention strategies should consider recommendations for proximity effects to large water bodies, given the differential risk associated with distance from water. The wider thermal window on female vectors has implications for possible future malaria transmission and diverse habitat utilization under changing environments.